UniProtKB - P31224 (ACRB_ECOLI)
Protein
Multidrug efflux pump subunit AcrB
Gene
acrB
Organism
Escherichia coli (strain K12)
Status
Functioni
AcrA-AcrB-AcrZ-TolC is a drug efflux protein complex with broad substrate specificity that uses the proton motive force to export substrates.4 Publications
(Microbial infection) Involved in contact-dependent growth inhibition (CDI), acts downstream of BamA, the receptor for CDI. Its role in CDI is independent of the AcrA-AcrB-TolC efflux pump complex.1 Publication
GO - Molecular functioni
- efflux transmembrane transporter activity Source: InterPro
- identical protein binding Source: IntAct
- xenobiotic transmembrane transporter activity Source: EcoliWiki
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:ACRB-MONOMER MetaCyc:ACRB-MONOMER |
Protein family/group databases
TCDBi | 2.A.6.2.2, the resistance-nodulation-cell division (rnd) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Multidrug efflux pump subunit AcrBAlternative name(s): AcrAB-TolC multidrug efflux pump subunit AcrB Acridine resistance protein B |
Gene namesi | Name:acrB Synonyms:acrE Ordered Locus Names:b0462, JW0451 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 2 Publications; Multi-pass membrane protein 2 Publications
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | Cytoplasmic1 Publication | 9 | |
Transmembranei | 10 – 28 | Helical; Name=1Add BLAST | 19 | |
Topological domaini | 29 – 336 | Periplasmic1 PublicationAdd BLAST | 308 | |
Transmembranei | 337 – 356 | Helical; Name=2Add BLAST | 20 | |
Topological domaini | 357 – 365 | Cytoplasmic1 Publication | 9 | |
Transmembranei | 366 – 385 | Helical; Name=3Add BLAST | 20 | |
Topological domaini | 386 – 391 | Periplasmic1 Publication | 6 | |
Transmembranei | 392 – 413 | Helical; Name=4Add BLAST | 22 | |
Topological domaini | 414 – 438 | Cytoplasmic1 PublicationAdd BLAST | 25 | |
Transmembranei | 439 – 457 | Helical; Name=5Add BLAST | 19 | |
Topological domaini | 458 – 465 | Periplasmic1 Publication | 8 | |
Transmembranei | 466 – 490 | Helical; Name=6Add BLAST | 25 | |
Topological domaini | 491 – 538 | Cytoplasmic1 PublicationAdd BLAST | 48 | |
Transmembranei | 539 – 555 | Helical; Name=7Add BLAST | 17 | |
Topological domaini | 556 – 871 | Periplasmic1 PublicationAdd BLAST | 316 | |
Transmembranei | 872 – 888 | Helical; Name=8Add BLAST | 17 | |
Topological domaini | 889 – 898 | Cytoplasmic1 Publication | 10 | |
Transmembranei | 899 – 918 | Helical; Name=9Add BLAST | 20 | |
Topological domaini | 919 – 924 | Periplasmic1 Publication | 6 | |
Transmembranei | 925 – 943 | Helical; Name=10Add BLAST | 19 | |
Topological domaini | 944 – 972 | Cytoplasmic1 PublicationAdd BLAST | 29 | |
Transmembranei | 973 – 992 | Helical; Name=11Add BLAST | 20 | |
Topological domaini | 993 – 998 | Periplasmic1 Publication | 6 | |
Transmembranei | 999 – 1018 | Helical; Name=12Add BLAST | 20 | |
Topological domaini | 1019 – 1049 | Cytoplasmic1 PublicationAdd BLAST | 31 |
GO - Cellular componenti
- efflux pump complex Source: EcoCyc
- integral component of plasma membrane Source: EcoCyc
- membrane Source: UniProtKB
- outer membrane-bounded periplasmic space Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Loss of susceptibility to contact-dependent growth inhibition (CDI); inhibiting cells still contact the target.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 526 | H → Y: Partially restores chloramphenicol resistance to an AcrZ G30R mutant. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL1681614 |
DrugBanki | DB03619, Deoxycholic acid DB04209, Dequalinium DB03825, Rhodamine 6G |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000161811 | 1 – 1049 | Multidrug efflux pump subunit AcrBAdd BLAST | 1049 |
Proteomic databases
jPOSTi | P31224 |
PaxDbi | P31224 |
PRIDEi | P31224 |
Expressioni
Inductioni
Positively regulated by MarA, Rob and SoxS transcriptional regulators (at protein level).1 Publication
Interactioni
Subunit structurei
Homotrimer, with large domains that extend into the periplasm, interacts with AcrA and TolC. AcrA may be required to stably link this protein and TolC.
Interacts with AcrZ. Part of the AcrA-AcrB-AcrZ-TolC efflux pump.
9 PublicationsBinary interactionsi
Hide detailsP31224
With | #Exp. | IntAct |
---|---|---|
itself | 9 | EBI-551006,EBI-551006 |
acrZ [P0AAW9] | 7 | EBI-551006,EBI-6313593 |
yajC [P0ADZ7] | 2 | EBI-551006,EBI-1130723 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259859, 386 interactors |
ComplexPortali | CPX-4263, AcrAB-TolC multidrug efflux transport complex |
DIPi | DIP-9049N |
IntActi | P31224, 9 interactors |
MINTi | P31224 |
STRINGi | 511145.b0462 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
PCDDBi | P31224 |
SMRi | P31224 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P31224 |
Family & Domainsi
Sequence similaritiesi
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0841, Bacteria |
HOGENOMi | CLU_002755_0_0_6 |
InParanoidi | P31224 |
PhylomeDBi | P31224 |
Family and domain databases
Gene3Di | 3.30.2090.10, 2 hits |
InterProi | View protein in InterPro IPR027463, AcrB_DN_DC_subdom IPR001036, Acrflvin-R IPR004764, HAE1 |
PANTHERi | PTHR32063, PTHR32063, 1 hit |
Pfami | View protein in Pfam PF00873, ACR_tran, 1 hit |
PRINTSi | PR00702, ACRIFLAVINRP |
SUPFAMi | SSF82714, SSF82714, 2 hits |
TIGRFAMsi | TIGR00915, 2A0602, 1 hit |
i Sequence
Sequence statusi: Complete.
P31224-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPNFFIDRPI FAWVIAIIIM LAGGLAILKL PVAQYPTIAP PAVTISASYP
60 70 80 90 100
GADAKTVQDT VTQVIEQNMN GIDNLMYMSS NSDSTGTVQI TLTFESGTDA
110 120 130 140 150
DIAQVQVQNK LQLAMPLLPQ EVQQQGVSVE KSSSSFLMVV GVINTDGTMT
160 170 180 190 200
QEDISDYVAA NMKDAISRTS GVGDVQLFGS QYAMRIWMNP NELNKFQLTP
210 220 230 240 250
VDVITAIKAQ NAQVAAGQLG GTPPVKGQQL NASIIAQTRL TSTEEFGKIL
260 270 280 290 300
LKVNQDGSRV LLRDVAKIEL GGENYDIIAE FNGQPASGLG IKLATGANAL
310 320 330 340 350
DTAAAIRAEL AKMEPFFPSG LKIVYPYDTT PFVKISIHEV VKTLVEAIIL
360 370 380 390 400
VFLVMYLFLQ NFRATLIPTI AVPVVLLGTF AVLAAFGFSI NTLTMFGMVL
410 420 430 440 450
AIGLLVDDAI VVVENVERVM AEEGLPPKEA TRKSMGQIQG ALVGIAMVLS
460 470 480 490 500
AVFVPMAFFG GSTGAIYRQF SITIVSAMAL SVLVALILTP ALCATMLKPI
510 520 530 540 550
AKGDHGEGKK GFFGWFNRMF EKSTHHYTDS VGGILRSTGR YLVLYLIIVV
560 570 580 590 600
GMAYLFVRLP SSFLPDEDQG VFMTMVQLPA GATQERTQKV LNEVTHYYLT
610 620 630 640 650
KEKNNVESVF AVNGFGFAGR GQNTGIAFVS LKDWADRPGE ENKVEAITMR
660 670 680 690 700
ATRAFSQIKD AMVFAFNLPA IVELGTATGF DFELIDQAGL GHEKLTQARN
710 720 730 740 750
QLLAEAAKHP DMLTSVRPNG LEDTPQFKID IDQEKAQALG VSINDINTTL
760 770 780 790 800
GAAWGGSYVN DFIDRGRVKK VYVMSEAKYR MLPDDIGDWY VRAADGQMVP
810 820 830 840 850
FSAFSSSRWE YGSPRLERYN GLPSMEILGQ AAPGKSTGEA MELMEQLASK
860 870 880 890 900
LPTGVGYDWT GMSYQERLSG NQAPSLYAIS LIVVFLCLAA LYESWSIPFS
910 920 930 940 950
VMLVVPLGVI GALLAATFRG LTNDVYFQVG LLTTIGLSAK NAILIVEFAK
960 970 980 990 1000
DLMDKEGKGL IEATLDAVRM RLRPILMTSL AFILGVMPLV ISTGAGSGAQ
1010 1020 1030 1040
NAVGTGVMGG MVTATVLAIF FVPVFFVVVR RRFSRKNEDI EHSHTVDHH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94248 Genomic DNA Translation: AAA23411.1 U00734 Genomic DNA Translation: AAA67135.1 U82664 Genomic DNA Translation: AAB40216.1 U00096 Genomic DNA Translation: AAC73564.1 AP009048 Genomic DNA Translation: BAE76241.1 |
PIRi | B36938 |
RefSeqi | NP_414995.1, NC_000913.3 WP_001132469.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73564; AAC73564; b0462 BAE76241; BAE76241; BAE76241 |
GeneIDi | 57731641 945108 |
KEGGi | ecj:JW0451 eco:b0462 |
PATRICi | fig|1411691.4.peg.1814 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94248 Genomic DNA Translation: AAA23411.1 U00734 Genomic DNA Translation: AAA67135.1 U82664 Genomic DNA Translation: AAB40216.1 U00096 Genomic DNA Translation: AAC73564.1 AP009048 Genomic DNA Translation: BAE76241.1 |
PIRi | B36938 |
RefSeqi | NP_414995.1, NC_000913.3 WP_001132469.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IWG | X-ray | 3.50 | A | 1-1049 | [»] | |
1OY6 | X-ray | 3.68 | A | 1-1049 | [»] | |
1OY8 | X-ray | 3.63 | A | 1-1049 | [»] | |
1OY9 | X-ray | 3.80 | A | 1-1049 | [»] | |
1OYD | X-ray | 3.80 | A | 1-1049 | [»] | |
1OYE | X-ray | 3.48 | A | 1-1049 | [»] | |
1T9T | X-ray | 3.23 | A | 1-1049 | [»] | |
1T9U | X-ray | 3.11 | A | 1-1049 | [»] | |
1T9V | X-ray | 3.80 | A | 1-1049 | [»] | |
1T9W | X-ray | 3.23 | A | 1-1049 | [»] | |
1T9X | X-ray | 3.08 | A | 1-1049 | [»] | |
1T9Y | X-ray | 3.64 | A | 1-1049 | [»] | |
2DHH | X-ray | 2.80 | A/B/C | 1-1049 | [»] | |
2DR6 | X-ray | 3.30 | A/B/C | 1-1049 | [»] | |
2DRD | X-ray | 3.10 | A/B/C | 1-1049 | [»] | |
2GIF | X-ray | 2.90 | A/B/C | 1-1049 | [»] | |
2HQC | X-ray | 3.56 | A | 1-1049 | [»] | |
2HQD | X-ray | 3.65 | A | 1-1049 | [»] | |
2HQF | X-ray | 3.38 | A | 1-1049 | [»] | |
2HQG | X-ray | 3.38 | A | 1-1049 | [»] | |
2HRT | X-ray | 3.00 | A/B/C/D/E/F | 1-1049 | [»] | |
2I6W | X-ray | 3.10 | A | 1-1049 | [»] | |
2J8S | X-ray | 2.54 | A/B/C | 1-1049 | [»] | |
2RDD | X-ray | 3.50 | A | 1-1049 | [»] | |
2W1B | X-ray | 3.85 | A | 1-1049 | [»] | |
3AOA | X-ray | 3.35 | A/B/C | 1-1049 | [»] | |
3AOB | X-ray | 3.35 | A/B/C | 1-1049 | [»] | |
3AOC | X-ray | 3.34 | A/B/C | 1-1049 | [»] | |
3AOD | X-ray | 3.30 | A/B/C | 1-1049 | [»] | |
3D9B | X-ray | 3.42 | A | 1-1049 | [»] | |
3NOC | X-ray | 2.70 | A/B/C | 1-1049 | [»] | |
3NOG | X-ray | 3.34 | A/B/C | 1-1049 | [»] | |
3W9H | X-ray | 3.05 | A/B/C | 1-1033 | [»] | |
4C48 | X-ray | 3.30 | A | 1-1047 | [»] | |
4CDI | X-ray | 3.70 | A | 1-1049 | [»] | |
4DX5 | X-ray | 1.90 | A/B/C | 1-1049 | [»] | |
4DX6 | X-ray | 2.90 | A/B/C | 1-1049 | [»] | |
4DX7 | X-ray | 2.25 | A/B/C | 1-1049 | [»] | |
4K7Q | X-ray | 3.50 | A | 1-1049 | [»] | |
4U8V | X-ray | 2.30 | A/B/C | 1-1049 | [»] | |
4U8Y | X-ray | 2.10 | A/B/C | 1-1049 | [»] | |
4U95 | X-ray | 2.00 | A/B/C | 1-1049 | [»] | |
4U96 | X-ray | 2.20 | A/B/C | 1-1049 | [»] | |
4ZIT | X-ray | 3.30 | A/B/C/D/E/F | 1-1049 | [»] | |
4ZIV | X-ray | 3.16 | A/B/C/D/E/F | 1-1049 | [»] | |
4ZIW | X-ray | 3.40 | A/B/C/D/E/F | 1-1049 | [»] | |
4ZJL | X-ray | 3.47 | A/B/C/D/E/F | 1-1049 | [»] | |
4ZJO | X-ray | 3.60 | A/B/C/D/E/F | 1-1049 | [»] | |
4ZJQ | X-ray | 3.59 | A/B/C/D/E/F | 1-1049 | [»] | |
4ZLJ | X-ray | 3.26 | A | 1-1049 | [»] | |
4ZLL | X-ray | 3.36 | A | 1-1049 | [»] | |
4ZLN | X-ray | 3.56 | A | 1-1049 | [»] | |
5EN5 | X-ray | 2.30 | A/B/C | 39-329 | [»] | |
A/B/C | 561-869 | [»] | ||||
5ENO | X-ray | 2.20 | A/B/C | 39-329 | [»] | |
A/B/C | 561-869 | [»] | ||||
5ENP | X-ray | 1.90 | A/B/C | 39-329 | [»] | |
A/B/C | 561-869 | [»] | ||||
5ENQ | X-ray | 1.80 | A/B/C | 39-329 | [»] | |
A/B/C | 561-869 | [»] | ||||
5ENR | X-ray | 2.30 | A/B/C | 39-329 | [»] | |
A/B/C | 561-869 | [»] | ||||
5ENS | X-ray | 2.80 | A/B/C | 39-329 | [»] | |
A/B/C | 561-869 | [»] | ||||
5ENT | X-ray | 2.50 | A/B/C | 39-329 | [»] | |
A/B/C | 561-869 | [»] | ||||
5JMN | X-ray | 2.50 | A/B/C | 1-1049 | [»] | |
5NC5 | X-ray | 3.20 | A/B/C | 1-1049 | [»] | |
5NG5 | electron microscopy | 6.50 | J/K/L | 1-1049 | [»] | |
5O66 | electron microscopy | 5.90 | J/K/L | 1-1049 | [»] | |
5V5S | electron microscopy | 6.50 | J/K/L | 1-1049 | [»] | |
5YIL | X-ray | 3.00 | A/B/C | 1-1049 | [»] | |
6BAJ | electron microscopy | 3.20 | A/B/C | 1-1049 | [»] | |
6CSX | electron microscopy | 3.00 | A/B/C | 1-1049 | [»] | |
6Q4N | X-ray | 2.80 | A/B/C | 1-1049 | [»] | |
6Q4O | X-ray | 2.80 | A/B/C | 1-1049 | [»] | |
6Q4P | X-ray | 2.80 | A/B/C | 1-1049 | [»] | |
6SGR | electron microscopy | 3.17 | A/B/C | 1-1049 | [»] | |
6SGS | electron microscopy | 3.20 | A/B/C | 1-1049 | [»] | |
6SGT | electron microscopy | 3.46 | A/B/C | 1-1049 | [»] | |
6SGU | electron microscopy | 3.27 | A/B/C | 1-1049 | [»] | |
PCDDBi | P31224 | |||||
SMRi | P31224 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259859, 386 interactors |
ComplexPortali | CPX-4263, AcrAB-TolC multidrug efflux transport complex |
DIPi | DIP-9049N |
IntActi | P31224, 9 interactors |
MINTi | P31224 |
STRINGi | 511145.b0462 |
Chemistry databases
ChEMBLi | CHEMBL1681614 |
DrugBanki | DB03619, Deoxycholic acid DB04209, Dequalinium DB03825, Rhodamine 6G |
Protein family/group databases
TCDBi | 2.A.6.2.2, the resistance-nodulation-cell division (rnd) superfamily |
Proteomic databases
jPOSTi | P31224 |
PaxDbi | P31224 |
PRIDEi | P31224 |
Genome annotation databases
EnsemblBacteriai | AAC73564; AAC73564; b0462 BAE76241; BAE76241; BAE76241 |
GeneIDi | 57731641 945108 |
KEGGi | ecj:JW0451 eco:b0462 |
PATRICi | fig|1411691.4.peg.1814 |
Organism-specific databases
EchoBASEi | EB1655 |
Phylogenomic databases
eggNOGi | COG0841, Bacteria |
HOGENOMi | CLU_002755_0_0_6 |
InParanoidi | P31224 |
PhylomeDBi | P31224 |
Enzyme and pathway databases
BioCyci | EcoCyc:ACRB-MONOMER MetaCyc:ACRB-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P31224 |
PROi | PR:P31224 |
Family and domain databases
Gene3Di | 3.30.2090.10, 2 hits |
InterProi | View protein in InterPro IPR027463, AcrB_DN_DC_subdom IPR001036, Acrflvin-R IPR004764, HAE1 |
PANTHERi | PTHR32063, PTHR32063, 1 hit |
Pfami | View protein in Pfam PF00873, ACR_tran, 1 hit |
PRINTSi | PR00702, ACRIFLAVINRP |
SUPFAMi | SSF82714, SSF82714, 2 hits |
TIGRFAMsi | TIGR00915, 2A0602, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACRB_ECOLI | |
Accessioni | P31224Primary (citable) accession number: P31224 Secondary accession number(s): Q2MBW5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 190 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families