Htr2b

Functionⁱ

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:1505525, PubMed:1331748). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances (PubMed:22525520). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors (PubMed:1505525, PubMed:1331748). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca²⁺ ions from intracellular stores (By similarity). Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity. Plays a role in the regulation of behavior, including impulsive behavior. Required for normal proliferation of embryonic cardiac myocytes and normal heart development. Protects cardiomyocytes against apoptosis. Plays a role in the adaptation of pulmonary arteries to chronic hypoxia. Plays a role in vasoconstriction. Required for normal osteoblast function and proliferation, and for maintaining normal bone density. Required for normal proliferation of the interstitial cells of Cajal in the intestine (By similarity). May play a role in the perception of pain (PubMed:22525520).By similarity3 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	208	Hydrophobic barrier that decreases the speed of ligand binding and dissociationBy similarity		1

GO - Molecular functionⁱ

drug binding Source: UniProtKB
G-protein alpha-subunit binding Source: UniProtKB
G-protein coupled serotonin receptor activity Source: UniProtKB
GTPase activator activity Source: UniProtKB
neurotransmitter receptor activity Source: GO_Central
serotonin binding
Source: RGD
Inferred from direct assayⁱ
- 1505525

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

behavior Source: UniProtKB-KW
calcium-mediated signaling Source: UniProtKB
cardiac muscle hypertrophy Source: UniProtKB
cellular response to amine stimulus
Source: RGD
Inferred from direct assayⁱ
- 1505525
cellular response to serotonin
Source: RGD
Inferred from direct assayⁱ
- 1505525
cellular response to temperature stimulus Source: UniProtKB
cGMP-mediated signaling Source: UniProtKB
embryonic morphogenesis Source: UniProtKB
ERK1 and ERK2 cascade Source: UniProtKB
G-protein coupled receptor signaling pathway Source: UniProtKB
heart morphogenesis Source: UniProtKB
hepatic stellate cell activation
Source: RGD
Inferred from expression patternⁱ
- 16936262
inositol phosphate metabolic process
Source: RGD
Inferred from expression patternⁱ
- 1331748
intestine smooth muscle contraction Source: UniProtKB
ion transmembrane transport
Source: RGD
Inferred from direct assayⁱ
- 1505525
negative regulation of apoptotic process Source: UniProtKB
negative regulation of autophagy Source: UniProtKB
negative regulation of cell death Source: UniProtKB
neural crest cell differentiation Source: UniProtKB
neural crest cell migration Source: UniProtKB
phosphatidylinositol 3-kinase signaling Source: UniProtKB
phospholipase C-activating G-protein coupled receptor signaling pathway Source: GO_Central
phosphorylation Source: UniProtKB
positive regulation of cell division Source: UniProtKB
positive regulation of cell proliferation Source: UniProtKB
positive regulation of cytokine production Source: UniProtKB
positive regulation of cytokine secretion Source: UniProtKB
positive regulation of endothelial cell proliferation Source: UniProtKB
positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
positive regulation of nitric-oxide synthase activity Source: UniProtKB
positive regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
protein kinase C signaling Source: UniProtKB
regulation of behavior Source: UniProtKB
release of sequestered calcium ion into cytosol Source: UniProtKB
response to drug Source: UniProtKB
serotonin receptor signaling pathway Source: UniProtKB
vasoconstriction Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	G-protein coupled receptor, Receptor, Transducer
Biological process	Behavior

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 5-hydroxytryptamine receptor 2B Short name: 5-HT-2B Short name: 5-HT2B Alternative name(s): 5-HT-2F1 Publication Serotonin receptor 2B Stomach fundus serotonin receptor1 Publication
Gene namesⁱ	Name:Htr2b Synonyms:Srl
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Unplaced

Organism-specific databases

Rat genome database More...RGDⁱ	61801 Htr2b

RGDⁱ

61801 Htr2b

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 55	ExtracellularBy similarityAdd BLAST	55
Transmembraneⁱ	56 – 78	Helical; Name=1By similarityAdd BLAST	23
Topological domainⁱ	79 – 89	CytoplasmicBy similarityAdd BLAST	11
Transmembraneⁱ	90 – 112	Helical; Name=2By similarityAdd BLAST	23
Topological domainⁱ	113 – 128	ExtracellularBy similarityAdd BLAST	16
Transmembraneⁱ	129 – 150	Helical; Name=3By similarityAdd BLAST	22
Topological domainⁱ	151 – 170	CytoplasmicBy similarityAdd BLAST	20
Transmembraneⁱ	171 – 191	Helical; Name=4By similarityAdd BLAST	21
Topological domainⁱ	192 – 215	ExtracellularBy similarityAdd BLAST	24
Transmembraneⁱ	216 – 238	Helical; Name=5By similarityAdd BLAST	23
Topological domainⁱ	239 – 323	CytoplasmicBy similarityAdd BLAST	85
Transmembraneⁱ	324 – 344	Helical; Name=6By similarityAdd BLAST	21
Topological domainⁱ	345 – 359	ExtracellularBy similarityAdd BLAST	15
Transmembraneⁱ	360 – 381	Helical; Name=7By similarityAdd BLAST	22
Topological domainⁱ	382 – 479	CytoplasmicBy similarityAdd BLAST	98

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Membrane, Synapse, Synaptosome

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL323
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	7

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000068956	1 – 479	5-hydroxytryptamine receptor 2BAdd BLAST		479

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	127 ↔ 206	PROSITE-ProRule annotation
Glycosylationⁱ	203	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	349 ↔ 352	PROSITE-ProRule annotation
Glycosylationⁱ	353	N-linked (GlcNAc...) asparagineSequence analysis	1
Lipidationⁱ	396	S-palmitoyl cysteineSequence analysis	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbⁱ	P30994
PRIDEⁱ	P30994

PTM databases

PhosphoSitePlusⁱ	P30994

PhosphoSitePlusⁱ

P30994

Expressionⁱ

Tissue specificityⁱ

Stomach fundus.2 Publications

Interactionⁱ

Subunit structureⁱ

Interacts (via C-terminus) with MPDZ.By similarity

GO - Molecular functionⁱ

G-protein alpha-subunit binding Source: UniProtKB

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	248214, 1 interactor
IntActⁱ	P30994, 1 interactor
Molecular INTeraction database More...MINTⁱ	P30994
STRINGⁱ	10116.ENSRNOP00000023829

Chemistry databases

BindingDBⁱ

P30994

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P30994
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	134 – 139	Agonist bindingBy similarity		6
Regionⁱ	336 – 340	Agonist bindingBy similarity		5

Motif

Feature key	Position(s)	DescriptionActions	Length
Motifⁱ	151 – 153	DRY motif; important for ligand-induced conformation changesBy similarity	3
Motifⁱ	211 – 214	[DE]RFG motif; may stabilize a conformation that preferentially activates signaling via beta-arrestin family membersBy similarity	4
Motifⁱ	375 – 379	NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity	5
Motifⁱ	477 – 479	PDZ-bindingBy similarity	3

Domainⁱ

Ligands are bound in a hydrophobic pocket formed by the transmembrane helices.By similarity

Sequence similaritiesⁱ

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG3656 Eukaryota ENOG410XRW9 LUCA
HOGENOMⁱ	HOG000240378
HOVERGENⁱ	HBG107487
InParanoidⁱ	P30994
KEGG Orthology (KO) More...KOⁱ	K04157
PhylomeDBⁱ	P30994

Family and domain databases

InterProⁱ	View protein in InterPro IPR000482 5HT2B_rcpt IPR002231 5HT_rcpt IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM
PANTHERⁱ	PTHR24247:SF31 PTHR24247:SF31, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00651 5HT2BRECEPTR PR01101 5HTRECEPTOR PR00237 GPCRRHODOPSN
SMARTⁱ	View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P30994-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MASSYKMSEQ STISEHILQK TCDHLILTDR SGLKAESAAE EMKQTAENQG 
        60         70         80         90        100
NTVHWAALLI FAVIIPTIGG NILVILAVSL EKRLQYATNY FLMSLAVADL 
       110        120        130        140        150
LVGLFVMPIA LLTIMFEATW PLPLALCPAW LFLDVLFSTA SIMHLCAISL 
       160        170        180        190        200
DRYIAIKKPI QANQCNSRTT AFVKITVVWL ISIGIAIPVP IKGIEADVVN 
       210        220        230        240        250
AHNITCELTK DRFGSFMLFG SLAAFFAPLT IMIVTYFLTI HALRKKAYLV 
       260        270        280        290        300
RNRPPQRLTR WTVSTVLQRE DSSFSSPEKM VMLDGSHKDK ILPNSTDETL 
       310        320        330        340        350
MRRMSSAGKK PAQTISNEQR ASKVLGIVFL FFLLMWCPFF ITNVTLALCD 
       360        370        380        390        400
SCNQTTLKTL LQIFVWVGYV SSGVNPLIYT LFNKTFREAF GRYITCNYQA 
       410        420        430        440        450
TKSVKVLRKC SSTLYFGNSM VENSKFFTKH GIRNGINPAM YQSPVRLRSS 
       460        470 
TIQSSSIILL NTFLTENDGD KVEDQVSYI

Length:479

Mass (Da):53,652

Last modified:July 1, 1993 - v1

Checksum:ⁱ17FFC73213B42038

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	G3V8A0	G3V8A0_RAT		5-hydroxytryptamine (Serotonin) rec... 5-hydroxytryptamine (Serotonin) receptor 2B (5-hydroxytryptamine receptor 2B)	Htr2b rCG_23770	479	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	281	V → A (PubMed:1331748).Curated		1
Sequence conflictⁱ	296	T → I no nucleotide entry (PubMed:1331748).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X66842 mRNA Translation: CAA47318.1
PIRⁱ	S23562
NCBI Reference Sequences More...RefSeqⁱ	NP_058946.1, NM_017250.1
UniGeneⁱ	Rn.10425

Genome annotation databases

GeneIDⁱ	29581
KEGGⁱ	rno:29581
UCSC genome browser More...UCSCⁱ	RGD:61801 rat

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P30994	5-hydroxytryptamine (Serotonin) receptor 2B		RAT		479	UniRef90_P30994

Protein	Similar proteins		Species	Score	Length	Source
P30994	5-hydroxytryptamine receptor 2B (Fragment)		CAVPO		96	UniRef50_P30994
5-hydroxytryptamine (Serotonin) receptor 2B		RAT		479
Uncharacterized protein		RHIBE		414
5-hydroxytryptamine receptor 2B		PIG		414
5-hydroxytryptamine receptor 2B variant b (Fragment)		HUMAN		53
+63

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X66842 mRNA Translation: CAA47318.1
PIRⁱ	S23562
RefSeqⁱ	NP_058946.1, NM_017250.1
UniGeneⁱ	Rn.10425

3D structure databases

ProteinModelPortalⁱ	P30994
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	248214, 1 interactor
IntActⁱ	P30994, 1 interactor
MINTⁱ	P30994
STRINGⁱ	10116.ENSRNOP00000023829

Chemistry databases

BindingDBⁱ	P30994
ChEMBLⁱ	CHEMBL323
GuidetoPHARMACOLOGYⁱ	7

Protein family/group databases

GPCRDBⁱ	Search...

GPCRDBⁱ

Search...

PTM databases

PhosphoSitePlusⁱ	P30994

PhosphoSitePlusⁱ

P30994

Proteomic databases

PaxDbⁱ	P30994
PRIDEⁱ	P30994

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	29581
KEGGⁱ	rno:29581
UCSCⁱ	RGD:61801 rat

Organism-specific databases

CTDⁱ	3357
RGDⁱ	61801 Htr2b

Phylogenomic databases

eggNOGⁱ	KOG3656 Eukaryota ENOG410XRW9 LUCA
HOGENOMⁱ	HOG000240378
HOVERGENⁱ	HBG107487
InParanoidⁱ	P30994
KOⁱ	K04157
PhylomeDBⁱ	P30994

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P30994

PROⁱ

PR:P30994

Family and domain databases

InterProⁱ	View protein in InterPro IPR000482 5HT2B_rcpt IPR002231 5HT_rcpt IPR000276 GPCR_Rhodpsn IPR017452 GPCR_Rhodpsn_7TM
PANTHERⁱ	PTHR24247:SF31 PTHR24247:SF31, 1 hit
Pfamⁱ	View protein in Pfam PF00001 7tm_1, 1 hit
PRINTSⁱ	PR00651 5HT2BRECEPTR PR01101 5HTRECEPTOR PR00237 GPCRRHODOPSN
SMARTⁱ	View protein in SMART SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEⁱ	View protein in PROSITE PS00237 G_PROTEIN_RECEP_F1_1, 1 hit PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	5HT2B_RAT
Accessionⁱ	P30994Primary (citable) accession number: P30994 Secondary accession number(s): Q9QW44
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
	Last sequence update:	July 1, 1993
	Last modified:	September 12, 2018
	This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P30994 (5HT2B_RAT)

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5-hydroxytryptamine receptor 2B

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ