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Entry version 93 (07 Oct 2020)
Sequence version 1 (01 Jul 1993)
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Protein

RNA2 polyprotein

Gene
N/A
Organism
Cowpea severe mosaic virus (strain DG) (CPSMV)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Responsible for viral RNA2 accumulation. May function by recruiting the RNA1-encoded polyprotein that contains the replication protein to RNA2 and enable its replication.By similarity
Transports the viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. Binds to GTP and to single-stranded RNA and single-stranded DNA in a non-sequence-specific manner.By similarity
Together with the mature small capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the small capsid protein. The large capsid protein interacts with the viral RNA.By similarity
Together with the large capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the small capsid protein. The mature small capsid protein forms the turrets at the fivefold axes of the viral particle.By similarity
The cleavable C-terminus of small capsid protein seems to be involved in viral assembly and RNA packaging. After virus assembly, these amino acids are cleaved off during the normal maturation of the virus. Also seems to act as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs.By similarity
Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs.By similarity

Caution

It is uncertain whether Met-1 or Met-93 is the initiator.Curated

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, RNA-binding, Suppressor of RNA silencing, Viral movement protein
Biological processTransport
LigandGTP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
RNA2 polyprotein
Alternative name(s):
Genome polyprotein M
M RNA polyprotein
Middle component RNA polyprotein
P2
Cleaved into the following 6 chains:
Alternative name(s):
P58
Movement protein
Short name:
MP
Large capsid protein
Short name:
LCP
Alternative name(s):
Coat protein VP37
L subunit
Large coat protein
Alternative name(s):
S subunit
Alternative name(s):
Coat protein VP23
Small capsid protein, N-terminus part
Small coat protein, N-terminus part
Alternative name(s):
Small coat protein C-terminus part
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiCowpea severe mosaic virus (strain DG) (CPSMV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri31716 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaOrthornaviraePisuviricotaPisoniviricetesPicornaviralesSecoviridaeComovirinaeComovirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiCalopogonium mucunoides [TaxID: 132433]
Canavalia ensiformis (Jack bean) (Dolichos ensiformis) [TaxID: 3823]
Centrosema pubescens [TaxID: 185703]
Crotalaria juncea (Sunn hemp) [TaxID: 3829]
Desmodium [TaxID: 53866]
Glycine max (Soybean) (Glycine hispida) [TaxID: 3847]
Macroptilium lathyroides [TaxID: 260885]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Psophocarpus tetragonolobus (Winged bean) (Dolichos tetragonolobus) [TaxID: 3891]
Vigna radiata (Mung bean) [TaxID: 157791]
Vigna unguiculata (Cowpea) [TaxID: 3917]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000008566 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Genome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cell junction, T=3 icosahedral capsid protein, Virion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004458421 – 1002RNA2 polyproteinAdd BLAST1002
ChainiPRO_00004458431 – 433VP58Add BLAST433
ChainiPRO_000003702893 – 433Movement proteinAdd BLAST341
ChainiPRO_0000037029434 – 807Large capsid proteinAdd BLAST374
ChainiPRO_0000445844808 – 1002Small capsid protein precursorAdd BLAST195
ChainiPRO_0000037030808 – 985Mature small capsid proteinAdd BLAST178
ChainiPRO_0000445845986 – 1002Small capsid protein C-terminus partAdd BLAST17

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins.By similarity
The C-terminal amino acids of the small capsid protein is specifically cleaved by the RNA1 encoded picornain 3C-like protease during maturation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei433 – 434Cleavage; by viral protease1 Publication2
Sitei806 – 807Cleavage; by viral proteaseBy similarity2
Sitei985 – 986CleavageBy similarity2

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with the large capsid protein.

By similarity

Interacts with the small capsid protein. Homomultimer; assembles as pentons.

Interacts with the movement protein (via C-terminus).

By similarity

Interacts (via C-terminus) with the large capsid protein.

By similarity

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P31630

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni46 – 68HydrophobicSequence analysisAdd BLAST23
Regioni392 – 398Involved in tubule formation by the movement proteinBy similarity7

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The C-terminus is required for efficient assembly and RNA packaging.By similarity
Contains a beta-sheet structure called beta-barrel jelly roll.By similarity
Contains a beta-sheet structure called beta-barrel jelly roll.By similarity
The C-terminus is involved in binding to the large capsid protein, and hence to the virion.By similarity

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.120.20, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003181, Como_LCP
IPR003182, RNA2_polyprotein
IPR029053, Viral_coat

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02247, Como_LCP, 1 hit
PF02248, Como_SCP, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative initiation. AlignAdd to basket
Isoform 1 (identifier: P31630-1) [UniParc]FASTAAdd to basket
Also known as: RNA2 polyprotein

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSTFRYKCKQ LDQEIQWWFS GTGNRAFWKF EKKLAELHEW YWSLALDPFP
60 70 80 90 100
YSGFFYKCFY ELFQLWVKLG LIVQVSYLIL LLDFFVYTIP KKMASQIETT
110 120 130 140 150
VEKVKQSGIP ADILRKRAVD YWKKNNSHNS QMQDVLPNVD EIYEGMRANI
160 170 180 190 200
AKYLGRSSTV TSIAKLGKCK VYRKKNIPLA NLPSLQTSCV PITLTEESVG
210 220 230 240 250
NSDYTTEETN SEVKSLHVGA IEIVMNSFAS SDCNILGGFL LIDTCHTDIN
260 270 280 290 300
NAIRSIFVAP MRGGRPIRMI SFPDTLVQIE PNMNKRFQLL CTTSNGDFMQ
310 320 330 340 350
GRDLAMMHVN VLAHAVTHTS TYTPTPYYEK ILSREKGFIV EYLNRMTYAV
360 370 380 390 400
HNQNHPTEKD LLESDFQFDF EGQPVLKRIS STKAIFSKGS SFRYMISGKK
410 420 430 440 450
EHKIDKPRLE EDGSKSYIDG LQDTFDTTHA TLQSGADLFK RNLDDVSTIS
460 470 480 490 500
DTMLGAMIGQ TKVVIPKTLV AGTVLKSGPL SDVMQQGSFR STIALQRTHI
510 520 530 540 550
ITGKIHVVAM LETAVNTGLG LAICFNSGIR GKASADIYAT CSQDAMIWNP
560 570 580 590 600
SCTKVMQYAF NPNPCSDGWS LAFLERTGYH CVVTCVTGWT GTPLQDTFMT
610 620 630 640 650
INWHISREAC VPKIYTIFDP EPDMMLNRWM GRAIFPQQST QVVRRMPLSI
660 670 680 690 700
GGGAGAKNSI LMNLPNAILS MWRYFKADLE FELIKMSSPY INATIAFFVA
710 720 730 740 750
FGDLSDDTVN FEAFPHKLIV FSDKQDRTTI SFSKDEFLMA WSTQVRPDTK
760 770 780 790 800
LSEDGCPYLY AITHNGVSSS VEGDFILGIK MVGLKAVENI GVNPGIIGSR
810 820 830 840 850
LLGAVAQSGQ TQQVWNKIWR IGTPPQATDG LFSFSIDLLG VELVTDGQEG
860 870 880 890 900
AVSVLSSSPV ANLLRTAAWK CGTLHVKVVM TGRVTTTRAN WASHTQMSLV
910 920 930 940 950
NSDNAQHYEA QKWSVSTPHA WEKEFSIDIC GPNRGFEMWR SSWSNQTTWI
960 970 980 990 1000
LEFTVAGASQ SAIFEIFYRL DNSWKSAGNV LMPPLLVGNP RLDIKGRAAA

AA
Length:1,002
Mass (Da):112,109
Last modified:July 1, 1993 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9C27751AD5BD7D39
GO
Isoform 2 (identifier: P31630-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.

Show »
Length:910
Mass (Da):100,752
Checksum:i5788CF5B68D75D35
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0599791 – 92Missing in isoform 2. Add BLAST92

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M83309 Genomic RNA No translation available.

Protein sequence database of the Protein Information Resource

More...
PIRi
A42454, GNWXCS

Keywords - Coding sequence diversityi

Alternative initiation

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83309 Genomic RNA No translation available.
PIRiA42454, GNWXCS

3D structure databases

SMRiP31630
ModBaseiSearch...

Family and domain databases

Gene3Di2.60.120.20, 2 hits
InterProiView protein in InterPro
IPR003181, Como_LCP
IPR003182, RNA2_polyprotein
IPR029053, Viral_coat
PfamiView protein in Pfam
PF02247, Como_LCP, 1 hit
PF02248, Como_SCP, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPOL2_CPSMV
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P31630
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 7, 2020
This is version 93 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
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