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Protein

5-hydroxytryptamine receptor 2B

Gene

Htr2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:1505525, PubMed:1331748). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances (PubMed:22525520). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors (PubMed:1505525, PubMed:1331748). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores (By similarity). Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity). May play a role in the perception of pain (PubMed:22525520).By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei208Hydrophobic barrier that decreases the speed of ligand binding and dissociationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBehavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 2B
Short name:
5-HT-2B
Short name:
5-HT2B
Alternative name(s):
5-HT-2F1 Publication
Serotonin receptor 2B
Stomach fundus serotonin receptor1 Publication
Gene namesi
Name:Htr2b
Synonyms:Srl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61801 Htr2b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55ExtracellularBy similarityAdd BLAST55
Transmembranei56 – 78Helical; Name=1By similarityAdd BLAST23
Topological domaini79 – 89CytoplasmicBy similarityAdd BLAST11
Transmembranei90 – 112Helical; Name=2By similarityAdd BLAST23
Topological domaini113 – 128ExtracellularBy similarityAdd BLAST16
Transmembranei129 – 150Helical; Name=3By similarityAdd BLAST22
Topological domaini151 – 170CytoplasmicBy similarityAdd BLAST20
Transmembranei171 – 191Helical; Name=4By similarityAdd BLAST21
Topological domaini192 – 215ExtracellularBy similarityAdd BLAST24
Transmembranei216 – 238Helical; Name=5By similarityAdd BLAST23
Topological domaini239 – 323CytoplasmicBy similarityAdd BLAST85
Transmembranei324 – 344Helical; Name=6By similarityAdd BLAST21
Topological domaini345 – 359ExtracellularBy similarityAdd BLAST15
Transmembranei360 – 381Helical; Name=7By similarityAdd BLAST22
Topological domaini382 – 479CytoplasmicBy similarityAdd BLAST98

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL323
GuidetoPHARMACOLOGYi7

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689561 – 4795-hydroxytryptamine receptor 2BAdd BLAST479

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi127 ↔ 206PROSITE-ProRule annotation
Glycosylationi203N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi349 ↔ 352PROSITE-ProRule annotation
Glycosylationi353N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi396S-palmitoyl cysteineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP30994
PRIDEiP30994

PTM databases

PhosphoSitePlusiP30994

Expressioni

Tissue specificityi

Stomach fundus.2 Publications

Interactioni

Subunit structurei

Interacts (via C-terminus) with MPDZ.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248214, 1 interactor
IntActiP30994, 1 interactor
MINTiP30994
STRINGi10116.ENSRNOP00000023829

Chemistry databases

BindingDBiP30994

Structurei

3D structure databases

ProteinModelPortaliP30994
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 139Agonist bindingBy similarity6
Regioni336 – 340Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi151 – 153DRY motif; important for ligand-induced conformation changesBy similarity3
Motifi211 – 214[DE]RFG motif; may stabilize a conformation that preferentially activates signaling via beta-arrestin family membersBy similarity4
Motifi375 – 379NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity5
Motifi477 – 479PDZ-bindingBy similarity3

Domaini

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
HOGENOMiHOG000240378
HOVERGENiHBG107487
InParanoidiP30994
KOiK04157
PhylomeDBiP30994

Family and domain databases

InterProiView protein in InterPro
IPR000482 5HT2B_rcpt
IPR002231 5HT_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR24247:SF31 PTHR24247:SF31, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00651 5HT2BRECEPTR
PR01101 5HTRECEPTOR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P30994-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASSYKMSEQ STISEHILQK TCDHLILTDR SGLKAESAAE EMKQTAENQG
60 70 80 90 100
NTVHWAALLI FAVIIPTIGG NILVILAVSL EKRLQYATNY FLMSLAVADL
110 120 130 140 150
LVGLFVMPIA LLTIMFEATW PLPLALCPAW LFLDVLFSTA SIMHLCAISL
160 170 180 190 200
DRYIAIKKPI QANQCNSRTT AFVKITVVWL ISIGIAIPVP IKGIEADVVN
210 220 230 240 250
AHNITCELTK DRFGSFMLFG SLAAFFAPLT IMIVTYFLTI HALRKKAYLV
260 270 280 290 300
RNRPPQRLTR WTVSTVLQRE DSSFSSPEKM VMLDGSHKDK ILPNSTDETL
310 320 330 340 350
MRRMSSAGKK PAQTISNEQR ASKVLGIVFL FFLLMWCPFF ITNVTLALCD
360 370 380 390 400
SCNQTTLKTL LQIFVWVGYV SSGVNPLIYT LFNKTFREAF GRYITCNYQA
410 420 430 440 450
TKSVKVLRKC SSTLYFGNSM VENSKFFTKH GIRNGINPAM YQSPVRLRSS
460 470
TIQSSSIILL NTFLTENDGD KVEDQVSYI
Length:479
Mass (Da):53,652
Last modified:July 1, 1993 - v1
Checksum:i17FFC73213B42038
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V8A0G3V8A0_RAT
5-hydroxytryptamine (Serotonin) rec...
Htr2b rCG_23770
479Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281V → A (PubMed:1331748).Curated1
Sequence conflicti296T → I no nucleotide entry (PubMed:1331748).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66842 mRNA Translation: CAA47318.1
PIRiS23562
RefSeqiNP_058946.1, NM_017250.1
UniGeneiRn.10425

Genome annotation databases

GeneIDi29581
KEGGirno:29581
UCSCiRGD:61801 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66842 mRNA Translation: CAA47318.1
PIRiS23562
RefSeqiNP_058946.1, NM_017250.1
UniGeneiRn.10425

3D structure databases

ProteinModelPortaliP30994
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248214, 1 interactor
IntActiP30994, 1 interactor
MINTiP30994
STRINGi10116.ENSRNOP00000023829

Chemistry databases

BindingDBiP30994
ChEMBLiCHEMBL323
GuidetoPHARMACOLOGYi7

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSitePlusiP30994

Proteomic databases

PaxDbiP30994
PRIDEiP30994

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29581
KEGGirno:29581
UCSCiRGD:61801 rat

Organism-specific databases

CTDi3357
RGDi61801 Htr2b

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
HOGENOMiHOG000240378
HOVERGENiHBG107487
InParanoidiP30994
KOiK04157
PhylomeDBiP30994

Miscellaneous databases

PROiPR:P30994

Family and domain databases

InterProiView protein in InterPro
IPR000482 5HT2B_rcpt
IPR002231 5HT_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR24247:SF31 PTHR24247:SF31, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00651 5HT2BRECEPTR
PR01101 5HTRECEPTOR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei5HT2B_RAT
AccessioniPrimary (citable) accession number: P30994
Secondary accession number(s): Q9QW44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 12, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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