UniProtKB - P30994 (5HT2B_RAT)
5-hydroxytryptamine receptor 2B
Htr2b
Functioni
G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:1505525, PubMed:1331748).
Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances (PubMed:22525520).
Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors (PubMed:1505525, PubMed:1331748).
Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores (By similarity).
Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity).
May play a role in the perception of pain (PubMed:22525520).
By similarity3 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 208 | Hydrophobic barrier that decreases the speed of ligand binding and dissociationBy similarity | 1 |
GO - Molecular functioni
- G-protein alpha-subunit binding Source: UniProtKB
- G protein-coupled serotonin receptor activity Source: UniProtKB
- Gq/11-coupled serotonin receptor activity Source: RGD
- GTPase activator activity Source: UniProtKB
- neurotransmitter receptor activity Source: GO_Central
- serotonin binding Source: RGD
GO - Biological processi
- activation of phospholipase C activity Source: RGD
- behavior Source: UniProtKB-KW
- calcium-mediated signaling Source: UniProtKB
- cardiac muscle hypertrophy Source: UniProtKB
- cellular calcium ion homeostasis Source: RGD
- cellular response to amine stimulus Source: RGD
- cellular response to serotonin Source: RGD
- cellular response to temperature stimulus Source: UniProtKB
- cGMP-mediated signaling Source: UniProtKB
- chemical synaptic transmission Source: GO_Central
- embryonic morphogenesis Source: UniProtKB
- ERK1 and ERK2 cascade Source: UniProtKB
- G protein-coupled receptor internalization Source: RGD
- G protein-coupled receptor signaling pathway Source: UniProtKB
- G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
- G protein-coupled serotonin receptor signaling pathway Source: UniProtKB
- heart development Source: RGD
- heart morphogenesis Source: UniProtKB
- hepatic stellate cell activation Source: RGD
- inositol phosphate metabolic process Source: RGD
- intestine smooth muscle contraction Source: UniProtKB
- ion transmembrane transport Source: RGD
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of autophagy Source: UniProtKB
- negative regulation of cell death Source: UniProtKB
- neural crest cell differentiation Source: UniProtKB
- neural crest cell migration Source: UniProtKB
- phosphatidylinositol 3-kinase signaling Source: UniProtKB
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: GO_Central
- phospholipase C-activating serotonin receptor signaling pathway Source: RGD
- phosphorylation Source: UniProtKB
- positive regulation of cell division Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- positive regulation of cytokine production Source: UniProtKB
- positive regulation of endothelial cell proliferation Source: UniProtKB
- positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
- positive regulation of MAP kinase activity Source: RGD
- positive regulation of nitric-oxide synthase activity Source: UniProtKB
- positive regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
- protein kinase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
- protein kinase C signaling Source: UniProtKB
- regulation of behavior Source: UniProtKB
- release of sequestered calcium ion into cytosol Source: UniProtKB
- response to xenobiotic stimulus Source: RGD
- vasoconstriction Source: UniProtKB
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Biological process | Behavior |
Enzyme and pathway databases
Reactomei | R-RNO-390666, Serotonin receptors R-RNO-416476, G alpha (q) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: 5-hydroxytryptamine receptor 2BShort name: 5-HT-2B Short name: 5-HT2B Alternative name(s): 5-HT-2F1 Publication Serotonin receptor 2B Stomach fundus serotonin receptor1 Publication |
Gene namesi | Name:Htr2b Synonyms:Srl |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 61801, Htr2b |
Subcellular locationi
Plasma membrane
- Cell membrane 3 Publications; Multi-pass membrane protein 1 Publication
Other locations
- synaptosome By similarity
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: RGD
- dendrite Source: RGD
- G protein-coupled serotonin receptor complex Source: RGD
- membrane Source: RGD
- neuronal cell body Source: RGD
- synapse Source: UniProtKB-SubCell
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 55 | ExtracellularBy similarityAdd BLAST | 55 | |
Transmembranei | 56 – 78 | Helical; Name=1By similarityAdd BLAST | 23 | |
Topological domaini | 79 – 89 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 90 – 112 | Helical; Name=2By similarityAdd BLAST | 23 | |
Topological domaini | 113 – 128 | ExtracellularBy similarityAdd BLAST | 16 | |
Transmembranei | 129 – 150 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 151 – 170 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 171 – 191 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 192 – 215 | ExtracellularBy similarityAdd BLAST | 24 | |
Transmembranei | 216 – 238 | Helical; Name=5By similarityAdd BLAST | 23 | |
Topological domaini | 239 – 323 | CytoplasmicBy similarityAdd BLAST | 85 | |
Transmembranei | 324 – 344 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 345 – 359 | ExtracellularBy similarityAdd BLAST | 15 | |
Transmembranei | 360 – 381 | Helical; Name=7By similarityAdd BLAST | 22 | |
Topological domaini | 382 – 479 | CytoplasmicBy similarityAdd BLAST | 98 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Synapse, SynaptosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068956 | 1 – 479 | 5-hydroxytryptamine receptor 2BAdd BLAST | 479 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 127 ↔ 206 | PROSITE-ProRule annotation | ||
Glycosylationi | 203 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 349 ↔ 352 | PROSITE-ProRule annotation | ||
Glycosylationi | 353 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 396 | S-palmitoyl cysteineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, PalmitateProteomic databases
PaxDbi | P30994 |
PTM databases
GlyGeni | P30994, 2 sites |
PhosphoSitePlusi | P30994 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts (via C-terminus) with MPDZ.
By similarityGO - Molecular functioni
- G-protein alpha-subunit binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 248214, 1 interactor |
IntActi | P30994, 1 interactor |
MINTi | P30994 |
STRINGi | 10116.ENSRNOP00000023829 |
Chemistry databases
BindingDBi | P30994 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 134 – 139 | Agonist bindingBy similarity | 6 | |
Regioni | 336 – 340 | Agonist bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 151 – 153 | DRY motif; important for ligand-induced conformation changesBy similarity | 3 | |
Motifi | 211 – 214 | [DE]RFG motif; may stabilize a conformation that preferentially activates signaling via beta-arrestin family membersBy similarity | 4 | |
Motifi | 375 – 379 | NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity | 5 | |
Motifi | 477 – 479 | PDZ-bindingBy similarity | 3 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P30994 |
PhylomeDBi | P30994 |
Family and domain databases
InterProi | View protein in InterPro IPR000482, 5HT2B_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF31, PTHR24247:SF31, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00651, 5HT2BRECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MASSYKMSEQ STISEHILQK TCDHLILTDR SGLKAESAAE EMKQTAENQG
60 70 80 90 100
NTVHWAALLI FAVIIPTIGG NILVILAVSL EKRLQYATNY FLMSLAVADL
110 120 130 140 150
LVGLFVMPIA LLTIMFEATW PLPLALCPAW LFLDVLFSTA SIMHLCAISL
160 170 180 190 200
DRYIAIKKPI QANQCNSRTT AFVKITVVWL ISIGIAIPVP IKGIEADVVN
210 220 230 240 250
AHNITCELTK DRFGSFMLFG SLAAFFAPLT IMIVTYFLTI HALRKKAYLV
260 270 280 290 300
RNRPPQRLTR WTVSTVLQRE DSSFSSPEKM VMLDGSHKDK ILPNSTDETL
310 320 330 340 350
MRRMSSAGKK PAQTISNEQR ASKVLGIVFL FFLLMWCPFF ITNVTLALCD
360 370 380 390 400
SCNQTTLKTL LQIFVWVGYV SSGVNPLIYT LFNKTFREAF GRYITCNYQA
410 420 430 440 450
TKSVKVLRKC SSTLYFGNSM VENSKFFTKH GIRNGINPAM YQSPVRLRSS
460 470
TIQSSSIILL NTFLTENDGD KVEDQVSYI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V8A0 | G3V8A0_RAT | 5-hydroxytryptamine receptor 2B | Htr2b rCG_23770 | 479 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 281 | V → A (PubMed:1331748).Curated | 1 | |
Sequence conflicti | 296 | T → I no nucleotide entry (PubMed:1331748).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66842 mRNA Translation: CAA47318.1 |
PIRi | S23562 |
RefSeqi | NP_058946.1, NM_017250.1 |
Genome annotation databases
GeneIDi | 29581 |
KEGGi | rno:29581 |
UCSCi | RGD:61801, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66842 mRNA Translation: CAA47318.1 |
PIRi | S23562 |
RefSeqi | NP_058946.1, NM_017250.1 |
3D structure databases
SMRi | P30994 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248214, 1 interactor |
IntActi | P30994, 1 interactor |
MINTi | P30994 |
STRINGi | 10116.ENSRNOP00000023829 |
Chemistry databases
BindingDBi | P30994 |
ChEMBLi | CHEMBL323 |
DrugCentrali | P30994 |
GuidetoPHARMACOLOGYi | 7 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P30994, 2 sites |
PhosphoSitePlusi | P30994 |
Proteomic databases
PaxDbi | P30994 |
Genome annotation databases
GeneIDi | 29581 |
KEGGi | rno:29581 |
UCSCi | RGD:61801, rat |
Organism-specific databases
CTDi | 3357 |
RGDi | 61801, Htr2b |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
InParanoidi | P30994 |
PhylomeDBi | P30994 |
Enzyme and pathway databases
Reactomei | R-RNO-390666, Serotonin receptors R-RNO-416476, G alpha (q) signalling events |
Miscellaneous databases
PROi | PR:P30994 |
Family and domain databases
InterProi | View protein in InterPro IPR000482, 5HT2B_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF31, PTHR24247:SF31, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00651, 5HT2BRECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 5HT2B_RAT | |
Accessioni | P30994Primary (citable) accession number: P30994 Secondary accession number(s): Q9QW44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families