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Protein

Dihydrofolate reductase

Gene

folA

Organism
Klebsiella aerogenes (Enterobacter aerogenes)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (folA), Dihydrofolate reductase (dfrA12), Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei5Substrate; via carbonyl oxygenBy similarity1
Binding sitei7NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei27SubstrateBy similarity1
Binding sitei52SubstrateBy similarity1
Binding sitei57SubstrateBy similarity1
Binding sitei76NADP; via carbonyl oxygenBy similarity1
Binding sitei113SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 19NADPBy similarity7
Nucleotide bindingi45 – 46NADPBy similarity2
Nucleotide bindingi63 – 64NADPBy similarity2
Nucleotide bindingi95 – 102NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:atsR
OrganismiKlebsiella aerogenes (Enterobacter aerogenes)
Taxonomic identifieri548 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3853

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863891 – 159Dihydrofolate reductaseAdd BLAST159

Structurei

3D structure databases

ProteinModelPortaliP31074
SMRiP31074
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 158DHFRPROSITE-ProRule annotationAdd BLAST158

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiENOG4108YYV Bacteria
COG0262 LUCA

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 2 hits
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PIRSFiPIRSF000194 DHFR, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P31074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISLIAALAV DRVIGMENAM PWDLPADLAW FKRNTLNKPV VMGRLTWESI
60 70 80 90 100
GRPLPGRKNI VISSKPGSDD RVQWVKSVDE AIAACGDAEE IMVIGGGRVY
110 120 130 140 150
EQFLPKAHKL YLTHIDAEVE GDTHFPDYDP DEWESVFSEF HDADAQNSHS

YCFEILERR
Length:159
Mass (Da):17,974
Last modified:July 1, 1993 - v1
Checksum:i73C9B8D31D0C1C07
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9A → R in BAA01187 (PubMed:1551851).Curated1
Sequence conflicti23 – 26DLPA → NLNE in BAA01187 (PubMed:1551851).Curated4
Sequence conflicti76K → S in BAA01187 (PubMed:1551851).Curated1
Sequence conflicti79D → E in BAA01187 (PubMed:1551851).Curated1
Sequence conflicti88A → V in BAA01187 (PubMed:1551851).Curated1
Sequence conflicti101 – 102EQ → DE in BAA01187 (PubMed:1551851).Curated2
Sequence conflicti108H → Q in BAA01187 (PubMed:1551851).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26022 Genomic DNA Translation: AAA24800.1
D10358 Genomic DNA Translation: BAA01187.1
PIRiA39799
RefSeqiWP_015368294.1, NZ_PQLE01000009.1

Similar proteinsi

Entry informationi

Entry nameiDYR_KLEAE
AccessioniPrimary (citable) accession number: P31074
Secondary accession number(s): P27498
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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