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Protein

POU domain, class 3, transcription factor 2

Gene

Pou3f2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that plays a key role in neuronal differentiation (PubMed:24243019). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (PubMed:24243019).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi356 – 415HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 2
Short name:
Brain-2
Short name:
Brn-2
Nervous system-specific octamer-binding transcription factor N-Oct-3
Octamer-binding protein 7
Short name:
Oct-7
Octamer-binding transcription factor 7
Short name:
OTF-7
Gene namesi
Name:Pou3f2
Synonyms:Brn-2, Brn2, Otf7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:101895 Pou3f2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007231 – 445POU domain, class 3, transcription factor 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31360
PaxDbiP31360
PeptideAtlasiP31360
PRIDEiP31360

PTM databases

iPTMnetiP31360
PhosphoSitePlusiP31360

Expressioni

Tissue specificityi

Expressed specifically in the neuroectodermal cell lineage.

Gene expression databases

BgeeiENSMUSG00000095139 Expressed in 128 organ(s), highest expression level in thymus
CleanExiMM_POU3F2
GenevisibleiP31360 MM

Interactioni

Subunit structurei

Interacts with PQBP1 (By similarity). Interaction with ISL1 (PubMed:24643061).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202306, 1 interactor
STRINGi10090.ENSMUSP00000136147

Structurei

3D structure databases

ProteinModelPortaliP31360
SMRiP31360
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini264 – 338POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi68 – 90Poly-GlyAdd BLAST23
Compositional biasi125 – 149Poly-GlnAdd BLAST25

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
GeneTreeiENSGT00760000118935
HOGENOMiHOG000116303
HOVERGENiHBG053120
InParanoidiP31360
KOiK09365
OMAiSFTVNGM
OrthoDBiEOG091G0U0S
PhylomeDBiP31360
TreeFamiTF316413

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
IPR016362 TF_POU_3
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PIRSFiPIRSF002629 Transcription_factor_POU, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequencei

Sequence statusi: Complete.

P31360-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS
60 70 80 90 100
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ
110 120 130 140 150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQQ
160 170 180 190 200
QRPPHLVHHA ANHHPGPGAW RSAAAAAHLP PSMGASNGGL LYSQPSFTVN
210 220 230 240 250
GMLGAGGQPA GLHHHGLRDA HDEPHHADHH PHPHSHPHQQ PPPPPPPQGP
260 270 280 290 300
PGHPGAHHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL
310 320 330 340 350
YGNVFSQTTI CRFEALQLSF KNMCKLKPLL NKWLEEADSS SGSPTSIDKI
360 370 380 390 400
AAQGRKRKKR TSIEVSVKGA LESHFLKCPK PSAQEITSLA DSLQLEKEVV
410 420 430 440
RVWFCNRRQK EKRMTPPGGT LPGAEDVYGG SRDTPPHHGV QTPVQ
Length:445
Mass (Da):47,149
Last modified:July 1, 1993 - v1
Checksum:i1A47F10950EECE8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88300 Genomic DNA Translation: AAA39961.1
AL772230 Genomic DNA Translation: CAM22634.1
CCDSiCCDS18005.1
PIRiS31224
RefSeqiNP_032925.1, NM_008899.2
UniGeneiMm.129387
Mm.389520
Mm.487875

Genome annotation databases

EnsembliENSMUST00000178174; ENSMUSP00000136147; ENSMUSG00000095139
GeneIDi18992
KEGGimmu:18992
UCSCiuc008sdp.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88300 Genomic DNA Translation: AAA39961.1
AL772230 Genomic DNA Translation: CAM22634.1
CCDSiCCDS18005.1
PIRiS31224
RefSeqiNP_032925.1, NM_008899.2
UniGeneiMm.129387
Mm.389520
Mm.487875

3D structure databases

ProteinModelPortaliP31360
SMRiP31360
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202306, 1 interactor
STRINGi10090.ENSMUSP00000136147

PTM databases

iPTMnetiP31360
PhosphoSitePlusiP31360

Proteomic databases

MaxQBiP31360
PaxDbiP31360
PeptideAtlasiP31360
PRIDEiP31360

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000178174; ENSMUSP00000136147; ENSMUSG00000095139
GeneIDi18992
KEGGimmu:18992
UCSCiuc008sdp.2 mouse

Organism-specific databases

CTDi5454
MGIiMGI:101895 Pou3f2

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
GeneTreeiENSGT00760000118935
HOGENOMiHOG000116303
HOVERGENiHBG053120
InParanoidiP31360
KOiK09365
OMAiSFTVNGM
OrthoDBiEOG091G0U0S
PhylomeDBiP31360
TreeFamiTF316413

Miscellaneous databases

ChiTaRSiPou3f2 mouse
PROiPR:P31360
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000095139 Expressed in 128 organ(s), highest expression level in thymus
CleanExiMM_POU3F2
GenevisibleiP31360 MM

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
IPR016362 TF_POU_3
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PIRSFiPIRSF002629 Transcription_factor_POU, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPO3F2_MOUSE
AccessioniPrimary (citable) accession number: P31360
Secondary accession number(s): A2AJY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 12, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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