UniProtKB - P31474 (HSRA_ECOLI)
Protein
Probable transport protein HsrA
Gene
hsrA
Organism
Escherichia coli (strain K12)
Status
Functioni
Miscellaneous
Overexpression causes homocysteine accumulation and reverses rspA-mediated suppression of rpoS.1 Publication
GO - Molecular functioni
- transmembrane transporter activity Source: GO_Central
GO - Biological processi
- transmembrane transport Source: GO_Central
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:YIEO-MONOMER |
Protein family/group databases
TCDBi | 2.A.1.3.51, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Probable transport protein HsrAAlternative name(s): High-copy suppressor of rspA |
Gene namesi | Name:hsrA1 Publication Synonyms:yieO Ordered Locus Names:b3754, JW3733 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 10 – 30 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 31 – 49 | PeriplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 50 – 70 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 71 – 77 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 78 – 98 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 99 – 119 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 120 – 138 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 139 – 159 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 160 | PeriplasmicSequence analysis | 1 | |
Transmembranei | 161 – 181 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 182 – 197 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 198 – 218 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 219 – 221 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 222 – 242 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 243 – 280 | CytoplasmicSequence analysisAdd BLAST | 38 | |
Transmembranei | 281 – 301 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 302 – 322 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 323 – 328 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 329 – 349 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 350 – 370 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 371 – 393 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 394 – 414 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 415 – 428 | PeriplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 429 – 449 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 450 – 475 | CytoplasmicSequence analysis1 PublicationAdd BLAST | 26 |
GO - Cellular componenti
- integral component of membrane Source: GO_Central
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173409 | 1 – 475 | Probable transport protein HsrAAdd BLAST | 475 |
Proteomic databases
PaxDbi | P31474 |
PRIDEi | P31474 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262603, 137 interactors |
DIPi | DIP-12470N |
IntActi | P31474, 1 interactor |
STRINGi | 511145.b3754 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0477, Bacteria |
HOGENOMi | CLU_000960_28_0_6 |
InParanoidi | P31474 |
PhylomeDBi | P31474 |
Family and domain databases
InterProi | View protein in InterPro IPR004638, EmrB-like IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00711, efflux_EmrB, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P31474-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDKKKRSMA GLPWIAAMAF FMQALDATIL NTALPAIAHS LNRSPLAMQS
60 70 80 90 100
AIISYTLTVA MLIPVSGWLA DRFGTRRIFT LAVSLFTLGS LACALSNSLP
110 120 130 140 150
QLVVFRVIQG IGGAMMMPVA RLALLRAYPR NELLPVLNFV AMPGLVGPIL
160 170 180 190 200
GPVLGGVLVT WATWHWIFLI NIPIGIAGLL YARKHMPNFT TARRRFDITG
210 220 230 240 250
FLLFGLSLVL FSSGIELFGE KIVASWIALT VIVTSIGLLL LYILHARRTP
260 270 280 290 300
NPLISLDLFK TRTFSIGIVG NIATRLGTGC VPFLMPLMLQ VGFGYQAFIA
310 320 330 340 350
GCMMAPTALG SIIAKSMVTQ VLRRLGYRHT LVGITVIIGL MIAQFSLQSP
360 370 380 390 400
AMAIWMLILP LFILGMAMST QFTAMNTITL ADLTDDNASS GNSVLAVTQQ
410 420 430 440 450
LSISLGVAVS AAVLRVYEGM EGTTTVEQFH YTFITMGIIT VASAAMFMLL
460 470
KTTDGNNLIK RQRKSKPNRV PSESE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10328 Genomic DNA Translation: AAA62107.1 U00096 Genomic DNA Translation: AAC76777.1 AP009048 Genomic DNA Translation: BAE77534.1 |
PIRi | C65179 |
RefSeqi | NP_418210.1, NC_000913.3 WP_001280852.1, NZ_SSUV01000078.1 |
Genome annotation databases
EnsemblBacteriai | AAC76777; AAC76777; b3754 BAE77534; BAE77534; BAE77534 |
GeneIDi | 948265 |
KEGGi | ecj:JW3733 eco:b3754 |
PATRICi | fig|1411691.4.peg.2946 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10328 Genomic DNA Translation: AAA62107.1 U00096 Genomic DNA Translation: AAC76777.1 AP009048 Genomic DNA Translation: BAE77534.1 |
PIRi | C65179 |
RefSeqi | NP_418210.1, NC_000913.3 WP_001280852.1, NZ_SSUV01000078.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262603, 137 interactors |
DIPi | DIP-12470N |
IntActi | P31474, 1 interactor |
STRINGi | 511145.b3754 |
Protein family/group databases
TCDBi | 2.A.1.3.51, the major facilitator superfamily (mfs) |
Proteomic databases
PaxDbi | P31474 |
PRIDEi | P31474 |
Genome annotation databases
EnsemblBacteriai | AAC76777; AAC76777; b3754 BAE77534; BAE77534; BAE77534 |
GeneIDi | 948265 |
KEGGi | ecj:JW3733 eco:b3754 |
PATRICi | fig|1411691.4.peg.2946 |
Organism-specific databases
EchoBASEi | EB1683 |
Phylogenomic databases
eggNOGi | COG0477, Bacteria |
HOGENOMi | CLU_000960_28_0_6 |
InParanoidi | P31474 |
PhylomeDBi | P31474 |
Enzyme and pathway databases
BioCyci | EcoCyc:YIEO-MONOMER |
Miscellaneous databases
PROi | PR:P31474 |
Family and domain databases
InterProi | View protein in InterPro IPR004638, EmrB-like IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00711, efflux_EmrB, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HSRA_ECOLI | |
Accessioni | P31474Primary (citable) accession number: P31474 Secondary accession number(s): Q2M872 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families