Pou3f2

Functionⁱ

Transcription factor that plays a key role in neuronal differentiation (PubMed:24243019). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (PubMed:24243019).By similarity3 Publications

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	356 – 415	HomeoboxPROSITE-ProRule annotationAdd BLAST		60

GO - Molecular functionⁱ

DNA binding
Source: MGI
Inferred from direct assayⁱ
- 12782656
- 15465489
DNA-binding transcription factor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 24243019
HMG box domain binding Source: MGI
identical protein binding Source: MGI
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 15465489
sequence-specific DNA binding
Source: MGI
Inferred from direct assayⁱ
- 17141158
transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 15465489
transcription coactivator activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18505825

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

astrocyte development
Source: MGI
Inferred from mutant phenotypeⁱ
- 8543156
cellular response to organic substance
Source: MGI
Inferred from direct assayⁱ
- 18794345
cerebral cortex radially oriented cell migration
Source: MGI
Inferred from genetic interactionⁱ
- 12130536
epidermis development
Source: MGI
Inferred from mutant phenotypeⁱ
- 8543156
forebrain ventricular zone progenitor cell division
Source: MGI
Inferred from genetic interactionⁱ
- 12130536
hypothalamus cell differentiation
Source: MGI
Inferred from mutant phenotypeⁱ
- 8543156
myelination in peripheral nervous system
Source: MGI
Inferred from genetic interactionⁱ
- 12782656
negative regulation of gene expression
Source: CACAO
Inferred from mutant phenotypeⁱ
- 23069940
nervous system development
Source: UniProtKB
Inferred from direct assayⁱ
- 24243019
neurohypophysis development
Source: MGI
Inferred from mutant phenotypeⁱ
- 8543156
neuron development
Source: UniProtKB
Inferred from direct assayⁱ
- 24243019
neuron differentiation
Source: UniProtKB
Inferred from direct assayⁱ
- 24243019
neuron fate commitment
Source: UniProtKB
Inferred from direct assayⁱ
- 24243019
neuron fate specification
Source: UniProtKB
Inferred from direct assayⁱ
- 24243019
positive regulation of cell proliferation
Source: MGI
Inferred from sequence orthologyⁱ
- 15024079
positive regulation of multicellular organism growth
Source: MGI
Inferred from mutant phenotypeⁱ
- 8543156
positive regulation of transcription by RNA polymerase II
Source: NTNU_SB
Inferred from direct assayⁱ
- 15465489
regulation of axonogenesis
Source: MGI
Inferred from mutant phenotypeⁱ
- 8543156
regulation of cell differentiation
Source: MGI
Inferred from mutant phenotypeⁱ
- 8593698
regulation of transcription, DNA-templated Source: MGI
regulation of transcription by RNA polymerase II
Source: UniProtKB
Inferred from direct assayⁱ
- 24243019
Schwann cell development
Source: MGI
Inferred from genetic interactionⁱ
- 12782656

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Differentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: POU domain, class 3, transcription factor 2 Alternative name(s): Brain-specific homeobox/POU domain protein 2 Short name: Brain-2 Short name: Brn-2 Nervous system-specific octamer-binding transcription factor N-Oct-3 Octamer-binding protein 7 Short name: Oct-7 Octamer-binding transcription factor 7 Short name: OTF-7
Gene namesⁱ	Name:Pou3f2 Synonyms:Brn-2, Brn2, Otf7
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 4

Organism-specific databases

MGIⁱ	MGI:101895 Pou3f2

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000100723	1 – 445	POU domain, class 3, transcription factor 2Add BLAST		445

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	343	PhosphoserineBy similarity		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P31360
PaxDbⁱ	P31360
PeptideAtlas More...PeptideAtlasⁱ	P31360
PRIDEⁱ	P31360

PTM databases

iPTMnetⁱ	P31360
PhosphoSitePlusⁱ	P31360

Expressionⁱ

Tissue specificityⁱ

Expressed specifically in the neuroectodermal cell lineage.

Gene expression databases

Bgeeⁱ	ENSMUSG00000095139 Expressed in 128 organ(s), highest expression level in thymus
CleanExⁱ	MM_POU3F2
Genevisibleⁱ	P31360 MM

Interactionⁱ

Subunit structureⁱ

Interacts with PQBP1 (By similarity). Interaction with ISL1 (PubMed:24643061).By similarity1 Publication

GO - Molecular functionⁱ

HMG box domain binding Source: MGI
identical protein binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	202306, 1 interactor
STRINGⁱ	10090.ENSMUSP00000136147

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P31360
SMRⁱ	P31360
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	264 – 338	POU-specificPROSITE-ProRule annotationAdd BLAST		75

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	68 – 90	Poly-GlyAdd BLAST		23
Compositional biasⁱ	125 – 149	Poly-GlnAdd BLAST		25

Sequence similaritiesⁱ

Belongs to the POU transcription factor family. Class-3 subfamily.Curated

Keywords - Domainⁱ

Homeobox

Phylogenomic databases

eggNOGⁱ	KOG3802 Eukaryota ENOG410XQ7X LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118935
HOGENOMⁱ	HOG000116303
HOVERGENⁱ	HBG053120
InParanoidⁱ	P31360
KEGG Orthology (KO) More...KOⁱ	K09365
OMAⁱ	SFTVNGM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0U0S
PhylomeDBⁱ	P31360
TreeFamⁱ	TF316413

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00086 homeodomain, 1 hit
InterProⁱ	View protein in InterPro IPR009057 Homeobox-like_sf IPR017970 Homeobox_CS IPR001356 Homeobox_dom IPR010982 Lambda_DNA-bd_dom_sf IPR013847 POU IPR000327 POU_dom IPR016362 TF_POU_3
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00046 Homeobox, 1 hit PF00157 Pou, 1 hit
PIRSFⁱ	PIRSF002629 Transcription_factor_POU, 1 hit
PRINTSⁱ	PR00028 POUDOMAIN
SMARTⁱ	View protein in SMART SM00389 HOX, 1 hit SM00352 POU, 1 hit
SUPFAMⁱ	SSF46689 SSF46689, 1 hit SSF47413 SSF47413, 1 hit
PROSITEⁱ	View protein in PROSITE PS00027 HOMEOBOX_1, 1 hit PS50071 HOMEOBOX_2, 1 hit PS00035 POU_1, 1 hit PS00465 POU_2, 1 hit PS51179 POU_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P31360-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS 
        60         70         80         90        100
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ 
       110        120        130        140        150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQQ 
       160        170        180        190        200
QRPPHLVHHA ANHHPGPGAW RSAAAAAHLP PSMGASNGGL LYSQPSFTVN 
       210        220        230        240        250
GMLGAGGQPA GLHHHGLRDA HDEPHHADHH PHPHSHPHQQ PPPPPPPQGP 
       260        270        280        290        300
PGHPGAHHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL 
       310        320        330        340        350
YGNVFSQTTI CRFEALQLSF KNMCKLKPLL NKWLEEADSS SGSPTSIDKI 
       360        370        380        390        400
AAQGRKRKKR TSIEVSVKGA LESHFLKCPK PSAQEITSLA DSLQLEKEVV 
       410        420        430        440 
RVWFCNRRQK EKRMTPPGGT LPGAEDVYGG SRDTPPHHGV QTPVQ

Length:445

Mass (Da):47,149

Last modified:July 1, 1993 - v1

Checksum:ⁱ1A47F10950EECE8A

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M88300 Genomic DNA Translation: AAA39961.1 AL772230 Genomic DNA Translation: CAM22634.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS18005.1
PIRⁱ	S31224
NCBI Reference Sequences More...RefSeqⁱ	NP_032925.1, NM_008899.2
UniGeneⁱ	Mm.129387 Mm.389520 Mm.487875

Genome annotation databases

Ensemblⁱ	ENSMUST00000178174; ENSMUSP00000136147; ENSMUSG00000095139
GeneIDⁱ	18992
KEGGⁱ	mmu:18992
UCSC genome browser More...UCSCⁱ	uc008sdp.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P31360	POU domain, class 3, transcription factor 2 (Fragment)		MACMU		77	UniRef100_P31360

Protein	Similar proteins		Species	Score	Length	Source
P31360	POU domain, class 3, transcription factor 2		RAT		445	UniRef90_P31360
POU domain, class 3, transcription factor 2		HUMAN		443
POU domain, class 3, transcription factor 2 (Fragment)		MACMU		77
POU domain protein		MESAU		445
POU domain protein		MONDO		444
+33

Protein	Similar proteins		Species	Score	Length	Source
P31360	POU domain, class 3, transcription factor 2		RAT		445	UniRef50_P31360
POU domain, class 3, transcription factor 2		HUMAN		443
POU domain, class 3, transcription factor 2-B		XENLA		385
POU domain, class 3, transcription factor 4		MESAU		361
POU domain, class 3, transcription factor 4		MOUSE		361
+206

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M88300 Genomic DNA Translation: AAA39961.1 AL772230 Genomic DNA Translation: CAM22634.1
CCDSⁱ	CCDS18005.1
PIRⁱ	S31224
RefSeqⁱ	NP_032925.1, NM_008899.2
UniGeneⁱ	Mm.129387 Mm.389520 Mm.487875

3D structure databases

ProteinModelPortalⁱ	P31360
SMRⁱ	P31360
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	202306, 1 interactor
STRINGⁱ	10090.ENSMUSP00000136147

PTM databases

iPTMnetⁱ	P31360
PhosphoSitePlusⁱ	P31360

Proteomic databases

MaxQBⁱ	P31360
PaxDbⁱ	P31360
PeptideAtlasⁱ	P31360
PRIDEⁱ	P31360

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000178174; ENSMUSP00000136147; ENSMUSG00000095139
GeneIDⁱ	18992
KEGGⁱ	mmu:18992
UCSCⁱ	uc008sdp.2 mouse

Organism-specific databases

CTDⁱ	5454
MGIⁱ	MGI:101895 Pou3f2

Phylogenomic databases

eggNOGⁱ	KOG3802 Eukaryota ENOG410XQ7X LUCA
GeneTreeⁱ	ENSGT00760000118935
HOGENOMⁱ	HOG000116303
HOVERGENⁱ	HBG053120
InParanoidⁱ	P31360
KOⁱ	K09365
OMAⁱ	SFTVNGM
OrthoDBⁱ	EOG091G0U0S
PhylomeDBⁱ	P31360
TreeFamⁱ	TF316413

Miscellaneous databases

ChiTaRSⁱ	Pou3f2 mouse
Protein Ontology More...PROⁱ	PR:P31360
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000095139 Expressed in 128 organ(s), highest expression level in thymus
CleanExⁱ	MM_POU3F2
Genevisibleⁱ	P31360 MM

Family and domain databases

CDDⁱ	cd00086 homeodomain, 1 hit
InterProⁱ	View protein in InterPro IPR009057 Homeobox-like_sf IPR017970 Homeobox_CS IPR001356 Homeobox_dom IPR010982 Lambda_DNA-bd_dom_sf IPR013847 POU IPR000327 POU_dom IPR016362 TF_POU_3
Pfamⁱ	View protein in Pfam PF00046 Homeobox, 1 hit PF00157 Pou, 1 hit
PIRSFⁱ	PIRSF002629 Transcription_factor_POU, 1 hit
PRINTSⁱ	PR00028 POUDOMAIN
SMARTⁱ	View protein in SMART SM00389 HOX, 1 hit SM00352 POU, 1 hit
SUPFAMⁱ	SSF46689 SSF46689, 1 hit SSF47413 SSF47413, 1 hit
PROSITEⁱ	View protein in PROSITE PS00027 HOMEOBOX_1, 1 hit PS50071 HOMEOBOX_2, 1 hit PS00035 POU_1, 1 hit PS00465 POU_2, 1 hit PS51179 POU_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PO3F2_MOUSE
Accessionⁱ	P31360Primary (citable) accession number: P31360 Secondary accession number(s): A2AJY1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
	Last sequence update:	July 1, 1993
	Last modified:	September 12, 2018
	This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P31360 (PO3F2_MOUSE)

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POU domain, class 3, transcription factor 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ