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Protein

Sensor protein EvgS

Gene

evgS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system EvgS/EvgA. Phosphorylates EvgA via a four-step phosphorelay in response to environmental signals.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histidine phosphotransfer kinase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
  • response to acidic pH Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EVGS-MONOMER
BRENDAi2.7.13.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein EvgS (EC:2.7.13.3)
Gene namesi
Name:evgS
Ordered Locus Names:b2370, JW2367
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11610 evgS

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 325CytoplasmicSequence analysisAdd BLAST304
Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
Topological domaini347 – 537PeriplasmicSequence analysisAdd BLAST191
Transmembranei538 – 558HelicalSequence analysisAdd BLAST21
Topological domaini559 – 1197CytoplasmicSequence analysisAdd BLAST639

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000003237122 – 1197Sensor protein EvgSAdd BLAST1176

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei721Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1
Modified residuei10094-aspartylphosphatePROSITE-ProRule annotation1
Modified residuei1137PhosphohistidinePROSITE-ProRule annotation1

Post-translational modificationi

Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30855
PRIDEiP30855

PTM databases

iPTMnetiP30855

Interactioni

Protein-protein interaction databases

BioGridi4260559, 16 interactors
DIPiDIP-9545N
IntActiP30855, 5 interactors
STRINGi316407.1799781

Structurei

3D structure databases

ProteinModelPortaliP30855
SMRiP30855
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini718 – 938Histidine kinasePROSITE-ProRule annotationAdd BLAST221
Domaini960 – 1074Response regulatoryPROSITE-ProRule annotationAdd BLAST115
Domaini1098 – 1197HPtPROSITE-ProRule annotationAdd BLAST100

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
COG0784 LUCA
COG0834 LUCA
COG2198 LUCA
HOGENOMiHOG000122879
InParanoidiP30855
KOiK07679
OMAiHWILPYR
PhylomeDBiP30855

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
cd00088 HPT, 1 hit
cd00156 REC, 1 hit
Gene3Di1.20.120.160, 1 hit
3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR036641 HPT_dom_sf
IPR004358 Sig_transdc_His_kin-like_C
IPR008207 Sig_transdc_His_kin_Hpt_dom
IPR001789 Sig_transdc_resp-reg_receiver
IPR001638 Solute-binding_3/MltF_N
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PF01627 Hpt, 1 hit
PF00072 Response_reg, 1 hit
PF00497 SBP_bac_3, 2 hits
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SM00073 HPT, 1 hit
SM00062 PBPb, 2 hits
SM00448 REC, 1 hit
SUPFAMiSSF47226 SSF47226, 1 hit
SSF47384 SSF47384, 1 hit
SSF52172 SSF52172, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit
PS50894 HPT, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLPYIFLL CCGLWSTISF ADEDYIEYRG ISSNNRVTLD PLRLSNKELR
60 70 80 90 100
WLASKKNLVI AVHKSQTATL LHTDSQQRVR GINADYLNLL KRALNIKLTL
110 120 130 140 150
REYADHQKAM DALAEGEVDI VLSHLVTSPP LNNDIAATKP LIITFPALVT
160 170 180 190 200
TLHDSMRPLT SPKPVNIARV ANYPPDEVIH QSFPKATIIS FTNLYQALAS
210 220 230 240 250
VSAGHNDYFI GSNIITSSMI SRYFTHSLNV VKYYNSPRQY NFFLTRKESV
260 270 280 290 300
ILNEVLNRFV DALTNEVRYE VSQNWLDTGN LAFLNKPLEL TEHEKQWIKQ
310 320 330 340 350
HPNLKVLENP YSPPYSMTDE NGSVRGVMGD ILNIITLQTG LNFSPITVSH
360 370 380 390 400
NIHAGTQLSP GGWDIIPGAI YSEDRENNVL FAEAFITTPY VFVMQKAPDS
410 420 430 440 450
EQTLKKGMKV AIPYYYELHS QLKEMYPEVE WIQVDNASAA FHKVKEGELD
460 470 480 490 500
ALVATQLNSR YMIDHYYPNE LYHFLIPGVP NASLSFAFPR GEPELKDIIN
510 520 530 540 550
KALNAIPPSE VLRLTEKWIK MPNVTIDTWD LYSEQFYIVT TLSVLLVGSS
560 570 580 590 600
LLWGFYLLRS VRRRKVIQGD LENQISFRKA LSDSLPNPTY VVNWQGNVIS
610 620 630 640 650
HNSAFEHYFT ADYYKNAMLP LENSDSPFKD VFSNAHEVTA ETKENRTIYT
660 670 680 690 700
QVFEIDNGIE KRCINHWHTL CNLPASDNAV YICGWQDITE TRDLINALEV
710 720 730 740 750
EKNKAIKATV AKSQFLATMS HEIRTPISSI MGFLELLSGS GLSKEQRVEA
760 770 780 790 800
ISLAYATGQS LLGLIGEILD VDKIESGNYQ LQPQWVDIPT LVQNTCHSFG
810 820 830 840 850
AIAASKSIAL SCSSTFPEHY LVKIDPQAFK QVLSNLLSNA LKFTTEGAVK
860 870 880 890 900
ITTSLGHIDD NHAVIKMTIM DSGSGLSQEE QQQLFKRYSQ TSAGRQQTGS
910 920 930 940 950
GLGLMICKEL IKNMQGDLSL ESHPGIGTTF TITIPVEISQ QVATVEAKAE
960 970 980 990 1000
QPITLPEKLS ILIADDHPTN RLLLKRQLNL LGYDVDEATD GVQALHKVSM
1010 1020 1030 1040 1050
QHYDLLITDV NMPNMDGFEL TRKLREQNSS LPIWGLTANA QANEREKGLS
1060 1070 1080 1090 1100
CGMNLCLFKP LTLDVLKTHL SQLHQVAHIA PQYRHLDIEA LKNNTANDLQ
1110 1120 1130 1140 1150
LMQEILMTFQ HETHKDLPAA FQALEAGDNR TFHQCIHRIH GAANILNLQK
1160 1170 1180 1190
LINISHQLEI TPVSDDSKPE ILQLLNSVKE HIAELDQEIA VFCQKND
Length:1,197
Mass (Da):134,743
Last modified:November 1, 1997 - v2
Checksum:iE8E1DE0F797B1278
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152L → F (PubMed:8125343).Curated1
Sequence conflicti152L → F (PubMed:1289796).Curated1
Sequence conflicti242 – 243FF → PL (PubMed:8125343).Curated2
Sequence conflicti242 – 243FF → PL (PubMed:1289796).Curated2
Sequence conflicti275W → R (PubMed:8125343).Curated1
Sequence conflicti275W → R (PubMed:1289796).Curated1
Sequence conflicti420 – 421SQ → FE (PubMed:8125343).Curated2
Sequence conflicti420 – 421SQ → FE (PubMed:1289796).Curated2
Sequence conflicti739G → D (PubMed:8125343).Curated1
Sequence conflicti739G → D (PubMed:1289796).Curated1
Sequence conflicti758G → K (PubMed:8125343).Curated1
Sequence conflicti758G → K (PubMed:1289796).Curated1
Sequence conflicti761L → V (PubMed:8125343).Curated1
Sequence conflicti761L → V (PubMed:1289796).Curated1
Sequence conflicti877S → L (PubMed:8125343).Curated1
Sequence conflicti877S → L (PubMed:1289796).Curated1
Sequence conflicti1045R → H (PubMed:8125343).Curated1
Sequence conflicti1045R → H (PubMed:1289796).Curated1
Sequence conflicti1074H → Y (PubMed:8125343).Curated1
Sequence conflicti1074H → Y (PubMed:1289796).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti577F → S in EvgS1; constitutively active. 1
Natural varianti701E → G in EvgS4; constitutively active. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14008 Genomic DNA Translation: BAA03108.1
AF201840 Genomic DNA Translation: AAF17563.1
AF201841 Genomic DNA Translation: AAF17564.1
U00096 Genomic DNA Translation: AAC75429.1
AP009048 Genomic DNA Translation: BAA16241.1
PIRiG65010
RefSeqiNP_416871.1, NC_000913.3
WP_001326970.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75429; AAC75429; b2370
BAA16241; BAA16241; BAA16241
GeneIDi946844
KEGGiecj:JW2367
eco:b2370
PATRICifig|1411691.4.peg.4359

Similar proteinsi

Entry informationi

Entry nameiEVGS_ECOLI
AccessioniPrimary (citable) accession number: P30855
Secondary accession number(s): P77644, Q9RF36, Q9RF37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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