UniProtKB - P30845 (EPTA_ECOLI)
Protein
Phosphoethanolamine transferase EptA
Gene
eptA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin.By similarity
GO - Molecular functioni
- phosphotransferase activity, phosphate group as acceptor Source: GO_Central
- sulfuric ester hydrolase activity Source: InterPro
GO - Biological processi
- lipid A biosynthetic process Source: UniProtKB-KW
- lipopolysaccharide biosynthetic process Source: EcoCyc
- lipopolysaccharide core region biosynthetic process Source: GO_Central
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Antibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11613-MONOMER MetaCyc:EG11613-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoethanolamine transferase EptA (EC:2.7.-.-)Alternative name(s): Polymyxin resistance protein PmrC |
Gene namesi | Name:eptA Synonyms:pmrC, yjdB Ordered Locus Names:b4114, JW5730 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 10 – 30 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 31 – 47 | PeriplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 48 – 68 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 69 – 79 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 80 – 100 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 101 – 123 | PeriplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 124 – 144 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 145 – 154 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 155 – 175 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 176 – 547 | PeriplasmicSequence analysisAdd BLAST | 372 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209151 | 1 – 547 | Phosphoethanolamine transferase EptAAdd BLAST | 547 |
Proteomic databases
jPOSTi | P30845 |
PaxDbi | P30845 |
PRIDEi | P30845 |
Expressioni
Inductioni
The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions.By similarity
Interactioni
Subunit structurei
Has been isolated as a 91 kDa complex containing ZipA-EptA and an unidentified 24 kDa protein.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263082, 125 interactors |
DIPi | DIP-12561N |
IntActi | P30845, 2 interactors |
STRINGi | 511145.b4114 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2194, Bacteria |
HOGENOMi | CLU_018534_1_0_6 |
InParanoidi | P30845 |
PhylomeDBi | P30845 |
Family and domain databases
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR012549, EptA-like_N IPR040423, PEA_transferase IPR000917, Sulfatase_N |
PANTHERi | PTHR30443, PTHR30443, 1 hit |
Pfami | View protein in Pfam PF08019, EptA_B_N, 1 hit PF00884, Sulfatase, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
i Sequence
Sequence statusi: Complete.
P30845-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKRLLKRPS LNLLAWLLLA AFYISICLNI AFFKQVLQAL PLDSLHNVLV
60 70 80 90 100
FLSMPVVAFS VINIVLTLSS FLWLNRPLAC LFILVGAAAQ YFIMTYGIVI
110 120 130 140 150
DRSMIANIID TTPAESYALM TPQMLLTLGF SGVLAALIAC WIKIKPATSR
160 170 180 190 200
LRSVLFRGAN ILVSVLLILL VAALFYKDYA SLFRNNKELV KSLSPSNSIV
210 220 230 240 250
ASWSWYSHQR LANLPLVRIG EDAHRNPLMQ NEKRKNLTIL IVGETSRAEN
260 270 280 290 300
FSLNGYPRET NPRLAKDNVV YFPNTASCGT ATAVSVPCMF SDMPREHYKE
310 320 330 340 350
ELAQHQEGVL DIIQRAGINV LWNDNDGGCK GACDRVPHQN VTALNLPDQC
360 370 380 390 400
INGECYDEVL FHGLEEYINN LQGDGVIVLH TIGSHGPTYY NRYPPQFRKF
410 420 430 440 450
TPTCDTNEIQ TCTKEQLVNT YDNTLVYVDY IVDKAINLLK EHQDKFTTSL
460 470 480 490 500
VYLSDHGESL GENGIYLHGL PYAIAPDSQK QVPMLLWLSE DYQKRYQVDQ
510 520 530 540
NCLQKQAQTQ HYSQDNLFST LLGLTGVETK YYQAADDILQ TCRRVSE
Sequence cautioni
The sequence AAA97013 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97013.1 Different initiation. U00096 Genomic DNA Translation: AAC77075.2 AP009048 Genomic DNA Translation: BAE78116.1 D14055 Genomic DNA Translation: BAA03142.1 |
RefSeqi | NP_418538.2, NC_000913.3 WP_000919792.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77075; AAC77075; b4114 BAE78116; BAE78116; BAE78116 |
GeneIDi | 948629 |
KEGGi | ecj:JW5730 eco:b4114 |
PATRICi | fig|1411691.4.peg.2586 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97013.1 Different initiation. U00096 Genomic DNA Translation: AAC77075.2 AP009048 Genomic DNA Translation: BAE78116.1 D14055 Genomic DNA Translation: BAA03142.1 |
RefSeqi | NP_418538.2, NC_000913.3 WP_000919792.1, NZ_LN832404.1 |
3D structure databases
SMRi | P30845 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263082, 125 interactors |
DIPi | DIP-12561N |
IntActi | P30845, 2 interactors |
STRINGi | 511145.b4114 |
Proteomic databases
jPOSTi | P30845 |
PaxDbi | P30845 |
PRIDEi | P30845 |
Genome annotation databases
EnsemblBacteriai | AAC77075; AAC77075; b4114 BAE78116; BAE78116; BAE78116 |
GeneIDi | 948629 |
KEGGi | ecj:JW5730 eco:b4114 |
PATRICi | fig|1411691.4.peg.2586 |
Organism-specific databases
EchoBASEi | EB1570 |
Phylogenomic databases
eggNOGi | COG2194, Bacteria |
HOGENOMi | CLU_018534_1_0_6 |
InParanoidi | P30845 |
PhylomeDBi | P30845 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11613-MONOMER MetaCyc:EG11613-MONOMER |
Miscellaneous databases
PROi | PR:P30845 |
Family and domain databases
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR012549, EptA-like_N IPR040423, PEA_transferase IPR000917, Sulfatase_N |
PANTHERi | PTHR30443, PTHR30443, 1 hit |
Pfami | View protein in Pfam PF08019, EptA_B_N, 1 hit PF00884, Sulfatase, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EPTA_ECOLI | |
Accessioni | P30845Primary (citable) accession number: P30845 Secondary accession number(s): P76793, Q2M6J0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families