Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTP 3',8-cyclase

Gene

moaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).
Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.UniRule annotation

Catalytic activityi

GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17GTPUniRule annotation1
Metal bindingi24Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi28Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei30S-adenosyl-L-methionineUniRule annotation1
Metal bindingi31Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei68GTPUniRule annotation1
Binding sitei72S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei99GTPUniRule annotation1
Binding sitei123S-adenosyl-L-methionineUniRule annotation1
Binding sitei160GTPUniRule annotation1
Binding sitei194S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi257Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi260Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi274Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi262 – 264GTPUniRule annotation3

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
  • GTP 3',8'-cyclase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-UniRule
  • metal ion binding Source: UniProtKB-KW
  • S-adenosyl-L-methionine binding Source: UniProtKB-UniRule

GO - Biological processi

  • Mo-molybdopterin cofactor biosynthetic process Source: EcoCyc
  • response to heat Source: EcoCyc

Keywordsi

Molecular functionLyase
Biological processMolybdenum cofactor biosynthesis
Ligand4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11595-MONOMER
MetaCyc:EG11595-MONOMER
UniPathwayi
UPA00344

Names & Taxonomyi

Protein namesi
Recommended name:
GTP 3',8-cyclaseUniRule annotation (EC:4.1.99.22UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Synonyms:bisA, chlA, chlA1, narA
Ordered Locus Names:b0781, JW0764
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11595 moaA

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001529581 – 329GTP 3',8-cyclaseAdd BLAST329

Proteomic databases

PaxDbiP30745
PRIDEiP30745

Expressioni

Inductioni

By anaerobiosis, repressed by the molybdenum cofactor.

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Protein-protein interaction databases

BioGridi4259951, 33 interactors
DIPiDIP-10228N
IntActiP30745, 14 interactors
STRINGi316385.ECDH10B_0849

Structurei

3D structure databases

ProteinModelPortaliP30745
SMRiP30745
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CM1 Bacteria
COG2896 LUCA
HOGENOMiHOG000228682
InParanoidiP30745
KOiK03639
PhylomeDBiP30745

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01225_B MoaA_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR034481 Main_SPASM_domain-containing
IPR013483 MoaA
IPR040064 MoaA-like
IPR000385 MoaA_NifB_PqqE_Fe-S-bd_CS
IPR010505 Mob_synth_C
IPR007197 rSAM
IPR034395 SPASM/twitch_domain-containing
PfamiView protein in Pfam
PF06463 Mob_synth_C, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01067 SPASM/twitch_domain_containing, 1 hit
SFLDG01383 cyclic_pyranopterin_phosphate_, 1 hit
SFLDG01386 main_SPASM_domain-containing, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR02666 moaA, 1 hit
PROSITEiView protein in PROSITE
PS01305 MOAA_NIFB_PQQE, 1 hit

Sequencei

Sequence statusi: Complete.

P30745-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASQLTDAFA RKFYYLRLSI TDVCNFRCTY CLPDGYKPSG VTNKGFLTVD
60 70 80 90 100
EIRRVTRAFA RLGTEKVRLT GGEPSLRRDF TDIIAAVREN DAIRQIAVTT
110 120 130 140 150
NGYRLERDVA SWRDAGLTGI NVSVDSLDAR QFHAITGQDK FNQVMAGIDA
160 170 180 190 200
AFEAGFEKVK VNTVLMRDVN HHQLDTFLNW IQHRPIQLRF IELMETGEGS
210 220 230 240 250
ELFRKHHISG QVLRDELLRR GWIHQLRQRS DGPAQVFCHP DYAGEIGLIM
260 270 280 290 300
PYEKDFCATC NRLRVSSIGK LHLCLFGEGG VNLRDLLEDD TQQQALEARI
310 320
SAALREKKQT HFLHQNNTGI TQNLSYIGG
Length:329
Mass (Da):37,346
Last modified:April 1, 1993 - v1
Checksum:iFE91263C53F33F78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70420 Genomic DNA Translation: CAA49861.1
U00096 Genomic DNA Translation: AAC73868.1
AP009048 Genomic DNA Translation: BAA35439.1
PIRiS31879
RefSeqiNP_415302.1, NC_000913.3
WP_001350494.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73868; AAC73868; b0781
BAA35439; BAA35439; BAA35439
GeneIDi945392
KEGGiecj:JW0764
eco:b0781
PATRICifig|1411691.4.peg.1497

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70420 Genomic DNA Translation: CAA49861.1
U00096 Genomic DNA Translation: AAC73868.1
AP009048 Genomic DNA Translation: BAA35439.1
PIRiS31879
RefSeqiNP_415302.1, NC_000913.3
WP_001350494.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP30745
SMRiP30745
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259951, 33 interactors
DIPiDIP-10228N
IntActiP30745, 14 interactors
STRINGi316385.ECDH10B_0849

Proteomic databases

PaxDbiP30745
PRIDEiP30745

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73868; AAC73868; b0781
BAA35439; BAA35439; BAA35439
GeneIDi945392
KEGGiecj:JW0764
eco:b0781
PATRICifig|1411691.4.peg.1497

Organism-specific databases

EchoBASEiEB1552
EcoGeneiEG11595 moaA

Phylogenomic databases

eggNOGiENOG4105CM1 Bacteria
COG2896 LUCA
HOGENOMiHOG000228682
InParanoidiP30745
KOiK03639
PhylomeDBiP30745

Enzyme and pathway databases

UniPathwayi
UPA00344

BioCyciEcoCyc:EG11595-MONOMER
MetaCyc:EG11595-MONOMER

Miscellaneous databases

PROiPR:P30745

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01225_B MoaA_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR034481 Main_SPASM_domain-containing
IPR013483 MoaA
IPR040064 MoaA-like
IPR000385 MoaA_NifB_PqqE_Fe-S-bd_CS
IPR010505 Mob_synth_C
IPR007197 rSAM
IPR034395 SPASM/twitch_domain-containing
PfamiView protein in Pfam
PF06463 Mob_synth_C, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01067 SPASM/twitch_domain_containing, 1 hit
SFLDG01383 cyclic_pyranopterin_phosphate_, 1 hit
SFLDG01386 main_SPASM_domain-containing, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR02666 moaA, 1 hit
PROSITEiView protein in PROSITE
PS01305 MOAA_NIFB_PQQE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMOAA_ECOLI
AccessioniPrimary (citable) accession number: P30745
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 7, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again