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Protein

Phosphatidylethanolamine-binding protein 1

Gene

Pebp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation (By similarity).By similarity
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.

Miscellaneous

Seems to be associated with memory and learning disorder.

GO - Molecular functioni

  • ATP binding Source: RGD
  • kinase binding Source: RGD
  • lipid binding Source: RGD
  • mitogen-activated protein kinase binding Source: RGD
  • protein kinase binding Source: RGD
  • receptor serine/threonine kinase binding Source: RGD
  • serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
  • signaling receptor binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor
LigandATP-binding, Lipid-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-5674135 MAP2K and MAPK activation
R-RNO-5675221 Negative regulation of MAPK pathway

Protein family/group databases

MEROPSiI51.002

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylethanolamine-binding protein 1
Short name:
PEBP-1
Alternative name(s):
23 kDa morphine-binding protein
HCNPpp
P23K
Cleaved into the following chain:
Gene namesi
Name:Pebp1
Synonyms:Pbp, Pebp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi62017 Pebp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000232772 – 187Phosphatidylethanolamine-binding protein 1Add BLAST186
PeptideiPRO_00000232782 – 12Hippocampal cholinergic neurostimulating peptideBy similarityAdd BLAST11

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine; in peptide hippocampal cholinergic neurostimulating1 Publication1
Modified residuei6PhosphoserineBy similarity1
Modified residuei13PhosphoserineCombined sources1
Modified residuei42PhosphothreonineBy similarity1
Modified residuei52PhosphoserineCombined sources1
Modified residuei54PhosphoserineCombined sources1
Modified residuei98PhosphoserineBy similarity1
Modified residuei153PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP31044
PRIDEiP31044

2D gel databases

World-2DPAGEi0004:P31044

PTM databases

iPTMnetiP31044
PhosphoSitePlusiP31044

Expressioni

Tissue specificityi

Major component of epididymal secretions and sperm plasma membranes. It is present in cytosols from a variety of other tissues. Highly expressed in brain.

Gene expression databases

BgeeiENSRNOG00000001136 Expressed in 10 organ(s), highest expression level in testis
GenevisibleiP31044 RN

Interactioni

Subunit structurei

Has a tendency to form dimers by disulfide cross-linking. Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Interacts with ALOX15; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248176, 11 interactors
STRINGi10116.ENSRNOP00000001500

Structurei

Secondary structure

1187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP31044
SMRiP31044
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31044

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 134Interaction with RAF1By similarityAdd BLAST42

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3346 Eukaryota
COG1881 LUCA
GeneTreeiENSGT00900000141013
HOGENOMiHOG000237655
HOVERGENiHBG008165
InParanoidiP31044
OMAiCFQAEWD
OrthoDBiEOG091G0KH6
PhylomeDBiP31044
TreeFamiTF315074

Family and domain databases

CDDicd00866 PEBP_euk, 1 hit
Gene3Di3.90.280.10, 1 hit
InterProiView protein in InterPro
IPR008914 PEBP
IPR036610 PEBP-like_sf
IPR035810 PEBP_euk
IPR001858 Phosphatidylethanolamine-bd_CS
PANTHERiPTHR11362 PTHR11362, 1 hit
PfamiView protein in Pfam
PF01161 PBP, 1 hit
SUPFAMiSSF49777 SSF49777, 1 hit
PROSITEiView protein in PROSITE
PS01220 PBP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31044-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAADISQWAG PLSLQEVDEP PQHALRVDYG GVTVDELGKV LTPTQVMNRP
60 70 80 90 100
SSISWDGLDP GKLYTLVLTD PDAPSRKDPK FREWHHFLVV NMKGNDISSG
110 120 130 140 150
TVLSEYVGSG PPKDTGLHRY VWLVYEQEQP LNCDEPILSN KSGDNRGKFK
160 170 180
VESFRKKYHL GAPVAGTCFQ AEWDDSVPKL HDQLAGK
Length:187
Mass (Da):20,801
Last modified:January 23, 2007 - v3
Checksum:iF2BF053FE34B8056
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49R → G AA sequence (PubMed:1932083).Curated1
Sequence conflicti54 – 55SW → TA AA sequence (PubMed:1932083).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75253 mRNA Translation: CAA53032.1
X75254 Genomic DNA Translation: CAA53033.1
X71873 mRNA Translation: CAA50708.1
BC063171 mRNA Translation: AAH63171.1
PIRiA36126
S18358
RefSeqiNP_058932.1, NM_017236.1
UniGeneiRn.29745

Genome annotation databases

EnsembliENSRNOT00000001500; ENSRNOP00000001500; ENSRNOG00000001136
GeneIDi29542
KEGGirno:29542
UCSCiRGD:62017 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75253 mRNA Translation: CAA53032.1
X75254 Genomic DNA Translation: CAA53033.1
X71873 mRNA Translation: CAA50708.1
BC063171 mRNA Translation: AAH63171.1
PIRiA36126
S18358
RefSeqiNP_058932.1, NM_017236.1
UniGeneiRn.29745

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IQXX-ray2.20A/B/C1-187[»]
2IQYX-ray1.40A1-187[»]
6ENTX-ray2.66A2-187[»]
ProteinModelPortaliP31044
SMRiP31044
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248176, 11 interactors
STRINGi10116.ENSRNOP00000001500

Protein family/group databases

MEROPSiI51.002

PTM databases

iPTMnetiP31044
PhosphoSitePlusiP31044

2D gel databases

World-2DPAGEi0004:P31044

Proteomic databases

PaxDbiP31044
PRIDEiP31044

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001500; ENSRNOP00000001500; ENSRNOG00000001136
GeneIDi29542
KEGGirno:29542
UCSCiRGD:62017 rat

Organism-specific databases

CTDi5037
RGDi62017 Pebp1

Phylogenomic databases

eggNOGiKOG3346 Eukaryota
COG1881 LUCA
GeneTreeiENSGT00900000141013
HOGENOMiHOG000237655
HOVERGENiHBG008165
InParanoidiP31044
OMAiCFQAEWD
OrthoDBiEOG091G0KH6
PhylomeDBiP31044
TreeFamiTF315074

Enzyme and pathway databases

ReactomeiR-RNO-5674135 MAP2K and MAPK activation
R-RNO-5675221 Negative regulation of MAPK pathway

Miscellaneous databases

EvolutionaryTraceiP31044
PROiPR:P31044

Gene expression databases

BgeeiENSRNOG00000001136 Expressed in 10 organ(s), highest expression level in testis
GenevisibleiP31044 RN

Family and domain databases

CDDicd00866 PEBP_euk, 1 hit
Gene3Di3.90.280.10, 1 hit
InterProiView protein in InterPro
IPR008914 PEBP
IPR036610 PEBP-like_sf
IPR035810 PEBP_euk
IPR001858 Phosphatidylethanolamine-bd_CS
PANTHERiPTHR11362 PTHR11362, 1 hit
PfamiView protein in Pfam
PF01161 PBP, 1 hit
SUPFAMiSSF49777 SSF49777, 1 hit
PROSITEiView protein in PROSITE
PS01220 PBP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPEBP1_RAT
AccessioniPrimary (citable) accession number: P31044
Secondary accession number(s): P31045
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 157 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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