UniProtKB - P31549 (THIP_ECOLI)
Protein
Thiamine transport system permease protein ThiP
Gene
thiP
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex ThiBPQ involved in thiamine import (PubMed:12175925). Probably responsible for the translocation of the substrate across the membrane (Probable).Curated1 Publication
Activity regulationi
Transport is inhibited by the sulfhydryl-specific modifier N-ethylmaleimide.1 Publication
GO - Molecular functioni
- thiamine transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- thiamine transmembrane transport Source: EcoCyc
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:SFUB-MONOMER MetaCyc:SFUB-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.19.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Thiamine transport system permease protein ThiP |
Gene namesi | Name:thiP Synonyms:yabK Ordered Locus Names:b0067, JW0066 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 12 – 32 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 58 – 78 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 95 – 115 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 134 – 154 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 199 – 219 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 240 – 260 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 293 – 313 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 334 – 354 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 374 – 394 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 404 – 424 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 463 – 483 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 506 – 526 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060230 | 1 – 536 | Thiamine transport system permease protein ThiPAdd BLAST | 536 |
Proteomic databases
PaxDbi | P31549 |
PRIDEi | P31549 |
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (ThiQ), two transmembrane proteins (ThiP) and a solute-binding protein (ThiB).
By similarityProtein-protein interaction databases
BioGRIDi | 4263358, 12 interactors 849186, 1 interactor |
ComplexPortali | CPX-4387, Thiamine ABC transporter complex |
DIPi | DIP-10989N |
IntActi | P31549, 1 interactor |
STRINGi | 511145.b0067 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 56 – 261 | ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST | 206 | |
Domaini | 331 – 525 | ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST | 195 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1178, Bacteria |
HOGENOMi | CLU_021838_5_3_6 |
InParanoidi | P31549 |
PhylomeDBi | P31549 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 2 hits |
Gene3Di | 1.10.3720.10, 2 hits |
InterProi | View protein in InterPro IPR000515, MetI-like IPR035906, MetI-like_sf IPR005947, ThiP_ABC_transpt |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 2 hits |
SUPFAMi | SSF161098, SSF161098, 2 hits |
TIGRFAMsi | TIGR01253, thiP, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 2 hits |
i Sequence
Sequence statusi: Complete.
P31549-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATRRQPLIP GWLIPGVSAT TLVVAVALAA FLALWWNAPQ DDWVAVWQDS
60 70 80 90 100
YLWHVVRFSF WQAFLSALLS VIPAIFLARA LYRRRFPGRL ALLRLCAMTL
110 120 130 140 150
ILPVLVAVFG ILSVYGRQGW LATLCQSLGL EWTFSPYGLQ GILLAHVFFN
160 170 180 190 200
LPMASRLLLQ ALENIPGEQR QLAAQLGMRS WHFFRFVEWP WLRRQIPPVA
210 220 230 240 250
ALIFMLCFAS FATVLSLGGG PQATTIELAI YQALSYDYDP ARAAMLALLQ
260 270 280 290 300
MVCCLGLVLL SQRLSKAIAP GTTLLQGWRD PDDRLHSRIC DTVLIVLALL
310 320 330 340 350
LLLPPLLAVI VDGVNRQLPE VLAQPVLWQA LWTSLRIALA AGVLCVVLTM
360 370 380 390 400
MLLWSSRELR ARQKMLAGQV LEMSGMLILA MPGIVLATGF FLLLNNTIGL
410 420 430 440 450
PQSADGIVIF TNALMAIPYA LKVLENPMRD ITARYSMLCQ SLGIEGWSRL
460 470 480 490 500
KVVELRALKR PLAQALAFAC VLSIGDFGVV ALFGNDDFRT LPFYLYQQIG
510 520 530
SYRSQDGAVT ALILLLLCFL LFTVIEKLPG RNVKTD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73178.1 AP009048 Genomic DNA Translation: BAB96636.2 |
PIRi | C64728 |
RefSeqi | NP_414609.1, NC_000913.3 WP_000235721.1, NZ_STEB01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC73178; AAC73178; b0067 BAB96636; BAB96636; BAB96636 |
GeneIDi | 944784 |
KEGGi | ecj:JW0066 eco:b0067 |
PATRICi | fig|1411691.4.peg.2215 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73178.1 AP009048 Genomic DNA Translation: BAB96636.2 |
PIRi | C64728 |
RefSeqi | NP_414609.1, NC_000913.3 WP_000235721.1, NZ_STEB01000010.1 |
3D structure databases
SMRi | P31549 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263358, 12 interactors 849186, 1 interactor |
ComplexPortali | CPX-4387, Thiamine ABC transporter complex |
DIPi | DIP-10989N |
IntActi | P31549, 1 interactor |
STRINGi | 511145.b0067 |
Protein family/group databases
TCDBi | 3.A.1.19.1, the atp-binding cassette (abc) superfamily |
Proteomic databases
PaxDbi | P31549 |
PRIDEi | P31549 |
Genome annotation databases
EnsemblBacteriai | AAC73178; AAC73178; b0067 BAB96636; BAB96636; BAB96636 |
GeneIDi | 944784 |
KEGGi | ecj:JW0066 eco:b0067 |
PATRICi | fig|1411691.4.peg.2215 |
Organism-specific databases
EchoBASEi | EB1533 |
Phylogenomic databases
eggNOGi | COG1178, Bacteria |
HOGENOMi | CLU_021838_5_3_6 |
InParanoidi | P31549 |
PhylomeDBi | P31549 |
Enzyme and pathway databases
BioCyci | EcoCyc:SFUB-MONOMER MetaCyc:SFUB-MONOMER |
Miscellaneous databases
PROi | PR:P31549 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 2 hits |
Gene3Di | 1.10.3720.10, 2 hits |
InterProi | View protein in InterPro IPR000515, MetI-like IPR035906, MetI-like_sf IPR005947, ThiP_ABC_transpt |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 2 hits |
SUPFAMi | SSF161098, SSF161098, 2 hits |
TIGRFAMsi | TIGR01253, thiP, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THIP_ECOLI | |
Accessioni | P31549Primary (citable) accession number: P31549 Secondary accession number(s): P75636 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families