UniProtKB - P30966 (5HT5A_MOUSE)
Protein
5-hydroxytryptamine receptor 5A
Gene
Htr5a
Organism
Mus musculus (Mouse)
Status
Functioni
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins.
GO - Molecular functioni
- G protein-coupled receptor activity Source: GO_Central
- G protein-coupled serotonin receptor activity Source: MGI
- neurotransmitter receptor activity Source: GO_Central
- serotonin binding Source: GO_Central
GO - Biological processi
- adenylate cyclase-inhibiting serotonin receptor signaling pathway Source: GO_Central
- cAMP-mediated signaling Source: MGI
- chemical synaptic transmission Source: GO_Central
- G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: MGI
- hippocampus development Source: Ensembl
- response to estradiol Source: Ensembl
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-390666, Serotonin receptors R-MMU-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: 5-hydroxytryptamine receptor 5AShort name: 5-HT-5 Short name: 5-HT-5A Short name: 5-HT5A Alternative name(s): Serotonin receptor 5A |
Gene namesi | Name:Htr5a Synonyms:5ht5a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96283, Htr5a |
Subcellular locationi
Plasma membrane
Endoplasmic reticulum
- rough endoplasmic reticulum Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic specialization membrane Source: MGI
Other locations
- dendrite Source: MGI
- perikaryon Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 40 | ExtracellularBy similarityAdd BLAST | 40 | |
Transmembranei | 41 – 63 | Helical; Name=1By similarityAdd BLAST | 23 | |
Topological domaini | 64 – 78 | CytoplasmicBy similarityAdd BLAST | 15 | |
Transmembranei | 79 – 99 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 100 – 115 | ExtracellularBy similarityAdd BLAST | 16 | |
Transmembranei | 116 – 137 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 138 – 158 | CytoplasmicBy similarityAdd BLAST | 21 | |
Transmembranei | 159 – 181 | Helical; Name=4By similarityAdd BLAST | 23 | |
Topological domaini | 182 – 198 | ExtracellularBy similarityAdd BLAST | 17 | |
Transmembranei | 199 – 219 | Helical; Name=5By similarityAdd BLAST | 21 | |
Topological domaini | 220 – 282 | CytoplasmicBy similarityAdd BLAST | 63 | |
Transmembranei | 283 – 303 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 304 – 320 | ExtracellularBy similarityAdd BLAST | 17 | |
Transmembranei | 321 – 341 | Helical; Name=7By similarityAdd BLAST | 21 | |
Topological domaini | 342 – 357 | CytoplasmicBy similarityAdd BLAST | 16 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000068970 | 1 – 357 | 5-hydroxytryptamine receptor 5AAdd BLAST | 357 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 6 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 21 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 120 ↔ 192 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P30966 |
PRIDEi | P30966 |
PTM databases
GlyGeni | P30966, 2 sites |
PhosphoSitePlusi | P30966 |
Expressioni
Tissue specificityi
Expressed predominantly in the central nervous system; in the cerebral cortex, hippocampus, habenula, olfactory bulb and granular layer of the cerebellum.
Gene expression databases
Bgeei | ENSMUSG00000039106, Expressed in adult mammalian kidney and 70 other tissues |
Genevisiblei | P30966, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038884 |
Chemistry databases
BindingDBi | P30966 |
Miscellaneous databases
RNActi | P30966, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222334 |
HOGENOMi | CLU_009579_11_6_1 |
InParanoidi | P30966 |
OMAi | RRISNIM |
OrthoDBi | 817833at2759 |
TreeFami | TF316350 |
Family and domain databases
InterProi | View protein in InterPro IPR001397, 5HT5A_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF108, PTHR24247:SF108, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00518, 5HT5ARECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P30966-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLPVNLTSF SLSTPSSLEP NRSLDTEVLR PSRPFLSAFR VLVLTLLGFL
60 70 80 90 100
AAATFTWNLL VLATILKVRT FHRVPHNLVA SMAISDVLVA VLVMPLSLVH
110 120 130 140 150
ELSGRRWQLG RRLCQLWIAC DVLCCTASIW NVTAIALDRY WSITRHLEYT
160 170 180 190 200
LRTRKRVSNV MILLTWALST VISLAPLLFG WGETYSEPSE ECQVSREPSY
210 220 230 240 250
TVFSTVGAFY LPLCVVLFVY WKIYRAAKFR MGSRKTNSVS PVPEAVEVKN
260 270 280 290 300
ATQHPQMVFT VRHATVTFQT EGDTWREQKE QRAALMVGIL IGVFVLCWFP
310 320 330 340 350
FFVTELISPL CSWDVPAIWK SIFLWLGYSN SFFNPLIYTA FNRSYSSAFK
VFFSKQQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 214 – 215 | CV → WL in CAA79155 (PubMed:1464308).Curated | 2 | |
Sequence conflicti | 261 | V → A in CAA79155 (PubMed:1464308).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z18278 Genomic DNA Translation: CAA79155.1 AK141636 mRNA Translation: BAE24778.1 CH466524 Genomic DNA Translation: EDL37224.1 BC115911 mRNA Translation: AAI15912.1 BC116654 mRNA Translation: AAI16655.1 |
CCDSi | CCDS19141.1 |
PIRi | S28058 |
RefSeqi | NP_032340.2, NM_008314.2 |
Genome annotation databases
Ensembli | ENSMUST00000036227; ENSMUSP00000038884; ENSMUSG00000039106 |
GeneIDi | 15563 |
KEGGi | mmu:15563 |
UCSCi | uc008wtt.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z18278 Genomic DNA Translation: CAA79155.1 AK141636 mRNA Translation: BAE24778.1 CH466524 Genomic DNA Translation: EDL37224.1 BC115911 mRNA Translation: AAI15912.1 BC116654 mRNA Translation: AAI16655.1 |
CCDSi | CCDS19141.1 |
PIRi | S28058 |
RefSeqi | NP_032340.2, NM_008314.2 |
3D structure databases
SMRi | P30966 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038884 |
Chemistry databases
BindingDBi | P30966 |
ChEMBLi | CHEMBL3597 |
DrugCentrali | P30966 |
GuidetoPHARMACOLOGYi | 10 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P30966, 2 sites |
PhosphoSitePlusi | P30966 |
Proteomic databases
PaxDbi | P30966 |
PRIDEi | P30966 |
Protocols and materials databases
Antibodypediai | 18848, 345 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000036227; ENSMUSP00000038884; ENSMUSG00000039106 |
GeneIDi | 15563 |
KEGGi | mmu:15563 |
UCSCi | uc008wtt.1, mouse |
Organism-specific databases
CTDi | 3361 |
MGIi | MGI:96283, Htr5a |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT01010000222334 |
HOGENOMi | CLU_009579_11_6_1 |
InParanoidi | P30966 |
OMAi | RRISNIM |
OrthoDBi | 817833at2759 |
TreeFami | TF316350 |
Enzyme and pathway databases
Reactomei | R-MMU-390666, Serotonin receptors R-MMU-418594, G alpha (i) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 15563, 1 hit in 17 CRISPR screens |
PROi | PR:P30966 |
RNActi | P30966, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039106, Expressed in adult mammalian kidney and 70 other tissues |
Genevisiblei | P30966, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001397, 5HT5A_rcpt IPR002231, 5HT_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24247:SF108, PTHR24247:SF108, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00518, 5HT5ARECEPTR PR01101, 5HTRECEPTOR PR00237, GPCRRHODOPSN |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 5HT5A_MOUSE | |
Accessioni | P30966Primary (citable) accession number: P30966 Secondary accession number(s): Q3URB0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries