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Protein

cAMP-dependent protein kinase type I-beta regulatory subunit

Gene

PRKAR1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei202cAMP 11
Binding sitei211cAMP 11
Binding sitei326cAMP 21
Binding sitei335cAMP 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 254cAMP 1Add BLAST118
Nucleotide bindingi255 – 381cAMP 2Add BLAST127

GO - Molecular functioni

  • cAMP binding Source: UniProtKB-KW
  • cAMP-dependent protein kinase inhibitor activity Source: BHF-UCL
  • cAMP-dependent protein kinase regulator activity Source: BHF-UCL
  • protein kinase A catalytic subunit binding Source: BHF-UCL

GO - Biological processi

Keywordsi

LigandcAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-163615 PKA activation
R-HSA-164378 PKA activation in glucagon signalling
R-HSA-180024 DARPP-32 events
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-5610787 Hedgehog 'off' state
R-HSA-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type I-beta regulatory subunit
Gene namesi
Name:PRKAR1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000188191.14
HGNCiHGNC:9390 PRKAR1B
MIMi176911 gene
neXtProtiNX_P31321

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5575
MalaCardsiPRKAR1B
OpenTargetsiENSG00000188191
PharmGKBiPA33756

Chemistry databases

GuidetoPHARMACOLOGYi1473

Polymorphism and mutation databases

BioMutaiPRKAR1B
DMDMi229463042

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002053812 – 381cAMP-dependent protein kinase type I-beta regulatory subunitAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineCombined sources1
Disulfide bondi18Interchain (with C-39)By similarity
Modified residuei21Nitrated tyrosineCombined sources1
Disulfide bondi39Interchain (with C-18)By similarity
Modified residuei77PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei85PhosphothreonineBy similarity1
Modified residuei97Omega-N-methylarginineBy similarity1

Post-translational modificationi

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.

Keywords - PTMi

Disulfide bond, Methylation, Nitration, Phosphoprotein

Proteomic databases

EPDiP31321
MaxQBiP31321
PaxDbiP31321
PeptideAtlasiP31321
PRIDEiP31321
ProteomicsDBi54780

PTM databases

iPTMnetiP31321
PhosphoSitePlusiP31321

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

BgeeiENSG00000188191 Expressed in 218 organ(s), highest expression level in anterior cingulate cortex
CleanExiHS_PRKAR1B
ExpressionAtlasiP31321 baseline and differential
GenevisibleiP31321 HS

Organism-specific databases

HPAiHPA026719

Interactioni

Subunit structurei

The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Interacts with C2orf88/smAKAP; this interaction may target PRKAR1B to the plasma membrane.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRKACAP176124EBI-2805516,EBI-476586

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111561, 50 interactors
IntActiP31321, 80 interactors
MINTiP31321
STRINGi9606.ENSP00000353415

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP31321
SMRiP31321
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 136Dimerization and phosphorylationAdd BLAST135

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 100Pseudophosphorylation motif5

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196669
HOVERGENiHBG002025
InParanoidiP31321
KOiK04739
OMAiDCIVHLC
OrthoDBiEOG091G0F1K
PhylomeDBiP31321
TreeFamiTF314920

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.iShow all

P31321-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASPPACPSE EDESLKGCEL YVQLHGIQQV LKDCIVHLCI SKPERPMKFL
60 70 80 90 100
REHFEKLEKE ENRQILARQK SNSQSDSHDE EVSPTPPNPV VKARRRRGGV
110 120 130 140 150
SAEVYTEEDA VSYVRKVIPK DYKTMTALAK AISKNVLFAH LDDNERSDIF
160 170 180 190 200
DAMFPVTHIA GETVIQQGNE GDNFYVVDQG EVDVYVNGEW VTNISEGGSF
210 220 230 240 250
GELALIYGTP RAATVKAKTD LKLWGIDRDS YRRILMGSTL RKRKMYEEFL
260 270 280 290 300
SKVSILESLE KWERLTVADA LEPVQFEDGE KIVVQGEPGD DFYIITEGTA
310 320 330 340 350
SVLQRRSPNE EYVEVGRLGP SDYFGEIALL LNRPRAATVV ARGPLKCVKL
360 370 380
DRPRFERVLG PCSEILKRNI QRYNSFISLT V
Length:381
Mass (Da):43,073
Last modified:May 5, 2009 - v4
Checksum:i1ED7BFEC30897191
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JSK5C9JSK5_HUMAN
cAMP-dependent protein kinase type ...
PRKAR1B
297Annotation score:
C9JR00C9JR00_HUMAN
cAMP-dependent protein kinase type ...
PRKAR1B
169Annotation score:
H7BYW5H7BYW5_HUMAN
cAMP-dependent protein kinase type ...
PRKAR1B
242Annotation score:
C9IZL8C9IZL8_HUMAN
cAMP-dependent protein kinase type ...
PRKAR1B
165Annotation score:
C9J4C2C9J4C2_HUMAN
cAMP-dependent protein kinase type ...
PRKAR1B
116Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti270A → R in AAC37564 (PubMed:1708242).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65066 mRNA Translation: AAC37564.1
AK315951 mRNA Translation: BAH14322.1
AK315990 mRNA Translation: BAH14361.1
BC026734 mRNA Translation: AAH26734.1
BC036828 mRNA Translation: AAH36828.2
CCDSiCCDS34579.1
PIRiJH0392 OKHUR1
RefSeqiNP_001158230.1, NM_001164758.1
NP_001158231.1, NM_001164759.1
NP_001158232.1, NM_001164760.1
NP_001158233.1, NM_001164761.1
NP_001158234.1, NM_001164762.1
NP_002726.1, NM_002735.2
UniGeneiHs.520851

Genome annotation databases

EnsembliENST00000360274; ENSP00000353415; ENSG00000188191
ENST00000403562; ENSP00000385349; ENSG00000188191
ENST00000406797; ENSP00000385749; ENSG00000188191
ENST00000537384; ENSP00000440449; ENSG00000188191
ENST00000544935; ENSP00000444487; ENSG00000188191
GeneIDi5575
KEGGihsa:5575
UCSCiuc003siu.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65066 mRNA Translation: AAC37564.1
AK315951 mRNA Translation: BAH14322.1
AK315990 mRNA Translation: BAH14361.1
BC026734 mRNA Translation: AAH26734.1
BC036828 mRNA Translation: AAH36828.2
CCDSiCCDS34579.1
PIRiJH0392 OKHUR1
RefSeqiNP_001158230.1, NM_001164758.1
NP_001158231.1, NM_001164759.1
NP_001158232.1, NM_001164760.1
NP_001158233.1, NM_001164761.1
NP_001158234.1, NM_001164762.1
NP_002726.1, NM_002735.2
UniGeneiHs.520851

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DINX-ray3.70B1-381[»]
4F9KX-ray2.80A/B/C/D11-73[»]
ProteinModelPortaliP31321
SMRiP31321
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111561, 50 interactors
IntActiP31321, 80 interactors
MINTiP31321
STRINGi9606.ENSP00000353415

Chemistry databases

GuidetoPHARMACOLOGYi1473

PTM databases

iPTMnetiP31321
PhosphoSitePlusiP31321

Polymorphism and mutation databases

BioMutaiPRKAR1B
DMDMi229463042

Proteomic databases

EPDiP31321
MaxQBiP31321
PaxDbiP31321
PeptideAtlasiP31321
PRIDEiP31321
ProteomicsDBi54780

Protocols and materials databases

DNASUi5575
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360274; ENSP00000353415; ENSG00000188191
ENST00000403562; ENSP00000385349; ENSG00000188191
ENST00000406797; ENSP00000385749; ENSG00000188191
ENST00000537384; ENSP00000440449; ENSG00000188191
ENST00000544935; ENSP00000444487; ENSG00000188191
GeneIDi5575
KEGGihsa:5575
UCSCiuc003siu.3 human

Organism-specific databases

CTDi5575
DisGeNETi5575
EuPathDBiHostDB:ENSG00000188191.14
GeneCardsiPRKAR1B
HGNCiHGNC:9390 PRKAR1B
HPAiHPA026719
MalaCardsiPRKAR1B
MIMi176911 gene
neXtProtiNX_P31321
OpenTargetsiENSG00000188191
PharmGKBiPA33756
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196669
HOVERGENiHBG002025
InParanoidiP31321
KOiK04739
OMAiDCIVHLC
OrthoDBiEOG091G0F1K
PhylomeDBiP31321
TreeFamiTF314920

Enzyme and pathway databases

ReactomeiR-HSA-163615 PKA activation
R-HSA-164378 PKA activation in glucagon signalling
R-HSA-180024 DARPP-32 events
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-5610787 Hedgehog 'off' state
R-HSA-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

ChiTaRSiPRKAR1B human
GeneWikiiPRKAR1B
GenomeRNAii5575
PROiPR:P31321
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000188191 Expressed in 218 organ(s), highest expression level in anterior cingulate cortex
CleanExiHS_PRKAR1B
ExpressionAtlasiP31321 baseline and differential
GenevisibleiP31321 HS

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiKAP1_HUMAN
AccessioniPrimary (citable) accession number: P31321
Secondary accession number(s): Q8N422
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 5, 2009
Last modified: September 12, 2018
This is version 177 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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