UniProtKB - P31060 (MODF_ECOLI)
Protein
ABC transporter ATP-binding protein ModF
Gene
modF
Organism
Escherichia coli (strain K12)
Status
Functioni
Probably not involved in the transport of molybdenum into the cell.1 Publication
Caution
Was originally thought to be PhrA, involved in photoreactivation. The protein was thought to be the C-terminus of what is now accepted to be a longer reading frame called ModF; overexpression from a plasmid yields an approximately 50 kDa protein, which is too long to be PhrA (PubMed:8564363).2 Publications1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 43 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 293 – 300 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: EcoCyc
GO - Biological processi
- DNA repair Source: UniProtKB-KW
Keywordsi
Biological process | DNA damage, DNA repair, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:MODF-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: ABC transporter ATP-binding protein ModFAlternative name(s): Photorepair protein PhrA1 Publication |
Gene namesi | Name:modF Synonyms:ORF61 Publication, phrA1 Publication Ordered Locus Names:b0760, JW0743 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane By similarity; Peripheral membrane protein By similarity
Plasma Membrane
- ATP-binding cassette (ABC) transporter complex Source: GO_Central
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Not required for synthesis of the molybdenum cofactor or for the uptake of molybdate (PubMed:8564363).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092566 | 1 – 490 | ABC transporter ATP-binding protein ModFAdd BLAST | 490 |
Proteomic databases
jPOSTi | P31060 |
PaxDbi | P31060 |
PRIDEi | P31060 |
Expressioni
Inductioni
Probably part of the modE-modF operon.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263124, 95 interactors 849744, 1 interactor |
IntActi | P31060, 7 interactors |
STRINGi | 511145.b0760 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 235 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 232 | |
Domaini | 261 – 489 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 229 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG1119, Bacteria |
HOGENOMi | CLU_000604_45_0_6 |
InParanoidi | P31060 |
PhylomeDBi | P31060 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS50893, ABC_TRANSPORTER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
P31060-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSLQILQGT FRLSDTKTLQ LPQLTLNAGD SWAFVGSNGS GKSALARALA
60 70 80 90 100
GELPLLKGER QSQFSHITRL SFEQLQKLVS DEWQRNNTDM LGPGEDDTGR
110 120 130 140 150
TTAEIIQDEV KDAPRCMQLA QQFGITALLD RRFKYLSTGE TRKTLLCQAL
160 170 180 190 200
MSEPDLLILD EPFDGLDVAS RQQLAERLAS LHQSGITLVL VLNRFDEIPE
210 220 230 240 250
FVQFAGVLAD CTLAETGAKE ELLQQALVAQ LAHSEQLEGV QLPEPDEPSA
260 270 280 290 300
RHALPANEPR IVLNNGVVSY NDRPILNNLS WQVNPGEHWQ IVGPNGAGKS
310 320 330 340 350
TLLSLVTGDH PQGYSNDLTL FGRRRGSGET IWDIKKHIGY VSSSLHLDYR
360 370 380 390 400
VSTTVRNVIL SGYFDSIGIY QAVSDRQQKL VQQWLDILGI DKRTADAPFH
410 420 430 440 450
SLSWGQQRLA LIVRALVKHP TLLILDEPLQ GLDPLNRQLI RRFVDVLISE
460 470 480 490
GETQLLFVSH HAEDAPACIT HRLEFVPDGG LYRYVLTKIY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 320 | L → A in CAA48926 (PubMed:8310005).Curated | 1 | |
Sequence conflicti | 322 | G → R in CAA48926 (PubMed:8310005).Curated | 1 | |
Sequence conflicti | 325 | R → A in CAA48926 (PubMed:8310005).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07867 Genomic DNA Translation: AAB06891.1 U27192 Genomic DNA Translation: AAB60176.1 U00096 Genomic DNA Translation: AAC73847.1 AP009048 Genomic DNA Translation: BAA35422.1 X69182 Genomic DNA Translation: CAA48926.1 |
PIRi | JC6038 |
RefSeqi | NP_415281.1, NC_000913.3 WP_000096869.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73847; AAC73847; b0760 BAA35422; BAA35422; BAA35422 |
GeneIDi | 945368 |
KEGGi | ecj:JW0743 eco:b0760 |
PATRICi | fig|1411691.4.peg.1518 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07867 Genomic DNA Translation: AAB06891.1 U27192 Genomic DNA Translation: AAB60176.1 U00096 Genomic DNA Translation: AAC73847.1 AP009048 Genomic DNA Translation: BAA35422.1 X69182 Genomic DNA Translation: CAA48926.1 |
PIRi | JC6038 |
RefSeqi | NP_415281.1, NC_000913.3 WP_000096869.1, NZ_SSZK01000002.1 |
3D structure databases
SMRi | P31060 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263124, 95 interactors 849744, 1 interactor |
IntActi | P31060, 7 interactors |
STRINGi | 511145.b0760 |
Proteomic databases
jPOSTi | P31060 |
PaxDbi | P31060 |
PRIDEi | P31060 |
Genome annotation databases
EnsemblBacteriai | AAC73847; AAC73847; b0760 BAA35422; BAA35422; BAA35422 |
GeneIDi | 945368 |
KEGGi | ecj:JW0743 eco:b0760 |
PATRICi | fig|1411691.4.peg.1518 |
Organism-specific databases
EchoBASEi | EB1628 |
Phylogenomic databases
eggNOGi | COG1119, Bacteria |
HOGENOMi | CLU_000604_45_0_6 |
InParanoidi | P31060 |
PhylomeDBi | P31060 |
Enzyme and pathway databases
BioCyci | EcoCyc:MODF-MONOMER |
Miscellaneous databases
PROi | PR:P31060 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS50893, ABC_TRANSPORTER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MODF_ECOLI | |
Accessioni | P31060Primary (citable) accession number: P31060 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families