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Protein

Somatostatin receptor type 2

Gene

Sstr2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for somatostatin-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. Inhibits calcium entry by suppressing voltage-dependent calcium channels. Acts as the functionally dominant somatostatin receptor in pancreatic alpha- and beta-cells where it mediates the inhibitory effect of somatostatin-14 on hormone secretion. Inhibits cell growth through enhancement of MAPK1 and MAPK2 phosphorylation and subsequent up-regulation of CDKN1B. Stimulates neuronal migration and axon outgrowth and may participate in neuron development and maturation during brain development. Mediates negative regulation of insulin receptor signaling through PTPN6. Inactivates SSTR3 receptor function following heterodimerization.4 Publications

GO - Molecular functioni

  • G protein-coupled receptor activity Source: GO_Central
  • neuropeptide binding Source: GO_Central
  • PDZ domain binding Source: UniProtKB
  • somatostatin receptor activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-375276 Peptide ligand-binding receptors
R-RNO-418594 G alpha (i) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Somatostatin receptor type 2
Short name:
SS-2-R
Short name:
SS2-R
Short name:
SS2R
Alternative name(s):
SRIF-1
Gene namesi
Name:Sstr2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3763 Sstr2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43ExtracellularSequence analysisAdd BLAST43
Transmembranei44 – 67Helical; Name=1Sequence analysisAdd BLAST24
Topological domaini68 – 78CytoplasmicSequence analysisAdd BLAST11
Transmembranei79 – 103Helical; Name=2Sequence analysisAdd BLAST25
Topological domaini104 – 118ExtracellularSequence analysisAdd BLAST15
Transmembranei119 – 138Helical; Name=3Sequence analysisAdd BLAST20
Topological domaini139 – 161CytoplasmicSequence analysisAdd BLAST23
Transmembranei162 – 181Helical; Name=4Sequence analysisAdd BLAST20
Topological domaini182 – 207ExtracellularSequence analysisAdd BLAST26
Transmembranei208 – 229Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini230 – 253CytoplasmicSequence analysisAdd BLAST24
Transmembranei254 – 278Helical; Name=6Sequence analysisAdd BLAST25
Topological domaini279 – 288ExtracellularSequence analysis10
Transmembranei289 – 303Helical; Name=7Sequence analysisAdd BLAST15
Topological domaini304 – 369CytoplasmicSequence analysisAdd BLAST66

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2978
GuidetoPHARMACOLOGYi356

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000701231 – 369Somatostatin receptor type 2Add BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi9N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi22N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi29N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi32N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi115 ↔ 193PROSITE-ProRule annotation
Lipidationi328S-palmitoyl cysteineSequence analysis1
Modified residuei341Phosphoserine1 Publication1
Modified residuei343Phosphoserine1 Publication1
Modified residuei348Phosphoserine1 Publication1
Modified residuei353Phosphothreonine1 Publication1
Modified residuei354Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated on serine and threonine residues in response to agonist stimulation, leading to receptor desensitization and rapid internalization. Phosphorylated to a greater extent on serine than threonine residues. Threonine phosphorylation is required for arrestin binding and receptor endocytosis but is not necessary for desensitization.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP30680
PRIDEiP30680

PTM databases

iPTMnetiP30680
PhosphoSitePlusiP30680

Expressioni

Tissue specificityi

Cortex, hippocampus, pituitary gland, colon, kidney, and adrenal gland. In the developing nervous system, expressed from E12 when it is restricted to postmitotic neuronal populations leaving the ventricular zone. From E12 on, expressed in migrating neuronal populations in numerous developing regions including the cerebral cortex, hippocampus and ganglionic eminences. Also detected in the deep part of the external granular layer of the cerebellum, the rostral migratory stream and a subset of axons and neurons. Expressed in the medial forebrain bundle, rostral migratory stream and cerebellum during development but not in adulthood.3 Publications

Gene expression databases

BgeeiENSRNOG00000002793 Expressed in 3 organ(s), highest expression level in Ammon's horn
GenevisibleiP30680 RN

Interactioni

Subunit structurei

Homodimer and heterodimer with SSTR3 and SSTR5. Heterodimerization with SSTR3 inactivates SSTR3 receptor function. Heterodimerization with SSTR5 is enhanced by agonist stimulation of SSTR2 and increases SSTR2 cell growth inhibition activity. Following agonist stimulation, homodimers dissociate into monomers which is required for receptor internalization. Interacts with beta-arrestin; this interaction is necessary for receptor internalization and is destabilized by heterodimerization with SSTR5 which results in increased recycling of SSTR2 to the cell surface. Interacts (via C-terminus) with SHANK1 (via PDZ domain).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248525, 4 interactors
IntActiP30680, 1 interactor
MINTiP30680
STRINGi10116.ENSRNOP00000003735

Chemistry databases

BindingDBiP30680

Structurei

Secondary structure

1369
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP30680
SMRiP30680
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118797
HOGENOMiHOG000230485
HOVERGENiHBG106919
InParanoidiP30680
KOiK04218
OMAiTIVWPEP
OrthoDBiEOG091G0HEN
PhylomeDBiP30680
TreeFamiTF315737

Family and domain databases

CDDicd15971 7tmA_SSTR2, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR000586 Somatstn_rcpt
IPR002074 Somatstn_rcpt_2
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00246 SOMATOSTATNR
PR00588 SOMATOSTTN2R
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform A (identifier: P30680-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELTSEQFNG SQVWIPSPFD LNGSLGPSNG SNQTEPYYDM TSNAVLTFIY
60 70 80 90 100
FVVCVVGLCG NTLVIYVILR YAKMKTITNI YILNLAIADE LFMLGLPFLA
110 120 130 140 150
MQVALVHWPF GKAICRVVMT VDGINQFTSI FCLTVMSIDR YLAVVHPIKS
160 170 180 190 200
AKWRRPRTAK MINVAVWGVS LLVILPIMIY AGLRSNQWGR SSCTINWPGE
210 220 230 240 250
SGAWYTGFII YAFILGFLVP LTIICLCYLF IIIKVKSSGI RVGSSKRKKS
260 270 280 290 300
EKKVTRMVSI VVAVFIFCWL PFYIFNVSSV SVAISPTPAL KGMFDFVVIL
310 320 330 340 350
TYANSCANPI LYAFLSDNFK KSFQNVLCLV KVSGAEDGER SDSKQDKSRL
360
NETTETQRTL LNGDLQTSI
Length:369
Mass (Da):41,200
Last modified:April 1, 1993 - v1
Checksum:i4990E489E88D7D19
GO
Isoform B (identifier: P30680-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     332-369: VSGAEDGERSDSKQDKSRLNETTETQRTLLNGDLQTSI → ADNSKTGEEDTMAWV

Show »
Length:346
Mass (Da):38,673
Checksum:i2D75370906A660E3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001924332 – 369VSGAE…LQTSI → ADNSKTGEEDTMAWV in isoform B. CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96817 mRNA Translation: AAA42166.1
M93273 mRNA Translation: AAA42165.1
PIRiA45291
RefSeqiNP_062221.1, NM_019348.1 [P30680-1]
UniGeneiRn.202974
Rn.9929

Genome annotation databases

EnsembliENSRNOT00000003735; ENSRNOP00000003735; ENSRNOG00000002793 [P30680-1]
GeneIDi54305
KEGGirno:54305

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96817 mRNA Translation: AAA42166.1
M93273 mRNA Translation: AAA42165.1
PIRiA45291
RefSeqiNP_062221.1, NM_019348.1 [P30680-1]
UniGeneiRn.202974
Rn.9929

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6EXJX-ray1.80B/D364-369[»]
ProteinModelPortaliP30680
SMRiP30680
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248525, 4 interactors
IntActiP30680, 1 interactor
MINTiP30680
STRINGi10116.ENSRNOP00000003735

Chemistry databases

BindingDBiP30680
ChEMBLiCHEMBL2978
GuidetoPHARMACOLOGYi356

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP30680
PhosphoSitePlusiP30680

Proteomic databases

PaxDbiP30680
PRIDEiP30680

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003735; ENSRNOP00000003735; ENSRNOG00000002793 [P30680-1]
GeneIDi54305
KEGGirno:54305

Organism-specific databases

CTDi6752
RGDi3763 Sstr2

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118797
HOGENOMiHOG000230485
HOVERGENiHBG106919
InParanoidiP30680
KOiK04218
OMAiTIVWPEP
OrthoDBiEOG091G0HEN
PhylomeDBiP30680
TreeFamiTF315737

Enzyme and pathway databases

ReactomeiR-RNO-375276 Peptide ligand-binding receptors
R-RNO-418594 G alpha (i) signalling events

Miscellaneous databases

PROiPR:P30680

Gene expression databases

BgeeiENSRNOG00000002793 Expressed in 3 organ(s), highest expression level in Ammon's horn
GenevisibleiP30680 RN

Family and domain databases

CDDicd15971 7tmA_SSTR2, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR000586 Somatstn_rcpt
IPR002074 Somatstn_rcpt_2
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00246 SOMATOSTATNR
PR00588 SOMATOSTTN2R
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSSR2_RAT
AccessioniPrimary (citable) accession number: P30680
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 7, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
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Main funding by: National Institutes of Health

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