UniProtKB - P31321 (KAP1_HUMAN)
cAMP-dependent protein kinase type I-beta regulatory subunit
PRKAR1B
Functioni
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 202 | 3',5'-cAMP 1 | 1 | |
Binding sitei | 211 | 3',5'-cAMP 1 | 1 | |
Binding sitei | 326 | 3',5'-cAMP 2 | 1 | |
Binding sitei | 335 | 3',5'-cAMP 2 | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 137 – 254 | 3',5'-cAMP 1Add BLAST | 118 | |
Nucleotide bindingi | 255 – 381 | 3',5'-cAMP 2Add BLAST | 127 |
GO - Molecular functioni
- cAMP binding Source: GO_Central
- cAMP-dependent protein kinase inhibitor activity Source: BHF-UCL
- cAMP-dependent protein kinase regulator activity Source: BHF-UCL
- protein kinase A catalytic subunit binding Source: BHF-UCL
GO - Biological processi
- learning or memory Source: Ensembl
- negative regulation of cAMP-dependent protein kinase activity Source: BHF-UCL
- positive regulation of excitatory postsynaptic potential Source: Ensembl
- positive regulation of fear response Source: Ensembl
- positive regulation of long-term synaptic potentiation Source: Ensembl
- positive regulation of sensory perception of pain Source: Ensembl
- protein phosphorylation Source: UniProtKB
- regulation of synaptic vesicle cycle Source: Ensembl
Keywordsi
Ligand | cAMP, cAMP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P31321 |
Reactomei | R-HSA-163615, PKA activation R-HSA-164378, PKA activation in glucagon signalling R-HSA-180024, DARPP-32 events R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-HSA-5610787, Hedgehog 'off' state R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production R-HSA-9664323, FCGR3A-mediated IL10 synthesis R-HSA-983231, Factors involved in megakaryocyte development and platelet production |
SignaLinki | P31321 |
SIGNORi | P31321 |
Names & Taxonomyi
Protein namesi | Recommended name: cAMP-dependent protein kinase type I-beta regulatory subunit |
Gene namesi | Name:PRKAR1B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9390, PRKAR1B |
MIMi | 176911, gene |
neXtProti | NX_P31321 |
VEuPathDBi | HostDB:ENSG00000188191 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Cytosol
- cytosol Source: Reactome
Endosome
- multivesicular body Source: Ensembl
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cAMP-dependent protein kinase complex Source: GO_Central
- ciliary base Source: Reactome
- cytoplasm Source: GO_Central
- glutamatergic synapse Source: Ensembl
- hippocampal mossy fiber to CA3 synapse Source: Ensembl
- Schaffer collateral - CA1 synapse Source: Ensembl
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 5575 |
MalaCardsi | PRKAR1B |
OpenTargetsi | ENSG00000188191 |
Orphaneti | 412066, PRKAR1B-related neurodegenerative dementia with intermediate filaments |
PharmGKBi | PA33756 |
Miscellaneous databases
Pharosi | P31321, Tbio |
Chemistry databases
ChEMBLi | CHEMBL2320 |
GuidetoPHARMACOLOGYi | 1473 |
Genetic variation databases
BioMutai | PRKAR1B |
DMDMi | 229463042 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000205381 | 2 – 381 | cAMP-dependent protein kinase type I-beta regulatory subunitAdd BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | PhosphoserineCombined sources | 1 | |
Disulfide bondi | 18 | Interchain (with C-39)By similarity | ||
Modified residuei | 21 | 3'-nitrotyrosineCombined sources | 1 | |
Disulfide bondi | 39 | Interchain (with C-18)By similarity | ||
Modified residuei | 77 | PhosphoserineCombined sources | 1 | |
Modified residuei | 83 | PhosphoserineCombined sources | 1 | |
Modified residuei | 85 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 97 | Omega-N-methylarginineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Methylation, Nitration, PhosphoproteinProteomic databases
EPDi | P31321 |
jPOSTi | P31321 |
MassIVEi | P31321 |
MaxQBi | P31321 |
PaxDbi | P31321 |
PeptideAtlasi | P31321 |
PRIDEi | P31321 |
ProteomicsDBi | 54780 |
PTM databases
iPTMneti | P31321 |
PhosphoSitePlusi | P31321 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000188191, Expressed in anterior cingulate cortex and 231 other tissues |
ExpressionAtlasi | P31321, baseline and differential |
Genevisiblei | P31321, HS |
Organism-specific databases
HPAi | ENSG00000188191, Tissue enriched (brain) |
Interactioni
Subunit structurei
The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer.
Interacts with PRKX; regulates this cAMP-dependent protein kinase.
Interacts with C2orf88/smAKAP; this interaction may target PRKAR1B to the plasma membrane.
2 PublicationsBinary interactionsi
P31321
GO - Molecular functioni
- protein kinase A catalytic subunit binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 111561, 167 interactors |
IntActi | P31321, 101 interactors |
MINTi | P31321 |
STRINGi | 9606.ENSP00000385749 |
Miscellaneous databases
RNActi | P31321, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P31321 |
SMRi | P31321 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 136 | Dimerization and phosphorylationAdd BLAST | 135 | |
Regioni | 67 – 98 | DisorderedSequence analysisAdd BLAST | 32 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 96 – 100 | Pseudophosphorylation motif | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 67 – 82 | Polar residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1113, Eukaryota |
GeneTreei | ENSGT00940000157513 |
HOGENOMi | CLU_018310_1_1_1 |
InParanoidi | P31321 |
OMAi | LYVQKHC |
OrthoDBi | 1047290at2759 |
PhylomeDBi | P31321 |
TreeFami | TF314920 |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits cd12102, DD_RIbeta_PKA, 1 hit |
DisProti | DP02632 |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR012198, cAMP_dep_PK_reg_su IPR003117, cAMP_dep_PK_reg_su_I/II_a/b IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR042818, RIbeta_DD IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF02197, RIIa, 1 hit |
PIRSFi | PIRSF000548, PK_regulatory, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00394, RIIa, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits |
PROSITEi | View protein in PROSITE PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MASPPACPSE EDESLKGCEL YVQLHGIQQV LKDCIVHLCI SKPERPMKFL
60 70 80 90 100
REHFEKLEKE ENRQILARQK SNSQSDSHDE EVSPTPPNPV VKARRRRGGV
110 120 130 140 150
SAEVYTEEDA VSYVRKVIPK DYKTMTALAK AISKNVLFAH LDDNERSDIF
160 170 180 190 200
DAMFPVTHIA GETVIQQGNE GDNFYVVDQG EVDVYVNGEW VTNISEGGSF
210 220 230 240 250
GELALIYGTP RAATVKAKTD LKLWGIDRDS YRRILMGSTL RKRKMYEEFL
260 270 280 290 300
SKVSILESLE KWERLTVADA LEPVQFEDGE KIVVQGEPGD DFYIITEGTA
310 320 330 340 350
SVLQRRSPNE EYVEVGRLGP SDYFGEIALL LNRPRAATVV ARGPLKCVKL
360 370 380
DRPRFERVLG PCSEILKRNI QRYNSFISLT V
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JSK5 | C9JSK5_HUMAN | cAMP-dependent protein kinase type ... | PRKAR1B | 297 | Annotation score: | ||
C9IZL8 | C9IZL8_HUMAN | cAMP-dependent protein kinase type ... | PRKAR1B | 165 | Annotation score: | ||
C9JR00 | C9JR00_HUMAN | cAMP-dependent protein kinase type ... | PRKAR1B | 169 | Annotation score: | ||
C9J4C2 | C9J4C2_HUMAN | cAMP-dependent protein kinase type ... | PRKAR1B | 116 | Annotation score: | ||
H7BYW5 | H7BYW5_HUMAN | cAMP-dependent protein kinase type ... | PRKAR1B | 242 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 270 | A → R in AAC37564 (PubMed:1708242).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65066 mRNA Translation: AAC37564.1 AK315951 mRNA Translation: BAH14322.1 AK315990 mRNA Translation: BAH14361.1 BC026734 mRNA Translation: AAH26734.1 BC036828 mRNA Translation: AAH36828.2 |
CCDSi | CCDS34579.1 |
PIRi | JH0392, OKHUR1 |
RefSeqi | NP_001158230.1, NM_001164758.1 NP_001158231.1, NM_001164759.1 NP_001158232.1, NM_001164760.1 NP_001158233.1, NM_001164761.1 NP_001158234.1, NM_001164762.1 NP_002726.1, NM_002735.2 |
Genome annotation databases
Ensembli | ENST00000360274.8; ENSP00000353415.4; ENSG00000188191.15 ENST00000403562.5; ENSP00000385349.1; ENSG00000188191.15 ENST00000406797.5; ENSP00000385749.1; ENSG00000188191.15 ENST00000537384.6; ENSP00000440449.1; ENSG00000188191.15 ENST00000544935.5; ENSP00000444487.1; ENSG00000188191.15 |
GeneIDi | 5575 |
KEGGi | hsa:5575 |
MANE-Selecti | ENST00000537384.6; ENSP00000440449.1; NM_001164760.2; NP_001158232.1 |
UCSCi | uc003siu.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65066 mRNA Translation: AAC37564.1 AK315951 mRNA Translation: BAH14322.1 AK315990 mRNA Translation: BAH14361.1 BC026734 mRNA Translation: AAH26734.1 BC036828 mRNA Translation: AAH36828.2 |
CCDSi | CCDS34579.1 |
PIRi | JH0392, OKHUR1 |
RefSeqi | NP_001158230.1, NM_001164758.1 NP_001158231.1, NM_001164759.1 NP_001158232.1, NM_001164760.1 NP_001158233.1, NM_001164761.1 NP_001158234.1, NM_001164762.1 NP_002726.1, NM_002735.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4DIN | X-ray | 3.70 | B | 1-381 | [»] | |
4F9K | X-ray | 2.80 | A/B/C/D | 11-73 | [»] | |
AlphaFoldDBi | P31321 | |||||
SMRi | P31321 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111561, 167 interactors |
IntActi | P31321, 101 interactors |
MINTi | P31321 |
STRINGi | 9606.ENSP00000385749 |
Chemistry databases
ChEMBLi | CHEMBL2320 |
GuidetoPHARMACOLOGYi | 1473 |
PTM databases
iPTMneti | P31321 |
PhosphoSitePlusi | P31321 |
Genetic variation databases
BioMutai | PRKAR1B |
DMDMi | 229463042 |
Proteomic databases
EPDi | P31321 |
jPOSTi | P31321 |
MassIVEi | P31321 |
MaxQBi | P31321 |
PaxDbi | P31321 |
PeptideAtlasi | P31321 |
PRIDEi | P31321 |
ProteomicsDBi | 54780 |
Protocols and materials databases
Antibodypediai | 10769, 357 antibodies from 32 providers |
DNASUi | 5575 |
Genome annotation databases
Organism-specific databases
CTDi | 5575 |
DisGeNETi | 5575 |
GeneCardsi | PRKAR1B |
HGNCi | HGNC:9390, PRKAR1B |
HPAi | ENSG00000188191, Tissue enriched (brain) |
MalaCardsi | PRKAR1B |
MIMi | 176911, gene |
neXtProti | NX_P31321 |
OpenTargetsi | ENSG00000188191 |
Orphaneti | 412066, PRKAR1B-related neurodegenerative dementia with intermediate filaments |
PharmGKBi | PA33756 |
VEuPathDBi | HostDB:ENSG00000188191 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1113, Eukaryota |
GeneTreei | ENSGT00940000157513 |
HOGENOMi | CLU_018310_1_1_1 |
InParanoidi | P31321 |
OMAi | LYVQKHC |
OrthoDBi | 1047290at2759 |
PhylomeDBi | P31321 |
TreeFami | TF314920 |
Enzyme and pathway databases
PathwayCommonsi | P31321 |
Reactomei | R-HSA-163615, PKA activation R-HSA-164378, PKA activation in glucagon signalling R-HSA-180024, DARPP-32 events R-HSA-381676, Glucagon-like Peptide-1 (GLP1) regulates insulin secretion R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-442720, CREB1 phosphorylation through the activation of Adenylate Cyclase R-HSA-5610787, Hedgehog 'off' state R-HSA-9660821, ADORA2B mediated anti-inflammatory cytokines production R-HSA-9664323, FCGR3A-mediated IL10 synthesis R-HSA-983231, Factors involved in megakaryocyte development and platelet production |
SignaLinki | P31321 |
SIGNORi | P31321 |
Miscellaneous databases
BioGRID-ORCSi | 5575, 21 hits in 1080 CRISPR screens |
ChiTaRSi | PRKAR1B, human |
GeneWikii | PRKAR1B |
GenomeRNAii | 5575 |
Pharosi | P31321, Tbio |
PROi | PR:P31321 |
RNActi | P31321, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000188191, Expressed in anterior cingulate cortex and 231 other tissues |
ExpressionAtlasi | P31321, baseline and differential |
Genevisiblei | P31321, HS |
Family and domain databases
CDDi | cd00038, CAP_ED, 2 hits cd12102, DD_RIbeta_PKA, 1 hit |
DisProti | DP02632 |
Gene3Di | 2.60.120.10, 2 hits |
InterProi | View protein in InterPro IPR012198, cAMP_dep_PK_reg_su IPR003117, cAMP_dep_PK_reg_su_I/II_a/b IPR018490, cNMP-bd-like IPR018488, cNMP-bd_CS IPR000595, cNMP-bd_dom IPR042818, RIbeta_DD IPR014710, RmlC-like_jellyroll |
Pfami | View protein in Pfam PF00027, cNMP_binding, 2 hits PF02197, RIIa, 1 hit |
PIRSFi | PIRSF000548, PK_regulatory, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 2 hits SM00394, RIIa, 1 hit |
SUPFAMi | SSF51206, SSF51206, 2 hits |
PROSITEi | View protein in PROSITE PS00888, CNMP_BINDING_1, 2 hits PS00889, CNMP_BINDING_2, 2 hits PS50042, CNMP_BINDING_3, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | KAP1_HUMAN | |
Accessioni | P31321Primary (citable) accession number: P31321 Secondary accession number(s): Q8N422 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | May 5, 2009 | |
Last modified: | May 25, 2022 | |
This is version 200 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 7
Human chromosome 7: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families