UniProtKB - P30655 (PSB5_SCHPO)
Probable proteasome subunit beta type-5
pts1
Functioni
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activityi
- Cleavage of peptide bonds with very broad specificity. EC:3.4.25.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 62 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- endopeptidase activity Source: GO_Central
- threonine-type endopeptidase activity Source: UniProtKB-KW
GO - Biological processi
- mitotic sister chromatid separation Source: PomBase
- proteasomal protein catabolic process Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: PomBase
Keywordsi
Molecular function | Hydrolase, Protease, Threonine protease |
Enzyme and pathway databases
Reactomei | R-SPO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SPO-174113, SCF-beta-TrCP mediated degradation of Emi1 R-SPO-350562, Regulation of ornithine decarboxylase (ODC) R-SPO-382556, ABC-family proteins mediated transport R-SPO-5668541, TNFR2 non-canonical NF-kB pathway R-SPO-5689603, UCH proteinases R-SPO-5689880, Ub-specific processing proteases R-SPO-68949, Orc1 removal from chromatin R-SPO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-SPO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-SPO-75815, Ubiquitin-dependent degradation of Cyclin D R-SPO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-SPO-8948751, Regulation of PTEN stability and activity R-SPO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Protein family/group databases
MEROPSi | T01.012 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable proteasome subunit beta type-5 (EC:3.4.25.1)Alternative name(s): Macropain subunit pts1 Multicatalytic endopeptidase complex subunit pts1 Proteasome component pts1 |
Gene namesi | Name:pts1 ORF Names:SPAC4A8.13c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC4A8.13c, pts1 |
VEuPathDBi | FungiDB:SPAC4A8.13c |
Subcellular locationi
Cytosol
- cytosol Source: PomBase
Nucleus
- nuclear envelope Source: PomBase
- nucleus Source: PomBase
Other locations
- proteasome core complex Source: GO_Central
- proteasome core complex, beta-subunit complex Source: PomBase
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000026609 | 1 – 61 | Removed in mature formBy similarityAdd BLAST | 61 | |
ChainiPRO_0000026610 | 62 – 272 | Probable proteasome subunit beta type-5Add BLAST | 211 |
Keywords - PTMi
ZymogenProteomic databases
MaxQBi | P30655 |
PaxDbi | P30655 |
PTM databases
iPTMneti | P30655 |
Interactioni
Subunit structurei
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 280037, 5 interactors |
STRINGi | 4896.SPAC4A8.13c.1 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0175, Eukaryota |
HOGENOMi | CLU_035750_7_1_1 |
InParanoidi | P30655 |
OMAi | NLGMAMQ |
PhylomeDBi | P30655 |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR000243, Pept_T1A_subB IPR035705, Proteasome_beta8 IPR016050, Proteasome_bsu_CS IPR001353, Proteasome_sua/b IPR023333, Proteasome_suB-type |
PANTHERi | PTHR11599:SF53, PTHR11599:SF53, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit |
PRINTSi | PR00141, PROTEASOME |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00854, PROTEASOME_BETA_1, 1 hit PS51476, PROTEASOME_BETA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MNSIVSKYTQ STNNDDPKKI IEEEGFTNRF DVVPVPQSSL YLRNLTDETK
60 70 80 90 100
NKHCLIKMNH GTTTLAFRYQ HGIVVCVDSR ASAGPLIASQ TVKKVIEINP
110 120 130 140 150
YLLGTLAGGA ADCQFWETVL GMECRLHQLR NKELISVSAA SKILSNITYS
160 170 180 190 200
YKGYGLSMGT MLAGTGKGGT ALYYIDSDGT RLKGDLFSVG SGSTFAYGVL
210 220 230 240 250
DSGYRWDLSK QEALYLAQRS IVAATHRDAY SGGSVNLYHI DENGWVFHGN
260 270
FDVDSLIWEA KDNENSFAHI PR
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13094 Genomic DNA Translation: BAA02403.1 CU329670 Genomic DNA Translation: CAB11483.1 X83866 Genomic DNA Translation: CAA58746.1 Different initiation. |
PIRi | JS0753 T38783 |
RefSeqi | NP_593825.1, NM_001019254.2 |
Genome annotation databases
EnsemblFungii | SPAC4A8.13c.1; SPAC4A8.13c.1:pep; SPAC4A8.13c |
GeneIDi | 2543623 |
KEGGi | spo:SPAC4A8.13c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13094 Genomic DNA Translation: BAA02403.1 CU329670 Genomic DNA Translation: CAB11483.1 X83866 Genomic DNA Translation: CAA58746.1 Different initiation. |
PIRi | JS0753 T38783 |
RefSeqi | NP_593825.1, NM_001019254.2 |
3D structure databases
AlphaFoldDBi | P30655 |
SMRi | P30655 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 280037, 5 interactors |
STRINGi | 4896.SPAC4A8.13c.1 |
Protein family/group databases
MEROPSi | T01.012 |
PTM databases
iPTMneti | P30655 |
Proteomic databases
MaxQBi | P30655 |
PaxDbi | P30655 |
Genome annotation databases
EnsemblFungii | SPAC4A8.13c.1; SPAC4A8.13c.1:pep; SPAC4A8.13c |
GeneIDi | 2543623 |
KEGGi | spo:SPAC4A8.13c |
Organism-specific databases
PomBasei | SPAC4A8.13c, pts1 |
VEuPathDBi | FungiDB:SPAC4A8.13c |
Phylogenomic databases
eggNOGi | KOG0175, Eukaryota |
HOGENOMi | CLU_035750_7_1_1 |
InParanoidi | P30655 |
OMAi | NLGMAMQ |
PhylomeDBi | P30655 |
Enzyme and pathway databases
Reactomei | R-SPO-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SPO-174113, SCF-beta-TrCP mediated degradation of Emi1 R-SPO-350562, Regulation of ornithine decarboxylase (ODC) R-SPO-382556, ABC-family proteins mediated transport R-SPO-5668541, TNFR2 non-canonical NF-kB pathway R-SPO-5689603, UCH proteinases R-SPO-5689880, Ub-specific processing proteases R-SPO-68949, Orc1 removal from chromatin R-SPO-69017, CDK-mediated phosphorylation and removal of Cdc6 R-SPO-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-SPO-75815, Ubiquitin-dependent degradation of Cyclin D R-SPO-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-SPO-8948751, Regulation of PTEN stability and activity R-SPO-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:P30655 |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR000243, Pept_T1A_subB IPR035705, Proteasome_beta8 IPR016050, Proteasome_bsu_CS IPR001353, Proteasome_sua/b IPR023333, Proteasome_suB-type |
PANTHERi | PTHR11599:SF53, PTHR11599:SF53, 1 hit |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit |
PRINTSi | PR00141, PROTEASOME |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00854, PROTEASOME_BETA_1, 1 hit PS51476, PROTEASOME_BETA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PSB5_SCHPO | |
Accessioni | P30655Primary (citable) accession number: P30655 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | July 15, 1998 | |
Last modified: | May 25, 2022 | |
This is version 171 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families