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Protein

Adenine deaminase

Gene

ade

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Ade is a cryptic gene in wild-type strains. The physiological conditions that lead to its activation are unknown.

Catalytic activityi

Adenine + H2O = hypoxanthine + NH3.2 Publications

Cofactori

Mn2+2 Publications

pH dependencei

Optimum pH is 6.5-7.0.

Temperature dependencei

Optimum temperature is 60 degrees Celsius.

GO - Molecular functioni

  • adenine deaminase activity Source: EcoCyc
  • catalase activity Source: EcoCyc
  • ferrous iron binding Source: EcoCyc
  • manganese ion binding Source: EcoCyc
  • N-acetylglucosamine-6-phosphate deacetylase activity Source: GO_Central

GO - Biological processi

  • adenine catabolic process Source: EcoliWiki
  • hypoxanthine biosynthetic process Source: EcoliWiki
  • N-acetylglucosamine catabolic process Source: GO_Central

Keywordsi

Molecular functionHydrolase
LigandManganese

Enzyme and pathway databases

BioCyciEcoCyc:EG11692-MONOMER
MetaCyc:EG11692-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine deaminase (EC:3.5.4.2)
Short name:
Adenase
Short name:
Adenine aminase
Gene namesi
Name:ade
Synonyms:yicP
Ordered Locus Names:b3665, JW3640
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11692 ade

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001424201 – 588Adenine deaminaseAdd BLAST588

Proteomic databases

PaxDbiP31441
PRIDEiP31441

2D gel databases

SWISS-2DPAGEiP31441

Expressioni

Inductioni

Repressed by H-NS. Activated by insertion of a variety of IS elements into a region extending from -145 to +13 relative to the transcription start site. IS elements essentially eliminate the H-NS-mediated silencing, but also stimulate ade expression 2-3 fold independently of the H-NS protein.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4262580, 9 interactors
DIPiDIP-12439N
IntActiP31441, 11 interactors
STRINGi316385.ECDH10B_3848

Structurei

3D structure databases

ProteinModelPortaliP31441
SMRiP31441
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CPN Bacteria
COG1001 LUCA
HOGENOMiHOG000276949
InParanoidiP31441
KOiK01486
OMAiHEIANVM
PhylomeDBiP31441

Family and domain databases

CDDicd01295 AdeC, 1 hit
Gene3Di2.30.40.10, 2 hits
HAMAPiMF_01518 Adenine_deamin, 1 hit
InterProiView protein in InterPro
IPR006679 Adenine_deam
IPR026912 Adenine_deam_C
IPR006680 Amidohydro-rel
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
PfamiView protein in Pfam
PF13382 Adenine_deam_C, 1 hit
PF01979 Amidohydro_1, 1 hit
SUPFAMiSSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR01178 ade, 1 hit

Sequencei

Sequence statusi: Complete.

P31441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSINHKFH HISRAEYQEL LAVSRGDAVA DYIIDNVSIL DLINGGEISG
60 70 80 90 100
PIVIKGRYIA GVGAEYTDAP ALQRIDARGA TAVPGFIDAH LHIESSMMTP
110 120 130 140 150
VTFETATLPR GLTTVICDPH EIVNVMGEAG FAWFARCAEQ ARQNQYLQVS
160 170 180 190 200
SCVPALEGCD VNGASFTLEQ MLAWRDHPQV TGLAEMMDYP GVISGQNALL
210 220 230 240 250
DKLDAFRHLT LDGHCPGLGG KELNAYITAG IENCHESYQL EEGRRKLQLG
260 270 280 290 300
MSLMIREGSA ARNLNALAPL INEFNSPQCM LCTDDRNPWE IAHEGHIDAL
310 320 330 340 350
IRRLIEQHNV PLHVAYRVAS WSTARHFGLN HLGLLAPGKQ ADIVLLSDAR
360 370 380 390 400
KVTVQQVLVK GEPIDAQTLQ AEESARLAQS APPYGNTIAR QPVSASDFAL
410 420 430 440 450
QFTPGKRYRV IDVIHNELIT HSHSSVYSEN GFDRDDVSFI AVLERYGQRL
460 470 480 490 500
APACGLLGGF GLNEGALAAT VSHDSHNIVV IGRSAEEMAL AVNQVIQDGG
510 520 530 540 550
GLCVVRNGQV QSHLPLPIAG LMSTDTAQSL AEQIDALKAA ARECGPLPDE
560 570 580
PFIQMAFLSL PVIPALKLTS QGLFDGEKFA FTTLEVTE
Length:588
Mass (Da):63,739
Last modified:November 1, 1997 - v2
Checksum:i2732C6B1B6595E48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA Translation: AAA62017.1
U00096 Genomic DNA Translation: AAC76688.1
AP009048 Genomic DNA Translation: BAE77628.1
PIRiB65168
RefSeqiNP_418121.1, NC_000913.3
WP_001065718.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76688; AAC76688; b3665
BAE77628; BAE77628; BAE77628
GeneIDi948210
KEGGiecj:JW3640
eco:b3665
PATRICifig|1411691.4.peg.3040

Similar proteinsi

Entry informationi

Entry nameiADEC_ECOLI
AccessioniPrimary (citable) accession number: P31441
Secondary accession number(s): P78121, Q2M7X8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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