UniProtKB - P31324 (KAP3_MOUSE)
cAMP-dependent protein kinase type II-beta regulatory subunit
Prkar2b
Functioni
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 221 | 3',5'-cAMP 1By similarity | 1 | |
Binding sitei | 230 | 3',5'-cAMP 1By similarity | 1 | |
Binding sitei | 350 | 3',5'-cAMP 2By similarity | 1 | |
Binding sitei | 359 | 3',5'-cAMP 2By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 152 – 273 | 3',5'-cAMP 1Add BLAST | 122 | |
Nucleotide bindingi | 274 – 416 | 3',5'-cAMP 2Add BLAST | 143 |
GO - Molecular functioni
- cAMP binding Source: MGI
- cAMP-dependent protein kinase inhibitor activity Source: MGI
- cAMP-dependent protein kinase regulator activity Source: MGI
- protein domain specific binding Source: MGI
- protein kinase A catalytic subunit binding Source: MGI
- protein kinase binding Source: ParkinsonsUK-UCL
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- fatty acid metabolic process Source: MGI
- learning Source: MGI
- modulation of chemical synaptic transmission Source: SynGO
- negative regulation of cAMP-dependent protein kinase activity Source: MGI
- regulation of protein kinase activity Source: MGI
- response to antipsychotic drug Source: Ensembl
- response to clozapine Source: Ensembl
Keywordsi
Ligand | cAMP, cAMP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-163615, PKA activation R-MMU-164378, PKA activation in glucagon signalling R-MMU-180024, DARPP-32 events R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-MMU-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-MMU-5610787, Hedgehog 'off' state R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-8854518, AURKA Activation by TPX2 R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: cAMP-dependent protein kinase type II-beta regulatory subunit |
Gene namesi | Name:Prkar2b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97760, Prkar2b |
VEuPathDBi | HostDB:ENSMUSG00000002997 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: Colocalizes with PJA2 in the cytoplasm and at the cell membrane.By similarity
Cytoskeleton
- centrosome Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cAMP-dependent protein kinase complex Source: MGI
- ciliary base Source: MGI
- cytoplasm Source: MGI
- dendrite Source: MGI
- dendritic shaft Source: ParkinsonsUK-UCL
- dendritic spine Source: ParkinsonsUK-UCL
- glutamatergic synapse Source: SynGO
- membrane raft Source: MGI
- neuronal cell body Source: MGI
- perinuclear region of cytoplasm Source: MGI
- postsynapse Source: SynGO
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205391 | 1 – 416 | cAMP-dependent protein kinase type II-beta regulatory subunitAdd BLAST | 416 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 83 | PhosphoserineCombined sources | 1 | |
Modified residuei | 85 | PhosphoserineBy similarity | 1 | |
Modified residuei | 112 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P31324 |
jPOSTi | P31324 |
MaxQBi | P31324 |
PaxDbi | P31324 |
PeptideAtlasi | P31324 |
PRIDEi | P31324 |
ProteomicsDBi | 301730 |
PTM databases
iPTMneti | P31324 |
PhosphoSitePlusi | P31324 |
SwissPalmi | P31324 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000002997, Expressed in female gonad and 271 other tissues |
ExpressionAtlasi | P31324, baseline and differential |
Genevisiblei | P31324, MM |
Interactioni
Subunit structurei
The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.
Interacts with PRKACA and PRKACB.
Interacts with the phosphorylated form of PJA2.
Forms a complex composed of PRKAR2B, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity.
By similarityBinary interactionsi
GO - Molecular functioni
- protein domain specific binding Source: MGI
- protein kinase A catalytic subunit binding Source: MGI
- protein kinase binding Source: ParkinsonsUK-UCL
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202369, 29 interactors |
CORUMi | P31324 |
DIPi | DIP-31571N |
IntActi | P31324, 18 interactors |
MINTi | P31324 |
STRINGi | 10090.ENSMUSP00000039797 |
Miscellaneous databases
RNActi | P31324, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P31324 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 151 | Dimerization and phosphorylationAdd BLAST | 151 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1113, Eukaryota |
GeneTreei | ENSGT00940000158160 |
HOGENOMi | CLU_018310_2_0_1 |
InParanoidi | P31324 |
OMAi | QYNFGRR |
OrthoDBi | 1047290at2759 |
PhylomeDBi | P31324 |
TreeFami | TF314920 |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits |
DisProti | DP01944 |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR012198, cAMP_dep_PK_reg_su IPR003117, cAMP_dep_PK_reg_su_I/II_a/b IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF02197, RIIa, 1 hit |
PIRSFi | PIRSF000548, PK_regulatory, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00394, RIIa, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits |
PROSITEi | View protein in PROSITE PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSIEIPAGLT ELLQGFTVEV LRHQPADLLE FALQHFTRLQ QENERKGAAR
60 70 80 90 100
FGHEGRTWGD AGAAAGGGIP SKGVNFAEEP MRSDSENGEE EEAAEAGAFN
110 120 130 140 150
APVINRFTRR ASVCAEAYNP DEEEDDAESR IIHPKTDDQR NRLQEACKDI
160 170 180 190 200
LLFKNLDPEQ MSQVLDAMFE KLVKEGEHVI DQGDDGDNFY VIDRGTFDIY
210 220 230 240 250
VKCDGVGRCV GNYDNRGSFG ELALMYNTPR AATITATSPG ALWGLDRVTF
260 270 280 290 300
RRIIVKNNAK KRKMYESFIE SLPFLKSLEV SERLKVVDVI GTKVYNDGEQ
310 320 330 340 350
IIAQGDLADS FFIVESGEVK ITMKRKGKSE VEENGAVEIA RCFRGQYFGE
360 370 380 390 400
LALVTNKPRA ASAHAIGTVK CLAMDVQAFE RLLGPCMEIM KRNIATYEEQ
410
LVALFGTNMD IVEPTA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH3BK84 | H3BK84_MOUSE | cAMP-dependent protein kinase type ... | Prkar2b | 256 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 69 | I → T in AAH48710 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK041013 mRNA Translation: BAC30779.1 AK138963 mRNA Translation: BAE23838.1 CT010463 Genomic DNA No translation available. BC048710 mRNA Translation: AAH48710.1 M68861 Genomic DNA Translation: AAA40057.1 |
CCDSi | CCDS25869.1 |
PIRi | PQ0161 |
RefSeqi | NP_035288.2, NM_011158.3 XP_006515077.1, XM_006515014.3 |
Genome annotation databases
Ensembli | ENSMUST00000003079; ENSMUSP00000003079; ENSMUSG00000002997 ENSMUST00000036497; ENSMUSP00000039797; ENSMUSG00000002997 |
GeneIDi | 19088 |
KEGGi | mmu:19088 |
UCSCi | uc007nhx.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK041013 mRNA Translation: BAC30779.1 AK138963 mRNA Translation: BAE23838.1 CT010463 Genomic DNA No translation available. BC048710 mRNA Translation: AAH48710.1 M68861 Genomic DNA Translation: AAA40057.1 |
CCDSi | CCDS25869.1 |
PIRi | PQ0161 |
RefSeqi | NP_035288.2, NM_011158.3 XP_006515077.1, XM_006515014.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3TNP | X-ray | 2.30 | B/E | 1-416 | [»] | |
3TNQ | X-ray | 3.10 | A | 1-416 | [»] | |
4WBB | X-ray | 2.80 | A | 1-416 | [»] | |
4X6Q | X-ray | 2.52 | B | 1-416 | [»] | |
SMRi | P31324 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202369, 29 interactors |
CORUMi | P31324 |
DIPi | DIP-31571N |
IntActi | P31324, 18 interactors |
MINTi | P31324 |
STRINGi | 10090.ENSMUSP00000039797 |
PTM databases
iPTMneti | P31324 |
PhosphoSitePlusi | P31324 |
SwissPalmi | P31324 |
Proteomic databases
EPDi | P31324 |
jPOSTi | P31324 |
MaxQBi | P31324 |
PaxDbi | P31324 |
PeptideAtlasi | P31324 |
PRIDEi | P31324 |
ProteomicsDBi | 301730 |
Protocols and materials databases
Antibodypediai | 1082, 389 antibodies from 35 providers |
DNASUi | 19088 |
Genome annotation databases
Ensembli | ENSMUST00000003079; ENSMUSP00000003079; ENSMUSG00000002997 ENSMUST00000036497; ENSMUSP00000039797; ENSMUSG00000002997 |
GeneIDi | 19088 |
KEGGi | mmu:19088 |
UCSCi | uc007nhx.2, mouse |
Organism-specific databases
CTDi | 5577 |
MGIi | MGI:97760, Prkar2b |
VEuPathDBi | HostDB:ENSMUSG00000002997 |
Phylogenomic databases
eggNOGi | KOG1113, Eukaryota |
GeneTreei | ENSGT00940000158160 |
HOGENOMi | CLU_018310_2_0_1 |
InParanoidi | P31324 |
OMAi | QYNFGRR |
OrthoDBi | 1047290at2759 |
PhylomeDBi | P31324 |
TreeFami | TF314920 |
Enzyme and pathway databases
Reactomei | R-MMU-163615, PKA activation R-MMU-164378, PKA activation in glucagon signalling R-MMU-180024, DARPP-32 events R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-MMU-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-MMU-5610787, Hedgehog 'off' state R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-8854518, AURKA Activation by TPX2 R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
BioGRID-ORCSi | 19088, 0 hits in 64 CRISPR screens |
ChiTaRSi | Prkar2b, mouse |
PROi | PR:P31324 |
RNActi | P31324, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000002997, Expressed in female gonad and 271 other tissues |
ExpressionAtlasi | P31324, baseline and differential |
Genevisiblei | P31324, MM |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits |
DisProti | DP01944 |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR012198, cAMP_dep_PK_reg_su IPR003117, cAMP_dep_PK_reg_su_I/II_a/b IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF02197, RIIa, 1 hit |
PIRSFi | PIRSF000548, PK_regulatory, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00394, RIIa, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits |
PROSITEi | View protein in PROSITE PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KAP3_MOUSE | |
Accessioni | P31324Primary (citable) accession number: P31324 Secondary accession number(s): B1B199 Q8BRZ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 188 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families