UniProtKB - P31554 (LPTD_ECOLI)
Protein
LPS-assembly protein LptD
Gene
lptD
Organism
Escherichia coli (strain K12)
Status
Functioni
Together with LptE, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane. Contributes to n-hexane resistance.UniRule annotation8 Publications
Miscellaneous
The correct assembly of LptD depends on the thiol:disulfide interchange proteins DsbA and DsbC, the chaperone proteins SurA and Skp, LptE and the chaperone/protease BepA (PubMed:20566849, PubMed:20615876, PubMed:21257904, PubMed:21257909, PubMed:23772069, PubMed:24003122). Incorrectly folded LptD can be degraded by three different proteases, DegP, BepA and YcaL. These proteases act at distinct points in the outer membrane protein assembly (PubMed:24003122, PubMed:28784813).7 Publications
GO - Biological processi
- Gram-negative-bacterium-type cell outer membrane assembly Source: EcoCyc
- lipopolysaccharide export Source: GO_Central
- response to organic substance Source: InterPro
Enzyme and pathway databases
BioCyci | EcoCyc:EG11569-MONOMER MetaCyc:EG11569-MONOMER |
Protein family/group databases
TCDBi | 1.B.42.1.2, the outer membrane lipopolysaccharide export porin (lps-ep) family |
Names & Taxonomyi
Protein namesi | Recommended name: LPS-assembly protein LptDUniRule annotationAlternative name(s): Organic solvent tolerance protein |
Gene namesi | Name:lptDUniRule annotation Synonyms:imp, ostA, yabG Ordered Locus Names:b0054, JW0053 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell outer membrane UniRule annotation4 Publications
GO - Cellular componenti
- cell outer membrane Source: EcoCyc
- transporter complex Source: EcoCyc
Keywords - Cellular componenti
Cell outer membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutations alter the permeability of the outer membrane resulting in increased sensitivity to detergents, antibiotics and dyes. Depletion leads to filamentation followed by membrane rupture and cell lysis.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 529 – 538 | Missing : Impairs assembly of the LptD/LptE complex. Does not form native disulfide bonds. Severely compromises outer membrane integrity. 1 Publication | 10 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | UniRule annotation2 PublicationsAdd BLAST | 24 | |
ChainiPRO_0000020276 | 25 – 784 | LPS-assembly protein LptDAdd BLAST | 760 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 724 | |||
Disulfide bondi | 173 ↔ 725 |
Post-translational modificationi
Contains two intramolecular disulfide bonds. At least one disulfide bond is required for activity, and protein is probably fully oxidized in vivo.
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P31554 |
PaxDbi | P31554 |
PRIDEi | P31554 |
2D gel databases
SWISS-2DPAGEi | P31554 |
PTM databases
CarbonylDBi | P31554 |
Expressioni
Inductioni
Constitutively expressed.1 Publication
Interactioni
Subunit structurei
Component of the lipopolysaccharide transport and assembly complex.
Interacts with LptE and LptA. May interact with LptE during assembly of LptD by the beta-barrel assembly machine.
UniRule annotation6 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4259723, 266 interactors |
ComplexPortali | CPX-1093, LptDE outer membrane translocon complex |
DIPi | DIP-10029N |
IntActi | P31554, 8 interactors |
STRINGi | 511145.b0054 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P31554 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
Contains an N-terminal soluble domain that is likely periplasmic and interacts with LptA, and a C-terminal transmembrane domain, which is predicted to be a beta-barrel and interacts with LptE. Residues 529-538 play a key role in interaction with LptE.3 Publications
Sequence similaritiesi
Belongs to the LptD family.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1452, Bacteria |
HOGENOMi | CLU_009039_2_0_6 |
InParanoidi | P31554 |
PhylomeDBi | P31554 |
Family and domain databases
HAMAPi | MF_01411, LPS_assembly_LptD, 1 hit |
InterProi | View protein in InterPro IPR020889, LipoPS_assembly_LptD IPR007543, LptD_C IPR005653, OstA-like_N |
Pfami | View protein in Pfam PF04453, LptD, 1 hit PF03968, LptD_N, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P31554-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKRIPTLLA TMIATALYSQ QGLAADLASQ CMLGVPSYDR PLVQGDTNDL
60 70 80 90 100
PVTINADHAK GDYPDDAVFT GSVDIMQGNS RLQADEVQLH QKEAPGQPEP
110 120 130 140 150
VRTVDALGNV HYDDNQVILK GPKGWANLNT KDTNVWEGDY QMVGRQGRGK
160 170 180 190 200
ADLMKQRGEN RYTILDNGSF TSCLPGSDTW SVVGSEIIHD REEQVAEIWN
210 220 230 240 250
ARFKVGPVPI FYSPYLQLPV GDKRRSGFLI PNAKYTTTNY FEFYLPYYWN
260 270 280 290 300
IAPNMDATIT PHYMHRRGNI MWENEFRYLS QAGAGLMELD YLPSDKVYED
310 320 330 340 350
EHPNDDSSRR WLFYWNHSGV MDQVWRFNVD YTKVSDPSYF NDFDNKYGSS
360 370 380 390 400
TDGYATQKFS VGYAVQNFNA TVSTKQFQVF SEQNTSSYSA EPQLDVNYYQ
410 420 430 440 450
NDVGPFDTRI YGQAVHFVNT RDDMPEATRV HLEPTINLPL SNNWGSINTE
460 470 480 490 500
AKLLATHYQQ TNLDWYNSRN TTKLDESVNR VMPQFKVDGK MVFERDMEML
510 520 530 540 550
APGYTQTLEP RAQYLYVPYR DQSDIYNYDS SLLQSDYSGL FRDRTYGGLD
560 570 580 590 600
RIASANQVTT GVTSRIYDDA AVERFNISVG QIYYFTESRT GDDNITWEND
610 620 630 640 650
DKTGSLVWAG DTYWRISERW GLRGGIQYDT RLDNVATSNS SIEYRRDEDR
660 670 680 690 700
LVQLNYRYAS PEYIQATLPK YYSTAEQYKN GISQVGAVAS WPIADRWSIV
710 720 730 740 750
GAYYYDTNAN KQADSMLGVQ YSSCCYAIRV GYERKLNGWD NDKQHAVYDN
760 770 780
AIGFNIELRG LSSNYGLGTQ EMLRSNILPY QNTL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB013134 Genomic DNA Translation: BAA34130.1 U00096 Genomic DNA Translation: AAC73165.1 AP009048 Genomic DNA Translation: BAB96622.1 |
PIRi | F64726 |
RefSeqi | NP_414596.1, NC_000913.3 WP_000746151.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73165; AAC73165; b0054 BAB96622; BAB96622; BAB96622 |
GeneIDi | 945011 |
KEGGi | ecj:JW0053 eco:b0054 |
PATRICi | fig|1411691.4.peg.2229 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB013134 Genomic DNA Translation: BAA34130.1 U00096 Genomic DNA Translation: AAC73165.1 AP009048 Genomic DNA Translation: BAB96622.1 |
PIRi | F64726 |
RefSeqi | NP_414596.1, NC_000913.3 WP_000746151.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4RHB | X-ray | 3.35 | A/C | 203-784 | [»] | |
SMRi | P31554 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259723, 266 interactors |
ComplexPortali | CPX-1093, LptDE outer membrane translocon complex |
DIPi | DIP-10029N |
IntActi | P31554, 8 interactors |
STRINGi | 511145.b0054 |
Protein family/group databases
TCDBi | 1.B.42.1.2, the outer membrane lipopolysaccharide export porin (lps-ep) family |
PTM databases
CarbonylDBi | P31554 |
2D gel databases
SWISS-2DPAGEi | P31554 |
Proteomic databases
jPOSTi | P31554 |
PaxDbi | P31554 |
PRIDEi | P31554 |
Genome annotation databases
EnsemblBacteriai | AAC73165; AAC73165; b0054 BAB96622; BAB96622; BAB96622 |
GeneIDi | 945011 |
KEGGi | ecj:JW0053 eco:b0054 |
PATRICi | fig|1411691.4.peg.2229 |
Organism-specific databases
EchoBASEi | EB1529 |
Phylogenomic databases
eggNOGi | COG1452, Bacteria |
HOGENOMi | CLU_009039_2_0_6 |
InParanoidi | P31554 |
PhylomeDBi | P31554 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11569-MONOMER MetaCyc:EG11569-MONOMER |
Miscellaneous databases
PROi | PR:P31554 |
Family and domain databases
HAMAPi | MF_01411, LPS_assembly_LptD, 1 hit |
InterProi | View protein in InterPro IPR020889, LipoPS_assembly_LptD IPR007543, LptD_C IPR005653, OstA-like_N |
Pfami | View protein in Pfam PF04453, LptD, 1 hit PF03968, LptD_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LPTD_ECOLI | |
Accessioni | P31554Primary (citable) accession number: P31554 Secondary accession number(s): P75631 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families