UniProtKB - P30926 (ACHB4_HUMAN)
Neuronal acetylcholine receptor subunit beta-4
CHRNB4
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 82 | Key residue that facilitates effective access of the conotoxin BuIA to the channel binding siteBy similarity | 1 | |
Sitei | 134 | Key residue for a low dissociation (K(off)) from the conotoxin BuIABy similarity | 1 |
GO - Molecular functioni
- acetylcholine binding Source: Ensembl
- acetylcholine-gated cation-selective channel activity Source: UniProtKB
- acetylcholine receptor activity Source: UniProtKB
- ligand-gated ion channel activity Source: DFLAT
- neurotransmitter receptor activity Source: GO_Central
- protein-containing complex binding Source: Ensembl
GO - Biological processi
- action potential Source: Ensembl
- behavioral response to nicotine Source: Ensembl
- chemical synaptic transmission Source: GO_Central
- ion transmembrane transport Source: GO_Central
- ion transport Source: UniProtKB
- locomotory behavior Source: Ensembl
- nervous system process Source: GO_Central
- neutrophil degranulation Source: Reactome
- positive regulation of transmission of nerve impulse Source: Ensembl
- regulation of membrane potential Source: GO_Central
- regulation of neurotransmitter secretion Source: UniProtKB
- regulation of smooth muscle contraction Source: Ensembl
- signal transduction Source: UniProtKB
- smooth muscle contraction Source: Ensembl
- synaptic transmission, cholinergic Source: ProtInc
- synaptic transmission involved in micturition Source: UniProtKB
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | P30926 |
Reactomei | R-HSA-629587, Highly sodium permeable postsynaptic acetylcholine nicotinic receptors R-HSA-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-HSA-629597, Highly calcium permeable nicotinic acetylcholine receptors R-HSA-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Neuronal acetylcholine receptor subunit beta-4 |
Gene namesi | Name:CHRNB4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1964, CHRNB4 |
MIMi | 118509, gene |
neXtProti | NX_P30926 |
VEuPathDBi | HostDB:ENSG00000117971.11 |
Subcellular locationi
Plasma Membrane
- acetylcholine-gated channel complex Source: UniProtKB
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: Reactome
- postsynaptic membrane Source: UniProtKB-SubCell
Other locations
- cholinergic synapse Source: Ensembl
- integral component of membrane Source: UniProtKB
- neuron projection Source: GO_Central
- specific granule membrane Source: Reactome
- synapse Source: GO_Central
- tertiary granule membrane Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 236 | ExtracellularSequence analysisAdd BLAST | 215 | |
Transmembranei | 237 – 257 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 258 – 265 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 266 – 286 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 287 – 298 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 299 – 319 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 320 – 460 | CytoplasmicSequence analysisAdd BLAST | 141 | |
Transmembranei | 461 – 481 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 482 – 498 | ExtracellularSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePathology & Biotechi
Organism-specific databases
DisGeNETi | 1143 |
OpenTargetsi | ENSG00000117971 |
PharmGKBi | PA26496 |
Miscellaneous databases
Pharosi | P30926, Tclin |
Chemistry databases
ChEMBLi | CHEMBL1907591 CHEMBL1907594 CHEMBL2109230 CHEMBL3038459 CHEMBL3137273 CHEMBL3137285 CHEMBL3885595 |
DrugBanki | DB00237, Butabarbital DB00514, Dextromethorphan DB07720, Epibatidine DB00898, Ethanol DB00472, Fluoxetine DB01227, Levacetylmethadol DB00184, Nicotine DB01090, Pentolinium |
DrugCentrali | P30926 |
Genetic variation databases
BioMutai | CHRNB4 |
DMDMi | 2506129 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000000389 | 22 – 498 | Neuronal acetylcholine receptor subunit beta-4Add BLAST | 477 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 36 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 93 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 138 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 153 ↔ 167 | By similarity | ||
Glycosylationi | 166 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | P30926 |
MassIVEi | P30926 |
PaxDbi | P30926 |
PeptideAtlasi | P30926 |
PRIDEi | P30926 |
ProteomicsDBi | 20517 54746 [P30926-1] |
PTM databases
GlyGeni | P30926, 4 sites |
iPTMneti | P30926 |
PhosphoSitePlusi | P30926 |
Expressioni
Gene expression databases
Bgeei | ENSG00000117971, Expressed in right testis and 92 other tissues |
ExpressionAtlasi | P30926, baseline and differential |
Genevisiblei | P30926, HS |
Organism-specific databases
HPAi | ENSG00000117971, Group enriched (adrenal gland, lymphoid tissue, retina, testis) |
Interactioni
Subunit structurei
Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-4 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors.
Interacts with RIC3; which is required for proper folding and assembly (PubMed:16120769).
Interacts with LYPD6 (PubMed:27344019). The pentamer alpha3-beta-4 interacts with the conotoxin BuIA (By similarity). The heteropentamer composed of alpha-3 and beta-4 subunits interacts with the alpha-conotoxin ImI (PubMed:15609996).
By similarity3 PublicationsBinary interactionsi
P30926
With | #Exp. | IntAct |
---|---|---|
MEOX2 [Q6FHY5] | 3 | EBI-9009018,EBI-16439278 |
Protein-protein interaction databases
BioGRIDi | 107565, 79 interactors |
ComplexPortali | CPX-210, Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta4 CPX-213, Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta4 CPX-2190, Neuronal nicotinic acetylcholine receptor complex, alpha2-beta4 CPX-2200, Neuronal nicotinic acetylcholine receptor complex, alpha3-beta4 CPX-2203, Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4 |
CORUMi | P30926 |
IntActi | P30926, 3 interactors |
STRINGi | 9606.ENSP00000261751 |
Chemistry databases
BindingDBi | P30926 |
Miscellaneous databases
RNActi | P30926, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P30926 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000158708 |
HOGENOMi | CLU_018074_1_1_1 |
InParanoidi | P30926 |
OMAi | PSKLYGN |
OrthoDBi | 381858at2759 |
PhylomeDBi | P30926 |
TreeFami | TF315605 |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRRAPSLVLF FLVALCGRGN CRVANAEEKL MDDLLNKTRY NNLIRPATSS
60 70 80 90 100
SQLISIKLQL SLAQLISVNE REQIMTTNVW LKQEWTDYRL TWNSSRYEGV
110 120 130 140 150
NILRIPAKRI WLPDIVLYNN ADGTYEVSVY TNLIVRSNGS VLWLPPAIYK
160 170 180 190 200
SACKIEVKYF PFDQQNCTLK FRSWTYDHTE IDMVLMTPTA SMDDFTPSGE
210 220 230 240 250
WDIVALPGRR TVNPQDPSYV DVTYDFIIKR KPLFYTINLI IPCVLTTLLA
260 270 280 290 300
ILVFYLPSDC GEKMTLCISV LLALTFFLLL ISKIVPPTSL DVPLIGKYLM
310 320 330 340 350
FTMVLVTFSI VTSVCVLNVH HRSPSTHTMA PWVKRCFLHK LPTFLFMKRP
360 370 380 390 400
GPDSSPARAF PPSKSCVTKP EATATSTSPS NFYGNSMYFV NPASAASKSP
410 420 430 440 450
AGSTPVAIPR DFWLRSSGRF RQDVQEALEG VSFIAQHMKN DDEDQSVVED
460 470 480 490
WKYVAMVVDR LFLWVFMFVC VLGTVGLFLP PLFQTHAASE GPYAAQRD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH3BU02 | H3BU02_HUMAN | Neuronal acetylcholine receptor sub... | CHRNB4 | 66 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 72 – 73 | EQ → DE in CAA48336 (PubMed:1330682).Curated | 2 | |
Sequence conflicti | 121 | Missing in CAC34819 (Ref. 4) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048174 | 91 | T → I. Corresponds to variant dbSNP:rs12914008Ensembl. | 1 | |
Natural variantiVAR_013241 | 136 | R → W1 PublicationCorresponds to variant dbSNP:rs141876090Ensembl. | 1 | |
Natural variantiVAR_013242 | 140 | S → G1 PublicationCorresponds to variant dbSNP:rs56218866Ensembl. | 1 | |
Natural variantiVAR_013243 | 467 | M → V1 PublicationCorresponds to variant dbSNP:rs61737502Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046674 | 120 – 217 | NADGT…NPQDP → KSLRTGSTWLWWWTGCSCGC SCLCASWALWGSSYRPSSRP MQLLRGPTLPSVTEGPLGCG VRGCEWPGGHFAASFWVVAD EALSKYVSIGHQPHQTSHSR GTGKDGGLGCPL in isoform 2. 1 PublicationAdd BLAST | 98 | |
Alternative sequenceiVSP_046675 | 218 – 498 | Missing in isoform 2. 1 PublicationAdd BLAST | 281 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U62439 mRNA Translation: AAB40117.1 U48861 mRNA Translation: AAA92123.1 Y08416 mRNA Translation: CAA69693.1 AJ306454 , AJ306455, AJ306456, AJ306457, AJ306458, AJ306459 Genomic DNA Translation: CAC34819.1 AF306329 , AF306325, AF306326, AF306327, AF306328 Genomic DNA Translation: AAL02062.1 AC022748 Genomic DNA No translation available. AC067863 Genomic DNA No translation available. BC096080 mRNA Translation: AAH96080.1 BC096081 mRNA Translation: AAH96081.1 BC096083 mRNA Translation: AAH96083.1 BC096082 mRNA Translation: AAH96082.1 AF453877 Genomic DNA Translation: AAL57840.1 X68275 mRNA Translation: CAA48336.1 |
CCDSi | CCDS10306.1 [P30926-1] CCDS58392.1 [P30926-2] |
PIRi | G02421 |
RefSeqi | NP_000741.1, NM_000750.3 [P30926-1] NP_001243496.1, NM_001256567.1 [P30926-2] |
Genome annotation databases
Ensembli | ENST00000261751; ENSP00000261751; ENSG00000117971 [P30926-1] ENST00000412074; ENSP00000416386; ENSG00000117971 [P30926-2] |
GeneIDi | 1143 |
KEGGi | hsa:1143 |
UCSCi | uc002bed.2, human [P30926-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U62439 mRNA Translation: AAB40117.1 U48861 mRNA Translation: AAA92123.1 Y08416 mRNA Translation: CAA69693.1 AJ306454 , AJ306455, AJ306456, AJ306457, AJ306458, AJ306459 Genomic DNA Translation: CAC34819.1 AF306329 , AF306325, AF306326, AF306327, AF306328 Genomic DNA Translation: AAL02062.1 AC022748 Genomic DNA No translation available. AC067863 Genomic DNA No translation available. BC096080 mRNA Translation: AAH96080.1 BC096081 mRNA Translation: AAH96081.1 BC096083 mRNA Translation: AAH96083.1 BC096082 mRNA Translation: AAH96082.1 AF453877 Genomic DNA Translation: AAL57840.1 X68275 mRNA Translation: CAA48336.1 |
CCDSi | CCDS10306.1 [P30926-1] CCDS58392.1 [P30926-2] |
PIRi | G02421 |
RefSeqi | NP_000741.1, NM_000750.3 [P30926-1] NP_001243496.1, NM_001256567.1 [P30926-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ASG | model | - | B/D/E | 262-284 | [»] | |
6PV7 | electron microscopy | 3.34 | B/C/E | 22-361 | [»] | |
B/C/E | 417-498 | [»] | ||||
6PV8 | electron microscopy | 3.87 | B/C/E | 22-361 | [»] | |
B/C/E | 417-498 | [»] | ||||
SMRi | P30926 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107565, 79 interactors |
ComplexPortali | CPX-210, Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha5-beta4 CPX-213, Neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta4 CPX-2190, Neuronal nicotinic acetylcholine receptor complex, alpha2-beta4 CPX-2200, Neuronal nicotinic acetylcholine receptor complex, alpha3-beta4 CPX-2203, Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4 |
CORUMi | P30926 |
IntActi | P30926, 3 interactors |
STRINGi | 9606.ENSP00000261751 |
Chemistry databases
BindingDBi | P30926 |
ChEMBLi | CHEMBL1907591 CHEMBL1907594 CHEMBL2109230 CHEMBL3038459 CHEMBL3137273 CHEMBL3137285 CHEMBL3885595 |
DrugBanki | DB00237, Butabarbital DB00514, Dextromethorphan DB07720, Epibatidine DB00898, Ethanol DB00472, Fluoxetine DB01227, Levacetylmethadol DB00184, Nicotine DB01090, Pentolinium |
DrugCentrali | P30926 |
PTM databases
GlyGeni | P30926, 4 sites |
iPTMneti | P30926 |
PhosphoSitePlusi | P30926 |
Genetic variation databases
BioMutai | CHRNB4 |
DMDMi | 2506129 |
Proteomic databases
EPDi | P30926 |
MassIVEi | P30926 |
PaxDbi | P30926 |
PeptideAtlasi | P30926 |
PRIDEi | P30926 |
ProteomicsDBi | 20517 54746 [P30926-1] |
Protocols and materials databases
Antibodypediai | 15128, 169 antibodies |
Genome annotation databases
Ensembli | ENST00000261751; ENSP00000261751; ENSG00000117971 [P30926-1] ENST00000412074; ENSP00000416386; ENSG00000117971 [P30926-2] |
GeneIDi | 1143 |
KEGGi | hsa:1143 |
UCSCi | uc002bed.2, human [P30926-1] |
Organism-specific databases
CTDi | 1143 |
DisGeNETi | 1143 |
GeneCardsi | CHRNB4 |
HGNCi | HGNC:1964, CHRNB4 |
HPAi | ENSG00000117971, Group enriched (adrenal gland, lymphoid tissue, retina, testis) |
MIMi | 118509, gene |
neXtProti | NX_P30926 |
OpenTargetsi | ENSG00000117971 |
PharmGKBi | PA26496 |
VEuPathDBi | HostDB:ENSG00000117971.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000158708 |
HOGENOMi | CLU_018074_1_1_1 |
InParanoidi | P30926 |
OMAi | PSKLYGN |
OrthoDBi | 381858at2759 |
PhylomeDBi | P30926 |
TreeFami | TF315605 |
Enzyme and pathway databases
PathwayCommonsi | P30926 |
Reactomei | R-HSA-629587, Highly sodium permeable postsynaptic acetylcholine nicotinic receptors R-HSA-629594, Highly calcium permeable postsynaptic nicotinic acetylcholine receptors R-HSA-629597, Highly calcium permeable nicotinic acetylcholine receptors R-HSA-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 1143, 3 hits in 871 CRISPR screens |
GeneWikii | CHRNB4 |
GenomeRNAii | 1143 |
Pharosi | P30926, Tclin |
PROi | PR:P30926 |
RNActi | P30926, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000117971, Expressed in right testis and 92 other tissues |
ExpressionAtlasi | P30926, baseline and differential |
Genevisiblei | P30926, HS |
Family and domain databases
Gene3Di | 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
TIGRFAMsi | TIGR00860, LIC, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACHB4_HUMAN | |
Accessioni | P30926Primary (citable) accession number: P30926 Secondary accession number(s): A4FTX5 Q9BQR4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 203 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families