The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P31570 (BTUR_SALTY)
Protein
Corrinoid adenosyltransferase CobA
Gene
btuR
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Functioni
Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids.
Catalytic activityi
- 2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)yrinate a,c-diamide + 3 H+ + oxidized [electron-transfer flavoprotein] + 2 triphosphateEC:2.5.1.17
- 2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)alamin + 3 H+ + oxidized [electron-transfer flavoprotein] + 2 triphosphateEC:2.5.1.17
: adenosylcobalamin biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide. This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 42 | ATPBy similarity | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cob(I)yrinic acid a,c-diamide adenosyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- cobalamin biosynthetic process Source: UniProtKB-UniPathway
- porphyrin-containing compound biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Cobalamin biosynthesis, Porphyrin biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.5.1.17, 2169 |
UniPathwayi | UPA00148;UER00233 |
Names & Taxonomyi
Protein namesi | Recommended name: Corrinoid adenosyltransferase CobA (EC:2.5.1.17)Alternative name(s): Cob(II)alamin adenosyltransferase Cob(II)yrinic acid a,c-diamide adenosyltransferase Cobinamide/cobalamin adenosyltransferase |
Gene namesi | Name:btuR Synonyms:cobA1 Publication Ordered Locus Names:STM1718 |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mild growth defect on 1,2-propanediol-cyanocobalamin medium; no growth is seen in a double cobA-pduO deletion.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000065010 | 1 – 196 | Corrinoid adenosyltransferase CobAAdd BLAST | 196 |
Proteomic databases
PaxDbi | P31570 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P31570 |
SMRi | P31570 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P31570 |
Family & Domainsi
Sequence similaritiesi
Belongs to the Cob(I)alamin adenosyltransferase family.Curated
Phylogenomic databases
HOGENOMi | CLU_088595_0_0_6 |
OMAi | HAMGEGF |
PhylomeDBi | P31570 |
Family and domain databases
CDDi | cd00561, CobA_CobO_BtuR, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003724, CblAdoTrfase_CobA IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02572, CobA_CobO_BtuR, 1 hit |
PIRSFi | PIRSF015617, Adensltrnsf_CobA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00708, cobA, 1 hit |
i Sequence
Sequence statusi: Complete.
P31570-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDERYQQRQ QKVKDRVDAR VAQAQEERGI IIVFTGNGKG KTTAAFGTAA
60 70 80 90 100
RAVGHGKNVG VVQFIKGTWP NGERNLLEPH GVEFQVMATG FTWETQNREA
110 120 130 140 150
DTAACMAVWQ HGKRMLADPL LDMVVLDELT YMVAYDYLPL EEVISALNAR
160 170 180 190
PGHQTVIITG RGCHRDILDL ADTVSELRPV KHAFDAGVKA QMGIDY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08890 Unassigned DNA Translation: AAA71929.1 AE006468 Genomic DNA Translation: AAL20636.1 |
PIRi | JN0721 |
RefSeqi | NP_460677.1, NC_003197.2 WP_001278856.1, NC_003197.2 |
Genome annotation databases
EnsemblBacteriai | AAL20636; AAL20636; STM1718 |
GeneIDi | 1253237 |
KEGGi | stm:STM1718 |
PATRICi | fig|99287.12.peg.1815 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L08890 Unassigned DNA Translation: AAA71929.1 AE006468 Genomic DNA Translation: AAL20636.1 |
PIRi | JN0721 |
RefSeqi | NP_460677.1, NC_003197.2 WP_001278856.1, NC_003197.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G5R | X-ray | 2.10 | A | 1-196 | [»] | |
1G5T | X-ray | 1.80 | A | 1-196 | [»] | |
1G64 | X-ray | 2.10 | A/B | 1-196 | [»] | |
4HUT | X-ray | 1.95 | A/B | 6-196 | [»] | |
AlphaFoldDBi | P31570 | |||||
SMRi | P31570 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 99287.STM1718 |
Proteomic databases
PaxDbi | P31570 |
Genome annotation databases
EnsemblBacteriai | AAL20636; AAL20636; STM1718 |
GeneIDi | 1253237 |
KEGGi | stm:STM1718 |
PATRICi | fig|99287.12.peg.1815 |
Phylogenomic databases
HOGENOMi | CLU_088595_0_0_6 |
OMAi | HAMGEGF |
PhylomeDBi | P31570 |
Enzyme and pathway databases
UniPathwayi | UPA00148;UER00233 |
BRENDAi | 2.5.1.17, 2169 |
Miscellaneous databases
EvolutionaryTracei | P31570 |
Family and domain databases
CDDi | cd00561, CobA_CobO_BtuR, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003724, CblAdoTrfase_CobA IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02572, CobA_CobO_BtuR, 1 hit |
PIRSFi | PIRSF015617, Adensltrnsf_CobA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00708, cobA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BTUR_SALTY | |
Accessioni | P31570Primary (citable) accession number: P31570 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families