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UniProtKB - P31466 (ADEP_ECOLI)

Entry version 143 (02 Dec 2020)
Sequence version 2 (01 Nov 1997)
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Protein

Adenine permease AdeP

Gene

adeP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

High-affinity transporter for adenine.3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Internal adenine may inhibit transport.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=1.0 µM for adenine1 Publication
  1. Vmax=1.7 nmol/min/mg enzyme1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EG11724-MONOMER
MetaCyc:EG11724-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.40.7.6, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Adenine permease AdeP
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:adeP
Synonyms:purP, yieG
Ordered Locus Names:b3714, JW3692
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 28CytoplasmicSequence analysisAdd BLAST28
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei29 – 52HelicalSequence analysisAdd BLAST24
Topological domaini53 – 62PeriplasmicSequence analysis10
Transmembranei63 – 81HelicalSequence analysisAdd BLAST19
Topological domaini82 – 83CytoplasmicSequence analysis2
Transmembranei84 – 100Discontinuously helicalSequence analysisAdd BLAST17
Topological domaini101 – 112PeriplasmicSequence analysisAdd BLAST12
Transmembranei113 – 132HelicalSequence analysisAdd BLAST20
Topological domaini133 – 144CytoplasmicSequence analysisAdd BLAST12
Transmembranei145 – 165HelicalSequence analysisAdd BLAST21
Topological domaini166 – 181PeriplasmicSequence analysisAdd BLAST16
Transmembranei182 – 199HelicalSequence analysisAdd BLAST18
Topological domaini200 – 203CytoplasmicSequence analysis4
Transmembranei204 – 222HelicalSequence analysisAdd BLAST19
Topological domaini223 – 250PeriplasmicSequence analysisAdd BLAST28
Transmembranei251 – 279HelicalSequence analysisAdd BLAST29
Topological domaini280 – 292CytoplasmicSequence analysisAdd BLAST13
Transmembranei293 – 308HelicalSequence analysisAdd BLAST16
Topological domaini309 – 310PeriplasmicSequence analysis2
Transmembranei311 – 326Discontinuously helicalSequence analysisAdd BLAST16
Topological domaini327 – 330CytoplasmicSequence analysis4
Transmembranei331 – 345HelicalSequence analysisAdd BLAST15
Topological domaini346 – 356PeriplasmicSequence analysisAdd BLAST11
Transmembranei357 – 376HelicalSequence analysisAdd BLAST20
Topological domaini377 – 381CytoplasmicSequence analysis5
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a region that is buried within a membrane, but does not cross it.<p><a href='/help/intramem' target='_top'>More...</a></p>Intramembranei382 – 417Discontinuously helicalSequence analysisAdd BLAST36
Topological domaini418 – 445CytoplasmicSequence analysisAdd BLAST28

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi38T → A: Almost no change in activity. 1 Publication1
Mutagenesisi38T → H: Lack of activity. 1 Publication1
Mutagenesisi91A → G or S: Almost no change in activity. 1 Publication1
Mutagenesisi267D → A or N: Lack of activity. 1 Publication1
Mutagenesisi267D → E: Strong decrease in activity. 1 Publication1
Mutagenesisi271T → A or S: Almost no change in activity. 1 Publication1
Mutagenesisi271T → D: Lack of activity. 1 Publication1
Mutagenesisi271T → N: Strong decrease in activity. 1 Publication1
Mutagenesisi298D → E: Almost no change in activity. 1 Publication1
Mutagenesisi298D → N: Lack of activity. 1 Publication1
Mutagenesisi317I → A: Almost no change in activity. 1 Publication1
Mutagenesisi317I → E: Strong decrease in activity. 1 Publication1
Mutagenesisi318E → D: Strong decrease in activity. 1 Publication1
Mutagenesisi318E → Q: Lack of activity. 1 Publication1
Mutagenesisi319S → A or N: Almost no change in activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001696381 – 445Adenine permease AdePAdd BLAST445

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P31466

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P31466

PRoteomics IDEntifications database

More...PRIDEi		P31466

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4262172, 283 interactors

STRING: functional protein association networks

More...STRINGi		511145.b3714

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily. [View classification]Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG2252, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_024508_0_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P31466

Database for complete collections of gene phylogenies

More...PhylomeDBi		P31466

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR026033, Azg-like
IPR006043, Xant/urac/vitC

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00860, Xan_ur_permease, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF005353, PbuG, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P31466-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSHQHTTQTS GQGMLERVFK LREHGTTART EVIAGFTTFL TMVYIVFVNP 
        60         70         80         90        100
QILGVAGMDT SAVFVTTCLI AAFGSIMMGL FANLPVALAP AMGLNAFFAF 
       110        120        130        140        150
VVVQAMGLPW QVGMGAIFWG AIGLLLLTIF RVRYWMIANI PVSLRVGITS 
       160        170        180        190        200
GIGLFIGMMG LKNAGVIVAN PETLVSIGNL TSHSVLLGIL GFFIIAILAS 
       210        220        230        240        250
RNIHAAVLVS IVVTTLLGWM LGDVHYNGIV SAPPSVMTVV GHVDLAGSFN 
       260        270        280        290        300
LGLAGVIFSF MLVNLFDSSG TLIGVTDKAG LADEKGKFPR MKQALYVDSI 
       310        320        330        340        350
SSVTGSFIGT SSVTAYIESS SGVSVGGRTG LTAVVVGLLF LLVIFLSPLA 
       360        370        380        390        400
GMVPGYAAAG ALIYVGVLMT SSLARVNWQD LTESVPAFIT AVMMPFSFSI 
       410        420        430        440 
TEGIALGFIS YCVMKIGTGR LRDLSPCVII VALLFILKIV FIDAH
Length:445
Mass (Da):46,866
Last modified:November 1, 1997 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i899FC4A38FD62E2A
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
L10328 Genomic DNA Translation: AAA62065.1
U00096 Genomic DNA Translation: AAC76737.1
AP009048 Genomic DNA Translation: BAE77574.1

Protein sequence database of the Protein Information Resource

More...PIRi		C65174

NCBI Reference Sequences

More...RefSeqi		NP_418170.1, NC_000913.3
WP_000019348.1, NZ_SSZK01000035.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC76737; AAC76737; b3714
BAE77574; BAE77574; BAE77574

Database of genes from NCBI RefSeq genomes

More...GeneIDi		948224

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW3692
eco:b3714

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.2987

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA Translation: AAA62065.1
U00096 Genomic DNA Translation: AAC76737.1
AP009048 Genomic DNA Translation: BAE77574.1
PIRiC65174
RefSeqiNP_418170.1, NC_000913.3
WP_000019348.1, NZ_SSZK01000035.1

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi4262172, 283 interactors
STRINGi511145.b3714

Protein family/group databases

TCDBi2.A.40.7.6, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family

Proteomic databases

jPOSTiP31466
PaxDbiP31466
PRIDEiP31466

Protocols and materials databases

The DNASU plasmid repository

More...DNASUi		948224

Genome annotation databases

EnsemblBacteriaiAAC76737; AAC76737; b3714
BAE77574; BAE77574; BAE77574
GeneIDi948224
KEGGiecj:JW3692
eco:b3714
PATRICifig|1411691.4.peg.2987

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB1675

Phylogenomic databases

eggNOGiCOG2252, Bacteria
HOGENOMiCLU_024508_0_1_6
InParanoidiP31466
PhylomeDBiP31466

Enzyme and pathway databases

BioCyciEcoCyc:EG11724-MONOMER
MetaCyc:EG11724-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P31466

Family and domain databases

InterProiView protein in InterPro
IPR026033, Azg-like
IPR006043, Xant/urac/vitC
PfamiView protein in Pfam
PF00860, Xan_ur_permease, 1 hit
PIRSFiPIRSF005353, PbuG, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiADEP_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P31466
Secondary accession number(s): P76741, Q2M832
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: December 2, 2020
This is version 143 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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