UniProtKB - P31285 (WNT3A_XENLA)
Protein
Protein Wnt-3a
Gene
wnt3a
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube.By similarity
GO - Molecular functioni
- signaling receptor binding Source: InterPro
GO - Biological processi
- canonical Wnt signaling pathway Source: BHF-UCL
- neural crest cell differentiation Source: BHF-UCL
- positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
Keywordsi
Molecular function | Developmental protein |
Biological process | Wnt signaling pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Protein Wnt-3aShort name: XWnt-3a |
Gene namesi | Name:wnt3a |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-1217290, wnt3a.S |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: UniProtKB
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000041420 | 19 – 352 | Protein Wnt-3aAdd BLAST | 334 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 77 ↔ 88 | By similarity | ||
Glycosylationi | 87 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 128 ↔ 136 | By similarity | ||
Disulfide bondi | 138 ↔ 155 | By similarity | ||
Disulfide bondi | 203 ↔ 217 | By similarity | ||
Disulfide bondi | 205 ↔ 212 | By similarity | ||
Lipidationi | 209 | O-palmitoleoyl serineBy similarity | 1 | |
Disulfide bondi | 297 ↔ 312 | By similarity | ||
Glycosylationi | 298 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 327 ↔ 342 | By similarity | ||
Disulfide bondi | 329 ↔ 339 | By similarity | ||
Disulfide bondi | 334 ↔ 335 | By similarity |
Post-translational modificationi
Disulfide bonds have critical and distinct roles in secretion and activity. Loss of each conserved cysteine results in high molecular weight oxidized Wnt oligomers, which are formed through inter-Wnt disulfide bonding.By similarity
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, LipoproteinProteomic databases
PRIDEi | P31285 |
Expressioni
Tissue specificityi
At neurula in anterior neural fold; at tailbud in dorsal midline of midbrain.
Developmental stagei
Neurula onwards.
Interactioni
Binary interactionsi
P31285
With | #Exp. | IntAct |
---|---|---|
ptk7 [Q6PA07] | 2 | EBI-7036309,EBI-7036323 |
GO - Molecular functioni
- signaling receptor binding Source: InterPro
Protein-protein interaction databases
IntActi | P31285, 3 interactors |
MINTi | P31285 |
Family & Domainsi
Sequence similaritiesi
Belongs to the Wnt family.Curated
Keywords - Domaini
SignalPhylogenomic databases
OrthoDBi | 965867at2759 |
Family and domain databases
Gene3Di | 3.30.2460.20, 1 hit |
InterProi | View protein in InterPro IPR005817, Wnt IPR009141, Wnt3 IPR043158, Wnt_C IPR018161, Wnt_CS |
PANTHERi | PTHR12027, PTHR12027, 1 hit |
Pfami | View protein in Pfam PF00110, wnt, 1 hit |
PRINTSi | PR01843, WNT3PROTEIN PR01349, WNTPROTEIN |
SMARTi | View protein in SMART SM00097, WNT1, 1 hit |
PROSITEi | View protein in PROSITE PS00246, WNT1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P31285-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGCFGYLLLI IGLHQVLATY PIWWSLAVGQ QYSSLGTQPI PCGTIPGLVA
60 70 80 90 100
KQMRFCRNYM EIMPSVAEGV KIGIQECQHQ FRGRRWNCTT VNDNLAIFGP
110 120 130 140 150
VLDKATRESA FVHAIASAGV AFAVTRSCAE GSATICGCDT HHKGPPGEGW
160 170 180 190 200
KWGGCSEDMD FGSMVSREFA DARENRPDAR SAMNRHNNEA GRTSILDHRH
210 220 230 240 250
LKCKCHGLSG SCEVKTCWWS QPDFRVIGDY LKDKYDSASE MVVEKHRESR
260 270 280 290 300
GWVETLRPKY TFFKPPIERD LIYYESSPNF CEPNPETGSF GTRDRECNVT
310 320 330 340 350
SHGIDGCDLL CCGRGQNTRT EKRKEKCHCI FHWCCYVSCQ ECMRVYDVHT
CK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07538 mRNA Translation: AAA50009.1 |
PIRi | A48828 |
RefSeqi | NP_001079343.1, NM_001085874.1 |
Genome annotation databases
GeneIDi | 378679 |
KEGGi | xla:378679 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07538 mRNA Translation: AAA50009.1 |
PIRi | A48828 |
RefSeqi | NP_001079343.1, NM_001085874.1 |
3D structure databases
SMRi | P31285 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P31285, 3 interactors |
MINTi | P31285 |
Proteomic databases
PRIDEi | P31285 |
Genome annotation databases
GeneIDi | 378679 |
KEGGi | xla:378679 |
Organism-specific databases
CTDi | 378679 |
Xenbasei | XB-GENE-1217290, wnt3a.S |
Phylogenomic databases
OrthoDBi | 965867at2759 |
Family and domain databases
Gene3Di | 3.30.2460.20, 1 hit |
InterProi | View protein in InterPro IPR005817, Wnt IPR009141, Wnt3 IPR043158, Wnt_C IPR018161, Wnt_CS |
PANTHERi | PTHR12027, PTHR12027, 1 hit |
Pfami | View protein in Pfam PF00110, wnt, 1 hit |
PRINTSi | PR01843, WNT3PROTEIN PR01349, WNTPROTEIN |
SMARTi | View protein in SMART SM00097, WNT1, 1 hit |
PROSITEi | View protein in PROSITE PS00246, WNT1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | WNT3A_XENLA | |
Accessioni | P31285Primary (citable) accession number: P31285 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 103 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |