UniProtKB - P31085 (PSAC_SYNP6)
Protein
Photosystem I iron-sulfur center
Gene
psaC
Organism
Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans)
Status
Functioni
Apoprotein for the two 4Fe-4S centers FA and FB of photosystem I (PSI); essential for photochemical activity. FB is the terminal electron acceptor of PSI, donating electrons to ferredoxin. The C-terminus interacts with PsaA/B/D and helps assemble the protein into the PSI complex. Required for binding of PsaD and PsaE to PSI. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn.1 Publication
Catalytic activityi
- hν + oxidized [2Fe-2S]-[ferredoxin] + reduced [plastocyanin] = oxidized [plastocyanin] + reduced [2Fe-2S]-[ferredoxin]EC:1.97.1.12
Cofactori
[4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters. Cluster 2 is most probably the spectroscopically characterized electron acceptor FA and cluster 1 is most probably FB.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 11 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 14 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 17 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 21 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 48 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 51 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 54 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 58 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- electron transfer activity Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- photosynthetic electron transport in photosystem I Source: InterPro
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Photosynthesis, Transport |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | SELO269084:G1G2F-1070-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Photosystem I iron-sulfur center (EC:1.97.1.12)Alternative name(s): 9 kDa polypeptide PSI-C Photosystem I subunit VII PsaC |
Gene namesi | Name:psaC Ordered Locus Names:syc0986_d |
Organismi | Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) (Anacystis nidulans) |
Taxonomic identifieri | 269084 [NCBI] |
Taxonomic lineagei | Bacteria › Cyanobacteria › Synechococcales › Synechococcaceae › Synechococcus › |
Proteomesi |
|
Subcellular locationi
Other locations
Plasma Membrane
- plasma membrane-derived thylakoid membrane Source: UniProtKB-SubCell
Other locations
- photosystem I Source: UniProtKB-KW
Keywords - Cellular componenti
Membrane, Photosystem I, ThylakoidPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | D → A: Decreases assembly onto PSI cores (in vitro). 1 Publication | 1 | |
Mutagenesisi | 9 | D → R: Decreases assembly onto PSI cores in vitro, FA and FB seems to assemble normally. 1 Publication | 1 | |
Mutagenesisi | 27 | E → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 27 | E → R: Decreases assembly onto PSI cores in vitro, FA and FB seem to assemble normally. 1 Publication | 1 | |
Mutagenesisi | 32 | D → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 32 | D → R: Decreases assembly onto PSI cores in vitro, FA and FB seems to assemble normally. 1 Publication | 1 | |
Mutagenesisi | 72 – 81 | Missing : Required for stable assembly of the protein onto PSI cores (in vitro). 1 Publication | 10 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000062022 | 2 – 81 | Photosystem I iron-sulfur centerAdd BLAST | 80 |
Interactioni
Subunit structurei
The cyanobacterial PSI reaction center is composed of one copy each of PsaA,B,C,D,E,F,I,J,K,L,M and X, and forms trimeric complexes.
Protein-protein interaction databases
STRINGi | 269084.syc0986_d |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 31 | 4Fe-4S ferredoxin-type 1Add BLAST | 30 | |
Domaini | 39 – 68 | 4Fe-4S ferredoxin-type 2Add BLAST | 30 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG1143, Bacteria |
OMAi | GHMSHAV |
Family and domain databases
HAMAPi | MF_01303, PSI_PsaC, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR017491, PSI_PsaC |
Pfami | View protein in Pfam PF12838, Fer4_7, 1 hit |
TIGRFAMsi | TIGR03048, PS_I_psaC, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P31085-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSHSVKIYDT CIGCTQCVRA CPLDVLEMVP WDGCKAGQIA ASPRTEDCVG
60 70 80
CKRCETACPT DFLSIRVYLG AETTRSMGLA Y
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U01536 Unassigned DNA Translation: AAA18220.1 AP008231 Genomic DNA Translation: BAD79176.1 |
RefSeqi | WP_011243298.1, NC_006576.1 |
Genome annotation databases
EnsemblBacteriai | BAD79176; BAD79176; syc0986_d |
KEGGi | syc:syc0986_d |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U01536 Unassigned DNA Translation: AAA18220.1 AP008231 Genomic DNA Translation: BAD79176.1 |
RefSeqi | WP_011243298.1, NC_006576.1 |
3D structure databases
SMRi | P31085 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 269084.syc0986_d |
Genome annotation databases
EnsemblBacteriai | BAD79176; BAD79176; syc0986_d |
KEGGi | syc:syc0986_d |
Phylogenomic databases
eggNOGi | COG1143, Bacteria |
OMAi | GHMSHAV |
Enzyme and pathway databases
BioCyci | SELO269084:G1G2F-1070-MONOMER |
Family and domain databases
HAMAPi | MF_01303, PSI_PsaC, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR017491, PSI_PsaC |
Pfami | View protein in Pfam PF12838, Fer4_7, 1 hit |
TIGRFAMsi | TIGR03048, PS_I_psaC, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSAC_SYNP6 | |
Accessioni | P31085Primary (citable) accession number: P31085 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 135 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |