UniProtKB - P31667 (RPNA_ECOLI)
Recombination-promoting nuclease RpnA
rpnA
Functioni
A low activity DNA endonuclease yielding 3'-hydroxyl ends, equally active on ss or dsDNA, not active on dsRNA (PubMed:28096446).
Shows no sequence specificity (PubMed:28096446).
Upon expression enhances RecA-independent DNA recombination 49-fold, concomitantly reducing viability by 88% and probably inducing DNA damage as measured by induction of the SOS repair response in RecA cells (PubMed:28096446).
RecA-independent DNA recombination leads to replacement of recipient genes with large segments of donor DNA rather than DNA addition to the donor strain; increased expression of RpnA leads to smaller replacement segments, suggesting this protein may play a role in generating crossover events (PubMed:28096446).
1 PublicationCofactori
Activity regulationi
pH dependencei
GO - Molecular functioni
- double-stranded DNA endodeoxyribonuclease activity Source: EcoCyc
- endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: UniProtKB
GO - Biological processi
- DNA recombination Source: UniProtKB
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | DNA recombination |
Ligand | Magnesium |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11750-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rpnA1 Publication Synonyms:yhgA Ordered Locus Names:b3411, JW3374 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 11 | D → A: No longer increases recombination efficiency, toxicity or SOS response. 1 Publication | 1 | |
Mutagenesisi | 63 | D → A: No longer increases recombination efficiency, toxicity or SOS response. No nuclease activity. 1 Publication | 1 | |
Mutagenesisi | 82 | E → A: No longer increases recombination efficiency, toxicity or SOS response. 1 Publication | 1 | |
Mutagenesisi | 84 | Q → A: No longer increases recombination efficiency or SOS response, more toxic than wild-type. 1 Publication | 1 | |
Mutagenesisi | 84 | Q → K: No longer increases recombination efficiency, toxicity or SOS response. 1 Publication | 1 | |
Mutagenesisi | 94 | R → A: No longer increases recombination efficiency or SOS response, more toxic than wild-type. 1 Publication | 1 | |
Mutagenesisi | 165 | D → A: Hyperactive mutant, increases recombination efficiency, more toxic than wild-type. Increased nuclease activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169544 | 1 – 292 | Recombination-promoting nuclease RpnAAdd BLAST | 292 |
Proteomic databases
PaxDbi | P31667 |
PRIDEi | P31667 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263493, 9 interactors |
DIPi | DIP-12335N |
IntActi | P31667, 1 interactor |
STRINGi | 511145.b3411 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG5464, Bacteria |
HOGENOMi | CLU_059548_1_0_6 |
InParanoidi | P31667 |
PhylomeDBi | P31667 |
Family and domain databases
InterProi | View protein in InterPro IPR010106, RpnA IPR006842, Transposase_31 |
Pfami | View protein in Pfam PF04754, Transposase_31, 1 hit |
TIGRFAMsi | TIGR01784, T_den_put_tspse, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSKKQSSTPH DALFKLFLRQ PDTARDFLAF HLPAPIHALC DMKTLKLESS
60 70 80 90 100
SFIDDDLRES YSDVLWSVKT EQGPGYIYCL IEHQSTSNKL IAFRMMRYAI
110 120 130 140 150
AAMQNHLDAG YKTLPMVVPL LFYHGIESPY PYSLCWLDCF ADPKLARQLY
160 170 180 190 200
ASAFPLIDVT VMPDDEIMQH RRMALLELIQ KHIRQRDLMG LVEQMACLLS
210 220 230 240 250
SGYANDRQIK GLFNYILQTG DAVRFNDFID GVAERSPKHK ESLMTIAERL
260 270 280 290
RQEGEQSKAL HIAKIMLESG VPLADIMRFT GLSEEELAAA SQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15587 Genomic DNA No translation available. U18997 Genomic DNA Translation: AAA58209.1 U00096 Genomic DNA Translation: AAC76436.1 AP009048 Genomic DNA Translation: BAE77880.1 |
PIRi | F65136 |
RefSeqi | NP_417870.1, NC_000913.3 WP_000039062.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76436; AAC76436; b3411 BAE77880; BAE77880; BAE77880 |
GeneIDi | 947917 |
KEGGi | ecj:JW3374 eco:b3411 |
PATRICi | fig|1411691.4.peg.3318 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15587 Genomic DNA No translation available. U18997 Genomic DNA Translation: AAA58209.1 U00096 Genomic DNA Translation: AAC76436.1 AP009048 Genomic DNA Translation: BAE77880.1 |
PIRi | F65136 |
RefSeqi | NP_417870.1, NC_000913.3 WP_000039062.1, NZ_SSZK01000008.1 |
3D structure databases
SMRi | P31667 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263493, 9 interactors |
DIPi | DIP-12335N |
IntActi | P31667, 1 interactor |
STRINGi | 511145.b3411 |
Proteomic databases
PaxDbi | P31667 |
PRIDEi | P31667 |
Genome annotation databases
EnsemblBacteriai | AAC76436; AAC76436; b3411 BAE77880; BAE77880; BAE77880 |
GeneIDi | 947917 |
KEGGi | ecj:JW3374 eco:b3411 |
PATRICi | fig|1411691.4.peg.3318 |
Organism-specific databases
EchoBASEi | EB1700 |
Phylogenomic databases
eggNOGi | COG5464, Bacteria |
HOGENOMi | CLU_059548_1_0_6 |
InParanoidi | P31667 |
PhylomeDBi | P31667 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11750-MONOMER |
Miscellaneous databases
PROi | PR:P31667 |
Family and domain databases
InterProi | View protein in InterPro IPR010106, RpnA IPR006842, Transposase_31 |
Pfami | View protein in Pfam PF04754, Transposase_31, 1 hit |
TIGRFAMsi | TIGR01784, T_den_put_tspse, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RPNA_ECOLI | |
Accessioni | P31667Primary (citable) accession number: P31667 Secondary accession number(s): Q2M776 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families