UniProtKB - P30665 (MCM4_YEAST)
DNA replication licensing factor MCM4
MCM4
Functioni
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Required for S phase execution.
2 PublicationsMiscellaneous
Catalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 568 – 575 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- DNA helicase activity Source: UniProtKB-EC
- DNA replication origin binding Source: SGD
- helicase activity Source: Reactome
- single-stranded DNA binding Source: SGD
GO - Biological processi
- DNA replication initiation Source: SGD
- DNA strand elongation involved in DNA replication Source: SGD
- DNA unwinding involved in DNA replication Source: SGD
- double-strand break repair via break-induced replication Source: SGD
- mitotic DNA replication initiation Source: GO_Central
- nuclear DNA replication Source: SGD
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
- protein hexamerization Source: SGD
- regulation of DNA-dependent DNA replication initiation Source: ComplexPortal
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-68949, Orc1 removal from chromatin R-SCE-68962, Activation of the pre-replicative complex R-SCE-69052, Switching of origins to a post-replicative state |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM4 (EC:3.6.4.12)Alternative name(s): Cell division control protein 54 |
Gene namesi | Name:MCM4 Synonyms:CDC54, HCD21 Ordered Locus Names:YPR019W ORF Names:YP9531.13 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006223, MCM4 |
VEuPathDBi | FungiDB:YPR019W |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- CMG complex Source: SGD
- DNA replication preinitiation complex Source: SGD
- nuclear pre-replicative complex Source: SGD
- nuclear replication fork Source: SGD
- nucleoplasm Source: Reactome
- nucleus Source: SGD
- replication fork protection complex Source: SGD
Other locations
- cytoplasm Source: SGD
- MCM complex Source: SGD
- MCM core complex Source: SGD
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 574 | K → A: Loss of MCM2-7 complex helicase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194105 | 1 – 933 | DNA replication licensing factor MCM4Add BLAST | 933 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 52 | PhosphoserineCombined sources | 1 | |
Modified residuei | 56 | PhosphoserineCombined sources | 1 | |
Modified residuei | 69 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P30665 |
PaxDbi | P30665 |
PRIDEi | P30665 |
PTM databases
iPTMneti | P30665 |
Interactioni
Subunit structurei
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5; loaded onto DNA, forms a head-head double hexamer.
2 PublicationsBinary interactionsi
P30665
Protein-protein interaction databases
BioGRIDi | 36196, 162 interactors |
ComplexPortali | CPX-2944, MCM complex |
DIPi | DIP-2409N |
IntActi | P30665, 35 interactors |
MINTi | P30665 |
STRINGi | 4932.YPR019W |
Miscellaneous databases
RNActi | P30665, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P30665 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 518 – 725 | MCMAdd BLAST | 208 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 149 | DisorderedSequence analysisAdd BLAST | 149 | |
Regioni | 154 – 173 | DisorderedSequence analysisAdd BLAST | 20 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 700 – 703 | Arginine finger | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 20 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 29 – 105 | Polar residuesSequence analysisAdd BLAST | 77 | |
Compositional biasi | 113 – 129 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 130 – 149 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 156 – 172 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0478, Eukaryota |
GeneTreei | ENSGT01040000240446 |
HOGENOMi | CLU_000995_7_1_1 |
InParanoidi | P30665 |
OMAi | KNTIRIC |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR031327, MCM IPR008047, MCM_4 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR11630, PTHR11630, 1 hit PTHR11630:SF66, PTHR11630:SF66, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01660, MCMPROTEIN4 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSQQSSSPTK EDNNSSSPVV PNPDSVPPQL SSPALFYSSS SSQGDIYGRN
60 70 80 90 100
NSQNLSQGEG NIRAAIGSSP LNFPSSSQRQ NSDVFQSQGR QGRIRSSASA
110 120 130 140 150
SGRSRYHSDL RSDRALPTSS SSLGRNGQNR VHMRRNDIHT SDLSSPRRIV
160 170 180 190 200
DFDTRSGVNT LDTSSSSAPP SEASEPLRII WGTNVSIQEC TTNFRNFLMS
210 220 230 240 250
FKYKFRKILD EREEFINNTT DEELYYIKQL NEMRELGTSN LNLDARNLLA
260 270 280 290 300
YKQTEDLYHQ LLNYPQEVIS IMDQTIKDCM VSLIVDNNLD YDLDEIETKF
310 320 330 340 350
YKVRPYNVGS CKGMRELNPN DIDKLINLKG LVLRSTPVIP DMKVAFFKCN
360 370 380 390 400
VCDHTMAVEI DRGVIQEPAR CERIDCNEPN SMSLIHNRCS FADKQVIKLQ
410 420 430 440 450
ETPDFVPDGQ TPHSISLCVY DELVDSCRAG DRIEVTGTFR SIPIRANSRQ
460 470 480 490 500
RVLKSLYKTY VDVVHVKKVS DKRLDVDTST IEQELMQNKV DHNEVEEVRQ
510 520 530 540 550
ITDQDLAKIR EVAAREDLYS LLARSIAPSI YELEDVKKGI LLQLFGGTNK
560 570 580 590 600
TFTKGGRYRG DINILLCGDP STSKSQILQY VHKITPRGVY TSGKGSSAVG
610 620 630 640 650
LTAYITRDVD TKQLVLESGA LVLSDGGVCC IDEFDKMSDS TRSVLHEVME
660 670 680 690 700
QQTISIAKAG IITTLNARSS ILASANPIGS RYNPNLPVTE NIDLPPPLLS
710 720 730 740 750
RFDLVYLVLD KVDEKNDREL AKHLTNLYLE DKPEHISQDD VLPVEFLTMY
760 770 780 790 800
ISYAKEHIHP IITEAAKTEL VRAYVGMRKM GDDSRSDEKR ITATTRQLES
810 820 830 840 850
MIRLAEAHAK MKLKNVVELE DVQEAVRLIR SAIKDYATDP KTGKIDMNLV
860 870 880 890 900
QTGKSVIQRK LQEDLSREIM NVLKDQASDS MSFNELIKQI NEHSQDRVES
910 920 930
SDIQEALSRL QQEDKVIVLG EGVRRSVRLN NRV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14731 Genomic DNA Translation: AAA86310.1 Z49919 Genomic DNA Translation: CAA90164.1 Z71255 Genomic DNA Translation: CAA95015.1 Z15032 Genomic DNA Translation: CAA78750.1 BK006949 Genomic DNA Translation: DAA11445.1 |
PIRi | S56050 |
RefSeqi | NP_015344.1, NM_001184116.1 |
Genome annotation databases
EnsemblFungii | YPR019W_mRNA; YPR019W; YPR019W |
GeneIDi | 856130 |
KEGGi | sce:YPR019W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14731 Genomic DNA Translation: AAA86310.1 Z49919 Genomic DNA Translation: CAA90164.1 Z71255 Genomic DNA Translation: CAA95015.1 Z15032 Genomic DNA Translation: CAA78750.1 BK006949 Genomic DNA Translation: DAA11445.1 |
PIRi | S56050 |
RefSeqi | NP_015344.1, NM_001184116.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JA8 | electron microscopy | 3.80 | 4 | 1-933 | [»] | |
3JC5 | electron microscopy | 4.70 | 4 | 1-933 | [»] | |
3JC6 | electron microscopy | 3.70 | 4 | 1-933 | [»] | |
3JC7 | electron microscopy | 4.80 | 4 | 1-933 | [»] | |
5BK4 | electron microscopy | 3.90 | 4/C | 1-933 | [»] | |
5U8S | electron microscopy | 6.10 | 4 | 1-933 | [»] | |
5U8T | electron microscopy | 4.90 | 4 | 1-933 | [»] | |
5V8F | electron microscopy | 3.90 | 4 | 1-933 | [»] | |
5XF8 | electron microscopy | 7.10 | 4 | 1-933 | [»] | |
6EYC | electron microscopy | 3.80 | 4 | 1-933 | [»] | |
6F0L | electron microscopy | 4.77 | 4/C | 1-933 | [»] | |
6HV9 | electron microscopy | 4.98 | 4 | 1-933 | [»] | |
6PTJ | electron microscopy | 3.80 | 4 | 1-933 | [»] | |
6PTN | electron microscopy | 5.80 | 4/k | 1-933 | [»] | |
6PTO | electron microscopy | 7.00 | 4/H/j | 1-933 | [»] | |
6RQC | electron microscopy | 4.40 | 4 | 1-933 | [»] | |
6SKL | electron microscopy | 3.70 | 4 | 1-933 | [»] | |
6SKO | electron microscopy | 3.40 | 4 | 1-933 | [»] | |
6U0M | electron microscopy | 3.90 | 4 | 177-929 | [»] | |
6WGF | electron microscopy | 7.70 | 4 | 1-933 | [»] | |
6WGG | electron microscopy | 8.10 | 4 | 1-933 | [»] | |
6WGI | electron microscopy | 10.00 | 4 | 1-933 | [»] | |
SMRi | P30665 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36196, 162 interactors |
ComplexPortali | CPX-2944, MCM complex |
DIPi | DIP-2409N |
IntActi | P30665, 35 interactors |
MINTi | P30665 |
STRINGi | 4932.YPR019W |
PTM databases
iPTMneti | P30665 |
Proteomic databases
MaxQBi | P30665 |
PaxDbi | P30665 |
PRIDEi | P30665 |
Genome annotation databases
EnsemblFungii | YPR019W_mRNA; YPR019W; YPR019W |
GeneIDi | 856130 |
KEGGi | sce:YPR019W |
Organism-specific databases
SGDi | S000006223, MCM4 |
VEuPathDBi | FungiDB:YPR019W |
Phylogenomic databases
eggNOGi | KOG0478, Eukaryota |
GeneTreei | ENSGT01040000240446 |
HOGENOMi | CLU_000995_7_1_1 |
InParanoidi | P30665 |
OMAi | KNTIRIC |
Enzyme and pathway databases
Reactomei | R-SCE-68949, Orc1 removal from chromatin R-SCE-68962, Activation of the pre-replicative complex R-SCE-69052, Switching of origins to a post-replicative state |
Miscellaneous databases
PROi | PR:P30665 |
RNActi | P30665, protein |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR031327, MCM IPR008047, MCM_4 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR11630, PTHR11630, 1 hit PTHR11630:SF66, PTHR11630:SF66, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01660, MCMPROTEIN4 |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM4_YEAST | |
Accessioni | P30665Primary (citable) accession number: P30665 Secondary accession number(s): D6W429 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 201 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families