UniProtKB - P31547 (METI_ECOLI)
Protein
D-methionine transport system permease protein MetI
Gene
metI
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the binding-protein-dependent transport system for D-methionine and the toxic methionine analog alpha-methyl-methionine. Probably responsible for the translocation of the substrate across the membrane.1 Publication
(Microbial infection) Probably transports the toxic C-terminal region of CdiA from E.coli strain MHI813 across the inner membrane to the cytoplasm, where CdiA has a toxic effect. Toxin transport is strain-specific, mutations in this gene do not confer resistance to several other tested CdiA toxins.1 Publication
GO - Molecular functioni
- L-methionine transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- amino acid transport Source: GO_Central
- D-methionine transport Source: CACAO
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:METI-MONOMER MetaCyc:METI-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.24.1, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: D-methionine transport system permease protein MetI |
Gene namesi | Name:metI Synonyms:yaeE Ordered Locus Names:b0198, JW0194 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | PeriplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 20 – 40 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 41 – 57 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 58 – 78 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 79 – 80 | PeriplasmicSequence analysis | 2 | |
Transmembranei | 81 – 101 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 102 – 151 | CytoplasmicSequence analysisAdd BLAST | 50 | |
Transmembranei | 152 – 172 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 173 – 185 | PeriplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 186 – 206 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 207 – 217 | Cytoplasmic1 PublicationAdd BLAST | 11 |
GO - Cellular componenti
- ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing Source: EcoCyc
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Disruption confers resistance to cellular contact-dependent growth inhibition (CDI) CdiA of E.coli strain MHI813, but not to several other tested CdiA toxins.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060098 | 1 – 217 | D-methionine transport system permease protein MetIAdd BLAST | 217 |
Proteomic databases
jPOSTi | P31547 |
PaxDbi | P31547 |
PRIDEi | P31547 |
Interactioni
Binary interactionsi
P31547
With | #Exp. | IntAct |
---|---|---|
metN [P30750] | 5 | EBI-8769561,EBI-541886 |
Protein-protein interaction databases
BioGRIDi | 4259754, 9 interactors |
ComplexPortali | CPX-2114, Methionine ABC transporter complex |
DIPi | DIP-11198N |
IntActi | P31547, 2 interactors |
STRINGi | 511145.b0198 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P31547 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P31547 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 204 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 192 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2011, Bacteria |
HOGENOMi | CLU_077375_0_1_6 |
InParanoidi | P31547 |
PhylomeDBi | P31547 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR000515, MetI-like IPR035906, MetI-like_sf |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
i Sequence
Sequence statusi: Complete.
P31547-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEPMMWLLV RGVWETLAMT FVSGFFGFVI GLPVGVLLYV TRPGQIIANA
60 70 80 90 100
KLYRTVSAIV NIFRSIPFII LLVWMIPFTR VIVGTSIGLQ AAIVPLTVGA
110 120 130 140 150
APFIARMVEN ALLEIPTGLI EASRAMGATP MQIVRKVLLP EALPGLVNAA
160 170 180 190 200
TITLITLVGY SAMGGAVGAG GLGQIGYQYG YIGYNATVMN TVLVLLVILV
210
YLIQFAGDRI VRAVTRK
Sequence cautioni
The sequence BAA03658 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D15061 Genomic DNA Translation: BAA03658.1 Different initiation. U70214 Genomic DNA Translation: AAB08626.1 U00096 Genomic DNA Translation: AAC73309.1 AP009048 Genomic DNA Translation: BAA77875.1 |
PIRi | F64744 |
RefSeqi | NP_414740.1, NC_000913.3 WP_001294600.1, NZ_STEB01000032.1 |
Genome annotation databases
EnsemblBacteriai | AAC73309; AAC73309; b0198 BAA77875; BAA77875; BAA77875 |
GeneIDi | 52075518 944894 |
KEGGi | ecj:JW0194 eco:b0198 |
PATRICi | fig|1411691.4.peg.2080 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D15061 Genomic DNA Translation: BAA03658.1 Different initiation. U70214 Genomic DNA Translation: AAB08626.1 U00096 Genomic DNA Translation: AAC73309.1 AP009048 Genomic DNA Translation: BAA77875.1 |
PIRi | F64744 |
RefSeqi | NP_414740.1, NC_000913.3 WP_001294600.1, NZ_STEB01000032.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3DHW | X-ray | 3.70 | A/B/E/F | 1-217 | [»] | |
3TUI | X-ray | 2.90 | A/B/E/F | 1-217 | [»] | |
3TUJ | X-ray | 4.00 | A/B | 1-217 | [»] | |
3TUZ | X-ray | 3.40 | A/B/E/F | 1-217 | [»] | |
6CVL | X-ray | 2.95 | A/B | 1-215 | [»] | |
SMRi | P31547 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259754, 9 interactors |
ComplexPortali | CPX-2114, Methionine ABC transporter complex |
DIPi | DIP-11198N |
IntActi | P31547, 2 interactors |
STRINGi | 511145.b0198 |
Protein family/group databases
TCDBi | 3.A.1.24.1, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P31547 |
PaxDbi | P31547 |
PRIDEi | P31547 |
Genome annotation databases
EnsemblBacteriai | AAC73309; AAC73309; b0198 BAA77875; BAA77875; BAA77875 |
GeneIDi | 52075518 944894 |
KEGGi | ecj:JW0194 eco:b0198 |
PATRICi | fig|1411691.4.peg.2080 |
Organism-specific databases
EchoBASEi | EB1688 |
Phylogenomic databases
eggNOGi | COG2011, Bacteria |
HOGENOMi | CLU_077375_0_1_6 |
InParanoidi | P31547 |
PhylomeDBi | P31547 |
Enzyme and pathway databases
BioCyci | EcoCyc:METI-MONOMER MetaCyc:METI-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P31547 |
PROi | PR:P31547 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR000515, MetI-like IPR035906, MetI-like_sf |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | METI_ECOLI | |
Accessioni | P31547Primary (citable) accession number: P31547 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families