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Protein

Thiamine-binding periplasmic protein

Gene

thiB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex ThiBPQ involved in thiamine import.1 Publication

GO - Molecular functioni

  • thiamine binding Source: EcoCyc
  • thiamine pyrophosphate binding Source: EcoCyc

GO - Biological processi

  • thiamine transport Source: EcoCyc
  • transmembrane transport Source: InterPro

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:SFUA-MONOMER
MetaCyc:SFUA-MONOMER

Protein family/group databases

TCDBi3.A.1.19.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine-binding periplasmic protein
Gene namesi
Name:thiB
Synonyms:tbpA, yabL
Ordered Locus Names:b0068, JW0067
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11574 tbpA

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
ChainiPRO_000003170519 – 327Thiamine-binding periplasmic proteinAdd BLAST309

Proteomic databases

EPDiP31550
PaxDbiP31550
PRIDEiP31550

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (ThiQ), two transmembrane proteins (ThiP) and a solute-binding protein (ThiB).Curated

Protein-protein interaction databases

BioGridi4261341, 31 interactors
DIPiDIP-10967N
IntActiP31550, 3 interactors
STRINGi316407.85674313

Chemistry databases

BindingDBiP31550

Structurei

Secondary structure

1327
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 26Combined sources6
Helixi28 – 31Combined sources4
Helixi37 – 45Combined sources9
Beta strandi50 – 56Combined sources7
Helixi60 – 70Combined sources11
Helixi71 – 73Combined sources3
Beta strandi77 – 83Combined sources7
Helixi84 – 86Combined sources3
Helixi87 – 93Combined sources7
Helixi103 – 105Combined sources3
Beta strandi118 – 130Combined sources13
Turni131 – 133Combined sources3
Helixi141 – 146Combined sources6
Beta strandi153 – 156Combined sources4
Turni158 – 160Combined sources3
Helixi162 – 175Combined sources14
Helixi176 – 178Combined sources3
Helixi179 – 187Combined sources9
Beta strandi190 – 196Combined sources7
Helixi197 – 205Combined sources9
Beta strandi210 – 215Combined sources6
Helixi218 – 226Combined sources9
Beta strandi231 – 233Combined sources3
Beta strandi240 – 250Combined sources11
Helixi256 – 266Combined sources11
Helixi269 – 272Combined sources4
Helixi275 – 278Combined sources4
Beta strandi281 – 285Combined sources5
Helixi293 – 295Combined sources3
Beta strandi300 – 303Combined sources4
Helixi307 – 325Combined sources19

3D structure databases

ProteinModelPortaliP31550
SMRiP31550
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31550

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DP9 Bacteria
COG4143 LUCA
HOGENOMiHOG000272499
InParanoidiP31550
KOiK02064
OMAiPTTNWMY
PhylomeDBiP31550

Family and domain databases

CDDicd13545 PBP2_TbpA, 1 hit
InterProiView protein in InterPro
IPR006061 SBP_1_CS
IPR005967 ThiB
IPR005948 ThiB-like
TIGRFAMsiTIGR01254 sfuA, 1 hit
TIGR01276 thiB, 1 hit
PROSITEiView protein in PROSITE
PS01037 SBP_BACTERIAL_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKKCLPLLL LCTAPVFAKP VLTVYTYDSF AADWGPGPVV KKAFEADCNC
60 70 80 90 100
ELKLVALEDG VSLLNRLRME GKNSKADVVL GLDNNLLDAA SKTGLFAKSG
110 120 130 140 150
VAADAVNVPG GWNNDTFVPF DYGYFAFVYD KNKLKNPPQS LKELVESDQN
160 170 180 190 200
WRVIYQDPRT STPGLGLLLW MQKVYGDDAP QAWQKLAKKT VTVTKGWSEA
210 220 230 240 250
YGLFLKGESD LVLSYTTSPA YHILEEKKDN YAAANFSEGH YLQVEVAART
260 270 280 290 300
AASKQPELAQ KFLQFMVSPA FQNAIPTGNW MYPVANVTLP AGFEKLTKPA
310 320
TTLEFTPAEV AAQRQAWISE WQRAVSR
Length:327
Mass (Da):36,163
Last modified:November 1, 1997 - v2
Checksum:i348A5625FFC94597
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 12MLKKC…LLLLC → MSAPAVAV AA sequence (Ref. 1) CuratedAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09984 Genomic DNA Translation: AAA18833.1
U00096 Genomic DNA Translation: AAC73179.1
AP009048 Genomic DNA Translation: BAB96637.2
PIRiD64728
RefSeqiNP_414610.1, NC_000913.3
WP_001301364.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73179; AAC73179; b0068
BAB96637; BAB96637; BAB96637
GeneIDi946306
KEGGiecj:JW0067
eco:b0068
PATRICifig|1411691.4.peg.2214

Similar proteinsi

Entry informationi

Entry nameiTHIB_ECOLI
AccessioniPrimary (citable) accession number: P31550
Secondary accession number(s): P75637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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