UniProtKB - P31695 (NOTC4_MOUSE)
Protein
Neurogenic locus notch homolog protein 4
Gene
Notch4
Organism
Mus musculus (Mouse)
Status
Functioni
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May regulate branching morphogenesis in the developing vascular system.By similarity1 Publication
GO - Molecular functioni
- calcium ion binding Source: InterPro
- Notch binding Source: MGI
- signaling receptor activity Source: InterPro
GO - Biological processi
- branching involved in blood vessel morphogenesis Source: UniProtKB
- endothelial cell differentiation Source: MGI
- endothelial cell morphogenesis Source: MGI
- epithelial to mesenchymal transition Source: MGI
- mammary gland development Source: MGI
- morphogenesis of a branching structure Source: UniProtKB
- negative regulation of cell adhesion molecule production Source: MGI
- negative regulation of cell-cell adhesion mediated by cadherin Source: MGI
- negative regulation of endothelial cell differentiation Source: UniProtKB
- negative regulation of Notch signaling pathway Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- Notch signaling pathway Source: UniProtKB
- positive regulation of angiogenesis Source: MGI
- positive regulation of aorta morphogenesis Source: MGI
- regulation of Notch signaling pathway Source: MGI
- regulation of protein localization Source: MGI
- regulation of protein processing Source: MGI
- vasculature development Source: UniProtKB
- venous blood vessel morphogenesis Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, Receptor |
Biological process | Differentiation, Notch signaling pathway, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-1912420, Pre-NOTCH Processing in Golgi R-MMU-350054, Notch-HLH transcription pathway R-MMU-9013700, NOTCH4 Activation and Transmission of Signal to the Nucleus R-MMU-9604323, Negative regulation of NOTCH4 signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Neurogenic locus notch homolog protein 4By similarityShort name: Notch 4 Cleaved into the following 3 chains: |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107471, Notch4 |
Subcellular locationi
Plasma membrane
Nucleus
Note: Following proteolytical processing NICD is translocated to the nucleus.
Cytosol
- cytosol Source: Reactome
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Nucleus
- nucleoplasm Source: Reactome
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- cell surface Source: UniProtKB
- cytoplasmic vesicle Source: MGI
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 1443 | ExtracellularSequence analysisAdd BLAST | 1423 | |
Transmembranei | 1444 – 1464 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1465 – 1964 | CytoplasmicSequence analysisAdd BLAST | 500 |
Keywords - Cellular componenti
Cell membrane, Membrane, NucleusPathology & Biotechi
Involvement in diseasei
Loss of the extracellular domain causes constitutive activation of the Notch protein, which leads to hyperproliferation of glandular epithelial tissues and development of mammary carcinomas.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1463 | V → L: NICD processing severely reduced. 1 Publication | 1 |
Keywords - Diseasei
Proto-oncogenePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000007704 | 21 – 1964 | Neurogenic locus notch homolog protein 4Add BLAST | 1944 | |
ChainiPRO_0000007705 | 1411 – 1964 | Transforming protein Int-3Add BLAST | 554 | |
ChainiPRO_0000007706 | 1428 – 1964 | Notch 4 extracellular truncationAdd BLAST | 537 | |
ChainiPRO_0000007707 | 1463 – 1964 | Notch 4 intracellular domainAdd BLAST | 502 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 25 ↔ 38 | By similarity | ||
Disulfide bondi | 32 ↔ 48 | By similarity | ||
Disulfide bondi | 50 ↔ 59 | By similarity | ||
Disulfide bondi | 65 ↔ 77 | By similarity | ||
Disulfide bondi | 71 ↔ 100 | By similarity | ||
Disulfide bondi | 102 ↔ 111 | By similarity | ||
Disulfide bondi | 119 ↔ 130 | By similarity | ||
Disulfide bondi | 124 ↔ 140 | By similarity | ||
Disulfide bondi | 142 ↔ 151 | By similarity | ||
Disulfide bondi | 157 ↔ 168 | By similarity | ||
Disulfide bondi | 162 ↔ 177 | By similarity | ||
Disulfide bondi | 179 ↔ 188 | By similarity | ||
Disulfide bondi | 195 ↔ 208 | By similarity | ||
Disulfide bondi | 202 ↔ 217 | By similarity | ||
Disulfide bondi | 219 ↔ 228 | By similarity | ||
Disulfide bondi | 235 ↔ 246 | By similarity | ||
Disulfide bondi | 240 ↔ 259 | By similarity | ||
Disulfide bondi | 261 ↔ 270 | By similarity | ||
Disulfide bondi | 277 ↔ 288 | By similarity | ||
Disulfide bondi | 282 ↔ 297 | By similarity | ||
Disulfide bondi | 299 ↔ 308 | By similarity | ||
Disulfide bondi | 315 ↔ 329 | By similarity | ||
Disulfide bondi | 323 ↔ 338 | By similarity | ||
Disulfide bondi | 340 ↔ 349 | By similarity | ||
Disulfide bondi | 356 ↔ 367 | By similarity | ||
Disulfide bondi | 361 ↔ 376 | By similarity | ||
Disulfide bondi | 378 ↔ 387 | By similarity | ||
Disulfide bondi | 393 ↔ 404 | By similarity | ||
Disulfide bondi | 398 ↔ 415 | By similarity | ||
Disulfide bondi | 417 ↔ 426 | By similarity | ||
Disulfide bondi | 433 ↔ 449 | By similarity | ||
Disulfide bondi | 443 ↔ 458 | By similarity | ||
Disulfide bondi | 460 ↔ 469 | By similarity | ||
Disulfide bondi | 476 ↔ 487 | By similarity | ||
Disulfide bondi | 481 ↔ 496 | By similarity | ||
Disulfide bondi | 498 ↔ 507 | By similarity | ||
Disulfide bondi | 514 ↔ 525 | By similarity | ||
Disulfide bondi | 519 ↔ 534 | By similarity | ||
Disulfide bondi | 536 ↔ 545 | By similarity | ||
Disulfide bondi | 552 ↔ 563 | By similarity | ||
Disulfide bondi | 557 ↔ 572 | By similarity | ||
Disulfide bondi | 574 ↔ 583 | By similarity | ||
Disulfide bondi | 590 ↔ 601 | By similarity | ||
Disulfide bondi | 595 ↔ 610 | By similarity | ||
Disulfide bondi | 612 ↔ 621 | By similarity | ||
Disulfide bondi | 626 ↔ 637 | By similarity | ||
Disulfide bondi | 631 ↔ 646 | By similarity | ||
Disulfide bondi | 648 ↔ 655 | By similarity | ||
Disulfide bondi | 662 ↔ 669 | By similarity | ||
Disulfide bondi | 664 ↔ 674 | By similarity | ||
Disulfide bondi | 676 ↔ 685 | By similarity | ||
Disulfide bondi | 692 ↔ 703 | By similarity | ||
Disulfide bondi | 697 ↔ 712 | By similarity | ||
Glycosylationi | 711 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 714 ↔ 723 | By similarity | ||
Disulfide bondi | 730 ↔ 741 | By similarity | ||
Disulfide bondi | 735 ↔ 750 | By similarity | ||
Disulfide bondi | 752 ↔ 761 | By similarity | ||
Disulfide bondi | 768 ↔ 779 | By similarity | ||
Disulfide bondi | 773 ↔ 788 | By similarity | ||
Disulfide bondi | 790 ↔ 799 | By similarity | ||
Disulfide bondi | 807 ↔ 818 | By similarity | ||
Disulfide bondi | 812 ↔ 827 | By similarity | ||
Disulfide bondi | 829 ↔ 838 | By similarity | ||
Disulfide bondi | 845 ↔ 856 | By similarity | ||
Disulfide bondi | 850 ↔ 865 | By similarity | ||
Disulfide bondi | 867 ↔ 876 | By similarity | ||
Disulfide bondi | 882 ↔ 903 | By similarity | ||
Disulfide bondi | 897 ↔ 912 | By similarity | ||
Disulfide bondi | 914 ↔ 923 | By similarity | ||
Disulfide bondi | 930 ↔ 941 | By similarity | ||
Disulfide bondi | 935 ↔ 950 | By similarity | ||
Disulfide bondi | 952 ↔ 961 | By similarity | ||
Glycosylationi | 960 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 968 ↔ 979 | By similarity | ||
Disulfide bondi | 973 ↔ 988 | By similarity | ||
Disulfide bondi | 990 ↔ 999 | By similarity | ||
Disulfide bondi | 1006 ↔ 1019 | By similarity | ||
Disulfide bondi | 1011 ↔ 1028 | By similarity | ||
Disulfide bondi | 1030 ↔ 1039 | By similarity | ||
Disulfide bondi | 1046 ↔ 1057 | By similarity | ||
Disulfide bondi | 1051 ↔ 1069 | By similarity | ||
Disulfide bondi | 1071 ↔ 1080 | By similarity | ||
Disulfide bondi | 1087 ↔ 1098 | By similarity | ||
Disulfide bondi | 1092 ↔ 1110 | By similarity | ||
Disulfide bondi | 1112 ↔ 1121 | By similarity | ||
Disulfide bondi | 1130 ↔ 1142 | By similarity | ||
Disulfide bondi | 1136 ↔ 1155 | By similarity | ||
Glycosylationi | 1139 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 1157 ↔ 1166 | By similarity | ||
Disulfide bondi | 1174 ↔ 1187 | By similarity | ||
Disulfide bondi | 1183 ↔ 1199 | By similarity | ||
Disulfide bondi | 1210 ↔ 1234 | By similarity | ||
Disulfide bondi | 1216 ↔ 1229 | By similarity | ||
Disulfide bondi | 1225 ↔ 1241 | By similarity | ||
Disulfide bondi | 1247 ↔ 1273 | By similarity | ||
Disulfide bondi | 1255 ↔ 1268 | By similarity | ||
Disulfide bondi | 1264 ↔ 1280 | By similarity |
Post-translational modificationi
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.2 Publications
Phosphorylated.
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P31695 |
PaxDbi | P31695 |
PRIDEi | P31695 |
PTM databases
GlyGeni | P31695, 3 sites |
iPTMneti | P31695 |
PhosphoSitePlusi | P31695 |
Expressioni
Tissue specificityi
Highly expressed in lung, moderately in heart kidney, and at lower levels in the ovary and skeletal muscle. A very low expression is seen in the brain, intestine, liver and testis.
Developmental stagei
Highly expressed in endothelial cells during embryonic development from 9.0 dpc.
Gene expression databases
Bgeei | ENSMUSG00000015468, Expressed in lung and 184 other tissues |
ExpressionAtlasi | P31695, baseline and differential |
Interactioni
Subunit structurei
Heterodimer of a C-terminal fragment N(TM) and a N-terminal fragment N(EC) which are probably linked by disulfide bonds.
Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4.
1 PublicationBinary interactionsi
P31695
With | #Exp. | IntAct |
---|---|---|
sel-10 [Q93794] from Caenorhabditis elegans. | 2 | EBI-643670,EBI-323098 |
GO - Molecular functioni
- Notch binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201812, 11 interactors |
CORUMi | P31695 |
IntActi | P31695, 2 interactors |
STRINGi | 10090.ENSMUSP00000015612 |
Miscellaneous databases
RNActi | P31695, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 60 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 61 – 112 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 52 | |
Domaini | 115 – 152 | EGF-like 3PROSITE-ProRule annotationAdd BLAST | 38 | |
Domaini | 153 – 189 | EGF-like 4PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 191 – 229 | EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 231 – 271 | EGF-like 6PROSITE-ProRule annotationAdd BLAST | 41 | |
Domaini | 273 – 309 | EGF-like 7; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 311 – 350 | EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 352 – 388 | EGF-like 9; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 389 – 427 | EGF-like 10PROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 429 – 470 | EGF-like 11; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 472 – 508 | EGF-like 12; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 510 – 546 | EGF-like 13; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 548 – 584 | EGF-like 14; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 586 – 622 | EGF-like 15; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 623 – 656 | EGF-like 16PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 658 – 686 | EGF-like 17PROSITE-ProRule annotationAdd BLAST | 29 | |
Domaini | 688 – 724 | EGF-like 18PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 726 – 762 | EGF-like 19PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 764 – 800 | EGF-like 20PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 803 – 839 | EGF-like 21PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 841 – 877 | EGF-like 22PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 878 – 924 | EGF-like 23PROSITE-ProRule annotationAdd BLAST | 47 | |
Domaini | 926 – 962 | EGF-like 24PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 964 – 1000 | EGF-like 25PROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 1002 – 1040 | EGF-like 26PROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 1042 – 1081 | EGF-like 27PROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 1083 – 1122 | EGF-like 28PROSITE-ProRule annotationAdd BLAST | 40 | |
Domaini | 1126 – 1167 | EGF-like 29PROSITE-ProRule annotationAdd BLAST | 42 | |
Repeati | 1166 – 1209 | LNR 1Add BLAST | 44 | |
Repeati | 1210 – 1241 | LNR 2Add BLAST | 32 | |
Repeati | 1247 – 1287 | LNR 3Add BLAST | 41 | |
Repeati | 1628 – 1657 | ANK 1Add BLAST | 30 | |
Repeati | 1661 – 1691 | ANK 2Add BLAST | 31 | |
Repeati | 1695 – 1724 | ANK 3Add BLAST | 30 | |
Repeati | 1728 – 1757 | ANK 4Add BLAST | 30 | |
Repeati | 1761 – 1790 | ANK 5Add BLAST | 30 |
Sequence similaritiesi
Belongs to the NOTCH family.Curated
Keywords - Domaini
ANK repeat, EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00940000155030 |
HOGENOMi | CLU_000576_0_0_1 |
InParanoidi | P31695 |
OrthoDBi | 7525at2759 |
PhylomeDBi | P31695 |
TreeFami | TF351641 |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR001881, EGF-like_Ca-bd_dom IPR013032, EGF-like_CS IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf IPR008297, Notch IPR035993, Notch-like_dom_sf IPR022355, Notch_4 IPR000800, Notch_dom IPR010660, Notch_NOD_dom IPR011656, Notch_NODP_dom |
Pfami | View protein in Pfam PF00023, Ank, 1 hit PF12796, Ank_2, 1 hit PF00008, EGF, 17 hits PF07645, EGF_CA, 3 hits PF12661, hEGF, 3 hits PF06816, NOD, 1 hit PF07684, NODP, 1 hit PF00066, Notch, 3 hits |
PIRSFi | PIRSF002279, Notch, 1 hit |
PRINTSi | PR01452, LNOTCHREPEAT PR01987, NOTCH4 |
SMARTi | View protein in SMART SM00248, ANK, 6 hits SM00181, EGF, 28 hits SM00179, EGF_CA, 21 hits SM00004, NL, 3 hits SM01338, NOD, 1 hit SM01339, NODP, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF57184, SSF57184, 5 hits SSF90193, SSF90193, 2 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 5 hits PS00010, ASX_HYDROXYL, 11 hits PS00022, EGF_1, 28 hits PS01186, EGF_2, 21 hits PS50026, EGF_3, 27 hits PS01187, EGF_CA, 9 hits PS50258, LNR, 3 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P31695-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQPQLLLLLL LPLNFPVILT RELLCGGSPE PCANGGTCLR LSRGQGICQC
60 70 80 90 100
APGFLGETCQ FPDPCRDTQL CKNGGSCQAL LPTPPSSRSP TSPLTPHFSC
110 120 130 140 150
TCPSGFTGDR CQTHLEELCP PSFCSNGGHC YVQASGRPQC SCEPGWTGEQ
160 170 180 190 200
CQLRDFCSAN PCANGGVCLA TYPQIQCRCP PGFEGHTCER DINECFLEPG
210 220 230 240 250
PCPQGTSCHN TLGSYQCLCP VGQEGPQCKL RKGACPPGSC LNGGTCQLVP
260 270 280 290 300
EGHSTFHLCL CPPGFTGLDC EMNPDDCVRH QCQNGATCLD GLDTYTCLCP
310 320 330 340 350
KTWKGWDCSE DIDECEARGP PRCRNGGTCQ NTAGSFHCVC VSGWGGAGCE
360 370 380 390 400
ENLDDCAAAT CAPGSTCIDR VGSFSCLCPP GRTGLLCHLE DMCLSQPCHV
410 420 430 440 450
NAQCSTNPLT GSTLCICQPG YSGSTCHQDL DECQMAQQGP SPCEHGGSCI
460 470 480 490 500
NTPGSFNCLC LPGYTGSRCE ADHNECLSQP CHPGSTCLDL LATFHCLCPP
510 520 530 540 550
GLEGRLCEVE VNECTSNPCL NQAACHDLLN GFQCLCLPGF TGARCEKDMD
560 570 580 590 600
ECSSTPCANG GRCRDQPGAF YCECLPGFEG PHCEKEVDEC LSDPCPVGAS
610 620 630 640 650
CLDLPGAFFC LCRPGFTGQL CEVPLCTPNM CQPGQQCQGQ EHRAPCLCPD
660 670 680 690 700
GSPGCVPAED NCPCHHGHCQ RSLCVCDEGW TGPECETELG GCISTPCAHG
710 720 730 740 750
GTCHPQPSGY NCTCPAGYMG LTCSEEVTAC HSGPCLNGGS CSIRPEGYSC
760 770 780 790 800
TCLPSHTGRH CQTAVDHCVS ASCLNGGTCV NKPGTFFCLC ATGFQGLHCE
810 820 830 840 850
EKTNPSCADS PCRNKATCQD TPRGARCLCS PGYTGSSCQT LIDLCARKPC
860 870 880 890 900
PHTARCLQSG PSFQCLCLQG WTGALCDFPL SCQKAAMSQG IEISGLCQNG
910 920 930 940 950
GLCIDTGSSY FCRCPPGFQG KLCQDNVNPC EPNPCHHGST CVPQPSGYVC
960 970 980 990 1000
QCAPGYEGQN CSKVLDACQS QPCHNHGTCT SRPGGFHCAC PPGFVGLRCE
1010 1020 1030 1040 1050
GDVDECLDRP CHPSGTAACH SLANAFYCQC LPGHTGQRCE VEMDLCQSQP
1060 1070 1080 1090 1100
CSNGGSCEIT TGPPPGFTCH CPKGFEGPTC SHKALSCGIH HCHNGGLCLP
1110 1120 1130 1140 1150
SPKPGSPPLC ACLSGFGGPD CLTPPAPPGC GPPSPCLHNG TCTETPGLGN
1160 1170 1180 1190 1200
PGFQCTCPPD SPGPRCQRPG ASGCEGRGGD GTCDAGCSGP GGDWDGGDCS
1210 1220 1230 1240 1250
LGVPDPWKGC PPHSQCWLLF RDGRCHPQCD SEECLFDGYD CEIPLTCIPA
1260 1270 1280 1290 1300
YDQYCRDHFH NGHCEKGCNN AECGWDGGDC RPEGEDSEGR PSLALLVVLR
1310 1320 1330 1340 1350
PPALDQQLLA LARVLSLTLR VGLWVRKDSE GRNMVFPYPG TRAKEELSGA
1360 1370 1380 1390 1400
RDSSSWERQA PPTQPLGKET ESLGAGFVVV MGVDLSRCGP EHPASRCPWD
1410 1420 1430 1440 1450
SGLLLRFLAA MAAVGALEPL LPGPLLAAHP QAGTRPSANQ LPWPILCSPV
1460 1470 1480 1490 1500
VGVLLLALGA LLVLQLIRRR RREHGALWLP PGFIRRPQTQ QAPHRRRPPL
1510 1520 1530 1540 1550
GEDNIGLKAL KPEAEVDEDG VAMCSGPEEG EAEETASASR CQLWPLNSGC
1560 1570 1580 1590 1600
GELPQAAMLT PPQECESEVL DVDTCGPDGV TPLMSAVFCG GVQSTTGASP
1610 1620 1630 1640 1650
QRLGLGNLEP WEPLLDRGAC PQAHTVGTGE TPLHLAARFS RPTAARRLLE
1660 1670 1680 1690 1700
AGANPNQPDR AGRTPLHTAV AADAREVCQL LLASRQTTVD ARTEDGTTPL
1710 1720 1730 1740 1750
MLAARLAVED LVEELIAARA DVGARDKRGK TALHWAAAVN NARAARSLLQ
1760 1770 1780 1790 1800
AGADKDAQDS REQTPLFLAA REGAVEVAQL LLELGAARGL RDQAGLAPGD
1810 1820 1830 1840 1850
VARQRSHWDL LTLLEGAGPT TQEARAHART TPGGGAAPRC RTLSAGARPR
1860 1870 1880 1890 1900
GGGACLQART WSVDLGARGG KVYARCRSRS GSCGGPTTRG RRFSAGSRGR
1910 1920 1930 1940 1950
RGARASQDDW PRDWVALEAC GSACSAPIPP PSLTPSPERG SPQVAWGLPV
1960
HQEIPLNSVV RNLN
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UX69 | G3UX69_MOUSE | Neurogenic locus notch homolog prot... | Notch4 | 978 | Annotation score: | ||
G3UZH3 | G3UZH3_MOUSE | Neurogenic locus notch homolog prot... | Notch4 | 43 | Annotation score: |
Sequence cautioni
The sequence BAA32281 differs from that shown. Contaminating sequence. Sequence of unknown origin.Curated
The sequence BAA32283 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAA32284 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAA32285 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 43 | R → Q in AAB38377 (PubMed:1312643).Curated | 1 | |
Sequence conflicti | 298 | L → P in AAC52630 (PubMed:8681805).Curated | 1 | |
Sequence conflicti | 884 | K → M in AAB38377 (PubMed:1312643).Curated | 1 | |
Sequence conflicti | 927 | V → M in AAB38377 (PubMed:1312643).Curated | 1 | |
Sequence conflicti | 966 | D → E in AAB38377 (PubMed:1312643).Curated | 1 | |
Sequence conflicti | 1245 | L → P in AAC52630 (PubMed:8681805).Curated | 1 | |
Sequence conflicti | 1437 | S → P in AAC52631 (PubMed:8681805).Curated | 1 | |
Sequence conflicti | 1437 | S → P in AAC52630 (PubMed:8681805).Curated | 1 | |
Sequence conflicti | 1437 | S → P in AAB38377 (PubMed:1312643).Curated | 1 | |
Sequence conflicti | 1469 | R → Q in BAA32283 (PubMed:10233982).Curated | 1 | |
Sequence conflicti | 1469 | R → Q in BAA32284 (PubMed:10233982).Curated | 1 | |
Sequence conflicti | 1489 | T → A in AAB38377 (PubMed:1312643).Curated | 1 | |
Sequence conflicti | 1549 | G → S in AAB38377 (PubMed:1312643).Curated | 1 | |
Sequence conflicti | 1688 | T → S in AAB38377 (PubMed:1312643).Curated | 1 | |
Sequence conflicti | 1836 | A → S in AAC52630 (PubMed:8681805).Curated | 1 | |
Sequence conflicti | 1836 | A → S in AAC52631 (PubMed:8681805).Curated | 1 | |
Sequence conflicti | 1838 | P → A in AAB38377 (PubMed:1312643).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80456 mRNA Translation: AAB38377.1 U43691 mRNA Translation: AAC52630.1 U43691 mRNA Translation: AAC52631.1 AF030001 Genomic DNA Translation: AAB82004.1 CT009767 Genomic DNA Translation: CAM18615.1 AB016771 Genomic DNA Translation: BAA32281.1 Sequence problems. AB016772 mRNA Translation: BAA32283.1 Different initiation. AB016773 mRNA Translation: BAA32284.1 Different initiation. AB016774 mRNA Translation: BAA32285.1 Different initiation. |
CCDSi | CCDS28647.1 |
PIRi | A38072, TVMVT3 T09059 |
RefSeqi | NP_035059.2, NM_010929.2 |
Genome annotation databases
Ensembli | ENSMUST00000015612; ENSMUSP00000015612; ENSMUSG00000015468 |
GeneIDi | 18132 |
KEGGi | mmu:18132 |
UCSCi | uc008ccs.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80456 mRNA Translation: AAB38377.1 U43691 mRNA Translation: AAC52630.1 U43691 mRNA Translation: AAC52631.1 AF030001 Genomic DNA Translation: AAB82004.1 CT009767 Genomic DNA Translation: CAM18615.1 AB016771 Genomic DNA Translation: BAA32281.1 Sequence problems. AB016772 mRNA Translation: BAA32283.1 Different initiation. AB016773 mRNA Translation: BAA32284.1 Different initiation. AB016774 mRNA Translation: BAA32285.1 Different initiation. |
CCDSi | CCDS28647.1 |
PIRi | A38072, TVMVT3 T09059 |
RefSeqi | NP_035059.2, NM_010929.2 |
3D structure databases
SMRi | P31695 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201812, 11 interactors |
CORUMi | P31695 |
IntActi | P31695, 2 interactors |
STRINGi | 10090.ENSMUSP00000015612 |
PTM databases
GlyGeni | P31695, 3 sites |
iPTMneti | P31695 |
PhosphoSitePlusi | P31695 |
Proteomic databases
MaxQBi | P31695 |
PaxDbi | P31695 |
PRIDEi | P31695 |
Genome annotation databases
Ensembli | ENSMUST00000015612; ENSMUSP00000015612; ENSMUSG00000015468 |
GeneIDi | 18132 |
KEGGi | mmu:18132 |
UCSCi | uc008ccs.1, mouse |
Organism-specific databases
CTDi | 4855 |
MGIi | MGI:107471, Notch4 |
Phylogenomic databases
eggNOGi | KOG1217, Eukaryota |
GeneTreei | ENSGT00940000155030 |
HOGENOMi | CLU_000576_0_0_1 |
InParanoidi | P31695 |
OrthoDBi | 7525at2759 |
PhylomeDBi | P31695 |
TreeFami | TF351641 |
Enzyme and pathway databases
Reactomei | R-MMU-1912420, Pre-NOTCH Processing in Golgi R-MMU-350054, Notch-HLH transcription pathway R-MMU-9013700, NOTCH4 Activation and Transmission of Signal to the Nucleus R-MMU-9604323, Negative regulation of NOTCH4 signaling |
Miscellaneous databases
BioGRID-ORCSi | 18132, 3 hits in 17 CRISPR screens |
ChiTaRSi | Notch4, mouse |
PROi | PR:P31695 |
RNActi | P31695, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015468, Expressed in lung and 184 other tissues |
ExpressionAtlasi | P31695, baseline and differential |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR001881, EGF-like_Ca-bd_dom IPR013032, EGF-like_CS IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR009030, Growth_fac_rcpt_cys_sf IPR008297, Notch IPR035993, Notch-like_dom_sf IPR022355, Notch_4 IPR000800, Notch_dom IPR010660, Notch_NOD_dom IPR011656, Notch_NODP_dom |
Pfami | View protein in Pfam PF00023, Ank, 1 hit PF12796, Ank_2, 1 hit PF00008, EGF, 17 hits PF07645, EGF_CA, 3 hits PF12661, hEGF, 3 hits PF06816, NOD, 1 hit PF07684, NODP, 1 hit PF00066, Notch, 3 hits |
PIRSFi | PIRSF002279, Notch, 1 hit |
PRINTSi | PR01452, LNOTCHREPEAT PR01987, NOTCH4 |
SMARTi | View protein in SMART SM00248, ANK, 6 hits SM00181, EGF, 28 hits SM00179, EGF_CA, 21 hits SM00004, NL, 3 hits SM01338, NOD, 1 hit SM01339, NODP, 1 hit |
SUPFAMi | SSF48403, SSF48403, 1 hit SSF57184, SSF57184, 5 hits SSF90193, SSF90193, 2 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 5 hits PS00010, ASX_HYDROXYL, 11 hits PS00022, EGF_1, 28 hits PS01186, EGF_2, 21 hits PS50026, EGF_3, 27 hits PS01187, EGF_CA, 9 hits PS50258, LNR, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NOTC4_MOUSE | |
Accessioni | P31695Primary (citable) accession number: P31695 Secondary accession number(s): A2CG28 Q9R1X0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | April 10, 2019 | |
Last modified: | December 2, 2020 | |
This is version 199 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families