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Protein

Thiamin pyrophosphokinase 1

Gene

tpk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Functions cell non-autonomously.1 Publication

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamin pyrophosphokinase 1 (tpk-1)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.6.2 1045
ReactomeiR-CEL-196819 Vitamin B1 (thiamin) metabolism
UniPathwayiUPA00060; UER00597

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamin pyrophosphokinase 1 (EC:2.7.6.2)
Alternative name(s):
Thiamine pyrophosphokinase 1
Short name:
TPK1
Gene namesi
Name:tpk-1
ORF Names:ZK637.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiZK637.9a ; CE37946 ; WBGene00014027 ; tpk-1
ZK637.9b ; CE37947 ; WBGene00014027 ; tpk-1

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67C → Y in qm162; maternal-effect mutation. Partial loss-of-function. Slowdown and deregulation of physiological rhythms and lengthening of lifespan. No effect on oxygen consumption rate. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726461 – 243Thiamin pyrophosphokinase 1Add BLAST243

Proteomic databases

EPDiP30636
PaxDbiP30636
PeptideAtlasiP30636

Expressioni

Gene expression databases

BgeeiWBGene00014027

Interactioni

Protein-protein interaction databases

STRINGi6239.ZK637.9b

Structurei

3D structure databases

ProteinModelPortaliP30636
SMRiP30636
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiKOG3153 Eukaryota
COG1564 LUCA
GeneTreeiENSGT00390000016016
HOGENOMiHOG000018120
InParanoidiP30636
KOiK00949
OMAiTGNLKYC
OrthoDBiEOG091G0FS8
PhylomeDBiP30636

Family and domain databases

CDDicd07995 TPK, 1 hit
Gene3Di3.40.50.10240, 1 hit
InterProiView protein in InterPro
IPR006282 Thi_PPkinase
IPR016966 Thiamin_pyrophosphokinase_euk
IPR007373 Thiamin_PyroPKinase_B1-bd
IPR036371 TPK_B1-bd_sf
IPR007371 TPK_catalytic
IPR036759 TPK_catalytic_sf
PfamiView protein in Pfam
PF04265 TPK_B1_binding, 1 hit
PF04263 TPK_catalytic, 1 hit
PIRSFiPIRSF031057 Thiamin_pyrophosphokinase, 1 hit
SMARTiView protein in SMART
SM00983 TPK_B1_binding, 1 hit
SUPFAMiSSF63862 SSF63862, 1 hit
SSF63999 SSF63999, 1 hit
TIGRFAMsiTIGR01378 thi_PPkinase, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b (identifier: P30636-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKKLKPFEI LEDSCASVCI WLNGEPTAIS NRAENLWNKA KYRVATDGAV
60 70 80 90 100
NEILKRKSFV EWPHIICGDF DSINKQIDTK NAKVVHLPDQ DYTDLSKSVQ
110 120 130 140 150
WCLEQKTLTS WEFENIVVLG GLNGRFDHTM STLSSLIRFV DSQTPVIVLD
160 170 180 190 200
SRNLVLAVPT GDSNLDVNLE MTTKMCGIIP IVQKETIVSS IGLKYEMENL
210 220 230 240
ALEFGKLIST SNEVTTSQVF LKSSSSLIFS IELENWVYKL DSL
Note: No experimental confirmation available.
Length:243
Mass (Da):27,314
Last modified:April 26, 2005 - v3
Checksum:i2BA5401B76326678
GO
Isoform a (identifier: P30636-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-160: Missing.

Show »
Length:228
Mass (Da):25,723
Checksum:i78E3536535C2CA6C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013462146 – 160Missing in isoform a. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY513235 mRNA Translation: AAS21678.1
Z11115 Genomic DNA Translation: CAA77455.3
Z11115 Genomic DNA Translation: CAI46594.1
PIRiS15797
RefSeqiNP_001023023.1, NM_001027852.1 [P30636-2]
NP_001023024.1, NM_001027853.1 [P30636-1]
UniGeneiCel.9539

Genome annotation databases

EnsemblMetazoaiZK637.9b; ZK637.9b; WBGene00014027 [P30636-1]
GeneIDi176258
KEGGicel:CELE_ZK637.9
UCSCiZK637.9b c. elegans [P30636-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTPK1_CAEEL
AccessioniPrimary (citable) accession number: P30636
Secondary accession number(s): Q5FC86, Q6R5A6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 26, 2005
Last modified: February 28, 2018
This is version 116 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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