UniProtKB - P30624 (LCF1_YEAST)
Protein
Long-chain-fatty-acid--CoA ligase 1
Gene
FAA1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It may supplement intracellular myristoyl-CoA pools from exogenous myristate. Preferentially acts on C12:0-C16:0 fatty acids with myristic and pentadecanic acid (C15:0) having the highest activities.
Miscellaneous
Present with 7470 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:6.2.1.3
Cofactori
Temperature dependencei
Optimum temperature is 30 degrees Celsius.
GO - Molecular functioni
- acyl-CoA ligase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- decanoate-CoA ligase activity Source: UniProtKB-EC
- long-chain fatty acid-CoA ligase activity Source: SGD
- medium-chain fatty acid-CoA ligase activity Source: SGD
GO - Biological processi
- long-chain fatty acid import into cell Source: SGD
- long-chain fatty acid metabolic process Source: GO_Central
- long-chain fatty-acyl-CoA metabolic process Source: SGD
- sphingoid long-chain base transport Source: SGD
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-434313, Intracellular metabolism of fatty acids regulates insulin secretion R-SCE-75876, Synthesis of very long-chain fatty acyl-CoAs |
Chemistry databases
SwissLipidsi | SLP:000000132 |
Names & Taxonomyi
Protein namesi | Recommended name: Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)Alternative name(s): Fatty acid activator 1 Long-chain acyl-CoA synthetase 1 |
Gene namesi | Name:FAA1 Ordered Locus Names:YOR317W ORF Names:O6136 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YOR317W |
SGDi | S000005844, FAA1 |
Subcellular locationi
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Mitochondrion
- mitochondrial outer membrane Source: SGD
- mitochondrion Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- lipid droplet Source: SGD
- membrane Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193119 | 1 – 700 | Long-chain-fatty-acid--CoA ligase 1Add BLAST | 700 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 189 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P30624 |
PaxDbi | P30624 |
PRIDEi | P30624 |
PTM databases
CarbonylDBi | P30624 |
iPTMneti | P30624 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34704, 147 interactors |
DIPi | DIP-4654N |
IntActi | P30624, 7 interactors |
STRINGi | 4932.YOR317W |
Miscellaneous databases
RNActi | P30624, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | KOG1180, Eukaryota |
GeneTreei | ENSGT00940000171609 |
HOGENOMi | CLU_000022_45_2_1 |
InParanoidi | P30624 |
OMAi | GEGEKMP |
Family and domain databases
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
P30624-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVAQYTVPVG KAANEHETAP RRNYQCREKP LVRPPNTKCS TVYEFVLECF
60 70 80 90 100
QKNKNSNAMG WRDVKEIHEE SKSVMKKVDG KETSVEKKWM YYELSHYHYN
110 120 130 140 150
SFDQLTDIMH EIGRGLVKIG LKPNDDDKLH LYAATSHKWM KMFLGAQSQG
160 170 180 190 200
IPVVTAYDTL GEKGLIHSLV QTGSKAIFTD NSLLPSLIKP VQAAQDVKYI
210 220 230 240 250
IHFDSISSED RRQSGKIYQS AHDAINRIKE VRPDIKTFSF DDILKLGKES
260 270 280 290 300
CNEIDVHPPG KDDLCCIMYT SGSTGEPKGV VLKHSNVVAG VGGASLNVLK
310 320 330 340 350
FVGNTDRVIC FLPLAHIFEL VFELLSFYWG ACIGYATVKT LTSSSVRNCQ
360 370 380 390 400
GDLQEFKPTI MVGVAAVWET VRKGILNQID NLPFLTKKIF WTAYNTKLNM
410 420 430 440 450
QRLHIPGGGA LGNLVFKKIR TATGGQLRYL LNGGSPISRD AQEFITNLIC
460 470 480 490 500
PMLIGYGLTE TCASTTILDP ANFELGVAGD LTGCVTVKLV DVEELGYFAK
510 520 530 540 550
NNQGEVWITG ANVTPEYYKN EEETSQALTS DGWFKTGDIG EWEANGHLKI
560 570 580 590 600
IDRKKNLVKT MNGEYIALEK LESVYRSNEY VANICVYADQ SKTKPVGIIV
610 620 630 640 650
PNHAPLTKLA KKLGIMEQKD SSINIENYLE DAKLIKAVYS DLLKTGKDQG
660 670 680 690 700
LVGIELLAGI VFFDGEWTPQ NGFVTSAQKL KRKDILNAVK DKVDAVYSSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66194 Genomic DNA Translation: CAA46957.1 X90565 Genomic DNA Translation: CAA62172.1 Z75225 Genomic DNA Translation: CAA99637.1 BK006948 Genomic DNA Translation: DAA11082.1 |
PIRi | S23052 |
RefSeqi | NP_014962.3, NM_001183737.3 |
Genome annotation databases
EnsemblFungii | YOR317W_mRNA; YOR317W; YOR317W |
GeneIDi | 854495 |
KEGGi | sce:YOR317W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66194 Genomic DNA Translation: CAA46957.1 X90565 Genomic DNA Translation: CAA62172.1 Z75225 Genomic DNA Translation: CAA99637.1 BK006948 Genomic DNA Translation: DAA11082.1 |
PIRi | S23052 |
RefSeqi | NP_014962.3, NM_001183737.3 |
3D structure databases
SMRi | P30624 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34704, 147 interactors |
DIPi | DIP-4654N |
IntActi | P30624, 7 interactors |
STRINGi | 4932.YOR317W |
Chemistry databases
SwissLipidsi | SLP:000000132 |
PTM databases
CarbonylDBi | P30624 |
iPTMneti | P30624 |
Proteomic databases
MaxQBi | P30624 |
PaxDbi | P30624 |
PRIDEi | P30624 |
Genome annotation databases
EnsemblFungii | YOR317W_mRNA; YOR317W; YOR317W |
GeneIDi | 854495 |
KEGGi | sce:YOR317W |
Organism-specific databases
EuPathDBi | FungiDB:YOR317W |
SGDi | S000005844, FAA1 |
Phylogenomic databases
eggNOGi | KOG1180, Eukaryota |
GeneTreei | ENSGT00940000171609 |
HOGENOMi | CLU_000022_45_2_1 |
InParanoidi | P30624 |
OMAi | GEGEKMP |
Enzyme and pathway databases
Reactomei | R-SCE-434313, Intracellular metabolism of fatty acids regulates insulin secretion R-SCE-75876, Synthesis of very long-chain fatty acyl-CoAs |
Miscellaneous databases
PROi | PR:P30624 |
RNActi | P30624, protein |
Family and domain databases
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LCF1_YEAST | |
Accessioni | P30624Primary (citable) accession number: P30624 Secondary accession number(s): D6W316 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD