POU domain, class 3, transcription factor 2

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• DNA binding Source: UniProtKB <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • Ref.4

    "The three SoxC proteins--Sox4, Sox11 and Sox12--exhibit overlapping expression patterns and molecular properties."
    Dy P., Penzo-Mendez A., Wang H., Pedraza C.E., Macklin W.B., Lefebvre V.
    Nucleic Acids Res. 36:3101-3117(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SBInferred from direct assayⁱ
  • "Hox/Pbx and Brn binding sites mediate Pax3 expression in vitro and in vivo."
    Pruitt S.C., Bussman A., Maslov A.Y., Natoli T.A., Heinaman R.
    Gene Expr Patterns 4:671-685(2004) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons."
    Wapinski O.L., Vierbuchen T., Qu K., Lee Q.Y., Chanda S., Fuentes D.R., Giresi P.G., Ng Y.H., Marro S., Neff N.F., Drechsel D., Martynoga B., Castro D.S., Webb A.E., Suedhof T.C., Brunet A., Guillemot F., Chang H.Y., Wernig M.
    Cell 155:621-635(2013) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• HMG box domain binding Source: MGI
• identical protein binding Source: MGI
• RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SBInferred from direct assayⁱ
  • "Hox/Pbx and Brn binding sites mediate Pax3 expression in vitro and in vivo."
    Pruitt S.C., Bussman A., Maslov A.Y., Natoli T.A., Heinaman R.
    Gene Expr Patterns 4:671-685(2004) [PubMed] [Europe PMC] [Abstract]
• sequence-specific DNA binding Source: MGIInferred from direct assayⁱ
  • "Proneural bHLH and Brn proteins coregulate a neurogenic program through cooperative binding to a conserved DNA motif."
    Castro D.S., Skowronska-Krawczyk D., Armant O., Donaldson I.J., Parras C., Hunt C., Critchley J.A., Nguyen L., Gossler A., Gottgens B., Matter J.M., Guillemot F.
    Dev Cell 11:831-844(2006) [PubMed] [Europe PMC] [Abstract]
• sequence-specific double-stranded DNA binding Source: MGI

GO - Biological processⁱ

• astrocyte development Source: MGI <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2."
    Schonemann M.D., Ryan A.K., McEvilly R.J., O'Connell S.M., Arias C.A., Kalla K.A., Li P., Sawchenko P.E., Rosenfeld M.G.
    Genes Dev 9:3122-3135(1995) [PubMed] [Europe PMC] [Abstract]
• cellular response to organic substance Source: MGIInferred from direct assayⁱ
  • "The T-box transcription factor Eomes/Tbr2 regulates neurogenesis in the cortical subventricular zone."
    Arnold S.J., Huang G.J., Cheung A.F., Era T., Nishikawa S., Bikoff E.K., Molnar Z., Robertson E.J., Groszer M.
    Genes Dev 22:2479-2484(2008) [PubMed] [Europe PMC] [Abstract]
• cerebral cortex radially oriented cell migration Source: MGI <p>Inferred from Genetic Interaction</p> <p>Used to describe "traditional" genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • "Brn-1 and Brn-2 share crucial roles in the production and positioning of mouse neocortical neurons."
    Sugitani Y., Nakai S., Minowa O., Nishi M., Jishage K., Kawano H., Mori K., Ogawa M., Noda T.
    Genes Dev. 16:1760-1765(2002) [PubMed] [Europe PMC] [Abstract]
• epidermis development Source: MGIInferred from mutant phenotypeⁱ
  • "Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2."
    Schonemann M.D., Ryan A.K., McEvilly R.J., O'Connell S.M., Arias C.A., Kalla K.A., Li P., Sawchenko P.E., Rosenfeld M.G.
    Genes Dev 9:3122-3135(1995) [PubMed] [Europe PMC] [Abstract]
• forebrain ventricular zone progenitor cell division Source: MGIInferred from genetic interactionⁱ
  • "Brn-1 and Brn-2 share crucial roles in the production and positioning of mouse neocortical neurons."
    Sugitani Y., Nakai S., Minowa O., Nishi M., Jishage K., Kawano H., Mori K., Ogawa M., Noda T.
    Genes Dev. 16:1760-1765(2002) [PubMed] [Europe PMC] [Abstract]
• hypothalamus cell differentiation Source: MGIInferred from mutant phenotypeⁱ
  • "Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2."
    Schonemann M.D., Ryan A.K., McEvilly R.J., O'Connell S.M., Arias C.A., Kalla K.A., Li P., Sawchenko P.E., Rosenfeld M.G.
    Genes Dev 9:3122-3135(1995) [PubMed] [Europe PMC] [Abstract]
• myelination in peripheral nervous system Source: MGIInferred from genetic interactionⁱ
  • "The POU proteins Brn-2 and Oct-6 share important functions in Schwann cell development."
    Jaegle M., Ghazvini M., Mandemakers W., Piirsoo M., Driegen S., Levavasseur F., Raghoenath S., Grosveld F., Meijer D.
    Genes Dev 17:1380-1391(2003) [PubMed] [Europe PMC] [Abstract]
• negative regulation of gene expression Source: CACAOInferred from mutant phenotypeⁱ
  • "BRN2 is a transcriptional repressor of CDH13 (T-cadherin) in melanoma cells."
    Ellmann L., Joshi M.B., Resink T.J., Bosserhoff A.K., Kuphal S.
    Lab Invest 92:1788-1800(2012) [PubMed] [Europe PMC] [Abstract]
• nervous system development Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons."
    Wapinski O.L., Vierbuchen T., Qu K., Lee Q.Y., Chanda S., Fuentes D.R., Giresi P.G., Ng Y.H., Marro S., Neff N.F., Drechsel D., Martynoga B., Castro D.S., Webb A.E., Suedhof T.C., Brunet A., Guillemot F., Chang H.Y., Wernig M.
    Cell 155:621-635(2013) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• neurohypophysis development Source: MGIInferred from mutant phenotypeⁱ
  • "Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2."
    Schonemann M.D., Ryan A.K., McEvilly R.J., O'Connell S.M., Arias C.A., Kalla K.A., Li P., Sawchenko P.E., Rosenfeld M.G.
    Genes Dev 9:3122-3135(1995) [PubMed] [Europe PMC] [Abstract]
• neuron development Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons."
    Wapinski O.L., Vierbuchen T., Qu K., Lee Q.Y., Chanda S., Fuentes D.R., Giresi P.G., Ng Y.H., Marro S., Neff N.F., Drechsel D., Martynoga B., Castro D.S., Webb A.E., Suedhof T.C., Brunet A., Guillemot F., Chang H.Y., Wernig M.
    Cell 155:621-635(2013) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• neuron differentiation Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons."
    Wapinski O.L., Vierbuchen T., Qu K., Lee Q.Y., Chanda S., Fuentes D.R., Giresi P.G., Ng Y.H., Marro S., Neff N.F., Drechsel D., Martynoga B., Castro D.S., Webb A.E., Suedhof T.C., Brunet A., Guillemot F., Chang H.Y., Wernig M.
    Cell 155:621-635(2013) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• neuron fate commitment Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons."
    Wapinski O.L., Vierbuchen T., Qu K., Lee Q.Y., Chanda S., Fuentes D.R., Giresi P.G., Ng Y.H., Marro S., Neff N.F., Drechsel D., Martynoga B., Castro D.S., Webb A.E., Suedhof T.C., Brunet A., Guillemot F., Chang H.Y., Wernig M.
    Cell 155:621-635(2013) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• neuron fate specification Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons."
    Wapinski O.L., Vierbuchen T., Qu K., Lee Q.Y., Chanda S., Fuentes D.R., Giresi P.G., Ng Y.H., Marro S., Neff N.F., Drechsel D., Martynoga B., Castro D.S., Webb A.E., Suedhof T.C., Brunet A., Guillemot F., Chang H.Y., Wernig M.
    Cell 155:621-635(2013) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• positive regulation of cell population proliferation Source: MGIInferred from sequence orthologyⁱ
  • "Brn-2 expression controls melanoma proliferation and is directly regulated by beta-catenin."
    Goodall J., Martinozzi S., Dexter T.J., Champeval D., Carreira S., Larue L., Goding C.R.
    Mol Cell Biol 24:2915-2922(2004) [PubMed] [Europe PMC] [Abstract]
• positive regulation of multicellular organism growth Source: MGIInferred from mutant phenotypeⁱ
  • "Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2."
    Schonemann M.D., Ryan A.K., McEvilly R.J., O'Connell S.M., Arias C.A., Kalla K.A., Li P., Sawchenko P.E., Rosenfeld M.G.
    Genes Dev 9:3122-3135(1995) [PubMed] [Europe PMC] [Abstract]
• positive regulation of transcription by RNA polymerase II Source: UniProtKBInferred from direct assayⁱ
  • Ref.4

    "The three SoxC proteins--Sox4, Sox11 and Sox12--exhibit overlapping expression patterns and molecular properties."
    Dy P., Penzo-Mendez A., Wang H., Pedraza C.E., Macklin W.B., Lefebvre V.
    Nucleic Acids Res. 36:3101-3117(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• regulation of axonogenesis Source: MGIInferred from mutant phenotypeⁱ
  • "Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2."
    Schonemann M.D., Ryan A.K., McEvilly R.J., O'Connell S.M., Arias C.A., Kalla K.A., Li P., Sawchenko P.E., Rosenfeld M.G.
    Genes Dev 9:3122-3135(1995) [PubMed] [Europe PMC] [Abstract]
• regulation of cell differentiation Source: MGIInferred from mutant phenotypeⁱ
  • "Preferential differentiation of P19 mouse embryonal carcinoma cells into smooth muscle cells. Use of retinoic acid and antisense against the central nervous system-specific POU transcription factor Brn-2."
    Suzuki T., Kim H.S., Kurabayashi M., Hamada H., Fujii H., Aikawa M., Watanabe M., Watanabe N., Sakomura Y., Yazaki Y., Nagai R.
    Circ. Res. 78:395-404(1996) [PubMed] [Europe PMC] [Abstract]
• regulation of transcription, DNA-templated Source: MGI
• regulation of transcription by RNA polymerase II Source: UniProtKBInferred from direct assayⁱ
  • Ref.6

    "Hierarchical mechanisms for direct reprogramming of fibroblasts to neurons."
    Wapinski O.L., Vierbuchen T., Qu K., Lee Q.Y., Chanda S., Fuentes D.R., Giresi P.G., Ng Y.H., Marro S., Neff N.F., Drechsel D., Martynoga B., Castro D.S., Webb A.E., Suedhof T.C., Brunet A., Guillemot F., Chang H.Y., Wernig M.
    Cell 155:621-635(2013) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION.
• Schwann cell development Source: MGIInferred from genetic interactionⁱ
  • "The POU proteins Brn-2 and Oct-6 share important functions in Schwann cell development."
    Jaegle M., Ghazvini M., Mandemakers W., Piirsoo M., Driegen S., Levavasseur F., Raghoenath S., Grosveld F., Meijer D.
    Genes Dev 17:1380-1391(2003) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

• UniProt annotation
• GO - Cellular component

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityⁱ

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

GO - Molecular functionⁱ

• HMG box domain binding Source: MGI
• identical protein binding Source: MGI

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

Region

Compositional bias

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS 
        60         70         80         90        100
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ 
       110        120        130        140        150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQQ 
       160        170        180        190        200
QRPPHLVHHA ANHHPGPGAW RSAAAAAHLP PSMGASNGGL LYSQPSFTVN 
       210        220        230        240        250
GMLGAGGQPA GLHHHGLRDA HDEPHHADHH PHPHSHPHQQ PPPPPPPQGP 
       260        270        280        290        300
PGHPGAHHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL 
       310        320        330        340        350
YGNVFSQTTI CRFEALQLSF KNMCKLKPLL NKWLEEADSS SGSPTSIDKI 
       360        370        380        390        400
AAQGRKRKKR TSIEVSVKGA LESHFLKCPK PSAQEITSLA DSLQLEKEVV 
       410        420        430        440 
RVWFCNRRQK EKRMTPPGGT LPGAEDVYGG SRDTPPHHGV QTPVQ      

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. MGD cross-references
   Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
2. SIMILARITY comments
   Index of protein domains and families