Cd28

Functionⁱ

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation.By similarity

GO - Molecular functionⁱ

protease binding Source: MGI
protein homodimerization activity Source: MGI
protein kinase binding
Source: MGI
Inferred from physical interactionⁱ
- 21245484
SH3/SH2 adaptor activity Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 10429671
immune response Source: InterPro
negative regulation of gene expression Source: MGI
negative thymic T cell selection
Source: MGI
Inferred from mutant phenotypeⁱ
- 10429671
positive regulation of alpha-beta T cell proliferation
Source: MGI
Inferred from direct assayⁱ
positive regulation of gene expression Source: MGI
positive regulation of inflammatory response to antigenic stimulus
Source: MGI
Inferred from mutant phenotypeⁱ
- 10791997
positive regulation of interleukin-10 production Source: UniProtKB
positive regulation of interleukin-2 biosynthetic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 7688139
positive regulation of interleukin-4 production Source: UniProtKB
positive regulation of isotype switching to IgG isotypes
Source: MGI
Inferred from mutant phenotypeⁱ
- 7688139
positive regulation of mitotic nuclear division Source: UniProtKB
positive regulation of phosphatidylinositol 3-kinase signaling
Source: MGI
Inferred from direct assayⁱ
- 23589618
positive regulation of protein kinase B signaling
Source: MGI
Inferred from direct assayⁱ
- 23589618
positive regulation of T cell proliferation
Source: MGI
Inferred from direct assayⁱ
positive regulation of transcription by RNA polymerase II
Source: MGI
Inferred from genetic interactionⁱ
- 16809611
regulation of regulatory T cell differentiation
Source: MGI
Inferred from genetic interactionⁱ
- 21245484
regulatory T cell differentiation Source: MGI
T cell activation Source: MGI
T cell costimulation
Source: MGI
Inferred from direct assayⁱ
T cell receptor signaling pathway
Source: MGI
Inferred from mutant phenotypeⁱ
- 23793062

View the complete GO annotation on QuickGO ...

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1257604 PIP3 activates AKT signaling R-MMU-389356 CD28 co-stimulation R-MMU-389357 CD28 dependent PI3K/Akt signaling R-MMU-389359 CD28 dependent Vav1 pathway R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Reactomeⁱ

R-MMU-1257604 PIP3 activates AKT signaling
R-MMU-389356 CD28 co-stimulation
R-MMU-389357 CD28 dependent PI3K/Akt signaling
R-MMU-389359 CD28 dependent Vav1 pathway
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: T-cell-specific surface glycoprotein CD28 Alternative name(s): CD_antigen: CD28
Gene namesⁱ	Name:Cd28
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 1

Organism-specific databases

MGIⁱ	MGI:88327 Cd28

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	20 – 150	ExtracellularSequence analysisAdd BLAST	131
Transmembraneⁱ	151 – 177	HelicalSequence analysisAdd BLAST	27
Topological domainⁱ	178 – 218	CytoplasmicSequence analysisAdd BLAST	41

Keywords - Cellular componentⁱ

Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 19	By similarityAdd BLAST		19
ChainⁱPRO_0000014653	20 – 218	T-cell-specific surface glycoprotein CD28Add BLAST		199

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	41 ↔ 113	By similarity
Disulfide bondⁱ	67 ↔ 87	By similarity
Glycosylationⁱ	72	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	93	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	106	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	130	N-linked (GlcNAc...) asparagineSequence analysis	1
Modified residueⁱ	187	PhosphoserineBy similarity	1
Modified residueⁱ	189	PhosphotyrosineBy similarity	1
Modified residueⁱ	207	PhosphotyrosineBy similarity	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDⁱ	P31041
PaxDbⁱ	P31041
PeptideAtlas More...PeptideAtlasⁱ	P31041
PRIDEⁱ	P31041

PTM databases

iPTMnetⁱ	P31041
PhosphoSitePlusⁱ	P31041
SwissPalmⁱ	P31041

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000026012 Expressed in 74 organ(s), highest expression level in thymus
CleanExⁱ	MM_CD28
Genevisibleⁱ	P31041 MM

Interactionⁱ

Subunit structureⁱ

Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Interacts with GRB2 (By similarity).By similarity

GO - Molecular functionⁱ

protease binding Source: MGI
protein homodimerization activity Source: MGI
protein kinase binding
Source: MGI
Inferred from physical interactionⁱ
- 21245484
SH3/SH2 adaptor activity Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

Database of interacting proteins More...DIPⁱ	DIP-60858N
IntActⁱ	P31041, 3 interactors
STRINGⁱ	10090.ENSMUSP00000027165

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Beta strandⁱ	185 – 187	Combined sources		3
Turnⁱ	214 – 216	Combined sources		3

Show more details

3D structure databases

ProteinModelPortalⁱ	P31041
SMRⁱ	P31041
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	29 – 138	Ig-like V-typeAdd BLAST		110

Keywords - Domainⁱ

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IXMM Eukaryota ENOG410Z063 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063873
HOGENOMⁱ	HOG000276892
HOVERGENⁱ	HBG004094
InParanoidⁱ	P31041
KEGG Orthology (KO) More...KOⁱ	K06470
OMAⁱ	PTRRHYQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0IGY
TreeFamⁱ	TF335679

Family and domain databases

Gene3Dⁱ	2.60.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR008093 CD28 IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR013106 Ig_V-set
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF15910 V-set_2, 1 hit
PRINTSⁱ	PR01717 CD28ANTIGEN
SUPFAMⁱ	SSF48726 SSF48726, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P31041-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTLRLLFLAL NFFSVQVTEN KILVKQSPLL VVDSNEVSLS CRYSYNLLAK 
        60         70         80         90        100
EFRASLYKGV NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL 
       110        120        130        140        150
WNLHVNHTDI YFCKIEFMYP PPYLDNERSN GTIIHIKEKH LCHTQSSPKL 
       160        170        180        190        200
FWALVVVAGV LFCYGLLVTV ALCVIWTNSR RNRLLQSDYM NMTPRRPGLT 
       210 
RKPYQPYAPA RDFAAYRP

Length:218

Mass (Da):25,243

Last modified:October 3, 2012 - v2

Checksum:ⁱC8E1339DD7E53CA6

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	187 – 189	SDY → VTT in AAA37395 (PubMed:2157764).Curated		3

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M34563 mRNA Translation: AAA37395.1 AL646054 Genomic DNA Translation: CAI24197.1 AL672024 Genomic DNA Translation: CAI26227.1 CR936842 Genomic DNA Translation: CAM13249.1 CH466548 Genomic DNA Translation: EDL00156.1 BC064058 mRNA Translation: AAH64058.1 M74361 mRNA Translation: AAA37396.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS14992.1
PIRⁱ	A43523 I49584
NCBI Reference Sequences More...RefSeqⁱ	NP_031668.3, NM_007642.4
UniGeneⁱ	Mm.255003

Genome annotation databases

Ensemblⁱ	ENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012
GeneIDⁱ	12487
KEGGⁱ	mmu:12487
UCSC genome browser More...UCSCⁱ	uc007bev.2 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P31041	T-cell-specific surface glycoprotein CD28		RAT		218	UniRef90_P31041
Uncharacterized protein		MOUSE		218
UPI000A3119AB		MUSPA		219
UPI000A319FF9		MUSCR		219
CD28 antigen		RAT		218

Protein	Similar proteins		Species	Score	Length	Source
P31041	T-cell-specific surface glycoprotein CD28	)	HUMAN		220	UniRef50_P10747
T-cell-specific surface glycoprotein CD28		RAT		218
CD28 isoform 3		PANTR		220
Uncharacterized protein		PANPA		220
CD28 molecule		GORGO		220
+86

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M34563 mRNA Translation: AAA37395.1 AL646054 Genomic DNA Translation: CAI24197.1 AL672024 Genomic DNA Translation: CAI26227.1 CR936842 Genomic DNA Translation: CAM13249.1 CH466548 Genomic DNA Translation: EDL00156.1 BC064058 mRNA Translation: AAH64058.1 M74361 mRNA Translation: AAA37396.1
CCDSⁱ	CCDS14992.1
PIRⁱ	A43523 I49584
RefSeqⁱ	NP_031668.3, NM_007642.4
UniGeneⁱ	Mm.255003

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2NAE	NMR	-	A	177-218	[»]
ProteinModelPortalⁱ	P31041
SMRⁱ	P31041
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

DIPⁱ	DIP-60858N
IntActⁱ	P31041, 3 interactors
STRINGⁱ	10090.ENSMUSP00000027165

PTM databases

iPTMnetⁱ	P31041
PhosphoSitePlusⁱ	P31041
SwissPalmⁱ	P31041

Proteomic databases

EPDⁱ	P31041
PaxDbⁱ	P31041
PeptideAtlasⁱ	P31041
PRIDEⁱ	P31041

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000027165; ENSMUSP00000027165; ENSMUSG00000026012
GeneIDⁱ	12487
KEGGⁱ	mmu:12487
UCSCⁱ	uc007bev.2 mouse

Organism-specific databases

CTDⁱ	940
MGIⁱ	MGI:88327 Cd28

Phylogenomic databases

eggNOGⁱ	ENOG410IXMM Eukaryota ENOG410Z063 LUCA
GeneTreeⁱ	ENSGT00530000063873
HOGENOMⁱ	HOG000276892
HOVERGENⁱ	HBG004094
InParanoidⁱ	P31041
KOⁱ	K06470
OMAⁱ	PTRRHYQ
OrthoDBⁱ	EOG091G0IGY
TreeFamⁱ	TF335679

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1257604 PIP3 activates AKT signaling R-MMU-389356 CD28 co-stimulation R-MMU-389357 CD28 dependent PI3K/Akt signaling R-MMU-389359 CD28 dependent Vav1 pathway R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Reactomeⁱ

R-MMU-1257604 PIP3 activates AKT signaling
R-MMU-389356 CD28 co-stimulation
R-MMU-389357 CD28 dependent PI3K/Akt signaling
R-MMU-389359 CD28 dependent Vav1 pathway
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P31041
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000026012 Expressed in 74 organ(s), highest expression level in thymus
CleanExⁱ	MM_CD28
Genevisibleⁱ	P31041 MM

Family and domain databases

Gene3Dⁱ	2.60.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR008093 CD28 IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR013106 Ig_V-set
Pfamⁱ	View protein in Pfam PF15910 V-set_2, 1 hit
PRINTSⁱ	PR01717 CD28ANTIGEN
SUPFAMⁱ	SSF48726 SSF48726, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CD28_MOUSE
Accessionⁱ	P31041Primary (citable) accession number: P31041 Secondary accession number(s): Q6GSH7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
	Last sequence update:	October 3, 2012
	Last modified:	November 7, 2018
	This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

UniParc

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UniRef

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Supporting data

UniProtKB - P31041 (CD28_MOUSE)

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T-cell-specific surface glycoprotein CD28

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Plasma Membrane

Other locations

Topology

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ