UniProtKB - P31360 (PO3F2_MOUSE)
POU domain, class 3, transcription factor 2
Pou3f2
Functioni
Transcription factor that plays a key role in neuronal differentiation (PubMed:24243019).
Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity).
Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (PubMed:18505825, PubMed:18403418).
The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220).
Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (PubMed:24243019).
By similarity5 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 356 – 415 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- HMG box domain binding Source: MGI
- identical protein binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- astrocyte development Source: MGI
- cellular response to organic substance Source: MGI
- cerebral cortex radially oriented cell migration Source: MGI
- epidermis development Source: MGI
- forebrain ventricular zone progenitor cell division Source: MGI
- hypothalamus cell differentiation Source: MGI
- myelination in peripheral nervous system Source: MGI
- negative regulation of gene expression Source: CACAO
- nervous system development Source: UniProtKB
- neurohypophysis development Source: MGI
- neuron development Source: UniProtKB
- neuron differentiation Source: UniProtKB
- neuron fate commitment Source: UniProtKB
- neuron fate specification Source: UniProtKB
- positive regulation of cell population proliferation Source: MGI
- positive regulation of multicellular organism growth Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of axonogenesis Source: MGI
- regulation of cell differentiation Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: UniProtKB
- Schwann cell development Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: POU domain, class 3, transcription factor 2Alternative name(s): Brain-specific homeobox/POU domain protein 2 Short name: Brain-2 Short name: Brn-2 Nervous system-specific octamer-binding transcription factor N-Oct-3 Octamer-binding protein 7 Short name: Oct-7 Octamer-binding transcription factor 7 Short name: OTF-7 |
Gene namesi | Name:Pou3f2 Synonyms:Brn-2, Brn2, Otf7 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101895, Pou3f2 |
VEuPathDBi | HostDB:ENSMUSG00000095139 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100723 | 1 – 445 | POU domain, class 3, transcription factor 2Add BLAST | 445 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 343 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P31360 |
PaxDbi | P31360 |
PeptideAtlasi | P31360 |
PRIDEi | P31360 |
ProteomicsDBi | 289649 |
PTM databases
iPTMneti | P31360 |
PhosphoSitePlusi | P31360 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000095139, Expressed in thymus and 150 other tissues |
Genevisiblei | P31360, MM |
Interactioni
Subunit structurei
Interacts with PQBP1 (By similarity). Interaction with ISL1 (PubMed:24643061).
By similarity1 PublicationGO - Molecular functioni
- HMG box domain binding Source: MGI
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202306, 1 interactor |
STRINGi | 10090.ENSMUSP00000136147 |
Miscellaneous databases
RNActi | P31360, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 264 – 338 | POU-specificPROSITE-ProRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 64 – 173 | DisorderedSequence analysisAdd BLAST | 110 | |
Regioni | 203 – 269 | DisorderedSequence analysisAdd BLAST | 67 | |
Regioni | 411 – 445 | DisorderedSequence analysisAdd BLAST | 35 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 123 – 155 | Polar residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 214 – 230 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 237 – 254 | Pro residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000162208 |
HOGENOMi | CLU_013065_1_2_1 |
InParanoidi | P31360 |
OMAi | HHEHSDE |
OrthoDBi | 752252at2759 |
PhylomeDBi | P31360 |
TreeFami | TF316413 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR016362, TF_POU_3 |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PIRSFi | PIRSF002629, Transcription_factor_POU, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS
60 70 80 90 100
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ
110 120 130 140 150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQQ
160 170 180 190 200
QRPPHLVHHA ANHHPGPGAW RSAAAAAHLP PSMGASNGGL LYSQPSFTVN
210 220 230 240 250
GMLGAGGQPA GLHHHGLRDA HDEPHHADHH PHPHSHPHQQ PPPPPPPQGP
260 270 280 290 300
PGHPGAHHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL
310 320 330 340 350
YGNVFSQTTI CRFEALQLSF KNMCKLKPLL NKWLEEADSS SGSPTSIDKI
360 370 380 390 400
AAQGRKRKKR TSIEVSVKGA LESHFLKCPK PSAQEITSLA DSLQLEKEVV
410 420 430 440
RVWFCNRRQK EKRMTPPGGT LPGAEDVYGG SRDTPPHHGV QTPVQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88300 Genomic DNA Translation: AAA39961.1 AL772230 Genomic DNA No translation available. |
CCDSi | CCDS18005.1 |
PIRi | S31224 |
RefSeqi | NP_032925.1, NM_008899.2 |
Genome annotation databases
Ensembli | ENSMUST00000178174; ENSMUSP00000136147; ENSMUSG00000095139 |
GeneIDi | 18992 |
KEGGi | mmu:18992 |
UCSCi | uc008sdp.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M88300 Genomic DNA Translation: AAA39961.1 AL772230 Genomic DNA No translation available. |
CCDSi | CCDS18005.1 |
PIRi | S31224 |
RefSeqi | NP_032925.1, NM_008899.2 |
3D structure databases
SMRi | P31360 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202306, 1 interactor |
STRINGi | 10090.ENSMUSP00000136147 |
PTM databases
iPTMneti | P31360 |
PhosphoSitePlusi | P31360 |
Proteomic databases
MaxQBi | P31360 |
PaxDbi | P31360 |
PeptideAtlasi | P31360 |
PRIDEi | P31360 |
ProteomicsDBi | 289649 |
Protocols and materials databases
Antibodypediai | 18892, 363 antibodies from 42 providers |
DNASUi | 18992 |
Genome annotation databases
Ensembli | ENSMUST00000178174; ENSMUSP00000136147; ENSMUSG00000095139 |
GeneIDi | 18992 |
KEGGi | mmu:18992 |
UCSCi | uc008sdp.2, mouse |
Organism-specific databases
CTDi | 5454 |
MGIi | MGI:101895, Pou3f2 |
VEuPathDBi | HostDB:ENSMUSG00000095139 |
Phylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000162208 |
HOGENOMi | CLU_013065_1_2_1 |
InParanoidi | P31360 |
OMAi | HHEHSDE |
OrthoDBi | 752252at2759 |
PhylomeDBi | P31360 |
TreeFami | TF316413 |
Miscellaneous databases
BioGRID-ORCSi | 18992, 0 hits in 64 CRISPR screens |
ChiTaRSi | Pou3f2, mouse |
PROi | PR:P31360 |
RNActi | P31360, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000095139, Expressed in thymus and 150 other tissues |
Genevisiblei | P31360, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR016362, TF_POU_3 |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PIRSFi | PIRSF002629, Transcription_factor_POU, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PO3F2_MOUSE | |
Accessioni | P31360Primary (citable) accession number: P31360 Secondary accession number(s): A2AJY1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families