UniProtKB - P31473 (RAVA_ECOLI)
Protein
ATPase RavA
Gene
ravA
Organism
Escherichia coli (strain K12)
Status
Functioni
Functions as an ATPase. May play a role in metal insertion (metal-chelatase) or as a chaperone.
Activity regulationi
The complex formed with CadA represents a possible means of regulating RavA activity in response to acid stress conditions. This interaction results in an increase in RavA ATPase activity.
Kineticsi
RavA also has GTPase activity.
- KM=790 µM for ATP (at pH 7.5)
pH dependencei
Optimum pH is 7.5.
GO - Molecular functioni
- ATPase activity Source: EcoCyc
- ATPase-coupled transmembrane transporter activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
Keywordsi
Molecular function | Hydrolase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11731-MONOMER MetaCyc:EG11731-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ravA Synonyms:yieN Ordered Locus Names:b3746, JW3725 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209370 | 1 – 498 | ATPase RavAAdd BLAST | 498 |
Proteomic databases
jPOSTi | P31473 |
PaxDbi | P31473 |
PRIDEi | P31473 |
Expressioni
Inductioni
Expression is sigma S-dependent.
Interactioni
Subunit structurei
Hexameric oligomer. Interactions of five RavA oligomers with two CadA decamers. Possible formation of a ternary complex RavA-ViaA-CadA.
1 PublicationBinary interactionsi
Hide detailsP31473
With | #Exp. | IntAct |
---|---|---|
cadA [P0A9H3] | 13 | EBI-561223,EBI-545922 |
itself | 7 | EBI-561223,EBI-561223 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262601, 626 interactors |
DIPi | DIP-12469N |
IntActi | P31473, 22 interactors |
STRINGi | 511145.b3746 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P31473 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P31473 |
Family & Domainsi
Sequence similaritiesi
Belongs to the RavA family.Curated
Phylogenomic databases
eggNOGi | COG0714, Bacteria |
HOGENOMi | CLU_018678_1_0_6 |
InParanoidi | P31473 |
PhylomeDBi | P31473 |
Family and domain databases
HAMAPi | MF_01625, ATPase_RavA, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011704, ATPase_dyneun-rel_AAA IPR023671, ATPase_RavA IPR022547, ATPase_RavA_C IPR027417, P-loop_NTPase IPR041538, RavA-like_AAA_lid |
Pfami | View protein in Pfam PF07728, AAA_5, 1 hit PF17868, AAA_lid_8, 1 hit PF12592, DUF3763, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
i Sequence
Sequence statusi: Complete.
P31473-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAHPHLLAER ISRLSSSLEK GLYERSHAIR LCLLAALSGE SVFLLGPPGI
60 70 80 90 100
AKSLIARRLK FAFQNARAFE YLMTRFSTPE EVFGPLSIQA LKDEGRYERL
110 120 130 140 150
TSGYLPEAEI VFLDEIWKAG PAILNTLLTA INERQFRNGA HVEKIPMRLL
160 170 180 190 200
VAASNELPEA DSSLEALYDR MLIRLWLDKV QDKANFRSML TSQQDENDNP
210 220 230 240 250
VPDALQVTDE EYERWQKEIG EITLPDHVFE LIFMLRQQLD KLPDAPYVSD
260 270 280 290 300
RRWKKAIRLL QASAFFSGRS AVAPVDLILL KDCLWYDAQS LNLIQQQIDV
310 320 330 340 350
LMTGHAWQQQ GMLTRLGAIV QRHLQLQQQQ SDKTALTVIR LGGIFSRRQQ
360 370 380 390 400
YQLPVNVTAS TLTLLLQKPL KLHDMEVVHI SFERSALEQW LSKGGEIRGK
410 420 430 440 450
LNGIGFAQKL NLEVDSAQHL VVRDVSLQGS TLALPGSSAE GLPGEIKQQL
460 470 480 490
EELESDWRKQ HALFSEQQKC LFIPGDWLGR IEASLQDVGA QIRQAQQC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10328 Genomic DNA Translation: AAA62099.1 Frameshift. U00096 Genomic DNA Translation: AAC76769.2 AP009048 Genomic DNA Translation: BAE77542.1 |
PIRi | C65178 |
RefSeqi | NP_418202.4, NC_000913.3 WP_001299914.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76769; AAC76769; b3746 BAE77542; BAE77542; BAE77542 |
GeneIDi | 57729064 948256 |
KEGGi | ecj:JW3725 eco:b3746 |
PATRICi | fig|511145.12.peg.3871 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10328 Genomic DNA Translation: AAA62099.1 Frameshift. U00096 Genomic DNA Translation: AAC76769.2 AP009048 Genomic DNA Translation: BAE77542.1 |
PIRi | C65178 |
RefSeqi | NP_418202.4, NC_000913.3 WP_001299914.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3NBX | X-ray | 2.91 | X | 1-498 | [»] | |
4UPB | electron microscopy | 11.00 | C/D/E | 1-498 | [»] | |
4UPF | electron microscopy | 7.50 | D | 329-440 | [»] | |
5FL2 | electron microscopy | 6.20 | K | 332-437 | [»] | |
6Q7L | electron microscopy | 7.60 | U | 1-498 | [»] | |
6SZA | electron microscopy | 6.00 | A/B/C/D/E/F | 1-498 | [»] | |
6SZB | electron microscopy | 7.00 | A/B/C/D/E/F | 1-498 | [»] | |
SMRi | P31473 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262601, 626 interactors |
DIPi | DIP-12469N |
IntActi | P31473, 22 interactors |
STRINGi | 511145.b3746 |
Proteomic databases
jPOSTi | P31473 |
PaxDbi | P31473 |
PRIDEi | P31473 |
Genome annotation databases
EnsemblBacteriai | AAC76769; AAC76769; b3746 BAE77542; BAE77542; BAE77542 |
GeneIDi | 57729064 948256 |
KEGGi | ecj:JW3725 eco:b3746 |
PATRICi | fig|511145.12.peg.3871 |
Organism-specific databases
EchoBASEi | EB1682 |
Phylogenomic databases
eggNOGi | COG0714, Bacteria |
HOGENOMi | CLU_018678_1_0_6 |
InParanoidi | P31473 |
PhylomeDBi | P31473 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11731-MONOMER MetaCyc:EG11731-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P31473 |
PROi | PR:P31473 |
Family and domain databases
HAMAPi | MF_01625, ATPase_RavA, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011704, ATPase_dyneun-rel_AAA IPR023671, ATPase_RavA IPR022547, ATPase_RavA_C IPR027417, P-loop_NTPase IPR041538, RavA-like_AAA_lid |
Pfami | View protein in Pfam PF07728, AAA_5, 1 hit PF17868, AAA_lid_8, 1 hit PF12592, DUF3763, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAVA_ECOLI | |
Accessioni | P31473Primary (citable) accession number: P31473 Secondary accession number(s): Q2M864 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 15, 1998 | |
Last modified: | April 7, 2021 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families