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Protein

Crotonobetaine/carnitine--CoA ligase

Gene

caiC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle. Also has activity toward crotonobetaine and gamma-butyrobetaine.UniRule annotation1 Publication

Catalytic activityi

ATP + 4-(trimethylamino)butanoate + CoA = AMP + diphosphate + gamma-butyrobetainyl-CoA.UniRule annotation1 Publication
ATP + crotonobetaine + CoA = AMP + diphosphate + crotonobetainyl-CoA.UniRule annotation1 Publication
ATP + L-carnitine + CoA = AMP + diphosphate + L-carnitinyl-CoA.UniRule annotation1 Publication

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase

Enzyme and pathway databases

BioCyciEcoCyc:CAIC-MONOMER
MetaCyc:CAIC-MONOMER
UniPathwayi
UPA00117

Protein family/group databases

TCDBi4.C.1.1.6 the fatty acid transporter (fat) family

Names & Taxonomyi

Protein namesi
Recommended name:
Crotonobetaine/carnitine--CoA ligaseUniRule annotationCurated (EC:6.2.1.48UniRule annotation1 Publication)
Alternative name(s):
Betaine:CoA ligase1 Publication
Gene namesi
Name:caiCUniRule annotation
Synonyms:yaaM
Ordered Locus Names:b0037, JW0036
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11558 caiC

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001930651 – 517Crotonobetaine/carnitine--CoA ligaseAdd BLAST517

Proteomic databases

EPDiP31552
PaxDbiP31552
PRIDEiP31552

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
allRP0ACN43EBI-1112975,EBI-561736

Protein-protein interaction databases

BioGridi4261017, 531 interactors
DIPiDIP-9243N
IntActiP31552, 5 interactors
STRINGi316385.ECDH10B_0038

Structurei

3D structure databases

ProteinModelPortaliP31552
SMRiP31552
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105CEY Bacteria
COG0318 LUCA
HOGENOMiHOG000230001
InParanoidiP31552
KOiK02182
OMAiWLMQRAF
PhylomeDBiP31552

Family and domain databases

HAMAPiMF_01524 CaiC, 1 hit
InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023456 CaiC
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P31552-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDIIGGQHLR QMWDDLADVY GHKTALICES SGGVVNRYSY LELNQEINRT
60 70 80 90 100
ANLFYTLGIR KGDKVALHLD NCPEFIFCWF GLAKIGAIMV PINARLLCEE
110 120 130 140 150
SAWILQNSQA CLLVTSAQFY PMYQQIQQED ATQLRHICLT DVALPADDGV
160 170 180 190 200
SSFTQLKNQQ PATLCYAPPL STDDTAEILF TSGTTSRPKG VVITHYNLRF
210 220 230 240 250
AGYYSAWQCA LRDDDVYLTV MPAFHIDCQC TAAMAAFSAG ATFVLVEKYS
260 270 280 290 300
ARAFWGQVQK YRATVTECIP MMIRTLMVQP PSANDQQHRL REVMFYLNLS
310 320 330 340 350
EQEKDAFCER FGVRLLTSYG MTETIVGIIG DRPGDKRRWP SIGRVGFCYE
360 370 380 390 400
AEIRDDHNRP LPAGEIGEIC IKGIPGKTIF KEYFLNPQAT AKVLEADGWL
410 420 430 440 450
HTGDTGYRDE EDFFYFVDRR CNMIKRGGEN VSCVELENII AAHPKIQDIV
460 470 480 490 500
VVGIKDSIRD EAIKAFVVLN EGETLSEEEF FRFCEQNMAK FKVPSYLEIR
510
KDLPRNCSGK IIRKNLK
Length:517
Mass (Da):58,559
Last modified:August 21, 2007 - v2
Checksum:iC7A307D16200D8E9
GO

Sequence cautioni

The sequence BAB96606 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA52113 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti98C → R in strain: O44:K74. 1
Natural varianti252 – 253RA → PR in strain: O44:K74. 2
Natural varianti286Q → R in strain: O44:K74 and B. 1
Natural varianti328I → S in strain: O44:K74. 1
Natural varianti345V → A in strain: O44:K74 and B. 1
Natural varianti359R → C in strain: B. 1
Natural varianti374I → V in strain: O44:K74 and B. 1
Natural varianti388Q → K in strain: O44:K74 and B. 1
Natural varianti396A → V in strain: O44:K74. 1
Natural varianti408R → C in strain: O44:K74. 1
Natural varianti412D → G in strain: O44:K74 and B. 1
Natural varianti442A → T in strain: O44:K74 and B. 1
Natural varianti503L → LDL in strain: O44:K74. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA Translation: CAA52113.1 Different initiation.
U00096 Genomic DNA Translation: AAC73148.2
AP009048 Genomic DNA Translation: BAB96606.1 Different initiation.
AY625099 Genomic DNA Translation: AAT42453.1
PIRiE64724 S40558
RefSeqiNP_414579.4, NC_000913.3
WP_001350478.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73148; AAC73148; b0037
BAB96606; BAB96606; BAB96606
GeneIDi944886
KEGGiecj:JW0036
eco:b0037
PATRICifig|1411691.4.peg.2246

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA Translation: CAA52113.1 Different initiation.
U00096 Genomic DNA Translation: AAC73148.2
AP009048 Genomic DNA Translation: BAB96606.1 Different initiation.
AY625099 Genomic DNA Translation: AAT42453.1
PIRiE64724 S40558
RefSeqiNP_414579.4, NC_000913.3
WP_001350478.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP31552
SMRiP31552
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261017, 531 interactors
DIPiDIP-9243N
IntActiP31552, 5 interactors
STRINGi316385.ECDH10B_0038

Protein family/group databases

TCDBi4.C.1.1.6 the fatty acid transporter (fat) family

Proteomic databases

EPDiP31552
PaxDbiP31552
PRIDEiP31552

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73148; AAC73148; b0037
BAB96606; BAB96606; BAB96606
GeneIDi944886
KEGGiecj:JW0036
eco:b0037
PATRICifig|1411691.4.peg.2246

Organism-specific databases

EchoBASEiEB1519
EcoGeneiEG11558 caiC

Phylogenomic databases

eggNOGiENOG4105CEY Bacteria
COG0318 LUCA
HOGENOMiHOG000230001
InParanoidiP31552
KOiK02182
OMAiWLMQRAF
PhylomeDBiP31552

Enzyme and pathway databases

UniPathwayi
UPA00117

BioCyciEcoCyc:CAIC-MONOMER
MetaCyc:CAIC-MONOMER

Miscellaneous databases

PROiPR:P31552

Family and domain databases

HAMAPiMF_01524 CaiC, 1 hit
InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023456 CaiC
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAIC_ECOLI
AccessioniPrimary (citable) accession number: P31552
Secondary accession number(s): Q6IU50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 21, 2007
Last modified: March 28, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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