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Protein

Glucose-repressible alcohol dehydrogenase transcriptional effector

Gene

CCR4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as catalytic component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. CCR4 has 3'-5' RNase activity with a strong preference for polyadenylated substrates and also low exonuclease activity towards single-stranded DNA. Discovered because of its role in the control of ADH2 gene expression. It is required for the expression of genes involved in non-fermentative growth and it mediates or is required for the action of the SPT6 and SPT10 genes.4 Publications

Miscellaneous

Present with 2780 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi556Magnesium1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Exonuclease, Hydrolase, Nuclease, Repressor, RNA-binding
Biological processTranscription, Transcription regulation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28833-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-repressible alcohol dehydrogenase transcriptional effector (EC:3.1.13.4)
Alternative name(s):
Carbon catabolite repressor protein 4
Cytoplasmic deadenylase
Gene namesi
Name:CCR4
Ordered Locus Names:YAL021C
ORF Names:FUN27
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL021C
SGDiS000000019 CCR4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi556E → A: Loss of activity. 1 Publication1
Mutagenesisi713D → A: Strongly reduces activity. 1 Publication1
Mutagenesisi780D → A: Reduces activity. 1 Publication1
Mutagenesisi818H → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002185771 – 837Glucose-repressible alcohol dehydrogenase transcriptional effectorAdd BLAST837

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33PhosphothreonineCombined sources1
Modified residuei278PhosphoserineCombined sources1
Modified residuei285PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31384
PaxDbiP31384
PRIDEiP31384
TopDownProteomicsiP31384

PTM databases

CarbonylDBiP31384
iPTMnetiP31384

Interactioni

Subunit structurei

Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi31745, 3160 interactors
ComplexPortaliCPX-1800 Ccr4-Not complex
DIPiDIP-2522N
IntActiP31384, 55 interactors
MINTiP31384
STRINGi4932.YAL021C

Structurei

Secondary structure

1837
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP31384
SMRiP31384
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati334 – 356LRR 1Add BLAST23
Repeati358 – 379LRR 2Add BLAST22
Repeati381 – 402LRR 3Add BLAST22
Repeati404 – 426LRR 4Add BLAST23
Repeati427 – 447LRR 5Add BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi15 – 24Poly-Gln10
Compositional biasi77 – 103Asn-richAdd BLAST27
Compositional biasi89 – 103Poly-AsnAdd BLAST15
Compositional biasi190 – 206Gln-richAdd BLAST17

Domaini

The 169 C-terminal residues are important for deadenylase activity.

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074364
HOGENOMiHOG000294222
InParanoidiP31384
KOiK12603
OMAiFPNDKFP
OrthoDBiEOG092C0WZC

Family and domain databases

Gene3Di3.60.10.10, 1 hit
3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
PF13855 LRR_8, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 2 hits
SUPFAMiSSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 2 hits

Sequencei

Sequence statusi: Complete.

P31384-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNDPSLLGYP NVGPQQQQQQ QQQQHAGLLG KGTPNALQQQ LHMNQLTGIP
60 70 80 90 100
PPGLMNNSDV HTSSNNNSRQ LLDQLANGNA NMLNMNMDNN NNNNNNNNNN
110 120 130 140 150
NNNGGGSGVM MNASTAAVNS IGMVPTVGTP VNINVNASNP LLHPHLDDPS
160 170 180 190 200
LLNNPIWKLQ LHLAAVSAQS LGQPNIYARQ NAMKKYLATQ QAQQAQQQAQ
210 220 230 240 250
QQAQQQVPGP FGPGPQAAPP ALQPTDFQQS HIAEASKSLV DCTKQALMEM
260 270 280 290 300
ADTLTDSKTA KKQQPTGDST PSGTATNSAV STPLTPKIEL FANGKDEANQ
310 320 330 340 350
ALLQHKKLSQ YSIDEDDDIE NRMVMPKDSK YDDQLWHALD LSNLQIFNIS
360 370 380 390 400
ANIFKYDFLT RLYLNGNSLT ELPAEIKNLS NLRVLDLSHN RLTSLPAELG
410 420 430 440 450
SCFQLKYFYF FDNMVTTLPW EFGNLCNLQF LGVEGNPLEK QFLKILTEKS
460 470 480 490 500
VTGLIFYLRD NRPEIPLPHE RRFIEINTDG EPQREYDSLQ QSTEHLATDL
510 520 530 540 550
AKRTFTVLSY NTLCQHYATP KMYRYTPSWA LSWDYRRNKL KEQILSYDSD
560 570 580 590 600
LLCLQEVESK TFEEYWVPLL DKHGYTGIFH AKARAKTMHS KDSKKVDGCC
610 620 630 640 650
IFFKRDQFKL ITKDAMDFSG AWMKHKKFQR TEDYLNRAMN KDNVALFLKL
660 670 680 690 700
QHIPSGDTIW AVTTHLHWDP KFNDVKTFQV GVLLDHLETL LKEETSHNFR
710 720 730 740 750
QDIKKFPVLI CGDFNSYINS AVYELINTGR VQIHQEGNGR DFGYMSEKNF
760 770 780 790 800
SHNLALKSSY NCIGELPFTN FTPSFTDVID YIWFSTHALR VRGLLGEVDP
810 820 830
EYVSKFIGFP NDKFPSDHIP LLARFEFMKT NTGSKKV
Length:837
Mass (Da):94,670
Last modified:October 5, 2010 - v2
Checksum:i5CDB2E8FEDDBEF6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti544I → L in AAB24455 (PubMed:1459446).Curated1
Sequence conflicti803V → E in AAB24455 (PubMed:1459446).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S50459 Genomic DNA Translation: AAB24455.1
L05146 Genomic DNA Translation: AAC04936.1
BK006935 Genomic DNA Translation: DAA06967.1
PIRiS36713
RefSeqiNP_009381.1, NM_001178166.1

Genome annotation databases

EnsemblFungiiYAL021C; YAL021C; YAL021C
GeneIDi851212
KEGGisce:YAL021C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S50459 Genomic DNA Translation: AAB24455.1
L05146 Genomic DNA Translation: AAC04936.1
BK006935 Genomic DNA Translation: DAA06967.1
PIRiS36713
RefSeqiNP_009381.1, NM_001178166.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B8CX-ray3.41D/J/K/L111-837[»]
ProteinModelPortaliP31384
SMRiP31384
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31745, 3160 interactors
ComplexPortaliCPX-1800 Ccr4-Not complex
DIPiDIP-2522N
IntActiP31384, 55 interactors
MINTiP31384
STRINGi4932.YAL021C

PTM databases

CarbonylDBiP31384
iPTMnetiP31384

Proteomic databases

MaxQBiP31384
PaxDbiP31384
PRIDEiP31384
TopDownProteomicsiP31384

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL021C; YAL021C; YAL021C
GeneIDi851212
KEGGisce:YAL021C

Organism-specific databases

EuPathDBiFungiDB:YAL021C
SGDiS000000019 CCR4

Phylogenomic databases

GeneTreeiENSGT00550000074364
HOGENOMiHOG000294222
InParanoidiP31384
KOiK12603
OMAiFPNDKFP
OrthoDBiEOG092C0WZC

Enzyme and pathway databases

BioCyciYEAST:G3O-28833-MONOMER

Miscellaneous databases

PROiPR:P31384

Family and domain databases

Gene3Di3.60.10.10, 1 hit
3.80.10.10, 2 hits
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
PF13855 LRR_8, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 2 hits
SUPFAMiSSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS51450 LRR, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCCR4_YEAST
AccessioniPrimary (citable) accession number: P31384
Secondary accession number(s): D6VPJ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 5, 2010
Last modified: September 12, 2018
This is version 187 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names
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Main funding by: National Institutes of Health

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