UniProtKB - P31466 (ADEP_ECOLI)
Protein
Adenine permease AdeP
Gene
adeP
Organism
Escherichia coli (strain K12)
Status
Functioni
High-affinity transporter for adenine.3 Publications
Activity regulationi
Internal adenine may inhibit transport.1 Publication
Kineticsi
- KM=1.0 µM for adenine1 Publication
- Vmax=1.7 nmol/min/mg enzyme1 Publication
GO - Molecular functioni
- adenine transmembrane transporter activity Source: EcoCyc
- purine nucleobase transmembrane transporter activity Source: GO_Central
- solute:proton symporter activity Source: EcoCyc
GO - Biological processi
- adenine transport Source: EcoCyc
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11724-MONOMER ECOL316407:JW3692-MONOMER MetaCyc:EG11724-MONOMER |
Protein family/group databases
TCDBi | 2.A.40.7.6 the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family |
Names & Taxonomyi
Protein namesi | Recommended name: Adenine permease AdeP |
Gene namesi | Name:adeP Synonyms:purP, yieG Ordered Locus Names:b3714, JW3692 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 2 Publications; Multi-pass membrane protein 2 Publications
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 28 | CytoplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 29 – 52 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 53 – 62 | PeriplasmicSequence analysis | 10 | |
Transmembranei | 63 – 81 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 82 – 83 | CytoplasmicSequence analysis | 2 | |
Transmembranei | 84 – 100 | Discontinuously helicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 101 – 112 | PeriplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 113 – 132 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 133 – 144 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 145 – 165 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 166 – 181 | PeriplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 182 – 199 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 200 – 203 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 204 – 222 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 223 – 250 | PeriplasmicSequence analysisAdd BLAST | 28 | |
Transmembranei | 251 – 279 | HelicalSequence analysisAdd BLAST | 29 | |
Topological domaini | 280 – 292 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 293 – 308 | HelicalSequence analysisAdd BLAST | 16 | |
Topological domaini | 309 – 310 | PeriplasmicSequence analysis | 2 | |
Transmembranei | 311 – 326 | Discontinuously helicalSequence analysisAdd BLAST | 16 | |
Topological domaini | 327 – 330 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 331 – 345 | HelicalSequence analysisAdd BLAST | 15 | |
Topological domaini | 346 – 356 | PeriplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 357 – 376 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 377 – 381 | CytoplasmicSequence analysis | 5 | |
Intramembranei | 382 – 417 | Discontinuously helicalSequence analysisAdd BLAST | 36 | |
Topological domaini | 418 – 445 | CytoplasmicSequence analysisAdd BLAST | 28 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 38 | T → A: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 38 | T → H: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 91 | A → G or S: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 267 | D → A or N: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 267 | D → E: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 271 | T → A or S: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 271 | T → D: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 271 | T → N: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 298 | D → E: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 298 | D → N: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 317 | I → A: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 317 | I → E: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 318 | E → D: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 318 | E → Q: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 319 | S → A or N: Almost no change in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169638 | 1 – 445 | Adenine permease AdePAdd BLAST | 445 |
Proteomic databases
jPOSTi | P31466 |
PaxDbi | P31466 |
PRIDEi | P31466 |
Interactioni
Protein-protein interaction databases
BioGridi | 4262172, 283 interactors |
STRINGi | 511145.b3714 |
Family & Domainsi
Sequence similaritiesi
Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG4105C7N Bacteria COG2252 LUCA |
HOGENOMi | HOG000244362 |
InParanoidi | P31466 |
KOi | K06901 |
PhylomeDBi | P31466 |
Family and domain databases
InterProi | View protein in InterPro IPR026033 Azg-like IPR006043 Xant/urac/vitC |
Pfami | View protein in Pfam PF00860 Xan_ur_permease, 1 hit |
PIRSFi | PIRSF005353 PbuG, 1 hit |
i Sequence
Sequence statusi: Complete.
P31466-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSHQHTTQTS GQGMLERVFK LREHGTTART EVIAGFTTFL TMVYIVFVNP
60 70 80 90 100
QILGVAGMDT SAVFVTTCLI AAFGSIMMGL FANLPVALAP AMGLNAFFAF
110 120 130 140 150
VVVQAMGLPW QVGMGAIFWG AIGLLLLTIF RVRYWMIANI PVSLRVGITS
160 170 180 190 200
GIGLFIGMMG LKNAGVIVAN PETLVSIGNL TSHSVLLGIL GFFIIAILAS
210 220 230 240 250
RNIHAAVLVS IVVTTLLGWM LGDVHYNGIV SAPPSVMTVV GHVDLAGSFN
260 270 280 290 300
LGLAGVIFSF MLVNLFDSSG TLIGVTDKAG LADEKGKFPR MKQALYVDSI
310 320 330 340 350
SSVTGSFIGT SSVTAYIESS SGVSVGGRTG LTAVVVGLLF LLVIFLSPLA
360 370 380 390 400
GMVPGYAAAG ALIYVGVLMT SSLARVNWQD LTESVPAFIT AVMMPFSFSI
410 420 430 440
TEGIALGFIS YCVMKIGTGR LRDLSPCVII VALLFILKIV FIDAH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10328 Genomic DNA Translation: AAA62065.1 U00096 Genomic DNA Translation: AAC76737.1 AP009048 Genomic DNA Translation: BAE77574.1 |
PIRi | C65174 |
RefSeqi | NP_418170.1, NC_000913.3 WP_000019348.1, NZ_SSZK01000035.1 |
Genome annotation databases
EnsemblBacteriai | AAC76737; AAC76737; b3714 BAE77574; BAE77574; BAE77574 |
GeneIDi | 948224 |
KEGGi | ecj:JW3692 eco:b3714 |
PATRICi | fig|1411691.4.peg.2987 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10328 Genomic DNA Translation: AAA62065.1 U00096 Genomic DNA Translation: AAC76737.1 AP009048 Genomic DNA Translation: BAE77574.1 |
PIRi | C65174 |
RefSeqi | NP_418170.1, NC_000913.3 WP_000019348.1, NZ_SSZK01000035.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGridi | 4262172, 283 interactors |
STRINGi | 511145.b3714 |
Protein family/group databases
TCDBi | 2.A.40.7.6 the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family |
Proteomic databases
jPOSTi | P31466 |
PaxDbi | P31466 |
PRIDEi | P31466 |
Protocols and materials databases
DNASUi | 948224 |
Genome annotation databases
EnsemblBacteriai | AAC76737; AAC76737; b3714 BAE77574; BAE77574; BAE77574 |
GeneIDi | 948224 |
KEGGi | ecj:JW3692 eco:b3714 |
PATRICi | fig|1411691.4.peg.2987 |
Organism-specific databases
EchoBASEi | EB1675 |
Phylogenomic databases
eggNOGi | ENOG4105C7N Bacteria COG2252 LUCA |
HOGENOMi | HOG000244362 |
InParanoidi | P31466 |
KOi | K06901 |
PhylomeDBi | P31466 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11724-MONOMER ECOL316407:JW3692-MONOMER MetaCyc:EG11724-MONOMER |
Miscellaneous databases
PROi | PR:P31466 |
Family and domain databases
InterProi | View protein in InterPro IPR026033 Azg-like IPR006043 Xant/urac/vitC |
Pfami | View protein in Pfam PF00860 Xan_ur_permease, 1 hit |
PIRSFi | PIRSF005353 PbuG, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADEP_ECOLI | |
Accessioni | P31466Primary (citable) accession number: P31466 Secondary accession number(s): P76741, Q2M832 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | November 1, 1997 | |
Last modified: | November 13, 2019 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene