Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

High mobility group protein B2

Gene

Hmgb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity (By similarity). Involved in V(D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (PubMed:9184213). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (PubMed:19890330). In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE (By similarity). Has antimicrobial activity in gastrointestinal epithelial tissues (By similarity). Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity (PubMed:25306442). May play a role in germ cell differentiation (PubMed:11262228). Involved in modulation of neurogenesis probably by regulation of neural stem proliferation (PubMed:24391977). Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway (PubMed:19805379).By similarity1 Publication6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi9 – 79HMG box 1PROSITE-ProRule annotationAdd BLAST71
DNA bindingi95 – 163HMG box 2PROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processChemotaxis, DNA recombination, Immunity, Inflammatory response, Innate immunity, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-211227 Activation of DNA fragmentation factor

Names & Taxonomyi

Protein namesi
Recommended name:
High mobility group protein B2
Alternative name(s):
High mobility group protein 2
Short name:
HMG-2
Gene namesi
Name:Hmgb2
Synonyms:Hmg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:96157 Hmgb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus, Secreted

Pathology & Biotechi

Disruption phenotypei

Viable, with severe reduction of sperm production in males.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000485351 – 210High mobility group protein B2Add BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3N6-acetyllysineBy similarity1
Disulfide bondi23 ↔ 45In disulfide HMGB2; alternateBy similarity
Modified residuei23Cysteine sulfonic acid (-SO3H); alternateBy similarity1
Modified residuei30N6-acetyllysineCombined sources1
Modified residuei35PhosphoserineBy similarity1
Modified residuei43N6-acetyllysineBy similarity1
Modified residuei45Cysteine sulfonic acid (-SO3H); alternateBy similarity1
Modified residuei90N6-acetyllysineBy similarity1
Modified residuei100PhosphoserineBy similarity1
Modified residuei106Cysteine sulfonic acid (-SO3H)By similarity1
Modified residuei114N6-acetyllysineCombined sources1
Modified residuei141N6-acetyllysineBy similarity1

Post-translational modificationi

Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB2 (HMGB2C23hC45hC106h), 2- disulfide HMGB2 (HMGB2C23-C45C106h) and 3- sulfonyl HMGB2 (HMGB2C23soC45soC106so).By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Oxidation, Phosphoprotein

Proteomic databases

EPDiP30681
MaxQBiP30681
PaxDbiP30681
PeptideAtlasiP30681
PRIDEiP30681

PTM databases

iPTMnetiP30681
PhosphoSitePlusiP30681
SwissPalmiP30681

Expressioni

Tissue specificityi

Widely expressed in embryo. In adult mainly expressed in lymphoid organs and testes (PubMed:11262228). Expressed in primary spermatocytes. Expressed in the superficial zone of articular cartilage (PubMed:19805379).2 Publications

Gene expression databases

BgeeiENSMUSG00000054717 Expressed in 181 organ(s), highest expression level in thymus
CleanExiMM_HMGB2
ExpressionAtlasiP30681 baseline and differential
GenevisibleiP30681 MM

Interactioni

Subunit structurei

Interacts with POU2F2, POU2F1 and POU3F1 (PubMed:7720710). Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2 (PubMed:9184213). Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Directly interacts with SET (By similarity). Interacts with LEF1 (PubMed:19805379).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Lef1P277822EBI-6910056,EBI-984464

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220633, 3 interactors
DIPiDIP-899N
IntActiP30681, 7 interactors
MINTiP30681
STRINGi10090.ENSMUSP00000065940

Structurei

3D structure databases

ProteinModelPortaliP30681
SMRiP30681
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni165 – 180Required for chemotactic activityBy similarityAdd BLAST16

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi186 – 210Asp/Glu-rich (acidic)Add BLAST25

Domaini

Both, HMG box 1 and HMG box 2, show antimicrobial activity.By similarity

Sequence similaritiesi

Belongs to the HMGB family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0381 Eukaryota
COG5648 LUCA
GeneTreeiENSGT00760000119164
HOGENOMiHOG000197861
HOVERGENiHBG009000
InParanoidiP30681
KOiK11295
OMAiADDTWRA
OrthoDBiEOG091G0P81
PhylomeDBiP30681
TreeFamiTF105371

Family and domain databases

Gene3Di1.10.30.10, 2 hits
InterProiView protein in InterPro
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR017967 HMG_boxA_CS
IPR031075 HMGB2
PANTHERiPTHR13711:SF181 PTHR13711:SF181, 1 hit
PfamiView protein in Pfam
PF00505 HMG_box, 1 hit
PF09011 HMG_box_2, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 2 hits
SUPFAMiSSF47095 SSF47095, 2 hits
PROSITEiView protein in PROSITE
PS00353 HMG_BOX_1, 1 hit
PS50118 HMG_BOX_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

P30681-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKGDPNKPR GKMSSYAFFV QTCREEHKKK HPDSSVNFAE FSKKCSERWK
60 70 80 90 100
TMSAKEKSKF EDLAKSDKAR YDREMKNYVP PKGDKKGKKK DPNAPKRPPS
110 120 130 140 150
AFFLFCSENR PKIKIEHPGL SIGDTAKKLG EMWSEQSAKD KQPYEQKAAK
160 170 180 190 200
LKEKYEKDIA AYRAKGKSEA GKKGPGRPTG SKKKNEPEDE EEEEEEEEEE
210
DDEEEEEDEE
Length:210
Mass (Da):24,162
Last modified:January 23, 2007 - v3
Checksum:i45D1F667DB4ED94D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1B0GQX9A0A1B0GQX9_MOUSE
High mobility group protein B2
Hmgb2
99Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7N → I in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti10R → L in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti33D → N in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti41F → I in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti47E → K in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti57K → N in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti70 – 72RYD → CYY in CAA47900 (PubMed:1408807).Curated3
Sequence conflicti80P → S in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti103F → C in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti117H → Y in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti140D → E in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti159I → F in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti164A → V in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti179T → A in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti184 – 193KNEPEDEEEE → NDSED in CAA47900 (PubMed:1408807).Curated10
Sequence conflicti202D → E in CAA47900 (PubMed:1408807).Curated1
Sequence conflicti202D → E in AAG36939 (PubMed:11262228).Curated1
Sequence conflicti202D → E in AAH02050 (PubMed:15489334).Curated1
Sequence conflicti204E → G in CAA47900 (PubMed:1408807).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67668 mRNA Translation: CAA47900.1
Z46757 mRNA Translation: CAA86727.1
AF267733 Genomic DNA Translation: AAG36939.1
AK003773 mRNA Translation: BAB22988.1
AK008443 mRNA Translation: BAB25672.1
AK012568 mRNA Translation: BAB28323.1
AK135296 mRNA Translation: BAE22481.1
AK135297 mRNA Translation: BAE22482.1
AK146212 mRNA Translation: BAE26982.1
BC002050 mRNA Translation: AAH02050.1
BC046759 mRNA Translation: AAH46759.1
BC083108 mRNA Translation: AAH83108.1
CCDSiCCDS40343.1
PIRiS26062
S54774
RefSeqiNP_032278.1, NM_008252.3
XP_006509587.1, XM_006509524.2
UniGeneiMm.276881
Mm.279998

Genome annotation databases

EnsembliENSMUST00000067925; ENSMUSP00000065940; ENSMUSG00000054717
GeneIDi97165
KEGGimmu:97165
UCSCiuc009lsx.2 mouse

Similar proteinsi

Entry informationi

Entry nameiHMGB2_MOUSE
AccessioniPrimary (citable) accession number: P30681
Secondary accession number(s): Q3UXT1, Q9EQD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again