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UniProtKB - P31545 (EFEB_ECOLI)
Protein
Deferrochelatase/peroxidase EfeB
Gene
efeB
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the recovery of exogenous heme iron. Extracts iron from heme while preserving the tetrapyrrol ring intact. Also displays peroxidase activity on guaiacol in vitro.
2 PublicationsCofactori
heme b1 PublicationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group non-covalently per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 329 | Iron (heme proximal ligand); via tele nitrogen | 1 | |
Binding sitei | 347 | Heme | 1 |
GO - Molecular functioni
- heme binding Source: EcoliWiki
- metal ion binding Source: UniProtKB-KW
- peroxidase activity Source: EcoliWiki
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- iron import into cell Source: InterPro
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11735-MONOMER |
Protein family/group databases
PeroxiBasei | 5870, EcoDyPrx01_K12 |
TCDBi | 2.A.108.2.3, the iron/lead transporter (ilt) family |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:efeB Synonyms:ycdB Ordered Locus Names:b1019, JW1004 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm 1 Publication
Cytosol
- cytosol Source: GO_Central
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
- periplasmic space Source: EcoliWiki
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 35 | Tat-type signalAdd BLAST | 35 | |
ChainiPRO_0000013817 | 36 – 423 | Deferrochelatase/peroxidase EfeBAdd BLAST | 388 |
Post-translational modificationi
Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. Can also be exported by the Sec system.
Proteomic databases
PaxDbi | P31545 |
PRIDEi | P31545 |
Expressioni
Inductioni
Repressed by Fur in the presence of iron. Repressed at high pH by the two-component regulatory system CpxA/CpxR.2 Publications
Interactioni
Subunit structurei
Homodimer.
Part of a ferrous iron transporter composed of EfeU, EfeO and EfeB. However, this EfeUOB tripartite iron transporter is defective in E.coli strain K12 due to a frameshift mutation in EfeU.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 4263253, 25 interactors |
IntActi | P31545, 8 interactors |
STRINGi | 511145.b1019 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P31545 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P31545 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 236 – 238 | Heme binding | 3 | |
Regioni | 334 – 336 | Heme binding | 3 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG2837, Bacteria |
HOGENOMi | CLU_039488_0_0_6 |
InParanoidi | P31545 |
PhylomeDBi | P31545 |
Family and domain databases
InterProi | View protein in InterPro IPR011008, Dimeric_a/b-barrel IPR006314, Dyp_peroxidase IPR006313, EfeB IPR006311, TAT_signal |
Pfami | View protein in Pfam PF04261, Dyp_perox, 1 hit |
SUPFAMi | SSF54909, SSF54909, 1 hit |
TIGRFAMsi | TIGR01413, Dyp_perox_fam, 1 hit TIGR01412, tat_substr_1, 1 hit |
PROSITEi | View protein in PROSITE PS51404, DYP_PEROXIDASE, 1 hit PS51318, TAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P31545-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQYKDENGVN EPSRRRLLKV IGALALAGSC PVAHAQKTQS APGTLSPDAR
60 70 80 90 100
NEKQPFYGEH QAGILTPQQA AMMLVAFDVL ASDKADLERL FRLLTQRFAF
110 120 130 140 150
LTQGGAAPET PNPRLPPLDS GILGGYIAPD NLTITLSVGH SLFDERFGLA
160 170 180 190 200
PQMPKKLQKM TRFPNDSLDA ALCHGDVLLQ ICANTQDTVI HALRDIIKHT
210 220 230 240 250
PDLLSVRWKR EGFISDHAAR SKGKETPINL LGFKDGTANP DSQNDKLMQK
260 270 280 290 300
VVWVTADQQE PAWTIGGSYQ AVRLIQFRVE FWDRTPLKEQ QTIFGRDKQT
310 320 330 340 350
GAPLGMQHEH DVPDYASDPE GKVIALDSHI RLANPRTAES ESSLMLRRGY
360 370 380 390 400
SYSLGVTNSG QLDMGLLFVC YQHDLEKGFL TVQKRLNGEA LEEYVKPIGG
410 420
GYFFALPGVK DANDYFGSAL LRV
Mass spectrometryi
Molecular mass is 43814 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74104.1 AP009048 Genomic DNA Translation: BAA35800.1 D10391 Genomic DNA Translation: BAA01229.1 |
PIRi | A64844 |
RefSeqi | NP_415538.1, NC_000913.3 WP_001199471.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC74104; AAC74104; b1019 BAA35800; BAA35800; BAA35800 |
GeneIDi | 946500 |
KEGGi | ecj:JW1004 eco:b1019 |
PATRICi | fig|1411691.4.peg.1250 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74104.1 AP009048 Genomic DNA Translation: BAA35800.1 D10391 Genomic DNA Translation: BAA01229.1 |
PIRi | A64844 |
RefSeqi | NP_415538.1, NC_000913.3 WP_001199471.1, NZ_SSZK01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2Y4D | X-ray | 2.00 | A/B | 36-423 | [»] | |
2Y4E | X-ray | 2.30 | A/B | 36-423 | [»] | |
2Y4F | X-ray | 2.70 | A/B | 36-423 | [»] | |
SMRi | P31545 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263253, 25 interactors |
IntActi | P31545, 8 interactors |
STRINGi | 511145.b1019 |
Protein family/group databases
PeroxiBasei | 5870, EcoDyPrx01_K12 |
TCDBi | 2.A.108.2.3, the iron/lead transporter (ilt) family |
Proteomic databases
PaxDbi | P31545 |
PRIDEi | P31545 |
Genome annotation databases
EnsemblBacteriai | AAC74104; AAC74104; b1019 BAA35800; BAA35800; BAA35800 |
GeneIDi | 946500 |
KEGGi | ecj:JW1004 eco:b1019 |
PATRICi | fig|1411691.4.peg.1250 |
Organism-specific databases
EchoBASEi | EB1686 |
Phylogenomic databases
eggNOGi | COG2837, Bacteria |
HOGENOMi | CLU_039488_0_0_6 |
InParanoidi | P31545 |
PhylomeDBi | P31545 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11735-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P31545 |
PROi | PR:P31545 |
Family and domain databases
InterProi | View protein in InterPro IPR011008, Dimeric_a/b-barrel IPR006314, Dyp_peroxidase IPR006313, EfeB IPR006311, TAT_signal |
Pfami | View protein in Pfam PF04261, Dyp_perox, 1 hit |
SUPFAMi | SSF54909, SSF54909, 1 hit |
TIGRFAMsi | TIGR01413, Dyp_perox_fam, 1 hit TIGR01412, tat_substr_1, 1 hit |
PROSITEi | View protein in PROSITE PS51404, DYP_PEROXIDASE, 1 hit PS51318, TAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EFEB_ECOLI | |
Accessioni | P31545Primary (citable) accession number: P31545 Secondary accession number(s): P75903 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families