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UniProtKB - P31041 (CD28_MOUSE)
Protein
T-cell-specific surface glycoprotein CD28
Gene
Cd28
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
- protease binding Source: MGI
- protein kinase binding Source: MGI
GO - Biological processi
- apoptotic signaling pathway Source: MGI
- immune response Source: InterPro
- negative regulation of gene expression Source: MGI
- negative thymic T cell selection Source: MGI
- positive regulation of alpha-beta T cell proliferation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of inflammatory response to antigenic stimulus Source: MGI
- positive regulation of interleukin-10 production Source: UniProtKB
- positive regulation of interleukin-2 production Source: MGI
- positive regulation of interleukin-4 production Source: UniProtKB
- positive regulation of isotype switching to IgG isotypes Source: MGI
- positive regulation of mitotic nuclear division Source: UniProtKB
- positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
- positive regulation of protein kinase B signaling Source: MGI
- positive regulation of T cell proliferation Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of regulatory T cell differentiation Source: MGI
- regulatory T cell differentiation Source: MGI
- T cell activation Source: MGI
- T cell costimulation Source: MGI
- T cell receptor signaling pathway Source: MGI
Enzyme and pathway databases
Reactomei | R-MMU-1257604, PIP3 activates AKT signaling R-MMU-389356, CD28 co-stimulation R-MMU-389357, CD28 dependent PI3K/Akt signaling R-MMU-389359, CD28 dependent Vav1 pathway R-MMU-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling |
Names & Taxonomyi
Protein namesi | Recommended name: T-cell-specific surface glycoprotein CD28Alternative name(s): CD_antigen: CD28 |
Gene namesi | Name:Cd28 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88327, Cd28 |
VEuPathDBi | HostDB:ENSMUSG00000026012 |
Subcellular locationi
Other locations
Plasma Membrane
- external side of plasma membrane Source: MGI
- immunological synapse Source: MGI
- plasma membrane Source: MGI
Other locations
- cell surface Source: MGI
- integral component of membrane Source: UniProtKB-KW
- membrane raft Source: MGI
- protein complex involved in cell adhesion Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 150 | ExtracellularSequence analysisAdd BLAST | 131 | |
Transmembranei | 151 – 177 | HelicalSequence analysisAdd BLAST | 27 | |
Topological domaini | 178 – 218 | CytoplasmicSequence analysisAdd BLAST | 41 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | By similarityAdd BLAST | 19 | |
ChainiPRO_0000014653 | 20 – 218 | T-cell-specific surface glycoprotein CD28Add BLAST | 199 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 41 ↔ 113 | By similarity | ||
Disulfide bondi | 67 ↔ 87 | By similarity | ||
Glycosylationi | 72 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 93 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 106 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 130 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 187 | PhosphoserineBy similarity | 1 | |
Modified residuei | 189 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 207 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
EPDi | P31041 |
PaxDbi | P31041 |
PeptideAtlasi | P31041 |
PRIDEi | P31041 |
ProteomicsDBi | 280017 |
PTM databases
GlyGeni | P31041, 4 sites |
iPTMneti | P31041 |
PhosphoSitePlusi | P31041 |
SwissPalmi | P31041 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026012, Expressed in thymus and 108 other tissues |
Genevisiblei | P31041, MM |
Interactioni
Subunit structurei
Homodimer; disulfide-linked.
Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70.
Interacts with GRB2 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
- protease binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
DIPi | DIP-60858N |
IntActi | P31041, 3 interactors |
STRINGi | 10090.ENSMUSP00000027165 |
Miscellaneous databases
RNActi | P31041, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P31041 |
SMRi | P31041 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 138 | Ig-like V-typeAdd BLAST | 110 |
Keywords - Domaini
Immunoglobulin domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502SAVP, Eukaryota |
GeneTreei | ENSGT00530000063873 |
HOGENOMi | CLU_085095_1_0_1 |
InParanoidi | P31041 |
OMAi | CNYTYNG |
OrthoDBi | 1222373at2759 |
PhylomeDBi | P31041 |
TreeFami | TF335679 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR008093, CD28 IPR040216, CTLA4/CD28 IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013106, Ig_V-set |
PANTHERi | PTHR11494, PTHR11494, 1 hit |
Pfami | View protein in Pfam PF15910, V-set_2, 1 hit |
PRINTSi | PR01717, CD28ANTIGEN |
SUPFAMi | SSF48726, SSF48726, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P31041-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK
60 70 80 90 100
EFRASLYKGV NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL
110 120 130 140 150
WNLHVNHTDI YFCKIEFMYP PPYLDNERSN GTIIHIKEKH LCHTQSSPKL
160 170 180 190 200
FWALVVVAGV LFCYGLLVTV ALCVIWTNSR RNRLLQSDYM NMTPRRPGLT
210
RKPYQPYAPA RDFAAYRP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 187 – 189 | SDY → VTT in AAA37395 (PubMed:2157764).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34563 mRNA Translation: AAA37395.1 AL646054 Genomic DNA No translation available. AL672024 Genomic DNA No translation available. CR936842 Genomic DNA Translation: CAM13249.1 CH466548 Genomic DNA Translation: EDL00156.1 BC064058 mRNA Translation: AAH64058.1 M74361 mRNA Translation: AAA37396.1 |
CCDSi | CCDS14992.1 |
PIRi | A43523 I49584 |
RefSeqi | NP_031668.3, NM_007642.4 |
Genome annotation databases
Ensembli | ENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012 |
GeneIDi | 12487 |
KEGGi | mmu:12487 |
UCSCi | uc007bev.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34563 mRNA Translation: AAA37395.1 AL646054 Genomic DNA No translation available. AL672024 Genomic DNA No translation available. CR936842 Genomic DNA Translation: CAM13249.1 CH466548 Genomic DNA Translation: EDL00156.1 BC064058 mRNA Translation: AAH64058.1 M74361 mRNA Translation: AAA37396.1 |
CCDSi | CCDS14992.1 |
PIRi | A43523 I49584 |
RefSeqi | NP_031668.3, NM_007642.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2NAE | NMR | - | A | 177-218 | [»] | |
AlphaFoldDBi | P31041 | |||||
SMRi | P31041 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-60858N |
IntActi | P31041, 3 interactors |
STRINGi | 10090.ENSMUSP00000027165 |
PTM databases
GlyGeni | P31041, 4 sites |
iPTMneti | P31041 |
PhosphoSitePlusi | P31041 |
SwissPalmi | P31041 |
Proteomic databases
EPDi | P31041 |
PaxDbi | P31041 |
PeptideAtlasi | P31041 |
PRIDEi | P31041 |
ProteomicsDBi | 280017 |
Protocols and materials databases
Antibodypediai | 19958, 2984 antibodies from 48 providers |
DNASUi | 12487 |
Genome annotation databases
Ensembli | ENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012 |
GeneIDi | 12487 |
KEGGi | mmu:12487 |
UCSCi | uc007bev.2, mouse |
Organism-specific databases
CTDi | 940 |
MGIi | MGI:88327, Cd28 |
VEuPathDBi | HostDB:ENSMUSG00000026012 |
Phylogenomic databases
eggNOGi | ENOG502SAVP, Eukaryota |
GeneTreei | ENSGT00530000063873 |
HOGENOMi | CLU_085095_1_0_1 |
InParanoidi | P31041 |
OMAi | CNYTYNG |
OrthoDBi | 1222373at2759 |
PhylomeDBi | P31041 |
TreeFami | TF335679 |
Enzyme and pathway databases
Reactomei | R-MMU-1257604, PIP3 activates AKT signaling R-MMU-389356, CD28 co-stimulation R-MMU-389357, CD28 dependent PI3K/Akt signaling R-MMU-389359, CD28 dependent Vav1 pathway R-MMU-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling |
Miscellaneous databases
BioGRID-ORCSi | 12487, 2 hits in 112 CRISPR screens |
PROi | PR:P31041 |
RNActi | P31041, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026012, Expressed in thymus and 108 other tissues |
Genevisiblei | P31041, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR008093, CD28 IPR040216, CTLA4/CD28 IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013106, Ig_V-set |
PANTHERi | PTHR11494, PTHR11494, 1 hit |
Pfami | View protein in Pfam PF15910, V-set_2, 1 hit |
PRINTSi | PR01717, CD28ANTIGEN |
SUPFAMi | SSF48726, SSF48726, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CD28_MOUSE | |
Accessioni | P31041Primary (citable) accession number: P31041 Secondary accession number(s): Q6GSH7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | October 3, 2012 | |
Last modified: | May 25, 2022 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references