UniProtKB - P30680 (SSR2_RAT)
Somatostatin receptor type 2
Sstr2
Functioni
Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization.
4 PublicationsGO - Molecular functioni
- G protein-coupled receptor activity Source: GO_Central
- neuropeptide binding Source: GO_Central
- PDZ domain binding Source: UniProtKB
- peptide binding Source: GO_Central
- somatostatin receptor activity Source: RGD
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: RGD
- cellular response to estradiol stimulus Source: RGD
- cellular response to glucocorticoid stimulus Source: RGD
- cerebellum development Source: RGD
- forebrain development Source: RGD
- G protein-coupled receptor signaling pathway Source: RGD
- neuropeptide signaling pathway Source: RGD
- peristalsis Source: RGD
- regulation of muscle contraction Source: RGD
- response to starvation Source: RGD
- spermatogenesis Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-375276, Peptide ligand-binding receptors R-RNO-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Somatostatin receptor type 2Short name: SS-2-R Short name: SS2-R Short name: SS2R Alternative name(s): SRIF-1 |
Gene namesi | Name:Sstr2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3763, Sstr2 |
Subcellular locationi
Plasma membrane
Cytoplasm and Cytosol
Note: Located mainly at the cell surface under basal conditions. Agonist stimulation results in internalization to the cytoplasm.
Cytosol
- cytosol Source: Ensembl
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: RGD
Other locations
- cytoplasm Source: RGD
- membrane Source: RGD
- neuron projection Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 43 | ExtracellularSequence analysisAdd BLAST | 43 | |
Transmembranei | 44 – 67 | Helical; Name=1Sequence analysisAdd BLAST | 24 | |
Topological domaini | 68 – 78 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 79 – 103 | Helical; Name=2Sequence analysisAdd BLAST | 25 | |
Topological domaini | 104 – 118 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 119 – 138 | Helical; Name=3Sequence analysisAdd BLAST | 20 | |
Topological domaini | 139 – 161 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 162 – 181 | Helical; Name=4Sequence analysisAdd BLAST | 20 | |
Topological domaini | 182 – 207 | ExtracellularSequence analysisAdd BLAST | 26 | |
Transmembranei | 208 – 229 | Helical; Name=5Sequence analysisAdd BLAST | 22 | |
Topological domaini | 230 – 253 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 254 – 278 | Helical; Name=6Sequence analysisAdd BLAST | 25 | |
Topological domaini | 279 – 288 | ExtracellularSequence analysis | 10 | |
Transmembranei | 289 – 303 | Helical; Name=7Sequence analysisAdd BLAST | 15 | |
Topological domaini | 304 – 369 | CytoplasmicSequence analysisAdd BLAST | 66 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL2978 |
DrugCentrali | P30680 |
GuidetoPHARMACOLOGYi | 356 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070123 | 1 – 369 | Somatostatin receptor type 2Add BLAST | 369 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 9 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 22 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 29 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 32 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 115 ↔ 193 | PROSITE-ProRule annotation | ||
Lipidationi | 328 | S-palmitoyl cysteineSequence analysis | 1 | |
Modified residuei | 341 | Phosphoserine1 Publication | 1 | |
Modified residuei | 343 | Phosphoserine1 Publication | 1 | |
Modified residuei | 348 | Phosphoserine1 Publication | 1 | |
Modified residuei | 353 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 354 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
PaxDbi | P30680 |
PTM databases
GlyGeni | P30680, 4 sites |
iPTMneti | P30680 |
PhosphoSitePlusi | P30680 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000002793, Expressed in brain and 13 other tissues |
Genevisiblei | P30680, RN |
Interactioni
Subunit structurei
Homodimer and heterodimer with SSTR3 and SSTR5. Heterodimerization with SSTR3 inactivates SSTR3 receptor function. Heterodimerization with SSTR5 is enhanced by agonist stimulation of SSTR2 and increases SSTR2 cell growth inhibition activity. Following agonist stimulation, homodimers dissociate into monomers which is required for receptor internalization.
Interacts with beta-arrestin; this interaction is necessary for receptor internalization and is destabilized by heterodimerization with SSTR5 which results in increased recycling of SSTR2 to the cell surface.
Interacts (via C-terminus) with SHANK1 (via PDZ domain).
2 PublicationsGO - Molecular functioni
- PDZ domain binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 248525, 6 interactors |
IntActi | P30680, 1 interactor |
MINTi | P30680 |
STRINGi | 10116.ENSRNOP00000003735 |
Chemistry databases
BindingDBi | P30680 |
Structurei
Secondary structure
3D structure databases
SMRi | P30680 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000156544 |
HOGENOMi | CLU_009579_8_1_1 |
InParanoidi | P30680 |
OMAi | KSRMIND |
OrthoDBi | 1011272at2759 |
PhylomeDBi | P30680 |
TreeFami | TF315737 |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR000586, Somatstn_rcpt IPR002074, Somatstn_rcpt_2 |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00246, SOMATOSTATNR PR00588, SOMATOSTTN2R |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELTSEQFNG SQVWIPSPFD LNGSLGPSNG SNQTEPYYDM TSNAVLTFIY
60 70 80 90 100
FVVCVVGLCG NTLVIYVILR YAKMKTITNI YILNLAIADE LFMLGLPFLA
110 120 130 140 150
MQVALVHWPF GKAICRVVMT VDGINQFTSI FCLTVMSIDR YLAVVHPIKS
160 170 180 190 200
AKWRRPRTAK MINVAVWGVS LLVILPIMIY AGLRSNQWGR SSCTINWPGE
210 220 230 240 250
SGAWYTGFII YAFILGFLVP LTIICLCYLF IIIKVKSSGI RVGSSKRKKS
260 270 280 290 300
EKKVTRMVSI VVAVFIFCWL PFYIFNVSSV SVAISPTPAL KGMFDFVVIL
310 320 330 340 350
TYANSCANPI LYAFLSDNFK KSFQNVLCLV KVSGAEDGER SDSKQDKSRL
360
NETTETQRTL LNGDLQTSI
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001924 | 332 – 369 | VSGAE…LQTSI → ADNSKTGEEDTMAWV in isoform B. CuratedAdd BLAST | 38 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96817 mRNA Translation: AAA42166.1 M93273 mRNA Translation: AAA42165.1 |
PIRi | A45291 |
RefSeqi | NP_062221.1, NM_019348.1 [P30680-1] |
Genome annotation databases
Ensembli | ENSRNOT00000003735; ENSRNOP00000003735; ENSRNOG00000002793 [P30680-1] |
GeneIDi | 54305 |
KEGGi | rno:54305 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96817 mRNA Translation: AAA42166.1 M93273 mRNA Translation: AAA42165.1 |
PIRi | A45291 |
RefSeqi | NP_062221.1, NM_019348.1 [P30680-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6EXJ | X-ray | 1.80 | B/D | 364-369 | [»] | |
SMRi | P30680 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 248525, 6 interactors |
IntActi | P30680, 1 interactor |
MINTi | P30680 |
STRINGi | 10116.ENSRNOP00000003735 |
Chemistry databases
BindingDBi | P30680 |
ChEMBLi | CHEMBL2978 |
DrugCentrali | P30680 |
GuidetoPHARMACOLOGYi | 356 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P30680, 4 sites |
iPTMneti | P30680 |
PhosphoSitePlusi | P30680 |
Proteomic databases
PaxDbi | P30680 |
Genome annotation databases
Ensembli | ENSRNOT00000003735; ENSRNOP00000003735; ENSRNOG00000002793 [P30680-1] |
GeneIDi | 54305 |
KEGGi | rno:54305 |
Organism-specific databases
CTDi | 6752 |
RGDi | 3763, Sstr2 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000156544 |
HOGENOMi | CLU_009579_8_1_1 |
InParanoidi | P30680 |
OMAi | KSRMIND |
OrthoDBi | 1011272at2759 |
PhylomeDBi | P30680 |
TreeFami | TF315737 |
Enzyme and pathway databases
Reactomei | R-RNO-375276, Peptide ligand-binding receptors R-RNO-418594, G alpha (i) signalling events |
Miscellaneous databases
PROi | PR:P30680 |
Gene expression databases
Bgeei | ENSRNOG00000002793, Expressed in brain and 13 other tissues |
Genevisiblei | P30680, RN |
Family and domain databases
InterProi | View protein in InterPro IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR000586, Somatstn_rcpt IPR002074, Somatstn_rcpt_2 |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00237, GPCRRHODOPSN PR00246, SOMATOSTATNR PR00588, SOMATOSTTN2R |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SSR2_RAT | |
Accessioni | P30680Primary (citable) accession number: P30680 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | September 29, 2021 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families