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Protein

Recombining binding protein suppressor of hairless

Gene

Rbpj

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations (PubMed:7566092). Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen) (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (PubMed:26194095).By similarity2 Publications

Caution

Despite some similarity with the "phage" integrase family, it has no recombinase activity.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi84 – 91By similarity8
DNA bindingi218 – 22710
DNA bindingi291 – 323Add BLAST33

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processNotch signaling pathway, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-MMU-2197563 NOTCH2 intracellular domain regulates transcription
R-MMU-350054 Notch-HLH transcription pathway
R-MMU-8941856 RUNX3 regulates NOTCH signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Recombining binding protein suppressor of hairless
Alternative name(s):
J kappa-recombination signal-binding protein
RBP-J kappa
Gene namesi
Name:RbpjImported
Synonyms:Igkjrb1, Igkrsbp, Rbpsuh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:96522 Rbpj

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085681 – 526Recombining binding protein suppressor of hairlessAdd BLAST526

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP31266
PaxDbiP31266
PeptideAtlasiP31266
PRIDEiP31266

PTM databases

iPTMnetiP31266
PhosphoSitePlusiP31266
SwissPalmiP31266

Expressioni

Gene expression databases

BgeeiENSMUSG00000039191 Expressed in 293 organ(s), highest expression level in ureter smooth muscle
ExpressionAtlasiP31266 baseline and differential
GenevisibleiP31266 MM

Interactioni

Subunit structurei

Interacts with RITA1, leading to nuclear export, prevent the interaction between RBPJ and NICD product and subsequent down-regulation of the Notch signaling pathway (By similarity). Interacts with activated NOTCH1, NOTCH2 and NOTCH3. Interacts with MINT/SHARP. This interaction may mediate the recruitment of large corepressor complexes containing proteins such as HDAC1, HDAC2, NCOR2, SAP30, FHL1/KYOT2 and CIR1. Interacts with EP300, MAML1 and PTF1A. Interacts with SNW1. Interacts with CHCHD2 and CXXC5. Interacts with BEND6 (via BEN domain) (By similarity). Interacts with NKAPL (PubMed:25875095). Interacts with ZMIZ1 (By similarity). Interacts with RBM15 (PubMed:17283045).By similarity7 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202832, 9 interactors
CORUMiP31266
DIPiDIP-171N
ELMiP31266
IntActiP31266, 7 interactors
MINTiP31266
STRINGi10090.ENSMUSP00000040694

Structurei

Secondary structure

1526
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP31266
SMRiP31266
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31266

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini381 – 471IPT/TIGAdd BLAST91

Sequence similaritiesi

Belongs to the Su(H) family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3743 Eukaryota
ENOG410XV7K LUCA
GeneTreeiENSGT00390000005197
HOGENOMiHOG000253907
HOVERGENiHBG006618
InParanoidiP31266
KOiK06053
OMAiDMPHFGL
OrthoDBiEOG091G057S
PhylomeDBiP31266
TreeFamiTF314117

Family and domain databases

CDDicd01176 IPT_RBP-Jkappa, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.1450, 2 hits
InterProiView protein in InterPro
IPR015350 Beta-trefoil_DNA-bd_dom
IPR036358 BTD_sf
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR008967 p53-like_TF_DNA-bd
IPR015351 RBP-J/Cbf11/Cbf12_DNA-bd
IPR037095 RBP-J/Cbf11/Cbf12_DNA-bd_sf
IPR038007 RBP-Jkappa_IPT
PfamiView protein in Pfam
PF09270 BTD, 1 hit
PF09271 LAG1-DNAbind, 1 hit
SMARTiView protein in SMART
SM01268 BTD, 1 hit
SM01267 LAG1_DNAbind, 1 hit
SUPFAMiSSF110217 SSF110217, 1 hit
SSF49417 SSF49417, 1 hit
SSF81296 SSF81296, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P31266-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPSGFPQSPR TSPRARPKTR ITGALPMDYS EGLSAEERPA HAPSAGKFGE
60 70 80 90 100
RPPPKRLTRE AMRNYLKERG DQTVLILHAK VAQKSYGNEK RFFCPPPCVY
110 120 130 140 150
LMGSGWKKKK EQMERDGCSE QESQPCAFIG IGNSDQEMQQ LNLEGKNYCT
160 170 180 190 200
AKTLYISDSD KRKHFMLSVK MFYGNSDDIG VFLSKRIKVI SKPSKKKQSL
210 220 230 240 250
KNADLCIASG TKVALFNRLR SQTVSTRYLH VEGGNFHASS QQWGAFYIHL
260 270 280 290 300
LDDDESEGEE FTVRDGYIHY GQTVKLVCSV TGMALPRLII RKVDKQTALL
310 320 330 340 350
DADDPVSQLH KCAFYLKDTE RMYLCLSQER IIQFQATPCP KEQNKEMIND
360 370 380 390 400
GASWTIISTD KAEYTFYEGM GPVLAPVTPV PVVESLQLNG GGDVAMLELT
410 420 430 440 450
GQNFTPNLRV WFGDVEAETM YRCGESMLCV VPDISAFREG WRWVRQPVQV
460 470 480 490 500
PVTLVRNDGV IYSTSLTFTY TPEPGPRPHC SAAGAILRAN SSQVPSNESN
510 520
TNSEGNYTNA STNSTSVTSS TATVVS
Length:526
Mass (Da):58,537
Last modified:July 1, 1993 - v1
Checksum:i8BF517CC24099E03
GO
Isoform 2 (identifier: P31266-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.
     40-45: AHAPSA → MAPVVT

Note: No experimental confirmation available.
Show »
Length:487
Mass (Da):54,347
Checksum:i88C0D1DCECACC9E2
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3U6F1Q3U6F1_MOUSE
Recombining-binding protein suppres...
Rbpj Rbpsuh
465Annotation score:
A0A0J9YTV5A0A0J9YTV5_MOUSE
Recombining binding protein suppres...
Rbpj Rbpsuh, mCG_16305
507Annotation score:
E9Q7W0E9Q7W0_MOUSE
Recombining-binding protein suppres...
Rbpj
485Annotation score:
A0A0J9YVE0A0A0J9YVE0_MOUSE
Recombining-binding protein suppres...
Rbpj
180Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0083921 – 39Missing in isoform 2. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_00839340 – 45AHAPSA → MAPVVT in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63463 mRNA Translation: AAB20195.1
X17459 mRNA Translation: CAA35501.1
X58337 Genomic DNA No translation available.
M81867, M81865, M81866 Genomic DNA Translation: AAA39018.1
M81868 Genomic DNA Translation: AAA39019.1
M81870, M81869 Genomic DNA Translation: AAA39020.1 Sequence problems.
M81873, M81872 Genomic DNA Translation: AAA39021.1
M81875, M81874 Genomic DNA Translation: AAA39022.1
M81876 Genomic DNA Translation: AAA39023.1
M81877 Genomic DNA No translation available.
BC051387 mRNA Translation: AAH51387.1
AK080359 mRNA Translation: BAC37889.1
CCDSiCCDS19292.1 [P31266-1]
CCDS51504.1 [P31266-2]
PIRiA42267
A43567
RefSeqiNP_001074396.1, NM_001080927.2 [P31266-2]
NP_033061.3, NM_009035.5 [P31266-1]
UniGeneiMm.209292
Mm.488709

Genome annotation databases

EnsembliENSMUST00000037618; ENSMUSP00000040694; ENSMUSG00000039191 [P31266-1]
ENSMUST00000113865; ENSMUSP00000109496; ENSMUSG00000039191 [P31266-2]
GeneIDi19664
KEGGimmu:19664
UCSCiuc008xli.2 mouse [P31266-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63463 mRNA Translation: AAB20195.1
X17459 mRNA Translation: CAA35501.1
X58337 Genomic DNA No translation available.
M81867, M81865, M81866 Genomic DNA Translation: AAA39018.1
M81868 Genomic DNA Translation: AAA39019.1
M81870, M81869 Genomic DNA Translation: AAA39020.1 Sequence problems.
M81873, M81872 Genomic DNA Translation: AAA39021.1
M81875, M81874 Genomic DNA Translation: AAA39022.1
M81876 Genomic DNA Translation: AAA39023.1
M81877 Genomic DNA No translation available.
BC051387 mRNA Translation: AAH51387.1
AK080359 mRNA Translation: BAC37889.1
CCDSiCCDS19292.1 [P31266-1]
CCDS51504.1 [P31266-2]
PIRiA42267
A43567
RefSeqiNP_001074396.1, NM_001080927.2 [P31266-2]
NP_033061.3, NM_009035.5 [P31266-1]
UniGeneiMm.209292
Mm.488709

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BRGX-ray2.20C53-474[»]
3IAGX-ray2.00C53-474[»]
4J2XX-ray2.85A/C53-474[»]
5EG6X-ray2.09C53-474[»]
ProteinModelPortaliP31266
SMRiP31266
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202832, 9 interactors
CORUMiP31266
DIPiDIP-171N
ELMiP31266
IntActiP31266, 7 interactors
MINTiP31266
STRINGi10090.ENSMUSP00000040694

PTM databases

iPTMnetiP31266
PhosphoSitePlusiP31266
SwissPalmiP31266

Proteomic databases

MaxQBiP31266
PaxDbiP31266
PeptideAtlasiP31266
PRIDEiP31266

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037618; ENSMUSP00000040694; ENSMUSG00000039191 [P31266-1]
ENSMUST00000113865; ENSMUSP00000109496; ENSMUSG00000039191 [P31266-2]
GeneIDi19664
KEGGimmu:19664
UCSCiuc008xli.2 mouse [P31266-1]

Organism-specific databases

CTDi3516
MGIiMGI:96522 Rbpj

Phylogenomic databases

eggNOGiKOG3743 Eukaryota
ENOG410XV7K LUCA
GeneTreeiENSGT00390000005197
HOGENOMiHOG000253907
HOVERGENiHBG006618
InParanoidiP31266
KOiK06053
OMAiDMPHFGL
OrthoDBiEOG091G057S
PhylomeDBiP31266
TreeFamiTF314117

Enzyme and pathway databases

ReactomeiR-MMU-2122947 NOTCH1 Intracellular Domain Regulates Transcription
R-MMU-2197563 NOTCH2 intracellular domain regulates transcription
R-MMU-350054 Notch-HLH transcription pathway
R-MMU-8941856 RUNX3 regulates NOTCH signaling

Miscellaneous databases

ChiTaRSiRbpj mouse
EvolutionaryTraceiP31266
PROiPR:P31266
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039191 Expressed in 293 organ(s), highest expression level in ureter smooth muscle
ExpressionAtlasiP31266 baseline and differential
GenevisibleiP31266 MM

Family and domain databases

CDDicd01176 IPT_RBP-Jkappa, 1 hit
Gene3Di2.60.40.10, 1 hit
2.60.40.1450, 2 hits
InterProiView protein in InterPro
IPR015350 Beta-trefoil_DNA-bd_dom
IPR036358 BTD_sf
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR008967 p53-like_TF_DNA-bd
IPR015351 RBP-J/Cbf11/Cbf12_DNA-bd
IPR037095 RBP-J/Cbf11/Cbf12_DNA-bd_sf
IPR038007 RBP-Jkappa_IPT
PfamiView protein in Pfam
PF09270 BTD, 1 hit
PF09271 LAG1-DNAbind, 1 hit
SMARTiView protein in SMART
SM01268 BTD, 1 hit
SM01267 LAG1_DNAbind, 1 hit
SUPFAMiSSF110217 SSF110217, 1 hit
SSF49417 SSF49417, 1 hit
SSF81296 SSF81296, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSUH_MOUSE
AccessioniPrimary (citable) accession number: P31266
Secondary accession number(s): Q80UN8, Q8C4Z3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 12, 2018
This is version 189 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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