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Protein

Sensor protein BasS

Gene

basS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system BasS/BasR Autophosphorylates and activates BasR by phosphorylation.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc
  • protein histidine kinase activity Source: EcoCyc

GO - Biological processi

  • peptidyl-histidine phosphorylation Source: EcoCyc
  • response to iron(III) ion Source: EcoCyc
  • signal transduction by protein phosphorylation Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:BASS-MONOMER
BRENDAi2.7.13.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein BasS (EC:2.7.13.3)
Gene namesi
Name:basS
Synonyms:pmrB
Ordered Locus Names:b4112, JW4073
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11614 basS

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Topological domaini35 – 64PeriplasmicSequence analysisAdd BLAST30
Transmembranei65 – 88HelicalSequence analysisAdd BLAST24
Topological domaini89 – 363CytoplasmicSequence analysisAdd BLAST275

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747011 – 363Sensor protein BasSAdd BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30844
PRIDEiP30844

PTM databases

iPTMnetiP30844

Expressioni

Inductioni

The eptA-basRS operon is positively autoregulated by BasR under high iron or aluminum concentration conditions.By similarity

Interactioni

Protein-protein interaction databases

BioGridi4263084, 4 interactors
DIPiDIP-9202N
IntActiP30844, 4 interactors
STRINGi316385.ECDH10B_4304

Structurei

3D structure databases

ProteinModelPortaliP30844
SMRiP30844
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 141HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini149 – 357Histidine kinasePROSITE-ProRule annotationAdd BLAST209

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105ECM Bacteria
ENOG410XPUQ LUCA
HOGENOMiHOG000127051
InParanoidiP30844
KOiK07643
OMAiNAYRYSP
PhylomeDBiP30844

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR003660 HAMP_dom
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR004358 Sig_transdc_His_kin-like_C
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00304 HAMP, 1 hit
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SUPFAMiSSF47384 SSF47384, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS50885 HAMP, 1 hit
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

P30844-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFLRRPISL RQRLILTIGA ILLVFELISV FWLWHESTEQ IQLFEQALRD
60 70 80 90 100
NRNNDRHIMR EIREAVASLI VPGVFMVSLT LFICYQAVRR ITRPLAELQK
110 120 130 140 150
ELEARTADNL TPIAIHSATL EIEAVVSALN DLVSRLTSTL DNERLFTADV
160 170 180 190 200
AHELRTPLAG VRLHLELLAK THHIDVAPLV ARLDQMMESV SQLLQLARAG
210 220 230 240 250
QSFSSGNYQH VKLLEDVILP SYDELSTMLD QRQQTLLLPE SAADITVQGD
260 270 280 290 300
ATLLRMLLRN LVENAHRYSP QGSNIMIKLQ EDDGAVMAVE DEGPGIDESK
310 320 330 340 350
CGELSKAFVR MDSRYGGIGL GLSIVSRITQ LHHGQFFLQN RQETSGTRAW
360
VRLKKDQYVA NQI
Length:363
Mass (Da):41,029
Last modified:July 1, 1993 - v1
Checksum:iA390AAB9EB425750
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14055 Genomic DNA Translation: BAA03144.1
U14003 Genomic DNA Translation: AAA97011.1
U00096 Genomic DNA Translation: AAC77073.1
AP009048 Genomic DNA Translation: BAE78114.1
PIRiJX0285
RefSeqiNP_418536.1, NC_000913.3
WP_001300761.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77073; AAC77073; b4112
BAE78114; BAE78114; BAE78114
GeneIDi948632
KEGGiecj:JW4073
eco:b4112
PATRICifig|83333.103.peg.419

Similar proteinsi

Entry informationi

Entry nameiBASS_ECOLI
AccessioniPrimary (citable) accession number: P30844
Secondary accession number(s): Q2M6J2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 18, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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