djlA

Functionⁱ

Regulatory DnaK co-chaperone. Direct interaction between DnaK and DjlA is needed for the induction of the wcaABCDE operon, involved in the synthesis of a colanic acid polysaccharide capsule, possibly through activation of the RcsB/RcsC phosphotransfer signaling pathway. The colanic acid capsule may help the bacterium survive conditions outside the host.UniRule annotation3 Publications

GO - Molecular functionⁱ

chaperone binding
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 11544239

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chaperone

Molecular function

Chaperone

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11570-MONOMER

BioCycⁱ

EcoCyc:EG11570-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Co-chaperone protein DjlAUniRule annotationCurated Alternative name(s): DnaJ-like protein DjlACurated
Gene namesⁱ	Name:djlA1 PublicationUniRule annotation Synonyms:yabH Ordered Locus Names:b0055, JW0054
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11570 djlA

EcoGeneⁱ

EG11570 djlA

Subcellular locationⁱ

Cell inner membrane
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01153
3 Publications
Manual assertion based on experiment inⁱ
- Ref.5
  "A novel DnaJ-like protein in Escherichia coli inserts into the cytoplasmic membrane with a type III topology."
  Clarke D.J., Jacq A., Holland I.B.
  Mol. Microbiol. 20:1273-1286(1996) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45, SUBCELLULAR LOCATION, TOPOLOGY.
- Ref.6
  "Positive control of the two-component RcsC/B signal transduction network by DjIA: a member of the DnaJ family of molecular chaperones in Escherichia coli."
  Kelley W.L., Georgopoulos C.
  Mol. Microbiol. 25:913-931(1997) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF HIS-233.
- Ref.8
  "DjlA is a third DnaK co-chaperone of Escherichia coli, and DjlA-mediated induction of colanic acid capsule requires DjlA-DnaK interaction."
  Genevaux P., Wawrzynow A., Zylicz M., Georgopoulos C., Kelley W.L.
  J. Biol. Chem. 276:7906-7912(2001) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION.
; Single-pass type III membrane protein
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_01153
3 Publications
Manual assertion based on experiment inⁱ
- Ref.5
  "A novel DnaJ-like protein in Escherichia coli inserts into the cytoplasmic membrane with a type III topology."
  Clarke D.J., Jacq A., Holland I.B.
  Mol. Microbiol. 20:1273-1286(1996) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-45, SUBCELLULAR LOCATION, TOPOLOGY.
- Ref.6
  "Positive control of the two-component RcsC/B signal transduction network by DjIA: a member of the DnaJ family of molecular chaperones in Escherichia coli."
  Kelley W.L., Georgopoulos C.
  Mol. Microbiol. 25:913-931(1997) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF HIS-233.
- Ref.8
  "DjlA is a third DnaK co-chaperone of Escherichia coli, and DjlA-mediated induction of colanic acid capsule requires DjlA-DnaK interaction."
  Genevaux P., Wawrzynow A., Zylicz M., Georgopoulos C., Kelley W.L.
  J. Biol. Chem. 276:7906-7912(2001) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION.

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 6	PeriplasmicUniRule annotation2 Publications	6
Transmembraneⁱ	7 – 31	HelicalUniRule annotationAdd BLAST	25
Topological domainⁱ	32 – 271	CytoplasmicUniRule annotation2 PublicationsAdd BLAST	240

GO - Cellular componentⁱ

integral component of membrane Source: UniProtKB-KW
membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 9364917
plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 17309111

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	15	L → R: Loss of activation of rcs. 1 Publication	1
Mutagenesisⁱ	16	M → R: Only partial activation of rcs. 1 Publication	1
Mutagenesisⁱ	233	H → Q: Loss of activity. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000209424	1 – 271	Co-chaperone protein DjlAAdd BLAST		271

Proteomic databases

PaxDbⁱ	P31680
PRIDEⁱ	P31680

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation1 Publication

GO - Molecular functionⁱ

chaperone binding
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 11544239

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4261616, 19 interactors
IntActⁱ	P31680, 7 interactors
STRINGⁱ	316385.ECDH10B_0056

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P31680
SMRⁱ	P31680
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	205 – 271	JUniRule annotationAdd BLAST		67

Domainⁱ

The transmembrane domain is a dimerization domain.UniRule annotation1 Publication

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG41066UG Bacteria COG1076 LUCA
HOGENOMⁱ	HOG000272779
InParanoidⁱ	P31680
KEGG Orthology (KO) More...KOⁱ	K05801

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06257 DnaJ, 1 hit
Gene3Dⁱ	1.10.287.110, 1 hit 1.10.3680.10, 1 hit
HAMAPⁱ	MF_01153 DjlA, 1 hit
InterProⁱ	View protein in InterPro IPR023749 DjlA IPR007791 DjlA_N IPR001623 DnaJ_domain IPR036869 J_dom_sf IPR029024 TerB-like
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00226 DnaJ, 1 hit PF05099 TerB, 1 hit
PRINTSⁱ	PR00625 JDOMAIN
SMARTⁱ	View protein in SMART SM00271 DnaJ, 1 hit
SUPFAMⁱ	SSF46565 SSF46565, 1 hit
PROSITEⁱ	View protein in PROSITE PS50076 DNAJ_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P31680-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MQYWGKIIGV AVALLMGGGF WGVVLGLLIG HMFDKARSRK MAWFANQRER 
        60         70         80         90        100
QALFFATTFE VMGHLTKSKG RVTEADIHIA SQLMDRMNLH GASRTAAQNA 
       110        120        130        140        150
FRVGKSDNYP LREKMRQFRS VCFGRFDLIR MFLEIQIQAA FADGSLHPNE 
       160        170        180        190        200
RAVLYVIAEE LGISRAQFDQ FLRMMQGGAQ FGGGYQQQTG GGNWQQAQRG 
       210        220        230        240        250
PTLEDACNVL GVKPTDDATT IKRAYRKLMS EHHPDKLVAK GLPPEMMEMA 
       260        270 
KQKAQEIQQA YELIKQQKGF K

Length:271

Mass (Da):30,579

Last modified:July 1, 1993 - v1

Checksum:ⁱ80A0FC28F6D470DF

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	195	Q → L in AAT42473 (PubMed:12664169).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC73166.1 AP009048 Genomic DNA Translation: BAB96623.1 AY625119 Genomic DNA Translation: AAT42473.1
PIRⁱ	G64726
NCBI Reference Sequences More...RefSeqⁱ	NP_414597.1, NC_000913.3 WP_001200579.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73166; AAC73166; b0055 BAB96623; BAB96623; BAB96623
GeneIDⁱ	948992
KEGGⁱ	ecj:JW0054 eco:b0055
PATRICⁱ	fig\|1411691.4.peg.2228

Protein	Similar proteins		Species	Score	Length	Source
P31680	Dna-J like membrane chaperone protein		ECOLX		278	UniRef100_P31680
Co-chaperone protein DjlA		Escherichia coli H386		278
Co-chaperone protein DjlA		Escherichia coli H591		278
Co-chaperone protein DjlA		Escherichia coli M718		278
Co-chaperone protein DjlA		Escherichia coli H420		278
+43

Protein	Similar proteins		Species	Score	Length	Source
P31680	Co-chaperone protein DjlA		ECOL6		271	UniRef90_P31680
Co-chaperone protein DjlA		ECO57		271
Co-chaperone protein DjlA		SHIFL		271
Dna-J like membrane chaperone protein		ECOLX		278
Co-chaperone protein DjlA		Escherichia coli H386		278
+596

Protein	Similar proteins		Species	Score	Length	Source
P31680	Co-chaperone protein DjlA		ECOL6		271	UniRef50_P31680
Co-chaperone protein DjlA		ECO57		271
Co-chaperone protein DjlA		SHIFL		271
Co-chaperone protein DjlA		PHOPR		277
Co-chaperone protein DjlA		PHOLL		273
+1342

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC73166.1 AP009048 Genomic DNA Translation: BAB96623.1 AY625119 Genomic DNA Translation: AAT42473.1
PIRⁱ	G64726
RefSeqⁱ	NP_414597.1, NC_000913.3 WP_001200579.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P31680
SMRⁱ	P31680
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261616, 19 interactors
IntActⁱ	P31680, 7 interactors
STRINGⁱ	316385.ECDH10B_0056

Proteomic databases

PaxDbⁱ	P31680
PRIDEⁱ	P31680

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73166; AAC73166; b0055 BAB96623; BAB96623; BAB96623
GeneIDⁱ	948992
KEGGⁱ	ecj:JW0054 eco:b0055
PATRICⁱ	fig\|1411691.4.peg.2228

Organism-specific databases

EchoBASEⁱ	EB1530
EcoGeneⁱ	EG11570 djlA

Phylogenomic databases

eggNOGⁱ	ENOG41066UG Bacteria COG1076 LUCA
HOGENOMⁱ	HOG000272779
InParanoidⁱ	P31680
KOⁱ	K05801

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11570-MONOMER

BioCycⁱ

EcoCyc:EG11570-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P31680

PROⁱ

PR:P31680

Family and domain databases

CDDⁱ	cd06257 DnaJ, 1 hit
Gene3Dⁱ	1.10.287.110, 1 hit 1.10.3680.10, 1 hit
HAMAPⁱ	MF_01153 DjlA, 1 hit
InterProⁱ	View protein in InterPro IPR023749 DjlA IPR007791 DjlA_N IPR001623 DnaJ_domain IPR036869 J_dom_sf IPR029024 TerB-like
Pfamⁱ	View protein in Pfam PF00226 DnaJ, 1 hit PF05099 TerB, 1 hit
PRINTSⁱ	PR00625 JDOMAIN
SMARTⁱ	View protein in SMART SM00271 DnaJ, 1 hit
SUPFAMⁱ	SSF46565 SSF46565, 1 hit
PROSITEⁱ	View protein in PROSITE PS50076 DNAJ_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DJLA_ECOLI
Accessionⁱ	P31680Primary (citable) accession number: P31680 Secondary accession number(s): Q6IU30
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
	Last sequence update:	July 1, 1993
	Last modified:	November 7, 2018
	This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P31680 (DJLA_ECOLI)

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Co-chaperone protein DjlA

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ