UniProtKB - P30776 (RAD21_SCHPO)
Cohesin subunit rad21
rad21
Functioni
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by cut1 and dissociates from chromatin, allowing sister chromatids to segregate. Also involved in the DNA double-strand-break (DSB) repair system.
2 PublicationsGO - Molecular functioni
- chromatin binding Source: GO_Central
GO - Biological processi
- cell division Source: UniProtKB-KW
- cellular response to DNA damage stimulus Source: PomBase
- double-strand break repair Source: PomBase
- mitotic cohesin loading Source: PomBase
- mitotic cohesin ssDNA (lagging strand) loading Source: PomBase
- mitotic sister chromatid cohesion Source: PomBase
- mitotic sister chromatid segregation Source: PomBase
- mitotic telomere tethering at nuclear periphery Source: PomBase
- replication-born double-strand break repair via sister chromatid exchange Source: GO_Central
Keywordsi
Biological process | Cell cycle, Cell division, Chromosome partition, DNA damage, DNA repair, Mitosis |
Enzyme and pathway databases
Reactomei | R-SPO-2470946, Cohesin Loading onto Chromatin R-SPO-2500257, Resolution of Sister Chromatid Cohesion R-SPO-3108214, SUMOylation of DNA damage response and repair proteins |
Names & Taxonomyi
Protein namesi | Recommended name: Cohesin subunit rad21Alternative name(s): Double-strand-break repair protein rad21 SCC1 homolog |
Gene namesi | Name:rad21 ORF Names:SPCC338.17c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPCC338.17c, rad21 |
VEuPathDBi | FungiDB:SPCC338.17c |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- centromere 1 Publication
Note: Associates with chromatin. Cohesin complex mainly associates with broad centromere region. Also associates with mating-type heterochromatic region.
Nucleus
- nuclear meiotic cohesin complex Source: GO_Central
- nuclear mitotic cohesin complex Source: PomBase
- nucleus Source: PomBase
- nucleus leading edge Source: PomBase
Other locations
- chromatin Source: PomBase
- chromosome, centromeric outer repeat region Source: PomBase
- chromosome, subtelomeric region Source: PomBase
- condensed chromosome, centromeric region Source: PomBase
- heterochromatin island Source: PomBase
- mitotic cohesin complex Source: PomBase
- pericentric heterochromatin Source: PomBase
- rDNA heterochromatin Source: PomBase
Keywords - Cellular componenti
Centromere, Chromosome, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 179 | R → E: Sister chromatid separation blocked; when associated with E-231. 1 Publication | 1 | |
Mutagenesisi | 231 | R → E: Sister chromatid separation blocked; when associated with E-179. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097871 | 1 – 628 | Cohesin subunit rad21Add BLAST | 628 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 219 | Phosphoserine1 Publication | 1 | |
Modified residuei | 547 | Phosphoserine1 Publication | 1 | |
Modified residuei | 553 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 179 – 180 | CleavageCurated | 2 | |
Sitei | 231 – 232 | CleavageCurated | 2 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P30776 |
PaxDbi | P30776 |
PTM databases
iPTMneti | P30776 |
Interactioni
Subunit structurei
Cohesin complexes are composed of the psm1/smc1 and psm3/smc3 heterodimer attached via their hinge domain, rad21/scc1 which link them, and psc3/scc3, which interacts with rad21.
Interacts with ssl3.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 276010, 34 interactors |
ELMi | P30776 |
IntActi | P30776, 3 interactors |
STRINGi | 4896.SPCC338.17c.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 439 – 466 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 439 – 460 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1213, Eukaryota |
HOGENOMi | CLU_015775_0_0_1 |
InParanoidi | P30776 |
OMAi | PLCLNTN |
PhylomeDBi | P30776 |
Family and domain databases
Gene3Di | 1.10.10.580, 1 hit |
InterProi | View protein in InterPro IPR039781, Rad21/Rec8-like IPR006909, Rad21/Rec8_C_eu IPR006910, Rad21_Rec8_N IPR023093, ScpA-like_C IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12585, PTHR12585, 1 hit |
Pfami | View protein in Pfam PF04824, Rad21_Rec8, 1 hit PF04825, Rad21_Rec8_N, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MFYSEAILSK KGPLAKVWLA AHWEKKLSKV QTLHTSIEQS VHAIVTEETA
60 70 80 90 100
PMALRLSGQL MLGVVRIYSR KARYLLEDCT EALMRLKMSF QPGQVDMIEP
110 120 130 140 150
ATALQSLKGK DAVTQSANLT LPETITEFDL LVPDSTFDFQ WSQLLRTPSR
160 170 180 190 200
SSNTLELHSL PISSSPSFPS SQLSIEAGRN AQVESGFSLG ESFAHVGNDM
210 220 230 240 250
QFHLPISNSG AATPRSVHSD NQSQISIEVG RDAPAAAATD LSGIIGPQMT
260 270 280 290 300
KSPASSVTHF STPSMLPIGG TSLDDELLAP VDDLNLDLGL DDLLGDEQGA
310 320 330 340 350
NAPAIEADEQ AETSSIHLPS DIMEDDSSRP AAAGVEEGQV VESATAPQQE
360 370 380 390 400
KINPQKTVRR QRAIIDPVTE LSSKQMKKQL ADTSSITSPL CLNTSSIVFN
410 420 430 440 450
ATVNFTRNGK FNTSIFSSNL NPKVNELLQA DFKQAILRKR KNESPEEVEP
460 470 480 490 500
AKHQRTDTST ENQETAEVLD PEEIAAAELA NITEAAIATL PQETVVQPEG
510 520 530 540 550
EAPELGSPMG FPVTALESAD DSLFDAPPVM LDEADLLGSE RLDSSVSEAL
560 570 580 590 600
PSSQTAKDSL RNKWDPYTEG EKVSFQTLSA GCNREEAVQL FFDVLVLATK
610 620
DVISVKQDVA IQNEITLTAK RGMLLSSL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96437 Genomic DNA Translation: AAA35330.1 CU329672 Genomic DNA Translation: CAA19348.1 |
PIRi | S35547 |
RefSeqi | NP_588151.1, NM_001023140.2 |
Genome annotation databases
EnsemblFungii | SPCC338.17c.1; SPCC338.17c.1:pep; SPCC338.17c |
GeneIDi | 2539447 |
KEGGi | spo:SPCC338.17c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96437 Genomic DNA Translation: AAA35330.1 CU329672 Genomic DNA Translation: CAA19348.1 |
PIRi | S35547 |
RefSeqi | NP_588151.1, NM_001023140.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6YUF | electron microscopy | 3.94 | B | 1-628 | [»] | |
SMRi | P30776 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 276010, 34 interactors |
ELMi | P30776 |
IntActi | P30776, 3 interactors |
STRINGi | 4896.SPCC338.17c.1 |
PTM databases
iPTMneti | P30776 |
Proteomic databases
MaxQBi | P30776 |
PaxDbi | P30776 |
Genome annotation databases
EnsemblFungii | SPCC338.17c.1; SPCC338.17c.1:pep; SPCC338.17c |
GeneIDi | 2539447 |
KEGGi | spo:SPCC338.17c |
Organism-specific databases
PomBasei | SPCC338.17c, rad21 |
VEuPathDBi | FungiDB:SPCC338.17c |
Phylogenomic databases
eggNOGi | KOG1213, Eukaryota |
HOGENOMi | CLU_015775_0_0_1 |
InParanoidi | P30776 |
OMAi | PLCLNTN |
PhylomeDBi | P30776 |
Enzyme and pathway databases
Reactomei | R-SPO-2470946, Cohesin Loading onto Chromatin R-SPO-2500257, Resolution of Sister Chromatid Cohesion R-SPO-3108214, SUMOylation of DNA damage response and repair proteins |
Miscellaneous databases
PROi | PR:P30776 |
Family and domain databases
Gene3Di | 1.10.10.580, 1 hit |
InterProi | View protein in InterPro IPR039781, Rad21/Rec8-like IPR006909, Rad21/Rec8_C_eu IPR006910, Rad21_Rec8_N IPR023093, ScpA-like_C IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR12585, PTHR12585, 1 hit |
Pfami | View protein in Pfam PF04824, Rad21_Rec8, 1 hit PF04825, Rad21_Rec8_N, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD21_SCHPO | |
Accessioni | P30776Primary (citable) accession number: P30776 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families