UniProtKB - P31424 (GRM5_RAT)
Metabotropic glutamate receptor 5
Grm5
Functioni
Miscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 64 | GlutamateBy similarity | 1 | |
Binding sitei | 151 | GlutamateBy similarity | 1 | |
Binding sitei | 222 | GlutamateBy similarity | 1 | |
Binding sitei | 304 | GlutamateBy similarity | 1 | |
Binding sitei | 395 | GlutamateBy similarity | 1 |
GO - Molecular functioni
- A2A adenosine receptor binding Source: RGD
- calmodulin binding Source: UniProtKB
- glutamate receptor activity Source: UniProtKB
- G protein-coupled receptor activity Source: UniProtKB
- G protein-coupled receptor activity involved in regulation of postsynaptic membrane potential Source: RGD
- identical protein binding Source: RGD
- neurotransmitter receptor activity involved in regulation of postsynaptic cytosolic calcium ion concentration Source: RGD
- PDZ domain binding Source: UniProtKB
- PLC activating G protein-coupled glutamate receptor activity Source: UniProtKB
- protein tyrosine kinase binding Source: ARUK-UCL
GO - Biological processi
- activation of MAPKKK activity Source: UniProtKB
- calcium-mediated signaling using intracellular calcium source Source: RGD
- cellular response to amyloid-beta Source: RGD
- chemical synaptic transmission Source: RGD
- cognition Source: RGD
- desensitization of G protein-coupled receptor signaling pathway Source: UniProtKB
- G protein-coupled glutamate receptor signaling pathway Source: UniProtKB
- learning Source: RGD
- learning or memory Source: RGD
- locomotory behavior Source: RGD
- modulation of age-related behavioral decline Source: RGD
- negative regulation of locomotion Source: RGD
- phosphatidylinositol-mediated signaling Source: UniProtKB
- phospholipase C-activating G protein-coupled glutamate receptor signaling pathway Source: UniProtKB
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G protein-coupled signaling pathway Source: UniProtKB
- positive regulation of long-term neuronal synaptic plasticity Source: RGD
- positive regulation of protein tyrosine kinase activity Source: ARUK-UCL
- postsynaptic modulation of chemical synaptic transmission Source: RGD
- protein kinase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of intracellular calcium activated chloride channel activity Source: RGD
- regulation of long-term neuronal synaptic plasticity Source: RGD
- regulation of protein phosphorylation Source: RGD
- regulation of synaptic transmission, glutamatergic Source: GO_Central
- regulation of transcription, DNA-templated Source: RGD
- regulation of translational elongation Source: RGD
- synapse organization Source: RGD
- trans-synaptic signaling by endocannabinoid, modulating synaptic transmission Source: SynGO
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-416476, G alpha (q) signalling events R-RNO-420499, Class C/3 (Metabotropic glutamate/pheromone receptors) R-RNO-6794361, Neurexins and neuroligins |
Names & Taxonomyi
Protein namesi | Recommended name: Metabotropic glutamate receptor 5Short name: mGluR5 |
Gene namesi | Name:Grm5 Synonyms:Gprc1e, Mglur5 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2746, Grm5 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- integral component of postsynaptic membrane Source: SynGO
- plasma membrane Source: RGD
- postsynaptic density membrane Source: SynGO
- postsynaptic membrane Source: UniProtKB
Other locations
- astrocyte projection Source: RGD
- cytoplasm Source: RGD
- dendritic shaft Source: RGD
- dendritic spine Source: RGD
- glutamatergic synapse Source: SynGO
- membrane Source: RGD
- neuron projection Source: RGD
- postsynaptic density Source: RGD
- Schaffer collateral - CA1 synapse Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 579 | ExtracellularBy similarityAdd BLAST | 559 | |
Transmembranei | 580 – 602 | Helical; Name=1By similarityAdd BLAST | 23 | |
Topological domaini | 603 – 612 | CytoplasmicBy similarity | 10 | |
Transmembranei | 613 – 635 | Helical; Name=2By similarityAdd BLAST | 23 | |
Topological domaini | 636 – 643 | ExtracellularBy similarity | 8 | |
Transmembranei | 644 – 666 | Helical; Name=3By similarityAdd BLAST | 23 | |
Topological domaini | 667 – 692 | CytoplasmicBy similarityAdd BLAST | 26 | |
Transmembranei | 693 – 713 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 714 – 736 | ExtracellularBy similarityAdd BLAST | 23 | |
Transmembranei | 737 – 758 | Helical; Name=5By similarityAdd BLAST | 22 | |
Topological domaini | 759 – 771 | CytoplasmicBy similarityAdd BLAST | 13 | |
Transmembranei | 772 – 794 | Helical; Name=6By similarityAdd BLAST | 23 | |
Topological domaini | 795 – 797 | ExtracellularBy similarity | 3 | |
Transmembranei | 798 – 819 | Helical; Name=7By similarityAdd BLAST | 22 | |
Topological domaini | 820 – 1203 | CytoplasmicBy similarityAdd BLAST | 384 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1154 | L → V: Normal binding to HOMER1. 1 Publication | 1 | |
Mutagenesisi | 1156 | P → K: Disrupts binding to HOMER1. 1 Publication | 1 | |
Mutagenesisi | 1157 | P → E: Disrupts binding to HOMER1. 1 Publication | 1 | |
Mutagenesisi | 1157 | P → L: Disrupts binding to HOMER1. 1 Publication | 1 | |
Mutagenesisi | 1158 | S → F: Normal binding to HOMER1. 1 Publication | 1 | |
Mutagenesisi | 1159 | P → A: Normal binding to HOMER1. 1 Publication | 1 | |
Mutagenesisi | 1160 | F → R: Disrupts binding to HOMER1. | 1 | |
Mutagenesisi | 1161 | R → T: Normal binding to HOMER1. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2564 |
DrugCentrali | P31424 |
GuidetoPHARMACOLOGYi | 293 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000012933 | 21 – 1203 | Metabotropic glutamate receptor 5Add BLAST | 1183 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 57 ↔ 99 | By similarity | ||
Glycosylationi | 88 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 209 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 240 ↔ 529 | By similarity | ||
Disulfide bondi | 275 ↔ 277 | By similarity | ||
Disulfide bondi | 364 ↔ 380 | By similarity | ||
Glycosylationi | 377 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 381 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 418 ↔ 425 | By similarity | ||
Glycosylationi | 444 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 510 ↔ 530 | By similarity | ||
Disulfide bondi | 514 ↔ 533 | By similarity | ||
Disulfide bondi | 536 ↔ 548 | By similarity | ||
Disulfide bondi | 551 ↔ 564 | By similarity | ||
Disulfide bondi | 643 ↔ 732 | By similarity | ||
Glycosylationi | 733 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 860 | PhosphoserineCombined sources | 1 | |
Modified residuei | 868 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 924 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 1014 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1016 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Methylation, PhosphoproteinProteomic databases
PaxDbi | P31424 |
PRIDEi | P31424 |
PTM databases
GlyGeni | P31424, 6 sites |
iPTMneti | P31424 |
PhosphoSitePlusi | P31424 |
Expressioni
Tissue specificityi
Gene expression databases
Genevisiblei | P31424, RN |
Interactioni
Subunit structurei
Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. The PPXXF motif binds HOMER1, HOMER2 and HOMER3.
Interacts with SIAH1 and TAMALIN.
Interacts with NCDN.
Interacts with NECAB2.
Interacts with CAMK2A (By similarity).
By similarity6 PublicationsBinary interactionsi
Hide detailsP31424
With | #Exp. | IntAct |
---|---|---|
Ppp1cc [P63088] | 19 | EBI-2902734,EBI-80049 |
Tamalin [Q8R4T5] | 5 | EBI-2902734,EBI-7361884 |
Isoform 2 [P31424-1]
With | #Exp. | IntAct |
---|---|---|
ADORA2A [P29274] from Homo sapiens. | 3 | EBI-2902778,EBI-2902702 |
Isoform 1 [P31424-2]
GO - Molecular functioni
- A2A adenosine receptor binding Source: RGD
- calmodulin binding Source: UniProtKB
- identical protein binding Source: RGD
- PDZ domain binding Source: UniProtKB
- protein tyrosine kinase binding Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 246583, 11 interactors |
CORUMi | P31424 |
DIPi | DIP-41263N |
ELMi | P31424 |
IntActi | P31424, 13 interactors |
MINTi | P31424 |
STRINGi | 10116.ENSRNOP00000022059 |
Chemistry databases
BindingDBi | P31424 |
Structurei
3D structure databases
SMRi | P31424 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P31424 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 172 – 174 | Glutamate bindingBy similarity | 3 | |
Regioni | 804 – 808 | Allosteric effector bindingBy similarity | 5 |
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1056, Eukaryota |
HOGENOMi | CLU_005389_0_1_1 |
InParanoidi | P31424 |
PhylomeDBi | P31424 |
TreeFami | TF313240 |
Family and domain databases
Gene3Di | 2.10.50.30, 1 hit |
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR000337, GPCR_3 IPR011500, GPCR_3_9-Cys_dom IPR038550, GPCR_3_9-Cys_sf IPR017978, GPCR_3_C IPR017979, GPCR_3_CS IPR000162, GPCR_3_mtglu_rcpt IPR000202, GPCR_3_mtglu_rcpt_5 IPR019588, Metabotropic_Glu_rcpt_Homer-bd IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF00003, 7tm_3, 1 hit PF01094, ANF_receptor, 1 hit PF10606, GluR_Homer-bdg, 1 hit PF07562, NCD3G, 1 hit |
PRINTSi | PR00248, GPCRMGR PR01055, MTABOTROPC5R PR00593, MTABOTROPICR |
SMARTi | View protein in SMART SM01229, GluR_Homer-bdg, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
PROSITEi | View protein in PROSITE PS00979, G_PROTEIN_RECEP_F3_1, 1 hit PS00980, G_PROTEIN_RECEP_F3_2, 1 hit PS00981, G_PROTEIN_RECEP_F3_3, 1 hit PS50259, G_PROTEIN_RECEP_F3_4, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVLLLILSVL LLKEDVRGSA QSSERRVVAH MPGDIIIGAL FSVHHQPTVD
60 70 80 90 100
KVHERKCGAV REQYGIQRVE AMLHTLERIN SDPTLLPNIT LGCEIRDSCW
110 120 130 140 150
HSAVALEQSI EFIRDSLISS EEEEGLVRCV DGSSSFRSKK PIVGVIGPGS
160 170 180 190 200
SSVAIQVQNL LQLFNIPQIA YSATSMDLSD KTLFKYFMRV VPSDAQQARA
210 220 230 240 250
MVDIVKRYNW TYVSAVHTEG NYGESGMEAF KDMSAKEGIC IAHSYKIYSN
260 270 280 290 300
AGEQSFDKLL KKLRSHLPKA RVVACFCEGM TVRGLLMAMR RLGLAGEFLL
310 320 330 340 350
LGSDGWADRY DVTDGYQREA VGGITIKLQS PDVKWFDDYY LKLRPETNLR
360 370 380 390 400
NPWFQEFWQH RFQCRLEGFA QENSKYNKTC NSSLTLRTHH VQDSKMGFVI
410 420 430 440 450
NAIYSMAYGL HNMQMSLCPG YAGLCDAMKP IDGRKLLDSL MKTNFTGVSG
460 470 480 490 500
DMILFDENGD SPGRYEIMNF KEMGKDYFDY INVGSWDNGE LKMDDDEVWS
510 520 530 540 550
KKNNIIRSVC SEPCEKGQIK VIRKGEVSCC WTCTPCKENE YVFDEYTCKA
560 570 580 590 600
CQLGSWPTDD LTGCDLIPVQ YLRWGDPEPI AAVVFACLGL LATLFVTVIF
610 620 630 640 650
IIYRDTPVVK SSSRELCYII LAGICLGYLC TFCLIAKPKQ IYCYLQRIGI
660 670 680 690 700
GLSPAMSYSA LVTKTNRIAR ILAGSKKKIC TKKPRFMSAC AQLVIAFILI
710 720 730 740 750
CIQLGIIVAL FIMEPPDIMH DYPSIREVYL ICNTTNLGVV TPLGYNGLLI
760 770 780 790 800
LSCTFYAFKT RNVPANFNEA KYIAFTMYTT CIIWLAFVPI YFGSNYKIIT
810 820 830 840 850
MCFSVSLSAT VALGCMFVPK VYIILAKPER NVRSAFTTST VVRMHVGDGK
860 870 880 890 900
SSSAASRSSS LVNLWKRRGS SGETLRYKDR RLAQHKSEIE CFTPKGSMGN
910 920 930 940 950
GGRATMSSSN GKSVTWAQNE KSTRGQHLWQ RLSVHINKKE NPNQTAVIKP
960 970 980 990 1000
FPKSTENRGP GAAAGGGSGP GVAGAGNAGC TATGGPEPPD AGPKALYDVA
1010 1020 1030 1040 1050
EAEESFPAAA RPRSPSPIST LSHLAGSAGR TDDDAPSLHS ETAARSSSSQ
1060 1070 1080 1090 1100
GSLMEQISSV VTRFTANISE LNSMMLSTAA TPGPPGTPIC SSYLIPKEIQ
1110 1120 1130 1140 1150
LPTTMTTFAE IQPLPAIEVT GGAQGATGVS PAQETPTGAE SAPGKPDLEE
1160 1170 1180 1190 1200
LVALTPPSPF RDSVDSGSTT PNSPVSESAL CIPSSPKYDT LIIRDYTQSS
SSL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0H2UHN1 | A0A0H2UHN1_RAT | Metabotropic glutamate receptor 5 | Grm5 | 994 | Annotation score: | ||
A0A0H2UHW6 | A0A0H2UHW6_RAT | Metabotropic glutamate receptor 5 | Grm5 | 962 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002031 | 876 – 907 | Missing in isoform 1. 1 PublicationAdd BLAST | 32 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10891 mRNA Translation: BAA01711.1 S64315 mRNA Translation: AAB27666.1 |
PIRi | A42916 PN0549 |
RefSeqi | NP_058708.1, NM_017012.1 [P31424-2] XP_006229721.1, XM_006229659.3 [P31424-1] XP_017444267.1, XM_017588778.1 [P31424-1] XP_017444268.1, XM_017588779.1 [P31424-1] XP_017444269.1, XM_017588780.1 [P31424-1] XP_017444270.1, XM_017588781.1 [P31424-2] |
Genome annotation databases
GeneIDi | 24418 |
KEGGi | rno:24418 |
UCSCi | RGD:2746, rat [P31424-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10891 mRNA Translation: BAA01711.1 S64315 mRNA Translation: AAB27666.1 |
PIRi | A42916 PN0549 |
RefSeqi | NP_058708.1, NM_017012.1 [P31424-2] XP_006229721.1, XM_006229659.3 [P31424-1] XP_017444267.1, XM_017588778.1 [P31424-1] XP_017444268.1, XM_017588779.1 [P31424-1] XP_017444269.1, XM_017588780.1 [P31424-1] XP_017444270.1, XM_017588781.1 [P31424-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DDV | X-ray | 1.90 | B | 1155-1160 | [»] | |
SMRi | P31424 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 246583, 11 interactors |
CORUMi | P31424 |
DIPi | DIP-41263N |
ELMi | P31424 |
IntActi | P31424, 13 interactors |
MINTi | P31424 |
STRINGi | 10116.ENSRNOP00000022059 |
Chemistry databases
BindingDBi | P31424 |
ChEMBLi | CHEMBL2564 |
DrugCentrali | P31424 |
GuidetoPHARMACOLOGYi | 293 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P31424, 6 sites |
iPTMneti | P31424 |
PhosphoSitePlusi | P31424 |
Proteomic databases
PaxDbi | P31424 |
PRIDEi | P31424 |
Protocols and materials databases
ABCDi | P31424, 2 sequenced antibodies |
Genome annotation databases
GeneIDi | 24418 |
KEGGi | rno:24418 |
UCSCi | RGD:2746, rat [P31424-1] |
Organism-specific databases
CTDi | 2915 |
RGDi | 2746, Grm5 |
Phylogenomic databases
eggNOGi | KOG1056, Eukaryota |
HOGENOMi | CLU_005389_0_1_1 |
InParanoidi | P31424 |
PhylomeDBi | P31424 |
TreeFami | TF313240 |
Enzyme and pathway databases
Reactomei | R-RNO-416476, G alpha (q) signalling events R-RNO-420499, Class C/3 (Metabotropic glutamate/pheromone receptors) R-RNO-6794361, Neurexins and neuroligins |
Miscellaneous databases
EvolutionaryTracei | P31424 |
PROi | PR:P31424 |
Gene expression databases
Genevisiblei | P31424, RN |
Family and domain databases
Gene3Di | 2.10.50.30, 1 hit |
InterProi | View protein in InterPro IPR001828, ANF_lig-bd_rcpt IPR000337, GPCR_3 IPR011500, GPCR_3_9-Cys_dom IPR038550, GPCR_3_9-Cys_sf IPR017978, GPCR_3_C IPR017979, GPCR_3_CS IPR000162, GPCR_3_mtglu_rcpt IPR000202, GPCR_3_mtglu_rcpt_5 IPR019588, Metabotropic_Glu_rcpt_Homer-bd IPR028082, Peripla_BP_I |
Pfami | View protein in Pfam PF00003, 7tm_3, 1 hit PF01094, ANF_receptor, 1 hit PF10606, GluR_Homer-bdg, 1 hit PF07562, NCD3G, 1 hit |
PRINTSi | PR00248, GPCRMGR PR01055, MTABOTROPC5R PR00593, MTABOTROPICR |
SMARTi | View protein in SMART SM01229, GluR_Homer-bdg, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
PROSITEi | View protein in PROSITE PS00979, G_PROTEIN_RECEP_F3_1, 1 hit PS00980, G_PROTEIN_RECEP_F3_2, 1 hit PS00981, G_PROTEIN_RECEP_F3_3, 1 hit PS50259, G_PROTEIN_RECEP_F3_4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GRM5_RAT | |
Accessioni | P31424Primary (citable) accession number: P31424 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries