UniProtKB - P31552 (CAIC_ECOLI)
Protein
Crotonobetaine/carnitine--CoA ligase
Gene
caiC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle. Also has activity toward crotonobetaine and gamma-butyrobetaine.UniRule annotation1 Publication
Catalytic activityi
- 4-(trimethylamino)butanoate + ATP + CoA = AMP + diphosphate + γ-butyrobetainyl-CoAUniRule annotation1 PublicationEC:6.2.1.48UniRule annotation1 Publication
- EC:6.2.1.48UniRule annotation1 Publication
- EC:6.2.1.48UniRule annotation1 Publication
: carnitine metabolism Pathwayi
This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.
GO - Molecular functioni
- acid-thiol ligase activity Source: InterPro
- carnitine-CoA ligase activity Source: EcoCyc
- CoA-ligase activity Source: GO_Central
- crotonobetaine-CoA ligase activity Source: EcoCyc
GO - Biological processi
- carnitine metabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Ligase |
Enzyme and pathway databases
BioCyci | EcoCyc:CAIC-MONOMER MetaCyc:CAIC-MONOMER |
UniPathwayi | UPA00117 |
Protein family/group databases
TCDBi | 4.C.1.1.6, the fatty acid transporter (fat) family |
Names & Taxonomyi
Protein namesi | Recommended name: Crotonobetaine/carnitine--CoA ligaseUniRule annotationCurated (EC:6.2.1.48UniRule annotation1 Publication)Alternative name(s): Betaine:CoA ligase1 Publication |
Gene namesi | Name:caiCUniRule annotation Synonyms:yaaM Ordered Locus Names:b0037, JW0036 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193065 | 1 – 517 | Crotonobetaine/carnitine--CoA ligaseAdd BLAST | 517 |
Proteomic databases
PaxDbi | P31552 |
PRIDEi | P31552 |
Interactioni
Binary interactionsi
P31552
With | #Exp. | IntAct |
---|---|---|
allR [P0ACN4] | 3 | EBI-1112975,EBI-561736 |
Protein-protein interaction databases
BioGRIDi | 4261017, 531 interactors 849286, 2 interactors |
DIPi | DIP-9243N |
IntActi | P31552, 5 interactors |
STRINGi | 511145.b0037 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0318, Bacteria |
HOGENOMi | CLU_000022_59_0_6 |
InParanoidi | P31552 |
PhylomeDBi | P31552 |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
HAMAPi | MF_01524, CaiC, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf IPR023456, CaiC |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
P31552-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDIIGGQHLR QMWDDLADVY GHKTALICES SGGVVNRYSY LELNQEINRT
60 70 80 90 100
ANLFYTLGIR KGDKVALHLD NCPEFIFCWF GLAKIGAIMV PINARLLCEE
110 120 130 140 150
SAWILQNSQA CLLVTSAQFY PMYQQIQQED ATQLRHICLT DVALPADDGV
160 170 180 190 200
SSFTQLKNQQ PATLCYAPPL STDDTAEILF TSGTTSRPKG VVITHYNLRF
210 220 230 240 250
AGYYSAWQCA LRDDDVYLTV MPAFHIDCQC TAAMAAFSAG ATFVLVEKYS
260 270 280 290 300
ARAFWGQVQK YRATVTECIP MMIRTLMVQP PSANDQQHRL REVMFYLNLS
310 320 330 340 350
EQEKDAFCER FGVRLLTSYG MTETIVGIIG DRPGDKRRWP SIGRVGFCYE
360 370 380 390 400
AEIRDDHNRP LPAGEIGEIC IKGIPGKTIF KEYFLNPQAT AKVLEADGWL
410 420 430 440 450
HTGDTGYRDE EDFFYFVDRR CNMIKRGGEN VSCVELENII AAHPKIQDIV
460 470 480 490 500
VVGIKDSIRD EAIKAFVVLN EGETLSEEEF FRFCEQNMAK FKVPSYLEIR
510
KDLPRNCSGK IIRKNLK
Sequence cautioni
The sequence BAB96606 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA52113 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 98 | C → R in strain: O44:K74. | 1 | |
Natural varianti | 252 – 253 | RA → PR in strain: O44:K74. | 2 | |
Natural varianti | 286 | Q → R in strain: O44:K74 and B. | 1 | |
Natural varianti | 328 | I → S in strain: O44:K74. | 1 | |
Natural varianti | 345 | V → A in strain: O44:K74 and B. | 1 | |
Natural varianti | 359 | R → C in strain: B. | 1 | |
Natural varianti | 374 | I → V in strain: O44:K74 and B. | 1 | |
Natural varianti | 388 | Q → K in strain: O44:K74 and B. | 1 | |
Natural varianti | 396 | A → V in strain: O44:K74. | 1 | |
Natural varianti | 408 | R → C in strain: O44:K74. | 1 | |
Natural varianti | 412 | D → G in strain: O44:K74 and B. | 1 | |
Natural varianti | 442 | A → T in strain: O44:K74 and B. | 1 | |
Natural varianti | 503 | L → LDL in strain: O44:K74. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73904 Genomic DNA Translation: CAA52113.1 Different initiation. U00096 Genomic DNA Translation: AAC73148.2 AP009048 Genomic DNA Translation: BAB96606.1 Different initiation. AY625099 Genomic DNA Translation: AAT42453.1 |
PIRi | E64724, S40558 |
RefSeqi | NP_414579.4, NC_000913.3 WP_001350478.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73148; AAC73148; b0037 BAB96606; BAB96606; BAB96606 |
GeneIDi | 944886 |
KEGGi | ecj:JW0036 eco:b0037 |
PATRICi | fig|1411691.4.peg.2246 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73904 Genomic DNA Translation: CAA52113.1 Different initiation. U00096 Genomic DNA Translation: AAC73148.2 AP009048 Genomic DNA Translation: BAB96606.1 Different initiation. AY625099 Genomic DNA Translation: AAT42453.1 |
PIRi | E64724, S40558 |
RefSeqi | NP_414579.4, NC_000913.3 WP_001350478.1, NZ_LN832404.1 |
3D structure databases
SMRi | P31552 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261017, 531 interactors 849286, 2 interactors |
DIPi | DIP-9243N |
IntActi | P31552, 5 interactors |
STRINGi | 511145.b0037 |
Protein family/group databases
TCDBi | 4.C.1.1.6, the fatty acid transporter (fat) family |
Proteomic databases
PaxDbi | P31552 |
PRIDEi | P31552 |
Genome annotation databases
EnsemblBacteriai | AAC73148; AAC73148; b0037 BAB96606; BAB96606; BAB96606 |
GeneIDi | 944886 |
KEGGi | ecj:JW0036 eco:b0037 |
PATRICi | fig|1411691.4.peg.2246 |
Organism-specific databases
EchoBASEi | EB1519 |
Phylogenomic databases
eggNOGi | COG0318, Bacteria |
HOGENOMi | CLU_000022_59_0_6 |
InParanoidi | P31552 |
PhylomeDBi | P31552 |
Enzyme and pathway databases
UniPathwayi | UPA00117 |
BioCyci | EcoCyc:CAIC-MONOMER MetaCyc:CAIC-MONOMER |
Miscellaneous databases
PROi | PR:P31552 |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
HAMAPi | MF_01524, CaiC, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf IPR023456, CaiC |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAIC_ECOLI | |
Accessioni | P31552Primary (citable) accession number: P31552 Secondary accession number(s): Q6IU50 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | August 21, 2007 | |
Last modified: | December 2, 2020 | |
This is version 164 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families