UniProtKB - P31394 (PROC_RAT)
Protein
Vitamin K-dependent protein C
Gene
Proc
Organism
Rattus norvegicus (Rat)
Status
Functioni
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.By similarity
Miscellaneous
Calcium also binds, with stronger affinity to another site, beyond the GLA domain. This GLA-independent binding site is necessary for the recognition of the thrombin-thrombomodulin complex.
Catalytic activityi
- Degradation of blood coagulation factors Va and VIIIa. EC:3.4.21.69
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 254 | Charge relay system | 1 | |
Active sitei | 300 | Charge relay system | 1 | |
Active sitei | 402 | Charge relay system | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- oligopeptidase activity Source: RGD
- peptidase activity Source: RGD
- protein self-association Source: RGD
- serine-type endopeptidase activity Source: UniProtKB
GO - Biological processi
- blood coagulation Source: UniProtKB-KW
- liver development Source: RGD
- negative regulation of apoptotic process Source: RGD
- negative regulation of blood coagulation Source: RGD
- negative regulation of coagulation Source: UniProtKB
- negative regulation of inflammatory response Source: UniProtKB
- positive regulation of establishment of endothelial barrier Source: UniProtKB
- proteolysis Source: RGD
- regulation of circulating fibrinogen levels Source: RGD
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Blood coagulation, Hemostasis |
Ligand | Calcium |
Enzyme and pathway databases
Reactomei | R-RNO-140837, Intrinsic Pathway of Fibrin Clot Formation R-RNO-140875, Common Pathway of Fibrin Clot Formation R-RNO-159740, Gamma-carboxylation of protein precursors R-RNO-159763, Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus R-RNO-159782, Removal of aminoterminal propeptides from gamma-carboxylated proteins R-RNO-202733, Cell surface interactions at the vascular wall R-RNO-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-RNO-8957275, Post-translational protein phosphorylation |
Protein family/group databases
MEROPSi | S01.218 |
Names & Taxonomyi
Protein namesi | Recommended name: Vitamin K-dependent protein C (EC:3.4.21.69)Alternative name(s): Anticoagulant protein C Autoprothrombin IIA Blood coagulation factor XIV Cleaved into the following 3 chains: |
Gene namesi | Name:Proc |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3411, Proc |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Golgi apparatus
- Golgi apparatus By similarity
Extracellular region or secreted
- Secreted By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Extracellular region or secreted
- extracellular space Source: RGD
Golgi apparatus
- Golgi apparatus Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
PropeptideiPRO_0000028127 | 19 – 41 | By similarityAdd BLAST | 23 | |
ChainiPRO_0000028128 | 42 – 461 | Vitamin K-dependent protein CAdd BLAST | 420 | |
ChainiPRO_0000028129 | 42 – 196 | Vitamin K-dependent protein C light chainBy similarityAdd BLAST | 155 | |
ChainiPRO_0000028130 | 199 – 461 | Vitamin K-dependent protein C heavy chainBy similarityAdd BLAST | 263 | |
PeptideiPRO_0000028131 | 199 – 212 | Activation peptideBy similarityAdd BLAST | 14 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 47 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 48 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 55 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 57 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Disulfide bondi | 58 ↔ 63 | By similarity | ||
Modified residuei | 60 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 61 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 66 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 67 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 70 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 76 | 4-carboxyglutamatePROSITE-ProRule annotationBy similarity | 1 | |
Disulfide bondi | 91 ↔ 110 | By similarity | ||
Disulfide bondi | 100 ↔ 105 | By similarity | ||
Disulfide bondi | 104 ↔ 119 | By similarity | ||
Modified residuei | 112 | (3R)-3-hydroxyaspartateBy similarity | 1 | |
Disulfide bondi | 121 ↔ 130 | By similarity | ||
Disulfide bondi | 139 ↔ 150 | By similarity | ||
Disulfide bondi | 146 ↔ 159 | By similarity | ||
Disulfide bondi | 161 ↔ 174 | By similarity | ||
Disulfide bondi | 182 ↔ 320 | Interchain (between light and heavy chains)PROSITE-ProRule annotation | ||
Glycosylationi | 215 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 239 ↔ 255 | By similarity | ||
Glycosylationi | 291 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 355 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 373 ↔ 387 | By similarity | ||
Disulfide bondi | 398 ↔ 426 | By similarity |
Post-translational modificationi
The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 212 – 213 | Cleavage; by thrombinBy similarity | 2 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Gamma-carboxyglutamic acid, Glycoprotein, Hydroxylation, ZymogenProteomic databases
PaxDbi | P31394 |
PRIDEi | P31394 |
PTM databases
GlyGeni | P31394, 3 sites |
PhosphoSitePlusi | P31394 |
Expressioni
Tissue specificityi
Plasma; synthesized in the liver.
Interactioni
Subunit structurei
Synthesized as a single chain precursor, which is cleaved into a light chain and a heavy chain held together by a disulfide bond. The enzyme is then activated by thrombin, which cleaves a tetradecapeptide from the amino end of the heavy chain; this reaction, which occurs at the surface of endothelial cells, is strongly promoted by thrombomodulin.
GO - Molecular functioni
- protein self-association Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000019596 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 42 – 87 | GlaPROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 96 – 131 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 135 – 175 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 41 | |
Domaini | 213 – 450 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 238 |
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QQ3W, Eukaryota |
InParanoidi | P31394 |
PhylomeDBi | P31394 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 4.10.740.10, 1 hit |
InterProi | View protein in InterPro IPR017857, Coagulation_fac-like_Gla_dom IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR035972, GLA-like_dom_SF IPR000294, GLA_domain IPR012224, Pept_S1A_FX IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00594, Gla, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001143, Factor_X, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN PR00001, GLABLOOD |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 1 hit SM00069, GLA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57630, SSF57630, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 2 hits PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00011, GLA_1, 1 hit PS50998, GLA_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P31394-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWQFRIFLLF ASTWGISGVS AHPDPVFSSS EGAHQVLRVR RANSFLEEVR
60 70 80 90 100
AGSLERECME EICDFEEAQE IFQNVEDTLA FWIKYFDGDQ CSTPPLDHQC
110 120 130 140 150
DSPCCGHGTC IDGLGGFSCS CDKGWEGRFC QQEMGFQDCR VKNGGCYHYC
160 170 180 190 200
LEETRGRRCR CAPGYELADD HMHCRPTVNF PCGKLWKRTD KKRKNFKRDI
210 220 230 240 250
DPEDEELELG PRIVNGTLTK QGDSPWQAIL LDSKKKLACG GVLIHTSWVL
260 270 280 290 300
TAAHCLESSK KLTVRLGEYD LRRRDPWELD LDIKEVLVHP NYTRSNSDND
310 320 330 340 350
IALLRLSQPA TLSKTIVPIC LPNSGLAQEL SQAGQETVVT GWGYQSDKVK
360 370 380 390 400
DGRRNRTFIL TFIRIPLAAR NDCMQVMNNV VSENMLCAGI IGDTRDACDG
410 420 430 440 450
DSGGPMVVFF RGTWFLVGLV SWGEGCGHLN NYGVYTKVGS YLKWIHSYIG
460
ERDVSLKSPK L
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF7FMY6 | F7FMY6_RAT | Protein C, isoform CRA_b | Proc rCG_49213 | 482 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64336 mRNA Translation: CAA45617.1 |
PIRi | S18994 |
RefSeqi | NP_036935.1, NM_012803.1 |
Genome annotation databases
GeneIDi | 25268 |
KEGGi | rno:25268 |
UCSCi | RGD:3411, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64336 mRNA Translation: CAA45617.1 |
PIRi | S18994 |
RefSeqi | NP_036935.1, NM_012803.1 |
3D structure databases
SMRi | P31394 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000019596 |
Protein family/group databases
MEROPSi | S01.218 |
PTM databases
GlyGeni | P31394, 3 sites |
PhosphoSitePlusi | P31394 |
Proteomic databases
PaxDbi | P31394 |
PRIDEi | P31394 |
Genome annotation databases
GeneIDi | 25268 |
KEGGi | rno:25268 |
UCSCi | RGD:3411, rat |
Organism-specific databases
CTDi | 5624 |
RGDi | 3411, Proc |
Phylogenomic databases
eggNOGi | ENOG502QQ3W, Eukaryota |
InParanoidi | P31394 |
PhylomeDBi | P31394 |
Enzyme and pathway databases
Reactomei | R-RNO-140837, Intrinsic Pathway of Fibrin Clot Formation R-RNO-140875, Common Pathway of Fibrin Clot Formation R-RNO-159740, Gamma-carboxylation of protein precursors R-RNO-159763, Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus R-RNO-159782, Removal of aminoterminal propeptides from gamma-carboxylated proteins R-RNO-202733, Cell surface interactions at the vascular wall R-RNO-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-RNO-8957275, Post-translational protein phosphorylation |
Miscellaneous databases
PROi | PR:P31394 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 4.10.740.10, 1 hit |
InterProi | View protein in InterPro IPR017857, Coagulation_fac-like_Gla_dom IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR035972, GLA-like_dom_SF IPR000294, GLA_domain IPR012224, Pept_S1A_FX IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00594, Gla, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001143, Factor_X, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN PR00001, GLABLOOD |
SMARTi | View protein in SMART SM00181, EGF, 2 hits SM00179, EGF_CA, 1 hit SM00069, GLA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57630, SSF57630, 1 hit |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 2 hits PS50026, EGF_3, 1 hit PS01187, EGF_CA, 1 hit PS00011, GLA_1, 1 hit PS50998, GLA_2, 1 hit PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PROC_RAT | |
Accessioni | P31394Primary (citable) accession number: P31394 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | April 7, 2021 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families