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Protein

Macrophage migration inhibitory factor

Gene

Mif

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).By similarity

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Proton acceptor; via imino nitrogenBy similarity1
Binding sitei33SubstrateBy similarity1
Binding sitei65Substrate; via amide nitrogenBy similarity1
Binding sitei98SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • brain development Source: RGD
  • brain renin-angiotensin system Source: RGD
  • cellular response to hydrogen peroxide Source: RGD
  • cellular response to hypoxia Source: RGD
  • drinking behavior Source: RGD
  • hair follicle development Source: RGD
  • inflammatory response Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • positive regulation of acute inflammatory response Source: RGD
  • positive regulation of axon regeneration Source: RGD
  • positive regulation of glucose import Source: RGD
  • positive regulation of glycolytic process Source: RGD
  • positive regulation of immune response Source: RGD
  • positive regulation of potassium ion transport Source: RGD
  • positive regulation of smooth muscle cell migration Source: RGD
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to glucocorticoid Source: RGD
  • response to heat Source: RGD
  • response to hormone Source: RGD
  • response to inorganic substance Source: RGD
  • response to insulin Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to menaquinone Source: RGD
  • response to progesterone Source: RGD
  • response to vitamin E Source: RGD
  • skin development Source: RGD
  • spermatogenesis Source: RGD
  • wound healing Source: RGD

Keywordsi

Molecular functionCytokine, Isomerase
Biological processImmunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiR-RNO-202733 Cell surface interactions at the vascular wall
R-RNO-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
Glutathione-binding 13 kDa protein
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
Name:Mif
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi621163 Mif

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001580682 – 115Macrophage migration inhibitory factorAdd BLAST114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78N6-acetyllysine; alternateBy similarity1
Modified residuei78N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP30904
PRIDEiP30904

PTM databases

iPTMnetiP30904
PhosphoSitePlusiP30904
SwissPalmiP30904

Expressioni

Tissue specificityi

Expressed in a wide variety of organs including brain, spleen, liver, muscle and kidney.

Gene expression databases

BgeeiENSRNOG00000006589
ExpressionAtlasiP30904 differential
GenevisibleiP30904 RN

Interactioni

Subunit structurei

Homotrimer. Interacts with BNIPL (By similarity). Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP30904, 3 interactors
MINTiP30904
STRINGi10116.ENSRNOP00000008608

Structurei

Secondary structure

1115
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 14Combined sources3
Helixi19 – 31Combined sources13
Helixi35 – 37Combined sources3
Beta strandi39 – 43Combined sources5
Beta strandi58 – 66Combined sources9
Helixi70 – 88Combined sources19
Helixi92 – 94Combined sources3
Beta strandi95 – 102Combined sources8

3D structure databases

ProteinModelPortaliP30904
SMRiP30904
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30904

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

eggNOGiKOG1759 Eukaryota
ENOG41122MF LUCA
GeneTreeiENSGT00910000144267
HOGENOMiHOG000112325
HOVERGENiHBG003240
InParanoidiP30904
KOiK07253
OMAiPDRIYIN
OrthoDBiEOG091G0ZNJ
PhylomeDBiP30904
TreeFamiTF313853

Family and domain databases

Gene3Di3.30.429.10, 1 hit
InterProiView protein in InterPro
IPR001398 Macrophage_inhib_fac
IPR019829 Macrophage_inhib_fac_CS
IPR014347 Tautomerase/MIF_sf
PANTHERiPTHR11954 PTHR11954, 1 hit
PfamiView protein in Pfam
PF01187 MIF, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004816 Macrophage_inhib_fac, 1 hit
SUPFAMiSSF55331 SSF55331, 1 hit
PROSITEiView protein in PROSITE
PS01158 MIF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF
60 70 80 90 100
SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY
110
DMNAANVGWN GSTFA
Length:115
Mass (Da):12,477
Last modified:January 23, 2007 - v4
Checksum:i8333F7B81B098445
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51S → R in AAA62644 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73424 mRNA Translation: AAB32392.1
U20999 mRNA Translation: AAA62644.1
U62326 mRNA Translation: AAB04024.1
BC061545 mRNA Translation: AAH61545.1
PIRiS27117
RefSeqiNP_112313.1, NM_031051.1
XP_008772068.1, XM_008773846.2
XP_017457271.1, XM_017601782.1
UniGeneiRn.2661

Genome annotation databases

EnsembliENSRNOT00000008608; ENSRNOP00000008608; ENSRNOG00000006589
ENSRNOT00000077564; ENSRNOP00000074316; ENSRNOG00000056076
GeneIDi103694877
81683
KEGGirno:103694877
rno:81683
UCSCiRGD:621163 rat

Similar proteinsi

Entry informationi

Entry nameiMIF_RAT
AccessioniPrimary (citable) accession number: P30904
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 158 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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