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Protein

Methionine import ATP-binding protein MetN

Gene

metN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system (Probable). It has also been shown to be involved in formyl-L-methionine transport.UniRule annotationCurated2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • D-methionine transport Source: CACAO

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ABC-MONOMER
MetaCyc:ABC-MONOMER

Protein family/group databases

TCDBi3.A.1.24.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine import ATP-binding protein MetNUniRule annotation (EC:3.6.3.-UniRule annotation)
Gene namesi
Name:metNUniRule annotation
Synonyms:abc
Ordered Locus Names:b0199, JW0195
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11621 metN

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000925031 – 343Methionine import ATP-binding protein MetNAdd BLAST343

Proteomic databases

PaxDbiP30750
PRIDEiP30750

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetI) and a solute-binding protein (MetQ).UniRule annotation

Binary interactionsi

Protein-protein interaction databases

BioGridi4259755, 38 interactors
ComplexPortaliCPX-2114 ATP-dependent methionine-importing complex
DIPiDIP-9027N
IntActiP30750, 57 interactors
STRINGi316385.ECDH10B_0180

Structurei

Secondary structure

1343
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP30750
SMRiP30750
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30750

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 241ABC transporterUniRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Methionine importer (TC 3.A.1.24) family. [View classification]UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JEI Bacteria
COG1135 LUCA
InParanoidiP30750
KOiK02071
PhylomeDBiP30750

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR026253 ABC_MetN
IPR012692 ABC_MetN_proteobac
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR017908 ABC_transprt_methionine_MetN_C
IPR018449 NIL_domain
IPR027417 P-loop_NTPase
PANTHERiPTHR43166:SF5 PTHR43166:SF5, 1 hit
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
PF09383 NIL, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00930 NIL, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02314 ABC_MetN, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
PS51264 METN, 1 hit

Sequencei

Sequence statusi: Complete.

P30750-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIKLSNITKV FHQGTRTIQA LNNVSLHVPA GQIYGVIGAS GAGKSTLIRC
60 70 80 90 100
VNLLERPTEG SVLVDGQELT TLSESELTKA RRQIGMIFQH FNLLSSRTVF
110 120 130 140 150
GNVALPLELD NTPKDEVKRR VTELLSLVGL GDKHDSYPSN LSGGQKQRVA
160 170 180 190 200
IARALASNPK VLLCDEATSA LDPATTRSIL ELLKDINRRL GLTILLITHE
210 220 230 240 250
MDVVKRICDC VAVISNGELI EQDTVSEVFS HPKTPLAQKF IQSTLHLDIP
260 270 280 290 300
EDYQERLQAE PFTDCVPMLR LEFTGQSVDA PLLSETARRF NVNNNIISAQ
310 320 330 340
MDYAGGVKFG IMLTEMHGTQ QDTQAAIAWL QEHHVKVEVL GYV
Length:343
Mass (Da):37,788
Last modified:November 1, 1997 - v4
Checksum:i2FC796C605216FB3
GO

Sequence cautioni

The sequence AAC36869 differs from that shown. Reason: Frameshift at position 200.Curated
The sequence BAA03659 differs from that shown. Reason: Frameshift at positions 106 and 156.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165D → V in AAC36869 (PubMed:7904973).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15061 Genomic DNA Translation: BAA03659.1 Frameshift.
U70214 Genomic DNA Translation: AAB08627.1
U00096 Genomic DNA Translation: AAC73310.1
AP009048 Genomic DNA Translation: BAA77876.2
L08626 Unassigned DNA Translation: AAC36869.1 Frameshift.
PIRiG64744
RefSeqiNP_414741.1, NC_000913.3
WP_000594006.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73310; AAC73310; b0199
BAA77876; BAA77876; BAA77876
GeneIDi944896
KEGGiecj:JW0195
eco:b0199
PATRICifig|1411691.4.peg.2079

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15061 Genomic DNA Translation: BAA03659.1 Frameshift.
U70214 Genomic DNA Translation: AAB08627.1
U00096 Genomic DNA Translation: AAC73310.1
AP009048 Genomic DNA Translation: BAA77876.2
L08626 Unassigned DNA Translation: AAC36869.1 Frameshift.
PIRiG64744
RefSeqiNP_414741.1, NC_000913.3
WP_000594006.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DHWX-ray3.70C/D/G/H1-343[»]
3DHXX-ray2.10A/B246-343[»]
3TUIX-ray2.90C/D/G/H1-343[»]
3TUJX-ray4.00C/D1-343[»]
3TUZX-ray3.40C/D/G/H1-343[»]
ProteinModelPortaliP30750
SMRiP30750
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259755, 38 interactors
ComplexPortaliCPX-2114 ATP-dependent methionine-importing complex
DIPiDIP-9027N
IntActiP30750, 57 interactors
STRINGi316385.ECDH10B_0180

Protein family/group databases

TCDBi3.A.1.24.1 the atp-binding cassette (abc) superfamily

Proteomic databases

PaxDbiP30750
PRIDEiP30750

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73310; AAC73310; b0199
BAA77876; BAA77876; BAA77876
GeneIDi944896
KEGGiecj:JW0195
eco:b0199
PATRICifig|1411691.4.peg.2079

Organism-specific databases

EchoBASEiEB1578
EcoGeneiEG11621 metN

Phylogenomic databases

eggNOGiENOG4108JEI Bacteria
COG1135 LUCA
InParanoidiP30750
KOiK02071
PhylomeDBiP30750

Enzyme and pathway databases

BioCyciEcoCyc:ABC-MONOMER
MetaCyc:ABC-MONOMER

Miscellaneous databases

EvolutionaryTraceiP30750
PROiPR:P30750

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR026253 ABC_MetN
IPR012692 ABC_MetN_proteobac
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR017908 ABC_transprt_methionine_MetN_C
IPR018449 NIL_domain
IPR027417 P-loop_NTPase
PANTHERiPTHR43166:SF5 PTHR43166:SF5, 1 hit
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
PF09383 NIL, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00930 NIL, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02314 ABC_MetN, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
PS51264 METN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMETN_ECOLI
AccessioniPrimary (citable) accession number: P30750
Secondary accession number(s): P77517, Q47615
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: November 7, 2018
This is version 157 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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