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Protein

Phosphoglucosamine mutase

Gene

glmM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate. Can also catalyze the formation of glucose-6-P from glucose-1-P, although at a 1400-fold lower rate.1 Publication

Miscellaneous

Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism, with alpha-D-glucosamine 1,6-bisphosphate as an intermediate.

Catalytic activityi

Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Activity regulationi

Autophosphorylation is inhibited by inorganic phosphate or EDTA.1 Publication

Kineticsi

  1. KM=0.05 mM for glucosamine-6-P2 Publications
  2. KM=0.08 mM for glucosamine-1,6-diP2 Publications
  3. KM=0.5 mM for glucose-1,6-diP2 Publications
  4. KM=0.65 mM for glucose-1-P2 Publications
  5. KM=0.06 mM for ATP2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei102Phosphoserine intermediate1 Publication1
    Metal bindingi102Magnesium; via phosphate groupBy similarity1
    Metal bindingi241MagnesiumBy similarity1
    Metal bindingi243MagnesiumBy similarity1
    Metal bindingi245MagnesiumBy similarity1

    GO - Molecular functioni

    • magnesium ion binding Source: UniProtKB-UniRule
    • phosphoglucosamine mutase activity Source: EcoCyc
    • phosphomannomutase activity Source: GO_Central

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:PHOSGLUCOSAMINEMUT-MONOMER
    MetaCyc:PHOSGLUCOSAMINEMUT-MONOMER
    SABIO-RKiP31120

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoglucosamine mutase (EC:5.4.2.10)
    Gene namesi
    Name:glmM
    Synonyms:mrsA, yhbF
    Ordered Locus Names:b3176, JW3143
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11553 glmM

    Subcellular locationi

    GO - Cellular componenti

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi100S → A: 2% of wild-type activity. 1 Publication1
    Mutagenesisi100S → T: 20-fold increase in the non-specific phosphoglucomutase activity towards glucose-phosphate substrates (non aminated). 1 Publication1
    Mutagenesisi102S → A: Loss of activity in the absence or presence of glucosamine-1,6-diP. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00001478872 – 445Phosphoglucosamine mutaseAdd BLAST444

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei102Phosphoserine; by autocatalysis2 Publications1

    Post-translational modificationi

    Activated by phosphorylation. Can autophosphorylate in vitro using ATP, alpha-D-glucosamine 1,6-bisphosphate or alpha-D-glucose 1,6-bisphosphate.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    EPDiP31120
    PaxDbiP31120
    PRIDEiP31120

    PTM databases

    iPTMnetiP31120

    Interactioni

    Protein-protein interaction databases

    BioGridi4263256, 227 interactors
    DIPiDIP-10260N
    IntActiP31120, 16 interactors
    STRINGi316385.ECDH10B_3350

    Structurei

    3D structure databases

    ProteinModelPortaliP31120
    SMRiP31120
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phosphohexose mutase family.Curated

    Phylogenomic databases

    eggNOGiENOG4107QJF Bacteria
    COG1109 LUCA
    HOGENOMiHOG000268678
    InParanoidiP31120
    KOiK03431
    PhylomeDBiP31120

    Family and domain databases

    CDDicd05802 GlmM, 1 hit
    HAMAPiMF_01554_B GlmM_B, 1 hit
    InterProiView protein in InterPro
    IPR005844 A-D-PHexomutase_a/b/a-I
    IPR016055 A-D-PHexomutase_a/b/a-I/II/III
    IPR005845 A-D-PHexomutase_a/b/a-II
    IPR005846 A-D-PHexomutase_a/b/a-III
    IPR005843 A-D-PHexomutase_C
    IPR036900 A-D-PHexomutase_C_sf
    IPR016066 A-D-PHexomutase_CS
    IPR005841 Alpha-D-phosphohexomutase_SF
    IPR006352 GlmM_bact
    PfamiView protein in Pfam
    PF02878 PGM_PMM_I, 1 hit
    PF02879 PGM_PMM_II, 1 hit
    PF02880 PGM_PMM_III, 1 hit
    PF00408 PGM_PMM_IV, 1 hit
    PRINTSiPR00509 PGMPMM
    SUPFAMiSSF53738 SSF53738, 3 hits
    SSF55957 SSF55957, 1 hit
    TIGRFAMsiTIGR01455 glmM, 1 hit
    PROSITEiView protein in PROSITE
    PS00710 PGM_PMM, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31120-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSNRKYFGTD GIRGRVGDAP ITPDFVLKLG WAAGKVLARH GSRKIIIGKD
    60 70 80 90 100
    TRISGYMLES ALEAGLAAAG LSALFTGPMP TPAVAYLTRT FRAEAGIVIS
    110 120 130 140 150
    ASHNPFYDNG IKFFSIDGTK LPDAVEEAIE AEMEKEISCV DSAELGKASR
    160 170 180 190 200
    IVDAAGRYIE FCKATFPNEL SLSELKIVVD CANGATYHIA PNVLRELGAN
    210 220 230 240 250
    VIAIGCEPNG VNINAEVGAT DVRALQARVL AEKADLGIAF DGDGDRVIMV
    260 270 280 290 300
    DHEGNKVDGD QIMYIIAREG LRQGQLRGGA VGTLMSNMGL ELALKQLGIP
    310 320 330 340 350
    FARAKVGDRY VLEKMQEKGW RIGAENSGHV ILLDKTTTGD GIVAGLQVLA
    360 370 380 390 400
    AMARNHMSLH DLCSGMKMFP QILVNVRYTA GSGDPLEHES VKAVTAEVEA
    410 420 430 440
    ALGNRGRVLL RKSGTEPLIR VMVEGEDEAQ VTEFAHRIAD AVKAV
    Length:445
    Mass (Da):47,544
    Last modified:January 23, 2007 - v3
    Checksum:iECDF9A0378A980EC
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti69A → R in AAA97510 (Ref. 2) Curated1
    Sequence conflicti162C → S in AAA97510 (Ref. 2) Curated1
    Sequence conflicti167P → R in AAA97510 (Ref. 2) Curated1
    Sequence conflicti178 – 181VVDC → LVIG in AAA97510 (Ref. 2) Curated4
    Sequence conflicti411R → C in AAA97510 (Ref. 2) Curated1
    Sequence conflicti417P → R in AAA97510 (Ref. 2) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L12968 Unassigned DNA Translation: AAA16122.1
    U01376 Genomic DNA Translation: AAA97510.1
    U18997 Genomic DNA Translation: AAA57977.1
    U00096 Genomic DNA Translation: AAC76208.1
    AP009048 Genomic DNA Translation: BAE77220.1
    PIRiI41215
    RefSeqiNP_417643.1, NC_000913.3
    WP_000071134.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76208; AAC76208; b3176
    BAE77220; BAE77220; BAE77220
    GeneIDi947692
    KEGGiecj:JW3143
    eco:b3176
    PATRICifig|1411691.4.peg.3556

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L12968 Unassigned DNA Translation: AAA16122.1
    U01376 Genomic DNA Translation: AAA97510.1
    U18997 Genomic DNA Translation: AAA57977.1
    U00096 Genomic DNA Translation: AAC76208.1
    AP009048 Genomic DNA Translation: BAE77220.1
    PIRiI41215
    RefSeqiNP_417643.1, NC_000913.3
    WP_000071134.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP31120
    SMRiP31120
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4263256, 227 interactors
    DIPiDIP-10260N
    IntActiP31120, 16 interactors
    STRINGi316385.ECDH10B_3350

    PTM databases

    iPTMnetiP31120

    Proteomic databases

    EPDiP31120
    PaxDbiP31120
    PRIDEiP31120

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76208; AAC76208; b3176
    BAE77220; BAE77220; BAE77220
    GeneIDi947692
    KEGGiecj:JW3143
    eco:b3176
    PATRICifig|1411691.4.peg.3556

    Organism-specific databases

    EchoBASEiEB1514
    EcoGeneiEG11553 glmM

    Phylogenomic databases

    eggNOGiENOG4107QJF Bacteria
    COG1109 LUCA
    HOGENOMiHOG000268678
    InParanoidiP31120
    KOiK03431
    PhylomeDBiP31120

    Enzyme and pathway databases

    BioCyciEcoCyc:PHOSGLUCOSAMINEMUT-MONOMER
    MetaCyc:PHOSGLUCOSAMINEMUT-MONOMER
    SABIO-RKiP31120

    Miscellaneous databases

    PROiPR:P31120

    Family and domain databases

    CDDicd05802 GlmM, 1 hit
    HAMAPiMF_01554_B GlmM_B, 1 hit
    InterProiView protein in InterPro
    IPR005844 A-D-PHexomutase_a/b/a-I
    IPR016055 A-D-PHexomutase_a/b/a-I/II/III
    IPR005845 A-D-PHexomutase_a/b/a-II
    IPR005846 A-D-PHexomutase_a/b/a-III
    IPR005843 A-D-PHexomutase_C
    IPR036900 A-D-PHexomutase_C_sf
    IPR016066 A-D-PHexomutase_CS
    IPR005841 Alpha-D-phosphohexomutase_SF
    IPR006352 GlmM_bact
    PfamiView protein in Pfam
    PF02878 PGM_PMM_I, 1 hit
    PF02879 PGM_PMM_II, 1 hit
    PF02880 PGM_PMM_III, 1 hit
    PF00408 PGM_PMM_IV, 1 hit
    PRINTSiPR00509 PGMPMM
    SUPFAMiSSF53738 SSF53738, 3 hits
    SSF55957 SSF55957, 1 hit
    TIGRFAMsiTIGR01455 glmM, 1 hit
    PROSITEiView protein in PROSITE
    PS00710 PGM_PMM, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLMM_ECOLI
    AccessioniPrimary (citable) accession number: P31120
    Secondary accession number(s): Q2M936
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: January 23, 2007
    Last modified: November 7, 2018
    This is version 152 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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