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UniProtKB - P31551 (CAID_ECOLI)
Protein
Carnitinyl-CoA dehydratase
Gene
caiD
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA (PubMed:11551212).
Can also hydrate crotonyl-CoA to hydroxybutyryl-CoA (PubMed:11551212).
1 PublicationCatalytic activityi
- EC:4.2.1.149UniRule annotation1 Publication
Kineticsi
- KM=12 µM for crotonobetainyl-CoA1 Publication
- KM=38 µM for crotonyl-CoA1 Publication
- Vmax=186 µmol/min/mg enzyme with crotonobetainyl-CoA as substrate1 Publication
- Vmax=0.3 µmol/min/mg enzyme with crotonyl-CoA as substrate1 Publication
: carnitine metabolism Pathwayi
This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 111 | NucleophileUniRule annotationBy similarity | 1 | |
Active sitei | 131 | Proton acceptorUniRule annotationBy similarity | 1 |
GO - Molecular functioni
- carnitine racemase activity Source: EcoliWiki
- hydro-lyase activity Source: UniProtKB-UniRule
GO - Biological processi
- carnitine catabolic process Source: EcoliWiki
Keywordsi
Molecular function | Lyase |
Enzyme and pathway databases
BioCyci | EcoCyc:CARNRACE-MONOMER |
BRENDAi | 4.2.1.149, 2026 |
SABIO-RKi | P31551 |
UniPathwayi | UPA00117 |
Names & Taxonomyi
Protein namesi | Recommended name: Carnitinyl-CoA dehydrataseUniRule annotationCurated (EC:4.2.1.149UniRule annotation1 Publication)Alternative name(s): Crotonobetainyl-CoA hydratase1 PublicationUniRule annotation |
Gene namesi | Name:caiD1 PublicationUniRule annotation Synonyms:yaaL Ordered Locus Names:b0036, JW0035 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000109348 | 2 – 261 | Carnitinyl-CoA dehydrataseAdd BLAST | 260 |
Proteomic databases
jPOSTi | P31551 |
PaxDbi | P31551 |
PRIDEi | P31551 |
Interactioni
Subunit structurei
Homotrimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4261692, 569 interactors 853243, 1 interactor |
IntActi | P31551, 9 interactors |
STRINGi | 511145.b0036 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1024, Bacteria |
HOGENOMi | CLU_009834_7_6_6 |
InParanoidi | P31551 |
PhylomeDBi | P31551 |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
HAMAPi | MF_01051, CaiD, 1 hit |
InterProi | View protein in InterPro IPR022852, Carnitinyl_CoA_dehydratase IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P31551-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSESLHLTRN GSILEITLDR PKANAIDAKT SFEMGEVFLN FRDDPQLRVA
60 70 80 90 100
IITGAGEKFF SAGWDLKAAA EGEAPDADFG PGGFAGLTEI FNLDKPVIAA
110 120 130 140 150
VNGYAFGGGF ELALAADFIV CADNASFALP EAKLGIVPDS GGVLRLPKIL
160 170 180 190 200
PPAIVNEMVM TGRRMGAEEA LRWGIVNRVV SQAELMDNAR ELAQQLVNSA
210 220 230 240 250
PLAIAALKEI YRTTSEMPVE EAYRYIRSGV LKHYPSVLHS EDAIEGPLAF
260
AEKRDPVWKG R
Sequence cautioni
The sequence BAB96605 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA52114 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 247 | P → L in strain: O44:K74. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73904 Genomic DNA Translation: CAA52114.1 Different initiation. U00096 Genomic DNA Translation: AAC73147.2 AP009048 Genomic DNA Translation: BAB96605.2 Different initiation. |
PIRi | D64724 |
RefSeqi | NP_414578.2, NC_000913.3 WP_001295419.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73147; AAC73147; b0036 BAB96605; BAB96605; BAB96605 |
GeneIDi | 58460825 948995 |
KEGGi | ecj:JW0035 eco:b0036 |
PATRICi | fig|1411691.4.peg.2247 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73904 Genomic DNA Translation: CAA52114.1 Different initiation. U00096 Genomic DNA Translation: AAC73147.2 AP009048 Genomic DNA Translation: BAB96605.2 Different initiation. |
PIRi | D64724 |
RefSeqi | NP_414578.2, NC_000913.3 WP_001295419.1, NZ_LN832404.1 |
3D structure databases
SMRi | P31551 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261692, 569 interactors 853243, 1 interactor |
IntActi | P31551, 9 interactors |
STRINGi | 511145.b0036 |
Proteomic databases
jPOSTi | P31551 |
PaxDbi | P31551 |
PRIDEi | P31551 |
Genome annotation databases
EnsemblBacteriai | AAC73147; AAC73147; b0036 BAB96605; BAB96605; BAB96605 |
GeneIDi | 58460825 948995 |
KEGGi | ecj:JW0035 eco:b0036 |
PATRICi | fig|1411691.4.peg.2247 |
Organism-specific databases
EchoBASEi | EB1518 |
Phylogenomic databases
eggNOGi | COG1024, Bacteria |
HOGENOMi | CLU_009834_7_6_6 |
InParanoidi | P31551 |
PhylomeDBi | P31551 |
Enzyme and pathway databases
UniPathwayi | UPA00117 |
BioCyci | EcoCyc:CARNRACE-MONOMER |
BRENDAi | 4.2.1.149, 2026 |
SABIO-RKi | P31551 |
Miscellaneous databases
PROi | PR:P31551 |
Family and domain databases
Gene3Di | 1.10.12.10, 1 hit |
HAMAPi | MF_01051, CaiD, 1 hit |
InterProi | View protein in InterPro IPR022852, Carnitinyl_CoA_dehydratase IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR014748, Enoyl-CoA_hydra_C |
Pfami | View protein in Pfam PF00378, ECH_1, 1 hit |
SUPFAMi | SSF52096, SSF52096, 1 hit |
PROSITEi | View protein in PROSITE PS00166, ENOYL_COA_HYDRATASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAID_ECOLI | |
Accessioni | P31551Primary (citable) accession number: P31551 Secondary accession number(s): P75623 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 167 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families