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Protein

Phospholipase A2, membrane associated

Gene

Pla2g2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:8425615). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner (By similarity).By similarity1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Calcium; via carbonyl oxygenBy similarity1
Metal bindingi50Calcium; via carbonyl oxygenBy similarity1
Metal bindingi52Calcium; via carbonyl oxygenBy similarity1
Active sitei68By similarity1
Metal bindingi69CalciumBy similarity1
Active sitei113By similarity1
Sitei122Important for integrin bindingBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processGrowth regulation, Lipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2, membrane associated (EC:3.1.1.41 Publication)
Alternative name(s):
Enhancing factor
Short name:
EF
GIIC sPLA2
Group IIA phospholipase A2
Phosphatidylcholine 2-acylhydrolase 2A
Gene namesi
Name:Pla2g2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104642 Pla2g2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5761

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000002275122 – 146Phospholipase A2, membrane associatedAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 139By similarity
Disulfide bondi49 ↔ 65By similarity
Disulfide bondi64 ↔ 119By similarity
Disulfide bondi70 ↔ 146By similarity
Disulfide bondi71 ↔ 112By similarity
Disulfide bondi80 ↔ 105By similarity
Disulfide bondi98 ↔ 110By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP31482

PTM databases

PhosphoSitePlusiP31482

Expressioni

Tissue specificityi

Mainly in the Paneth cells adjacent to the stem population in the small intestines. Also expressed in regenerating liver and hyperplastic esophageal epithelium.

Interactioni

Protein-protein interaction databases

BioGridi202219, 4 interactors

Chemistry databases

BindingDBiP31482

Structurei

3D structure databases

ProteinModelPortaliP31482
SMRiP31482
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137
InParanoidiP31482
KOiK01047
PhylomeDBiP31482

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

P31482-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVLLLLAAS IMAFGSIQVQ GNIAQFGEMI RLKTGKRAEL SYAFYGCHCG
60 70 80 90 100
LGGKGSPKDA TDRCCVTHDC CYKSLEKSGC GTKLLKYKYS HQGGQITCSA
110 120 130 140
NQNSCQKRLC QCDKAAAECF ARNKKTYSLK YQFYPNMFCK GKKPKC
Length:146
Mass (Da):16,145
Last modified:June 16, 2003 - v3
Checksum:iAB904F6B3B1BA5C7
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R1F8S4R1F8_MOUSE
Phospholipase A(2)
Pla2g2a
145Annotation score:

Sequence cautioni

The sequence AAB06315 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19V → D in CAA52325 (Ref. 1) Curated1
Sequence conflicti86K → T in CAA52325 (Ref. 1) Curated1

Polymorphismi

In strains 129/Sv, B10.RIII and C57BL/6, a polymorphism causes a frameshift and premature truncation of the protein, rendering it inactive. Strains BALB/c, C3H/He, DBA/1, DBA/2, MRL and NZB/B1N contain the normal protein while strain CD-1 is heterozygous for the mutation.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74266 mRNA Translation: CAA52325.1
U32358 Genomic DNA Translation: AAC52252.1
U28244 mRNA Translation: AAB06315.1 Different initiation.
BC045156 mRNA Translation: AAH45156.1
PIRiI48342
S29495
RefSeqiNP_001076000.1, NM_001082531.1
UniGeneiMm.4675

Genome annotation databases

GeneIDi18780
KEGGimmu:18780
UCSCiuc008vlk.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPA2GA_MOUSE
AccessioniPrimary (citable) accession number: P31482
Secondary accession number(s): Q60871
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 16, 2003
Last modified: October 25, 2017
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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