UniProtKB - P30744 (SDHM_ECOLI)
Protein
L-serine dehydratase 2
Gene
sdaB
Organism
Escherichia coli (strain K12)
Status
Functioni
Deaminates also threonine, particularly when it is present in high concentration.
Catalytic activityi
- EC:4.3.1.17
Cofactori
[4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated
: gluconeogenesis Pathwayi
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- L-serine ammonia-lyase activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- gluconeogenesis Source: UniProtKB-UniPathway
- L-serine catabolic process Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Biological process | Gluconeogenesis |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:LSERINEDEAM2-MONOMER ECOL316407:JW2768-MONOMER MetaCyc:LSERINEDEAM2-MONOMER |
BRENDAi | 4.3.1.17 2026 |
UniPathwayi | UPA00138 |
Names & Taxonomyi
Protein namesi | Recommended name: L-serine dehydratase 2 (EC:4.3.1.17)Short name: SDH 2 Alternative name(s): L-serine deaminase 2 Short name: L-SD2 |
Gene namesi | Name:sdaB Ordered Locus Names:b2797, JW2768 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171904 | 1 – 455 | L-serine dehydratase 2Add BLAST | 455 |
Post-translational modificationi
Activated by post-translational modification by a system involving at least three gene products. Activation is mimicked in vitro by iron and dithiothreitol. There is considerable evidence for a free-radical activation mechanism.
Proteomic databases
jPOSTi | P30744 |
PaxDbi | P30744 |
PRIDEi | P30744 |
Expressioni
Inductioni
Transcribed in rich medium, particularly in the absence of glucose, and is under the control of catabolite activator protein. It is made aerobically and anaerobically. Repressed by LeuO. Part of the sdaCB operon.1 Publication
Interactioni
Protein-protein interaction databases
BioGridi | 4261306, 9 interactors |
IntActi | P30744, 2 interactors |
STRINGi | 511145.b2797 |
Family & Domainsi
Sequence similaritiesi
Belongs to the iron-sulfur dependent L-serine dehydratase family.Curated
Phylogenomic databases
eggNOGi | ENOG4105EJQ Bacteria COG1760 LUCA |
HOGENOMi | HOG000036732 |
InParanoidi | P30744 |
KOi | K01752 |
PhylomeDBi | P30744 |
Family and domain databases
Gene3Di | 3.30.1330.90, 1 hit |
InterProi | View protein in InterPro IPR029009 ASB_dom_sf IPR004644 Fe-S_L-Ser_mono IPR005130 Ser_deHydtase-like_asu IPR005131 Ser_deHydtase_bsu |
Pfami | View protein in Pfam PF03313 SDH_alpha, 1 hit PF03315 SDH_beta, 1 hit |
SUPFAMi | SSF143548 SSF143548, 1 hit |
TIGRFAMsi | TIGR00720 sda_mono, 1 hit |
i Sequence
Sequence statusi: Complete.
P30744-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MISVFDIFKI GIGPSSSHTV GPMKAGKQFT DDLIARNLLK DVTRVVVDVY
60 70 80 90 100
GSLSLTGKGH HTDIAIIMGL AGNLPDTVDI DSIPSFIQDV NTHGRLMLAN
110 120 130 140 150
GQHEVEFPVD QCMNFHADNL SLHENGMRIT ALAGDKVVYS QTYYSIGGGF
160 170 180 190 200
IVDEEHFGQQ DSAPVEVPYP YSSAADLQKH CQETGLSLSG LMMKNELALH
210 220 230 240 250
SKEELEQHLA NVWEVMRGGI ERGISTEGVL PGKLRVPRRA AALRRMLVSQ
260 270 280 290 300
DKTTTDPMAV VDWINMFALA VNEENAAGGR VVTAPTNGAC GIIPAVLAYY
310 320 330 340 350
DKFIREVNAN SLARYLLVAS AIGSLYKMNA SISGAEVGCQ GEVGVACSMA
360 370 380 390 400
AAGLAELLGA SPAQVCIAAE IAMEHNLGLT CDPVAGQVQV PCIERNAIAA
410 420 430 440 450
VKAVNAARMA LRRTSEPRVC LDKVIETMYE TGKDMNAKYR ETSRGGLAMK
IVACD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 132 | L → V (PubMed:8385012).Curated | 1 | |
Sequence conflicti | 134 | G → A (PubMed:8385012).Curated | 1 | |
Sequence conflicti | 196 – 197 | EL → DV (PubMed:8385012).Curated | 2 | |
Sequence conflicti | 236 – 239 | VPRR → STPC (PubMed:8385012).Curated | 4 | |
Sequence conflicti | 360 – 362 | ASP → GNR (PubMed:8385012).Curated | 3 | |
Sequence conflicti | 372 | A → G (PubMed:8385012).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07763 Genomic DNA No translation available. U29581 Genomic DNA Translation: AAB40447.1 U00096 Genomic DNA Translation: AAC75839.1 AP009048 Genomic DNA Translation: BAE76869.1 |
PIRi | A65062 |
RefSeqi | NP_417277.1, NC_000913.3 WP_000626422.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75839; AAC75839; b2797 BAE76869; BAE76869; BAE76869 |
GeneIDi | 947262 |
KEGGi | ecj:JW2768 eco:b2797 |
PATRICi | fig|1411691.4.peg.3936 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07763 Genomic DNA No translation available. U29581 Genomic DNA Translation: AAB40447.1 U00096 Genomic DNA Translation: AAC75839.1 AP009048 Genomic DNA Translation: BAE76869.1 |
PIRi | A65062 |
RefSeqi | NP_417277.1, NC_000913.3 WP_000626422.1, NZ_LN832404.1 |
3D structure databases
SMRi | P30744 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 4261306, 9 interactors |
IntActi | P30744, 2 interactors |
STRINGi | 511145.b2797 |
Proteomic databases
jPOSTi | P30744 |
PaxDbi | P30744 |
PRIDEi | P30744 |
Genome annotation databases
EnsemblBacteriai | AAC75839; AAC75839; b2797 BAE76869; BAE76869; BAE76869 |
GeneIDi | 947262 |
KEGGi | ecj:JW2768 eco:b2797 |
PATRICi | fig|1411691.4.peg.3936 |
Organism-specific databases
EchoBASEi | EB1580 |
Phylogenomic databases
eggNOGi | ENOG4105EJQ Bacteria COG1760 LUCA |
HOGENOMi | HOG000036732 |
InParanoidi | P30744 |
KOi | K01752 |
PhylomeDBi | P30744 |
Enzyme and pathway databases
UniPathwayi | UPA00138 |
BioCyci | EcoCyc:LSERINEDEAM2-MONOMER ECOL316407:JW2768-MONOMER MetaCyc:LSERINEDEAM2-MONOMER |
BRENDAi | 4.3.1.17 2026 |
Miscellaneous databases
PROi | PR:P30744 |
Family and domain databases
Gene3Di | 3.30.1330.90, 1 hit |
InterProi | View protein in InterPro IPR029009 ASB_dom_sf IPR004644 Fe-S_L-Ser_mono IPR005130 Ser_deHydtase-like_asu IPR005131 Ser_deHydtase_bsu |
Pfami | View protein in Pfam PF03313 SDH_alpha, 1 hit PF03315 SDH_beta, 1 hit |
SUPFAMi | SSF143548 SSF143548, 1 hit |
TIGRFAMsi | TIGR00720 sda_mono, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SDHM_ECOLI | |
Accessioni | P30744Primary (citable) accession number: P30744 Secondary accession number(s): Q2MA37, Q59377 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | November 1, 1997 | |
Last modified: | November 13, 2019 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene - PATHWAY comments
Index of metabolic and biosynthesis pathways