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Protein

Chromobox protein homolog 2

Gene

Cbx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (By similarity). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3) (PubMed:16537902). Plays a role in the lineage differentiation of the germ layers in embryonic development (PubMed:22226355). Involved in sexual development, acting as activator of NR5A1 expression (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi75 – 87A.T hookAdd BLAST13

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: UniProtKB-KW
  • methylated histone binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2559580 Oxidative Stress Induced Senescence
R-MMU-3108214 SUMOylation of DNA damage response and repair proteins
R-MMU-3899300 SUMOylation of transcription cofactors
R-MMU-4551638 SUMOylation of chromatin organization proteins
R-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known
R-MMU-8943724 Regulation of PTEN gene transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 2
Alternative name(s):
M33
Modifier 3 protein
Gene namesi
Name:Cbx2
Synonyms:M33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:88289 Cbx2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802021 – 519Chromobox protein homolog 2Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki147Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei248Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei248Omega-N-methylarginine; alternateCombined sources1
Modified residuei303PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP30658
MaxQBiP30658
PaxDbiP30658
PeptideAtlasiP30658
PRIDEiP30658

PTM databases

iPTMnetiP30658
PhosphoSitePlusiP30658

Expressioni

Tissue specificityi

Expressed in embryoid bodies.1 Publication

Developmental stagei

Expressed in most embryonic tissues except the heart from 8.5 to 11.5 dpc. Expressed in central nervous system (CNS, ventricular zone and spinal cord), peripheral nervous system (PNS, sensory cranial and spinal ganglia), olfactory and tongue epithelia, lung, gastrointestinal duct and urogenital system at 13.5 dpc. Expressed in CNS, thymus, various epithelial cell types including the olfactory, tooth and tongue epithelia at 15.5 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000025577 Expressed in 214 organ(s), highest expression level in primary oocyte
CleanExiMM_CBX2
GenevisibleiP30658 MM

Interactioni

Subunit structurei

Component of a PRC1-like complex (By similarity). The composition of the PRC1 complex may differ between the PRC1 complex in pluripotent embryonic stem cells containing RNF2, CBX7 and PCGF2, and the PRC1 complex in differentiating cells containing RNF2, CBX2, CBX4 and BMI1 (PubMed:22226355). Interacts with RING1/RNF2 (PubMed:9312051, PubMed:22226355). Interacts (via chromodomain) with histone H3K9Me3 and H3K27me3 (PubMed:16537902). May interact with HIST2H3A and HIST1H3A (By similarity).By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198535, 19 interactors
CORUMiP30658
DIPiDIP-690N
IntActiP30658, 17 interactors
MINTiP30658
STRINGi10090.ENSMUSP00000026662

Structurei

3D structure databases

ProteinModelPortaliP30658
SMRiP30658
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 70ChromoPROSITE-ProRule annotationAdd BLAST59

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi164 – 169Nuclear localization signalSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi102 – 120Ser-richAdd BLAST19
Compositional biasi121 – 130Asp/Glu-rich (acidic)10

Phylogenomic databases

eggNOGiENOG410IPQ5 Eukaryota
ENOG4111HE0 LUCA
GeneTreeiENSGT00530000063056
HOGENOMiHOG000231217
HOVERGENiHBG005256
InParanoidiP30658
KOiK11451
OMAiKCGLGLD
OrthoDBiEOG091G08J1
TreeFamiTF106456

Family and domain databases

CDDicd00024 CHROMO, 1 hit
InterProiView protein in InterPro
IPR033773 CBX7_C
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR023780 Chromo_domain
IPR023779 Chromodomain_CS
PfamiView protein in Pfam
PF17218 CBX7_C, 1 hit
PF00385 Chromo, 1 hit
SMARTiView protein in SMART
SM00298 CHROMO, 1 hit
SUPFAMiSSF54160 SSF54160, 1 hit
PROSITEiView protein in PROSITE
PS00598 CHROMO_1, 1 hit
PS50013 CHROMO_2, 1 hit

Sequencei

Sequence statusi: Complete.

P30658-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEELSSVGEQ VFAAECILSK RLRKGKLEYL VKWRGWSSKH NSWEPEENIL
60 70 80 90 100
DPRLLLAFQK KEHEKEVQNR KRGKRPRGRP RKHTVTSSCS RRSKLKEPDA
110 120 130 140 150
PSKSKSSSSS SSSTSSSSSS DEEEDDSDLD SKRGPRGRET HPVPQKKAQI
160 170 180 190 200
LVAKPELKDP IRKKRGRKPL PPEQKAARRP VSLAKVLKTT RKDLGTSAAK
210 220 230 240 250
LPPPLSAPVA GLAALKAHTK EACGGPSTMA TPENLASLMK GMAGSPSRGG
260 270 280 290 300
IWQSSIVHYM NRMSQSQVQA ASRLALKAQA TNKCGLGLDL KVRTQKGGEL
310 320 330 340 350
GGSPAGGKVP KAPGGGAAEQ QRGNHSGSPG AQLAPTQELS LQVLDLQSVK
360 370 380 390 400
NGVPGVGLLA RHAPAKAIPA TNPATGKGPG SGPTGANMTN APTDNNKGEK
410 420 430 440 450
LTCKATALPA PSVKRDTVKS VAASGGQEGH TAPGEGRKPP ALSELSTGEE
460 470 480 490 500
NSSSDSDPDS TSLPSAAQNL SVAIQTSQDW KPTRSLIEHV FVTDVTANLI
510
TVTVKESPTS VGFFNLRHY
Length:519
Mass (Da):54,918
Last modified:July 27, 2011 - v2
Checksum:iF89EF3CEF14ACA72
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → EF in CAA73723 (PubMed:9312051).Curated1
Sequence conflicti85V → A in CAA44398 (PubMed:1352241).Curated1
Sequence conflicti107S → G in CAA73723 (PubMed:9312051).Curated1
Sequence conflicti133R → G in CAA73723 (PubMed:9312051).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62537 mRNA Translation: CAA44398.1
Y13274 mRNA Translation: CAA73723.1
AL662835 Genomic DNA Translation: CAM24931.1
CH466558 Genomic DNA Translation: EDL34680.1
BC035199 mRNA Translation: AAH35199.1
CCDSiCCDS25708.1
PIRiS23796
RefSeqiNP_031649.2, NM_007623.3
UniGeneiMm.14547

Genome annotation databases

EnsembliENSMUST00000026662; ENSMUSP00000026662; ENSMUSG00000025577
GeneIDi12416
KEGGimmu:12416
UCSCiuc007mpt.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62537 mRNA Translation: CAA44398.1
Y13274 mRNA Translation: CAA73723.1
AL662835 Genomic DNA Translation: CAM24931.1
CH466558 Genomic DNA Translation: EDL34680.1
BC035199 mRNA Translation: AAH35199.1
CCDSiCCDS25708.1
PIRiS23796
RefSeqiNP_031649.2, NM_007623.3
UniGeneiMm.14547

3D structure databases

ProteinModelPortaliP30658
SMRiP30658
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198535, 19 interactors
CORUMiP30658
DIPiDIP-690N
IntActiP30658, 17 interactors
MINTiP30658
STRINGi10090.ENSMUSP00000026662

PTM databases

iPTMnetiP30658
PhosphoSitePlusiP30658

Proteomic databases

EPDiP30658
MaxQBiP30658
PaxDbiP30658
PeptideAtlasiP30658
PRIDEiP30658

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026662; ENSMUSP00000026662; ENSMUSG00000025577
GeneIDi12416
KEGGimmu:12416
UCSCiuc007mpt.1 mouse

Organism-specific databases

CTDi84733
MGIiMGI:88289 Cbx2

Phylogenomic databases

eggNOGiENOG410IPQ5 Eukaryota
ENOG4111HE0 LUCA
GeneTreeiENSGT00530000063056
HOGENOMiHOG000231217
HOVERGENiHBG005256
InParanoidiP30658
KOiK11451
OMAiKCGLGLD
OrthoDBiEOG091G08J1
TreeFamiTF106456

Enzyme and pathway databases

ReactomeiR-MMU-2559580 Oxidative Stress Induced Senescence
R-MMU-3108214 SUMOylation of DNA damage response and repair proteins
R-MMU-3899300 SUMOylation of transcription cofactors
R-MMU-4551638 SUMOylation of chromatin organization proteins
R-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known
R-MMU-8943724 Regulation of PTEN gene transcription

Miscellaneous databases

ChiTaRSiCbx2 mouse
PROiPR:P30658
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025577 Expressed in 214 organ(s), highest expression level in primary oocyte
CleanExiMM_CBX2
GenevisibleiP30658 MM

Family and domain databases

CDDicd00024 CHROMO, 1 hit
InterProiView protein in InterPro
IPR033773 CBX7_C
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR023780 Chromo_domain
IPR023779 Chromodomain_CS
PfamiView protein in Pfam
PF17218 CBX7_C, 1 hit
PF00385 Chromo, 1 hit
SMARTiView protein in SMART
SM00298 CHROMO, 1 hit
SUPFAMiSSF54160 SSF54160, 1 hit
PROSITEiView protein in PROSITE
PS00598 CHROMO_1, 1 hit
PS50013 CHROMO_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCBX2_MOUSE
AccessioniPrimary (citable) accession number: P30658
Secondary accession number(s): A2ABG2, O35731, Q8CIA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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